################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BamHI BbrPI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI MfeI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 461 ACGTGT A^C[GA][CT]GT Asp700I 240 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 44 TGCGCA TGC^GCA BalI 851 TGGCCA TGG^CCA BbsI 472 GAAGAC GAAGAC(2/6) BbvCI 870 CCTCAGC CCTCAGC(-5/-2) BcgI 126 GCAGAGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1064 TGATCA T^GATCA BfaI 722 CTAG C^TAG BpmI 525 CTCCAG CTGGAG(16/14) BpuAI 472 GAAGAC GAAGAC(2/6) BsaAI 460 TACGTG [CT]AC^GT[GA] BsaI 340 GAGACC GGTCTC(1/5) BsiEI 1179 CGGCCG CG[GA][CT]^CG BspMI 385 GCAGGT ACCTGC(4/8) BsrDI 1068 CATTGC GCAATG(2/0) BstBI 64 TTCGAA TT^CGAA BstYI 77 AGATCC [GA]^GATC[CT] DraII 866 AGGCCCT [GA]G^G[ACGT]CC[CT] EagI 1179 CGGCCG C^GGCCG EclXI 1179 CGGCCG C^GGCCG Eco52I 1179 CGGCCG C^GGCCG EcoNI 837 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 866 AGGCCCT [GA]G^G[ACGT]CC[CT] FbaI 1064 TGATCA T^GATCA FspI 44 TGCGCA TGC^GCA HgaI 319 GACGC GACGC(5/10) HincII 907 GTTGAC GT[CT]^[GA]AC HindII 907 GTTGAC GT[CT]^[GA]AC MaeI 722 CTAG C^TAG MaeIII 603 GTCAC ^GT[ACGT]AC MflI 77 AGATCC [GA]^GATC[CT] MluNI 851 TGGCCA TGG^CCA MscI 851 TGGCCA TGG^CCA NaeI 829 GCCGGC GCC^GGC NcoI 541 CCATGG C^CATGG NgoMIV 829 GCCGGC G^CCGGC NlaIII 542 CATG CATG^ NspV 64 TTCGAA TT^CGAA PstI 622 CTGCAG CTGCA^G SfaNI 173 GCATC GCATC(5/9) SfcI 622 CTGCAG C^T[GA][CT]AG SfuI 64 TTCGAA TT^CGAA StuI 961 AGGCCT AGG^CCT Tsp45I 603 GTCAC ^GT[GC]AC XcmI 815 CCATCCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 77 AGATCC [GA]^GATC[CT] XmnI 240 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 298 GGCGCC G[GA]^CG[CT]C 375 GGCGCC AlwI 78 GATCC GGATC(4/5) 84 GATCC AvaII 486 GGACC G^G[AT]CC 630 GGTCC BbeI 298 GGCGCC GGCGC^C 375 GGCGCC BglI 4 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 700 GCCCAAGTGGC BsaBI 78 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1184 GATGGAAATC BsaHI 298 GGCGCC G[GA]^CG[CT]C 375 GGCGCC BsmFI 167 GGGAC GGGAC(10/14) 846 GGGAC BsrBI 1114 CCGCTC CCGCTC(-3/-3) 151 GAGCGG BssHII 96 GCGCGC G^CGCGC 122 GCGCGC BstF5I 354 CATCC GGATG(2/0) 816 CATCC FokI 354 CATCC GGATG(9/13) 816 CATCC Hin1I 298 GGCGCC G[GA]^CG[CT]C 375 GGCGCC KasI 298 GGCGCC G^GCGCC 375 GGCGCC KspI 311 CCGCGG CCGC^GG 329 CCGCGG MaeII 461 ACGT A^CGT 492 ACGT MamI 78 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1184 GATGGAAATC NarI 298 GGCGCC GG^CGCC 375 GGCGCC PvuII 201 CAGCTG CAG^CTG 634 CAGCTG SacII 311 CCGCGG CCGC^GG 329 CCGCGG SfoI 298 GGCGCC GGC^GCC 375 GGCGCC TaqI 65 TCGA T^CGA 840 TCGA TthHB8I 65 TCGA T^CGA 840 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AluI AlwNI Aor51HI ApaI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI Eco47III EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI StyI TseI TspRI