################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AspI AvrII BbrPI BcgI BclI BfrI BglII BlnI BsaAI BseAI BsiWI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HpaI KspI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI Tth111I XcmI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 1591 AAATTT [GA]^AATT[CT] Aor51HI 114 AGCGCT AGC^GCT ApaI 259 GGGCCC GGGCC^C ApoI 1591 AAATTT [GA]^AATT[CT] BamHI 2235 GGATCC G^GATCC BbvCI 580 CCTCAGC CCTCAGC(-5/-2) BglI 1184 GCCTAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1673 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1673 GCTCAGC GC^T[ACGT]AGC BsaWI 2351 ACCGGA [AT]^CCGG[AT] Bsp1407I 609 TGTACA T^GTACA BspLU11I 1751 ACATGT A^CATGT BsrGI 609 TGTACA T^GTACA CelII 1673 GCTCAGC GC^T[ACGT]AGC DrdI 1021 GACATGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 114 AGCGCT AGC^GCT EcoNI 687 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1175 GATATC GAT^ATC HgaI 2443 GACGC GACGC(5/10) HindIII 148 AAGCTT A^AGCTT MseI 1316 TTAA T^TAA NaeI 585 GCCGGC GCC^GGC NcoI 1442 CCATGG C^CATGG NgoMIV 585 GCCGGC G^CCGGC NotI 99 GCGGCCGC GC^GGCCGC NspI 1751 ACATGT [GA]CATG^[CT] PaeR7I 1466 CTCGAG C^TCGAG SacI 289 GAGCTC GAGCT^C SapI 1657 GAAGAGC GCTCTTC(1/4) ScaI 1206 AGTACT AGT^ACT SexAI 1271 ACCTGGT A^CC[AT]GGT SmaI 15 CCCGGG CCC^GGG SpeI 1162 ACTAGT A^CTAGT SspBI 609 TGTACA T^GTACA TfiI 1238 GATTC G^A[AT]TC Tru9I 1316 TTAA T^TAA Tsp509I 1592 AATT ^AATT XbaI 1117 TCTAGA T^CTAGA XhoI 1466 CTCGAG C^TCGAG XmaCI 15 CCCGGG C^CCGGG XmaI 15 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 226 GGCGCC G[GA]^CG[CT]C 301 GGCGCC AflIII 770 ACGTGT A^C[GA][CT]GT 1751 ACATGT Asp700I 1326 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2331 GAACAACTTC AviII 311 TGCGCA TGC^GCA 2261 TGCGCA BalI 339 TGGCCA TGG^CCA 2549 TGGCCA BbeI 226 GGCGCC GGCGC^C 301 GGCGCC BbsI 1492 GTCTTC GAAGAC(2/6) 2457 GTCTTC BciVI 894 GGATAC GTATCC(6/5) 1680 GGATAC BpuAI 1492 GTCTTC GAAGAC(2/6) 2457 GTCTTC BsaBI 1050 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2403 GATCTACATC BsaHI 226 GGCGCC G[GA]^CG[CT]C 301 GGCGCC BsiEI 100 CGGCCG CG[GA][CT]^CG 517 CGGCCG BsmBI 1090 CGTCTC CGTCTC(1/5) 1972 GAGACG BsmI 1140 GAATGC GAATGC(1/-1) 1485 GAATGC BstXI 1617 CCAAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1722 CCAGGGCAATGG EagI 100 CGGCCG C^GGCCG 517 CGGCCG EarI 2479 CTCTTC CTCTTC(1/4) 1657 GAAGAG EclXI 100 CGGCCG C^GGCCG 517 CGGCCG Eco52I 100 CGGCCG C^GGCCG 517 CGGCCG FspI 311 TGCGCA TGC^GCA 2261 TGCGCA Hin1I 226 GGCGCC G[GA]^CG[CT]C 301 GGCGCC KasI 226 GGCGCC G^GCGCC 301 GGCGCC Ksp632I 2479 CTCTTC CTCTTC(1/4) 1657 GAAGAG MamI 1050 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2403 GATCTACATC MluNI 339 TGGCCA TGG^CCA 2549 TGGCCA MscI 339 TGGCCA TGG^CCA 2549 TGGCCA MslI 2064 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2559 CACTGACATG NarI 226 GGCGCC GG^CGCC 301 GGCGCC PflMI 1290 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2028 CCAACAGGTGG PstI 403 CTGCAG CTGCA^G 1231 CTGCAG SfoI 226 GGCGCC GGC^GCC 301 GGCGCC SmlI 1466 CTCGAG C^T[CT][GA]AG 1516 CTCAAG StuI 1359 AGGCCT AGG^CCT 1512 AGGCCT Van91I 1290 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2028 CCAACAGGTGG XmnI 1326 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2331 GAACAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI AlwI AlwNI Asp718I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII