################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BmyI BsaAI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI KspI MluI MluNI MroI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 1289 GACGCC G[GA]^CG[CT]C AhdI 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1188 CCTGAGG CC^T[ACGT]AGG AspEI 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcnI 1178 CCCGG CC^[GC]GG BpmI 847 CTCCAG CTGGAG(16/14) BsaBI 1390 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1289 GACGCC G[GA]^CG[CT]C BsaI 304 GAGACC GGTCTC(1/5) BseRI 520 GAGGAG GAGGAG(10/8) Bsp1407I 1454 TGTACA T^GTACA BspHI 717 TCATGA T^CATGA BsrGI 1454 TGTACA T^GTACA BssSI 1474 CTCGTG CACGAG(-5/-1) BstAPI 1350 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 1322 GGATG GGATG(2/0) Bsu36I 1188 CCTGAGG CC^T[ACGT]AGG CfoI 1489 GCGC GCG^C DraII 116 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 445 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1188 CCTGAGG CC^T[ACGT]AGG EcoO109I 116 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 671 GAATTC G^AATTC EcoRV 713 GATATC GAT^ATC FauI 1596 CCCGC CCCGC(4/6) FokI 1322 GGATG GGATG(9/13) HapII 1179 CCGG C^CGG HhaI 1489 GCGC GCG^C Hin1I 1289 GACGCC G[GA]^CG[CT]C HinP1I 1489 GCGC G^CGC HpaII 1179 CCGG C^CGG MamI 1390 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 200 CAATTG C^AATTG MspI 1179 CCGG C^CGG MunI 200 CAATTG C^AATTG NciI 1178 CCCGG CC^[GC]GG PaeR7I 1297 CTCGAG C^TCGAG PpuMI 116 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1408 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 717 TCATGA T^CATGA ScaI 131 AGTACT AGT^ACT SmlI 1297 CTCGAG C^T[CT][GA]AG SspBI 1454 TGTACA T^GTACA SspI 791 AATATT AAT^ATT XhoI 1297 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 492 CGCG CG^CG 1341 CGCG AcsI 554 AAATTC [GA]^AATT[CT] 671 GAATTC AlwNI 967 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1351 CAGATTCTG ApoI 554 AAATTC [GA]^AATT[CT] 671 GAATTC Asp700I 498 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1687 GAAGACATTC AvaI 1297 CTCGAG C^[CT]CG[GA]G 1561 CCCGAG BamHI 615 GGATCC G^GATCC 1040 GGATCC BlpI 124 GCTAAGC GC^T[ACGT]AGC 1359 GCTCAGC Bpu1102I 124 GCTAAGC GC^T[ACGT]AGC 1359 GCTCAGC BsaJI 1169 CCCAGG C^C[ACGT][ACGT]GG 1430 CCCAGG BsmFI 1593 GTCCC GGGAC(10/14) 1608 GTCCC BsmI 148 GAATGC GAATGC(1/-1) 892 GAATGC BsoBI 1297 CTCGAG C^[CT]CG[GA]G 1561 CCCGAG BstUI 492 CGCG CG^CG 1341 CGCG BstYI 615 GGATCC [GA]^GATC[CT] 1040 GGATCC CelII 124 GCTAAGC GC^T[ACGT]AGC 1359 GCTCAGC DraI 940 TTTAAA TTT^AAA 1108 TTTAAA EarI 484 GAAGAG CTCTTC(1/4) 517 GAAGAG Ksp632I 484 GAAGAG CTCTTC(1/4) 517 GAAGAG MaeIII 869 GTTAC ^GT[ACGT]AC 950 GTTAC MflI 615 GGATCC [GA]^GATC[CT] 1040 GGATCC MvnI 492 CGCG CG^CG 1341 CGCG SfaNI 904 GATGC GCATC(5/9) 1468 GATGC TaqI 254 TCGA T^CGA 1298 TCGA TthHB8I 254 TCGA T^CGA 1298 TCGA XhoII 615 GGATCC [GA]^GATC[CT] 1040 GGATCC XmnI 498 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1687 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbsI BbvI BfaI BpuAI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HgaI HinfI ItaI MaeI MaeII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI