(Circular) (Six-base) MAPSORT of: pFN21AE3202.seq Check: 3644 from: 1 to: 6118 pFN21AE3202 6118 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3461 1937 Size: 790 734 4594 Fragments arranged by size: 4594 790 734 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2921 3066 4181 5565 5833 1632 Size: 731 558 145 1115 1384 268 1917 Fragments arranged by size: 1917 1384 1115 731 558 268 145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 2135 3284 1166 Size: 969 1149 4000 Fragments arranged by size: 4000 1149 969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 1184 Size: 915 5203 AlwNI CAG_nnn'CTG Cuts at: 3127 3861 5433 3127 Size: 734 1572 3812 Fragments arranged by size: 3812 1572 734 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2279 3050 3427 3653 3805 5914 1493 Size: 786 771 377 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 771 377 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2160 3375 3443 1870 Size: 73 217 1215 68 4545 Fragments arranged by size: 4545 1215 217 73 68 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3198 3439 4894 618 Size: 325 217 627 1411 241 1455 1842 Fragments arranged by size: 1842 1455 1411 627 325 241 217 BanII G_rGCy'C Cuts at: 1391 2028 2102 3437 1391 Size: 637 74 1335 4072 Fragments arranged by size: 4072 1335 637 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2583 928 Size: 971 684 4463 Fragments arranged by size: 4463 971 684 BbvCI CC'TCA_GC Cuts at: 2943 2943 Size: 6118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3391 4010 5220 3391 Size: 619 1210 4289 Fragments arranged by size: 4289 1210 619 BclI T'GATC_A Cuts at: 1548 2139 1548 Size: 591 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2781 4729 136 Size: 107 122 71 631 605 1109 1948 1525 Fragments arranged by size: 1948 1525 1109 631 605 122 107 71 BglII A'GATC_T Cuts at: 2049 2661 6113 2049 Size: 612 3452 2054 Fragments arranged by size: 3452 2054 612 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2678 3257 4179 5335 1790 Size: 888 579 922 1156 2573 Fragments arranged by size: 2573 1156 922 888 579 BmgBI CAC'GTC Cuts at: 1318 2671 1318 Size: 1353 4765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 3849 4807 Size: 416 53 83 399 24 690 1742 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1753 1742 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1995 3149 4778 1239 Size: 756 1154 1629 2579 Fragments arranged by size: 2579 1629 1154 756 Bpu10I CC'TnA_GC Cuts at: 2943 2943 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3233 4243 5108 5406 3233 Size: 1010 865 298 3945 Fragments arranged by size: 3945 1010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3057 4781 882 Size: 617 1558 1724 2219 Fragments arranged by size: 2219 1724 1558 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 3075 6112 3075 Size: 3037 3081 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3023 1186 Size: 1837 4281 BseYI C'CCAG_C Cuts at: 3406 5321 5817 3406 Size: 1915 496 3707 Fragments arranged by size: 3707 1915 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 3245 1163 Size: 573 984 525 4036 Fragments arranged by size: 4036 984 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 3273 4179 4264 5335 2028 Size: 1245 906 85 1071 2811 Fragments arranged by size: 2811 1245 1071 906 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2102 2678 3257 3273 3437 4179 Size: 399 238 74 576 579 16 164 742 Cuts at: 4179 4264 5335 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 576 399 238 164 85 74 16 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 2736 4007 5737 2736 Size: 1271 1730 3117 Fragments arranged by size: 3117 1730 1271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 3268 794 Size: 2474 3644 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 2049 2661 3112 3448 3815 4200 4217 5669 Size: 77 612 451 336 367 385 17 1452 Cuts at: 5669 5755 5767 6113 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 612 451 385 367 346 336 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 3384 3384 Size: 6118 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5566 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2330 2975 3056 3634 4428 4448 2265 Size: 65 645 81 578 794 20 3935 Fragments arranged by size: 3935 794 645 578 81 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DraIII CAC_nnn'GTG Cuts at: 3268 3268 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 3565 4454 Size: 54 1425 50 275 18 1035 700 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1035 889 700 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2918 4048 1353 Size: 1565 1130 3423 Fragments arranged by size: 3423 1565 1130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2026 Size: 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2363 2921 3066 3149 4181 4778 Size: 393 363 368 558 145 83 1032 597 Cuts at: 4778 5565 5833 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 597 558 393 368 363 268 145 83 EcoO109I rG'GnC_Cy Cuts at: 2947 2977 2947 Size: 30 6088 EcoRI G'AATT_C Cuts at: 2279 3427 3805 2279 Size: 1148 378 4592 Fragments arranged by size: 4592 1148 378 EcoRV GAT'ATC Cuts at: 2198 2198 Size: 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2546 2546 Size: 6118 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2796 2828 4759 4791 4833 Size: 32 135 32 1224 32 1931 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 1931 1224 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2611 3462 3708 669 Size: 988 281 673 851 246 3079 Fragments arranged by size: 3079 988 851 673 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2867 10 Size: 54 1425 343 1035 3261 Fragments arranged by size: 3261 1425 1035 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3284 4076 4435 4594 518 Size: 648 969 1149 792 359 159 2042 Fragments arranged by size: 2042 1149 969 792 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2942 3534 4211 5359 5604 1606 Size: 464 872 592 677 1148 245 2120 Fragments arranged by size: 2120 1148 872 677 592 464 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5566 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2884 2950 1348 Size: 1536 66 4516 Fragments arranged by size: 4516 1536 66 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 1108 Size: 1515 4603 PfoI T'CCnGG_A Cuts at: 3030 3216 3030 Size: 186 5932 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspXI vC'TCGA_Gb Cuts at: 2160 2160 Size: 6118 PstI C_TGCA'G Cuts at: 830 2439 3470 830 Size: 1609 1031 3478 Fragments arranged by size: 3478 1609 1031 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2942 2942 Size: 6118 SacI G_AGCT'C Cuts at: 2028 2028 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SexAI A'CCwGG_T Cuts at: 2036 2036 Size: 6118 SfcI C'TryA_G Cuts at: 826 1045 2435 3466 4601 5282 5473 826 Size: 219 1390 1031 1135 681 191 1471 Fragments arranged by size: 1471 1390 1135 1031 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3377 3445 1872 Size: 1505 68 4545 Fragments arranged by size: 4545 1505 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 3248 4222 5123 5385 820 Size: 197 926 217 1088 974 901 262 1553 Fragments arranged by size: 1553 1088 974 926 901 262 217 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2622 3093 3557 513 Size: 552 1557 471 464 3074 Fragments arranged by size: 3074 1557 552 471 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2942 4145 4162 4315 4500 4945 Size: 1255 465 897 1203 17 153 185 445 Cuts at: 4945 325 Size: 1498 Fragments arranged by size: 1498 1255 1203 897 465 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3166 4364 6076 Size: 274 80 33 51 1443 1189 1198 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 1712 1443 1198 1189 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 2160 1943 Size: 217 5901 XmaI C'CCGG_G Cuts at: 1870 3375 3443 1870 Size: 1505 68 4545 Fragments arranged by size: 4545 1505 68 XmnI GAAnn'nnTTC Cuts at: 2283 2568 2580 4247 2283 Size: 285 12 1667 4154 Fragments arranged by size: 4154 1667 285 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI