(Circular) (Six-base) MAPSORT of: pFN21AE3169.seq Check: 2593 from: 1 to: 5902 pFN21AE3169 5902 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2381 2381 Size: 5902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3965 5349 5617 1632 Size: 2333 1384 268 1917 Fragments arranged by size: 2333 1917 1384 268 AfeI AGC'GCT Cuts at: 2240 2462 2881 2240 Size: 222 419 5261 Fragments arranged by size: 5261 419 222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 2466 4801 5778 1203 Size: 1263 2335 977 1327 Fragments arranged by size: 2335 1327 1263 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2826 1184 Size: 1642 4260 AlwNI CAG_nnn'CTG Cuts at: 2086 2396 2854 3645 5217 2086 Size: 310 458 791 1572 2771 Fragments arranged by size: 2771 1572 791 458 310 ApaI G_GGCC'C Cuts at: 2313 2554 2800 2313 Size: 241 246 5415 Fragments arranged by size: 5415 246 241 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 3211 3437 3589 5698 1493 Size: 1718 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 3227 1870 Size: 73 1284 4545 Fragments arranged by size: 4545 1284 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2380 2713 2837 3097 Size: 325 217 627 516 77 333 124 260 Cuts at: 3097 3223 4678 618 Size: 126 1455 1842 Fragments arranged by size: 1842 1455 627 516 333 325 260 217 126 124 77 BanII G_rGCy'C Cuts at: 1391 2313 2412 2531 2554 2800 3015 3221 1391 Size: 922 99 119 23 246 215 206 4072 Fragments arranged by size: 4072 922 246 215 206 119 99 23 BbeI G_GCGC'C Cuts at: 2307 2384 2307 Size: 77 5825 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 928 Size: 971 585 4346 Fragments arranged by size: 4346 971 585 BbvCI CC'TCA_GC Cuts at: 2876 2876 Size: 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 4093 4127 1903 Size: 34 183 34 1939 34 3678 Fragments arranged by size: 3678 1939 183 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 3069 1548 Size: 1521 4381 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2711 4513 Size: 107 122 71 631 605 343 696 1802 Cuts at: 4513 136 Size: 1525 Fragments arranged by size: 1802 1525 696 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 2313 2554 2800 3100 3963 5119 1790 Size: 523 241 246 300 863 1156 2573 Fragments arranged by size: 2573 1156 863 523 300 246 241 BmgBI CAC'GTC Cuts at: 1318 2498 1318 Size: 1180 4722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2903 3633 4591 Size: 416 53 83 399 24 1486 730 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1486 958 730 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2515 4562 1239 Size: 1276 2047 2579 Fragments arranged by size: 2579 2047 1276 Bpu10I CC'TnA_GC Cuts at: 2876 2876 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4565 882 Size: 617 840 2226 2219 Fragments arranged by size: 2226 2219 840 617 BsaAI yAC'GTr Cuts at: 493 1204 2467 3693 493 Size: 711 1263 1226 2702 Fragments arranged by size: 2702 1263 1226 711 BsaBI GATnn'nnATC Cuts at: 2087 3193 5896 2087 Size: 1106 2703 2093 Fragments arranged by size: 2703 2093 1106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 2381 3709 4091 Size: 53 83 186 936 771 77 1328 382 Cuts at: 4091 4726 275 Size: 635 1451 Fragments arranged by size: 1451 1328 936 771 635 382 186 83 77 53 BsaWI w'CCGG_w Cuts at: 3199 4335 4733 5007 5154 5565 3199 Size: 1136 398 274 147 411 3536 Fragments arranged by size: 3536 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2591 2621 3145 Size: 30 165 30 912 30 246 30 524 Cuts at: 3145 3175 1178 Size: 30 3905 Fragments arranged by size: 3905 912 524 246 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 2420 2733 3104 1186 Size: 1171 63 313 371 3984 Fragments arranged by size: 3984 1171 371 313 63 BseYI C'CCAG_C Cuts at: 2396 2457 2671 2799 3112 5105 5601 2396 Size: 61 214 128 313 1993 496 2697 Fragments arranged by size: 2697 1993 496 313 214 128 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 2520 2548 1163 Size: 573 516 268 28 4517 Fragments arranged by size: 4517 573 516 268 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3352 4113 5141 1540 Size: 275 186 1186 165 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 165 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2412 2531 2554 2800 3015 3100 Size: 399 523 99 119 23 246 215 85 Cuts at: 3100 3221 3963 4048 5119 1391 Size: 121 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 523 399 246 215 121 119 99 85 85 23 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 3257 844 Size: 1537 876 3489 Fragments arranged by size: 3489 1537 876 BsrBI CCG'CTC Cuts at: 1394 1823 2158 3121 3789 1394 Size: 429 335 963 668 3507 Fragments arranged by size: 3507 963 668 429 335 BsrDI GCAATG_nn' Cuts at: 65 1248 3072 4397 4571 65 Size: 1183 1824 1325 174 1396 Fragments arranged by size: 1824 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2632 2834 2983 4546 4733 1700 Size: 110 822 202 149 1563 187 2869 Fragments arranged by size: 2869 1563 822 202 187 149 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 2101 2127 1925 Size: 176 26 5700 Fragments arranged by size: 5700 176 26 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 2070 4706 2070 Size: 2636 3266 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2082 3232 3599 3984 4001 5453 5539 5551 Size: 110 1150 367 385 17 1452 86 12 Cuts at: 5551 5897 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1150 385 367 346 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2316 2334 2546 2588 3021 513 Size: 552 939 312 18 212 42 433 3394 Fragments arranged by size: 3394 939 552 433 312 212 42 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2164 487 Size: 683 56 938 4225 Fragments arranged by size: 4225 938 683 56 BtsI GCAGTG_nn' Cuts at: 2681 3054 3418 4212 4232 2681 Size: 373 364 794 20 4351 Fragments arranged by size: 4351 794 373 364 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2832 2856 3184 Size: 54 1425 50 275 18 1002 24 328 Cuts at: 3184 3349 4238 5867 8 Size: 165 889 1629 43 Fragments arranged by size: 1629 1425 1002 889 328 275 165 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 3349 1537 Size: 275 1372 165 4090 Fragments arranged by size: 4090 1372 275 165 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2334 2431 3832 1353 Size: 714 267 97 1401 3423 Fragments arranged by size: 3423 1401 714 267 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2417 4489 4863 5009 387 Size: 1064 138 828 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 828 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2515 3965 4562 5349 5617 1239 Size: 393 883 1450 597 787 268 1524 Fragments arranged by size: 1524 1450 883 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2846 2846 Size: 5902 EcoO109I rG'GnC_Cy Cuts at: 2872 2872 Size: 5902 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 5902 FspI TGC'GCA Cuts at: 2051 4408 2051 Size: 2357 3545 HaeII r_GCGC'y Cuts at: 1678 2242 2260 2307 2384 2451 2464 2883 5049 Size: 564 18 47 77 67 13 419 2166 Cuts at: 5049 1678 Size: 2531 Fragments arranged by size: 2531 2166 564 419 77 67 47 18 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2591 2623 3002 Size: 32 135 32 743 32 244 32 379 Cuts at: 3002 3034 4543 4575 4617 4649 1373 Size: 32 1509 32 42 32 2626 Fragments arranged by size: 2626 1509 743 379 244 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2914 3246 3492 669 Size: 988 281 976 332 246 3079 Fragments arranged by size: 3079 988 976 332 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KasI G'GCGC_C Cuts at: 2303 2380 2303 Size: 77 5825 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2454 5016 5200 1305 Size: 1149 2562 184 2007 Fragments arranged by size: 2562 2007 1149 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2858 10 Size: 54 1425 343 1026 3054 Fragments arranged by size: 3054 1425 1026 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2208 2318 2336 2641 3318 3995 5143 5388 Size: 602 110 18 305 677 677 1148 245 Cuts at: 5388 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 677 602 305 245 110 18 NaeI GCC'GGC Cuts at: 2836 2836 Size: 5902 NarI GG'CG_CC Cuts at: 2304 2381 2304 Size: 77 5825 NcoI C'CATG_G Cuts at: 513 1065 2004 2546 513 Size: 552 939 542 3869 Fragments arranged by size: 3869 939 552 542 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NgoMIV G'CCGG_C Cuts at: 2834 2834 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PfoI T'CCnGG_A Cuts at: 2823 2823 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PspOMI G'GGCC_C Cuts at: 2309 2550 2796 2309 Size: 241 246 5415 Fragments arranged by size: 5415 246 241 PstI C_TGCA'G Cuts at: 830 2631 3254 830 Size: 1801 623 3478 Fragments arranged by size: 3478 1801 623 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2208 2641 2208 Size: 433 5469 SacII CC_GC'GG Cuts at: 2319 2337 2319 Size: 18 5884 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2627 3250 4385 5066 5257 826 Size: 219 1582 623 1135 681 191 1471 Fragments arranged by size: 1582 1471 1135 681 623 219 191 SfoI GGC'GCC Cuts at: 2305 2382 2305 Size: 77 5825 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 2968 1517 Size: 1451 4451 StyI C'CwwG_G Cuts at: 513 1065 2004 2265 2546 2595 2991 3080 3341 Size: 552 939 261 281 49 396 89 261 Cuts at: 3341 513 Size: 3074 Fragments arranged by size: 3074 939 552 396 281 261 261 89 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2508 2770 3929 3946 4099 4284 4729 Size: 1255 928 262 1159 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1498 1255 1159 928 445 262 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2827 2827 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 2249 4031 2249 Size: 1782 4120 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI