Restriction map for
pFN21AE3476
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp718I AvrII BamHI BcgI
BciVI BclI BfrI BlnI BsaBI
BsiWI BsmI BspLU11I BsrDI Bst1107I
BstZ17I CpoI DraI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MseI MunI NdeI NotI
NruI NsiI NspI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SexAI SnaBI
SpeI SphI SspI SwaI Tru9I
XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 4341 ACACGT A^C[GA][CT]GT
Alw44I 3123 GTGCAC G^TGCAC
Aor51HI 2241 AGCGCT AGC^GCT
ApaLI 3123 GTGCAC G^TGCAC
Asp700I 2608 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2612 GTCTTC GAAGAC(2/6)
BpuAI 2612 GTCTTC GAAGAC(2/6)
Bsp1407I 3233 TGTACA T^GTACA
BspDI 4009 ATCGAT AT^CGAT
BspHI 863 TCATGA T^CATGA
BsrGI 3233 TGTACA T^GTACA
BstBI 1009 TTCGAA TT^CGAA
ClaI 4009 ATCGAT AT^CGAT
DrdI 4530 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 231 CGGCCG C^GGCCG
EclXI 231 CGGCCG C^GGCCG
Eco47III 2241 AGCGCT AGC^GCT
Eco52I 231 CGGCCG C^GGCCG
NheI 1609 GCTAGC G^CTAGC
NspV 1009 TTCGAA TT^CGAA
PaeR7I 3527 CTCGAG C^TCGAG
PflFI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 863 TCATGA T^CATGA
SapI 958 GAAGAGC GCTCTTC(1/4)
SfuI 1009 TTCGAA TT^CGAA
SgrAI 3726 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 3233 TGTACA T^GTACA
Tsp509I 4637 AATT ^AATT
Tth111I 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3527 CTCGAG C^TCGAG
XmnI 2608 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 992 TCCGGA T^CCGGA
2453 TCCGGA
AhdI 772 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACCAAGTGTC
AspEI 772 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACCAAGTGTC
AviII 2555 TGCGCA TGC^GCA
3356 TGCGCA
BbrPI 3120 CACGTG CAC^GTG
4342 CACGTG
BseAI 992 TCCGGA T^CCGGA
2453 TCCGGA
BsiEI 231 CGGCCG CG[GA][CT]^CG
3705 CGACCG
BspEI 992 TCCGGA T^CCGGA
2453 TCCGGA
BssHII 15 GCGCGC G^CGCGC
17 GCGCGC
BstEII 252 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
BstPI 252 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
Eam1105I 772 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACCAAGTGTC
EcoO65I 252 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
FspI 2555 TGCGCA TGC^GCA
3356 TGCGCA
MroI 992 TCCGGA T^CCGGA
2453 TCCGGA
PmaCI 3120 CACGTG CAC^GTG
4342 CACGTG
PmlI 3120 CACGTG CAC^GTG
4342 CACGTG
SfiI 751 GGCCACTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2707 GGCCGCCAGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI SmlI Sse8387I StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI