(Circular) (Six-base) MAPSORT of: pFN21AE3315.seq Check: 1418 from: 1 to: 7288 pFN21AE3315 7288 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4135 4135 Size: 7288 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5098 278 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4631 1937 Size: 2694 4594 Acc65I G'GTAC_C Cuts at: 2252 2461 3280 4180 4609 2252 Size: 209 819 900 429 4931 Fragments arranged by size: 4931 900 819 429 209 AclI AA'CG_TT Cuts at: 1563 5415 5788 1563 Size: 3852 373 3063 Fragments arranged by size: 3852 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2984 3228 3295 4193 5351 6735 7003 1632 Size: 1352 244 67 898 1158 1384 268 1917 Fragments arranged by size: 1917 1384 1352 1158 898 268 244 67 AfeI AGC'GCT Cuts at: 2121 2121 Size: 7288 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7091 AflIII A'CryG_T Cuts at: 1203 2775 3756 6187 7164 1203 Size: 1572 981 2431 977 1327 Fragments arranged by size: 2431 1572 1327 981 977 AgeI A'CCGG_T Cuts at: 6119 6119 Size: 7288 AhdI GACnn_n'nnGTC Cuts at: 6017 6017 Size: 7288 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4412 1184 Size: 3228 4060 AlwNI CAG_nnn'CTG Cuts at: 2053 2612 3382 5031 6603 2053 Size: 559 770 1649 1572 2738 Fragments arranged by size: 2738 1649 1572 770 559 ApaI G_GGCC'C Cuts at: 2268 2268 Size: 7288 ApaLI G'TGCA_C Cuts at: 5345 6501 5345 Size: 1156 6132 ApoI r'AATT_y Cuts at: 1493 3596 4597 4823 4975 7084 1493 Size: 2103 1001 226 152 2109 1697 Fragments arranged by size: 2109 2103 1697 1001 226 152 AseI AT'TA_AT Cuts at: 160 1032 5842 160 Size: 872 4810 1606 Fragments arranged by size: 4810 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 AvaI C'yCGr_G Cuts at: 1870 1943 2020 3471 3750 4457 4613 1870 Size: 73 77 1451 279 707 156 4545 Fragments arranged by size: 4545 1451 707 279 156 77 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3678 3711 3678 Size: 33 7255 BamHI G'GATC_C Cuts at: 4240 4618 4985 4240 Size: 378 367 6543 Fragments arranged by size: 6543 378 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 2252 2306 2461 2867 Size: 325 217 627 444 21 54 155 406 Cuts at: 2867 3197 3280 3305 4180 4609 6064 618 Size: 330 83 25 875 429 1455 1842 Fragments arranged by size: 1842 1455 875 627 444 429 406 330 325 217 155 83 54 25 21 BanII G_rGCy'C Cuts at: 1391 2268 2298 2355 2455 4577 4607 1391 Size: 877 30 57 100 2122 30 4072 Fragments arranged by size: 4072 2122 877 100 57 30 30 BbeI G_GCGC'C Cuts at: 2235 2310 2235 Size: 75 7213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3490 4455 928 Size: 971 1591 965 3761 Fragments arranged by size: 3761 1591 971 965 BbvCI CC'TCA_GC Cuts at: 2586 2586 Size: 7288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5479 5513 1903 Size: 34 3542 34 3678 Fragments arranged by size: 3678 3542 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2893 3679 5180 6390 2893 Size: 786 1501 1210 3791 Fragments arranged by size: 3791 1501 1210 786 BclI T'GATC_A Cuts at: 1548 1548 Size: 7288 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3195 5899 136 Size: 107 122 71 631 605 1523 2704 1525 Fragments arranged by size: 2704 1525 1523 631 605 122 107 71 BglII A'GATC_T Cuts at: 7283 7283 Size: 7288 BlpI GC'TnA_GC Cuts at: 3679 4705 3679 Size: 1026 6262 Bme1580I G_kGCm'C Cuts at: 1790 2268 2870 3308 3674 3699 5349 6505 1790 Size: 478 602 438 366 25 1650 1156 2573 Fragments arranged by size: 2573 1650 1156 602 478 438 366 25 BmgBI CAC'GTC Cuts at: 1318 2980 1318 Size: 1662 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2821 2824 2851 Size: 416 53 83 399 24 1404 3 27 Cuts at: 2851 3311 5019 5977 442 Size: 460 1708 958 1753 Fragments arranged by size: 1753 1708 1404 958 460 416 399 83 53 27 24 3 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2162 3248 2162 Size: 1086 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2471 3041 3925 4459 5948 1239 Size: 815 417 570 884 534 1489 2579 Fragments arranged by size: 2579 1489 884 815 570 534 417 Bpu10I CC'TnA_GC Cuts at: 2531 2586 2531 Size: 55 7233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 5413 6278 6576 3506 Size: 1907 865 298 4218 Fragments arranged by size: 4218 1907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2575 2987 3468 4433 5951 882 Size: 617 1076 412 481 965 1518 2219 Fragments arranged by size: 2219 1518 1076 965 617 481 412 BsaAI yAC'GTr Cuts at: 493 1204 5079 493 Size: 711 3875 2702 Fragments arranged by size: 3875 2702 711 BsaBI GATnn'nnATC Cuts at: 3059 4412 7282 3059 Size: 1353 2870 3065 Fragments arranged by size: 3065 2870 1353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2307 5095 5477 Size: 53 83 186 936 699 75 2788 382 Cuts at: 5477 6112 275 Size: 635 1451 Fragments arranged by size: 2788 1451 936 699 635 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 4356 5721 6119 6393 6540 6951 4356 Size: 1365 398 274 147 411 4693 Fragments arranged by size: 4693 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2738 2768 1178 Size: 30 165 30 1335 30 5698 Fragments arranged by size: 5698 1335 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 2780 3908 4145 4535 4598 1186 Size: 1159 435 1128 237 390 63 3876 Fragments arranged by size: 3876 1159 1128 435 390 237 63 BseYI C'CCAG_C Cuts at: 2053 2167 2242 3407 3919 4429 6491 6987 2053 Size: 114 75 1165 512 510 2062 496 2354 Fragments arranged by size: 2354 2062 1165 512 510 496 114 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3254 4083 4334 1163 Size: 573 1518 829 251 4117 Fragments arranged by size: 4117 1518 829 573 251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2108 2525 4738 5499 6527 1540 Size: 275 186 107 417 2213 761 1028 2301 Fragments arranged by size: 2301 2213 1028 761 417 275 186 107 BsiHKAI G_wGCw'C Cuts at: 2148 2298 2391 2532 5349 5434 6505 2148 Size: 150 93 141 2817 85 1071 2931 Fragments arranged by size: 2931 2817 1071 150 141 93 85 BsmI GAATG_Cn' Cuts at: 3151 3496 4799 4892 3151 Size: 345 1303 93 5547 Fragments arranged by size: 5547 1303 345 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3101 3971 3101 Size: 870 6418 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2268 2298 2355 2391 2455 2532 Size: 399 358 120 30 57 36 64 77 Cuts at: 2532 2870 3308 3674 3699 4577 4607 5349 5434 Size: 338 438 366 25 878 30 742 85 Cuts at: 5434 6505 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 878 742 438 399 366 358 338 120 85 77 64 57 36 30 30 25 BspEI T'CCGG_A Cuts at: 6951 6951 Size: 7288 BspHI T'CATG_A Cuts at: 5177 6907 5177 Size: 1730 5558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3292 4135 4154 4643 844 Size: 2448 843 19 489 3489 Fragments arranged by size: 3489 2448 843 489 19 BsrBI CCG'CTC Cuts at: 1394 1823 5175 1394 Size: 429 3352 3507 Fragments arranged by size: 3507 3352 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2347 2803 3739 5783 5957 65 Size: 1183 1099 456 936 2044 174 1396 Fragments arranged by size: 2044 1396 1183 1099 936 456 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2194 2255 2524 2590 5932 6119 1700 Size: 110 384 61 269 66 3342 187 2869 Fragments arranged by size: 3342 2869 384 269 187 110 66 61 BsrGI T'GTAC_A Cuts at: 96 2614 96 Size: 2518 4770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7288 BssSI C'ACGA_G Cuts at: 5348 6360 5348 Size: 1012 6276 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7288 BstBI TT'CG_AA Cuts at: 6092 6092 Size: 7288 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7288 BstXI CCAn_nnnn'nTGG Cuts at: 3629 3734 3629 Size: 105 7183 BstYI r'GATC_y Cuts at: 1972 3054 3252 4240 4407 4618 4985 5370 5387 Size: 1082 198 988 167 211 367 385 17 Cuts at: 5387 6839 6925 6937 7283 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1082 988 385 367 346 211 198 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2322 2347 3447 513 Size: 552 939 318 25 1100 4354 Fragments arranged by size: 4354 1100 939 552 318 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2552 2835 487 Size: 683 56 1326 283 4940 Fragments arranged by size: 4940 1326 683 283 56 BtsI GCAGTG_nn' Cuts at: 4804 5598 5618 4804 Size: 794 20 6474 Fragments arranged by size: 6474 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7288 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2913 2948 529 Size: 35 2349 35 4869 Fragments arranged by size: 4869 2349 35 35 DraI TTT'AAA Cuts at: 4592 4592 Size: 7288 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3032 6295 809 Size: 507 1716 3263 1802 Fragments arranged by size: 3263 1802 1716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 2344 2522 Size: 54 1425 50 275 18 275 239 178 Cuts at: 2522 3359 4554 4735 5624 7253 8 Size: 837 1195 181 889 1629 43 Fragments arranged by size: 1629 1425 1195 889 837 275 275 239 181 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2105 2522 4735 1537 Size: 275 293 417 2213 4090 Fragments arranged by size: 4090 2213 417 293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3657 4490 5218 1353 Size: 2304 833 728 3423 Fragments arranged by size: 3423 2304 833 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 2463 5875 6249 6395 387 Size: 1064 138 867 7 3412 374 146 1280 Fragments arranged by size: 3412 1280 1064 867 374 146 138 7 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 7288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2471 2984 3041 3228 3295 3925 Size: 393 422 417 513 57 187 67 630 Cuts at: 3925 4193 4459 5351 5948 6735 7003 1239 Size: 268 266 892 597 787 268 1524 Fragments arranged by size: 1524 892 787 630 597 513 422 417 393 268 268 266 187 67 57 EcoNI CCTnn'n_nnAGG Cuts at: 2696 2696 Size: 7288 EcoO109I rG'GnC_Cy Cuts at: 2012 2164 2212 2265 2665 2826 2012 Size: 152 48 53 400 161 6474 Fragments arranged by size: 6474 400 161 152 53 48 EcoRI G'AATT_C Cuts at: 4597 4975 4597 Size: 378 6910 EcoRV GAT'ATC Cuts at: 3182 3182 Size: 7288 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3427 3427 Size: 7288 FspI TGC'GCA Cuts at: 2318 4268 5794 2318 Size: 1950 1526 3812 Fragments arranged by size: 3812 1950 1526 FspAI rTGC'GCAy Cuts at: 2318 2318 Size: 7288 HaeII r_GCGC'y Cuts at: 1678 2123 2235 2310 6435 1678 Size: 445 112 75 4125 2531 Fragments arranged by size: 4125 2531 445 112 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2738 2770 5929 5961 6003 Size: 32 135 32 1166 32 3159 32 42 Cuts at: 6003 6035 1373 Size: 32 2626 Fragments arranged by size: 3159 2626 1166 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4632 4878 669 Size: 988 281 2694 246 3079 Fragments arranged by size: 3079 2694 988 281 246 HindIII A'AGCT_T Cuts at: 748 2153 748 Size: 1405 5883 HpaI GTT'AAC Cuts at: 4878 4878 Size: 7288 KasI G'GCGC_C Cuts at: 2231 2306 2231 Size: 75 7213 KpnI G_GTAC'C Cuts at: 2256 2465 3284 4184 4613 2256 Size: 209 819 900 429 4931 Fragments arranged by size: 4931 900 819 429 209 MfeI C'AATT_G Cuts at: 4887 4887 Size: 7288 MluI A'CGCG_T Cuts at: 7164 7164 Size: 7288 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4129 4200 6402 6586 1305 Size: 2824 71 2202 184 2007 Fragments arranged by size: 2824 2202 2007 184 71 MscI TGG'CCA Cuts at: 10 64 1489 1832 2346 4556 10 Size: 54 1425 343 514 2210 2742 Fragments arranged by size: 2742 2210 1425 514 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4073 4568 5246 5605 5764 518 Size: 648 2907 495 678 359 159 2042 Fragments arranged by size: 2907 2042 678 648 495 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2110 2171 2380 2491 3130 3358 3683 Size: 444 60 61 209 111 639 228 325 Cuts at: 3683 3814 3923 4704 5381 6529 6774 1606 Size: 131 109 781 677 1148 245 2120 Fragments arranged by size: 2120 1148 781 677 639 444 325 245 228 209 131 111 109 61 60 NaeI GCC'GGC Cuts at: 2592 2592 Size: 7288 NarI GG'CG_CC Cuts at: 2232 2307 2232 Size: 75 7213 NcoI C'CATG_G Cuts at: 513 1065 2004 3447 513 Size: 552 939 1443 4354 Fragments arranged by size: 4354 1443 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7288 NgoMIV G'CCGG_C Cuts at: 2590 2590 Size: 7288 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7288 NotI GC'GGCC_GC Cuts at: 2105 4735 2105 Size: 2630 4658 NspI r_CATG'y Cuts at: 1132 1229 3760 4646 6191 1132 Size: 97 2531 886 1545 2229 Fragments arranged by size: 2531 2229 1545 886 97 PasI CC'CwG_GG Cuts at: 1348 4055 1348 Size: 2707 4581 PciI A'CATG_T Cuts at: 3756 6187 3756 Size: 2431 4857 PflMI CCAn_nnn'nTGG Cuts at: 1108 3301 4039 1108 Size: 2193 738 4357 Fragments arranged by size: 4357 2193 738 PfoI T'CCnGG_A Cuts at: 3018 3636 3948 3018 Size: 618 312 6358 Fragments arranged by size: 6358 618 312 PmeI GTTT'AAAC Cuts at: 4592 4592 Size: 7288 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5357 5389 6870 6902 5357 Size: 32 1481 32 5743 Fragments arranged by size: 5743 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2012 2164 2665 2826 2012 Size: 152 501 161 6474 Fragments arranged by size: 6474 501 161 152 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7288 PsiI TTA'TAA Cuts at: 4858 4858 Size: 7288 PspOMI G'GGCC_C Cuts at: 2264 2264 Size: 7288 PstI C_TGCA'G Cuts at: 830 2412 3240 4640 830 Size: 1582 828 1400 3478 Fragments arranged by size: 3478 1582 1400 828 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7288 PvuII CAG'CTG Cuts at: 2050 2380 3814 3923 2050 Size: 330 1434 109 5415 Fragments arranged by size: 5415 1434 330 109 SacI G_AGCT'C Cuts at: 2298 2298 Size: 7288 SalI G'TCGA_C Cuts at: 1936 4630 1936 Size: 2694 4594 SanDI GG'GwC_CC Cuts at: 2826 2826 Size: 7288 SapI GCTCTTCn'nnn_ Cuts at: 3657 3657 Size: 7288 SbfI CC_TGCA'GG Cuts at: 4640 4640 Size: 7288 ScaI AGT'ACT Cuts at: 3213 5536 7248 3213 Size: 2323 1712 3253 Fragments arranged by size: 3253 2323 1712 SexAI A'CCwGG_T Cuts at: 3276 3276 Size: 7288 SfcI C'TryA_G Cuts at: 826 1045 2408 3236 3556 4636 5771 6452 6643 Size: 219 1363 828 320 1080 1135 681 191 Cuts at: 6643 826 Size: 1471 Fragments arranged by size: 1471 1363 1135 1080 828 681 320 219 191 SfoI GGC'GCC Cuts at: 2233 2308 2233 Size: 75 7213 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7288 SmaI CCC'GGG Cuts at: 1872 2022 4615 1872 Size: 150 2593 4545 Fragments arranged by size: 4545 2593 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 3471 3521 5392 6293 6555 820 Size: 197 926 1528 50 1871 901 262 1553 Fragments arranged by size: 1871 1553 1528 926 901 262 197 50 SnaBI TAC'GTA Cuts at: 493 5079 493 Size: 4586 2702 SpeI A'CTAG_T Cuts at: 152 3167 152 Size: 3015 4273 SphI G_CATG'C Cuts at: 1132 4646 1132 Size: 3514 3774 SrfI GCCC'GGGC Cuts at: 2022 2022 Size: 7288 SspI AAT'ATT Cuts at: 5 52 5212 5 Size: 47 5160 2081 Fragments arranged by size: 5160 2081 47 StuI AGG'CCT Cuts at: 1517 3366 3519 1517 Size: 1849 153 5286 Fragments arranged by size: 5286 1849 153 StyI C'CwwG_G Cuts at: 513 1065 2004 3447 3622 4509 4577 4727 513 Size: 552 939 1443 175 887 68 150 3074 Fragments arranged by size: 3074 1443 939 887 552 175 150 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2347 3518 5315 5332 5485 5670 6115 Size: 1255 767 1171 1797 17 153 185 445 Cuts at: 6115 325 Size: 1498 Fragments arranged by size: 1797 1498 1255 1171 767 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2614 3211 4021 Size: 274 80 33 51 1443 637 597 810 Cuts at: 4021 5534 7246 96 Size: 1513 1712 138 Fragments arranged by size: 1712 1513 1443 810 637 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3545 3577 7248 7280 3545 Size: 32 3671 32 3553 Fragments arranged by size: 3671 3553 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7288 XbaI T'CTAG_A Cuts at: 3122 4624 3122 Size: 1502 5786 XhoI C'TCGA_G Cuts at: 1943 3471 1943 Size: 1528 5760 XmaI C'CCGG_G Cuts at: 1870 2020 4613 1870 Size: 150 2593 4545 Fragments arranged by size: 4545 2593 150 XmnI GAAnn'nnTTC Cuts at: 3335 4340 5417 3335 Size: 1005 1077 5206 Fragments arranged by size: 5206 1077 1005 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5096 276 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SwaI XcmI