(Circular) (Six-base) MAPSORT of: pFN21AB6106.seq Check: 9998 from: 1 to: 6508 pFN21AB6106 6508 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3851 1937 Size: 1914 4594 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 3807 4635 5008 1563 Size: 2244 828 373 3063 Fragments arranged by size: 3063 2244 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 4571 5955 6223 1632 Size: 876 2063 1384 268 1917 Fragments arranged by size: 2063 1917 1384 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 5407 6384 1203 Size: 4204 977 1327 Fragments arranged by size: 4204 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 3595 5237 3595 Size: 1642 4866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 1184 Size: 1653 4855 AlwNI CAG_nnn'CTG Cuts at: 2977 3361 4251 5823 2977 Size: 384 890 1572 3662 Fragments arranged by size: 3662 1572 890 384 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2559 2676 3817 4043 4195 6304 1493 Size: 1066 117 1141 226 152 2109 1697 Fragments arranged by size: 2109 1697 1141 1066 226 152 117 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 3302 3566 3833 1870 Size: 73 1359 264 267 4545 Fragments arranged by size: 4545 1359 267 264 73 BamHI G'GATC_C Cuts at: 2620 3045 3838 4205 2620 Size: 425 793 367 4923 Fragments arranged by size: 4923 793 425 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3829 5284 618 Size: 325 217 627 2042 1455 1842 Fragments arranged by size: 2042 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3827 1391 Size: 2436 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 3594 3663 3699 3720 928 Size: 971 162 1533 69 36 21 3716 Fragments arranged by size: 3716 1533 971 162 69 36 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5119 136 Size: 107 122 71 631 605 3447 1525 Fragments arranged by size: 3447 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6503 6503 Size: 6508 BlpI GC'TnA_GC Cuts at: 2130 3365 3925 2130 Size: 1235 560 4713 Fragments arranged by size: 4713 1235 560 Bme1580I G_kGCm'C Cuts at: 1790 4569 5725 1790 Size: 2779 1156 2573 Fragments arranged by size: 2779 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3104 3612 1318 Size: 1786 508 4214 Fragments arranged by size: 4214 1786 508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 4239 5197 Size: 416 53 83 399 24 618 2204 958 Cuts at: 5197 442 Size: 1753 Fragments arranged by size: 2204 1753 958 618 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2837 5168 1239 Size: 1598 2331 2579 Fragments arranged by size: 2579 2331 1598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 5171 882 Size: 617 804 2868 2219 Fragments arranged by size: 2868 2219 804 617 BsaAI yAC'GTr Cuts at: 493 1204 4299 493 Size: 711 3095 2702 Fragments arranged by size: 3095 2702 711 BsaBI GATnn'nnATC Cuts at: 3399 6502 3399 Size: 3103 3405 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 4315 4697 5332 Size: 53 83 186 936 1762 1020 382 635 Cuts at: 5332 275 Size: 1451 Fragments arranged by size: 1762 1451 1020 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6283 Fragments arranged by size: 6283 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 1186 Size: 1354 5154 BseYI C'CCAG_C Cuts at: 3351 5711 6207 3351 Size: 2360 496 3652 Fragments arranged by size: 3652 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5935 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3958 4719 5747 1540 Size: 275 186 1957 761 1028 2301 Fragments arranged by size: 2301 1957 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4569 4654 5725 4569 Size: 85 1071 5352 Fragments arranged by size: 5352 1071 85 BsmI GAATG_Cn' Cuts at: 2159 2903 4019 4112 2159 Size: 744 1116 93 4555 Fragments arranged by size: 4555 1116 744 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3827 4569 4654 5725 1391 Size: 399 2037 742 85 1071 2174 Fragments arranged by size: 2174 2037 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 2722 4397 6127 2722 Size: 1675 1730 3103 Fragments arranged by size: 3103 1730 1675 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3863 844 Size: 3019 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4395 1394 Size: 429 2572 3507 Fragments arranged by size: 3507 2572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5003 5177 65 Size: 1183 3755 174 1396 Fragments arranged by size: 3755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5152 5339 1700 Size: 110 3342 187 2869 Fragments arranged by size: 3342 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3459 96 Size: 3363 3145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 3479 4568 5580 3479 Size: 1089 1012 4407 Fragments arranged by size: 4407 1089 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3361 794 Size: 2567 3941 BstBI TT'CG_AA Cuts at: 5312 5312 Size: 6508 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2620 3045 3838 4205 4590 4607 6059 6145 Size: 648 425 793 367 385 17 1452 86 Cuts at: 6145 6157 6503 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 793 648 425 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3194 3194 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2477 487 Size: 683 56 1251 4518 Fragments arranged by size: 4518 1251 683 56 BtsI GCAGTG_nn' Cuts at: 4024 4818 4838 4024 Size: 794 20 5694 Fragments arranged by size: 5694 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6473 DraI TTT'AAA Cuts at: 2947 3115 3812 2947 Size: 168 697 5643 Fragments arranged by size: 5643 697 168 DraIII CAC_nnn'GTG Cuts at: 2455 2455 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3225 3483 3525 Size: 54 1425 50 275 18 1395 258 42 Cuts at: 3525 3955 4844 6473 8 Size: 430 889 1629 43 Fragments arranged by size: 1629 1425 1395 889 430 275 258 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3955 1537 Size: 275 2143 4090 Fragments arranged by size: 4090 2143 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2484 2517 4438 1353 Size: 1131 33 1921 3423 Fragments arranged by size: 3423 1921 1131 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2870 5095 5469 5615 387 Size: 1064 138 1281 2225 374 146 1280 Fragments arranged by size: 2225 1281 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2837 4571 5168 5955 6223 1239 Size: 393 876 329 1734 597 787 268 1524 Fragments arranged by size: 1734 1524 876 787 597 393 329 268 EcoO109I rG'GnC_Cy Cuts at: 2122 2122 Size: 6508 EcoRI G'AATT_C Cuts at: 2676 3817 4195 2676 Size: 1141 378 4989 Fragments arranged by size: 4989 1141 378 EcoRV GAT'ATC Cuts at: 2720 2720 Size: 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3203 3737 3203 Size: 534 5974 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 5655 1678 Size: 3977 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2307 2339 3285 3317 3501 Size: 32 135 32 735 32 946 32 184 Cuts at: 3501 3526 3533 3558 3581 3613 3752 3784 5149 Size: 25 7 25 23 32 139 32 1365 Cuts at: 5149 5181 5223 5255 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1365 946 735 184 139 135 42 32 32 32 32 32 32 32 32 25 25 23 7 HincII GTy'rAC Cuts at: 669 1657 1938 3852 4098 669 Size: 988 281 1914 246 3079 Fragments arranged by size: 3079 1914 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 2205 4107 2205 Size: 1902 4606 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 2763 5622 5806 1305 Size: 981 477 2859 184 2007 Fragments arranged by size: 2859 2007 981 477 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4686 Fragments arranged by size: 4686 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4466 4825 4984 518 Size: 648 3300 359 159 2042 Fragments arranged by size: 3300 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2857 2893 3523 3577 3924 4601 5749 5994 Size: 1251 36 630 54 347 677 1148 245 Cuts at: 5994 1606 Size: 2120 Fragments arranged by size: 2120 1251 1148 677 630 347 245 54 36 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 3866 5411 1132 Size: 97 2637 1545 2229 Fragments arranged by size: 2637 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6508 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6508 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2122 Size: 6508 PshAI GACnn'nnGTC Cuts at: 1613 3417 1613 Size: 1804 4704 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PstI C_TGCA'G Cuts at: 830 3860 830 Size: 3030 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2857 2893 3523 2857 Size: 36 630 5842 Fragments arranged by size: 5842 630 36 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 2138 4756 6468 2138 Size: 2618 1712 2178 Fragments arranged by size: 2618 2178 1712 SfcI C'TryA_G Cuts at: 826 1045 3856 4991 5672 5863 826 Size: 219 2811 1135 681 191 1471 Fragments arranged by size: 2811 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3835 1872 Size: 1963 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3302 4612 5513 5775 820 Size: 197 926 1359 1310 901 262 1553 Fragments arranged by size: 1553 1359 1310 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 2798 4432 5 Size: 47 2746 1634 2081 Fragments arranged by size: 2746 2081 1634 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6508 StyI C'CwwG_G Cuts at: 513 1065 2004 3947 513 Size: 552 939 1943 3074 Fragments arranged by size: 3074 1943 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3465 4535 4552 4705 4890 5335 325 Size: 1255 1885 1070 17 153 185 445 1498 Fragments arranged by size: 1885 1498 1255 1070 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 3459 4754 Size: 274 80 33 51 1443 159 1323 1295 Cuts at: 4754 6466 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1323 1295 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2852 2884 6468 6500 2852 Size: 32 3584 32 2860 Fragments arranged by size: 3584 2860 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XhoI C'TCGA_G Cuts at: 1943 3302 1943 Size: 1359 5149 XmaI C'CCGG_G Cuts at: 1870 3833 1870 Size: 1963 4545 XmnI GAAnn'nnTTC Cuts at: 2507 3696 4637 2507 Size: 1189 941 4378 Fragments arranged by size: 4378 1189 941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI