(Linear) (Six-base) MAPSORT of: KIEE3315.seq Check: 9033 from: 1 to: 2583 KIEE3315.orf With 182 enzymes: SgfI * July 3, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2130 2583 Size: 2130 453 Acc65I G'GTAC_C Cuts at: 0 247 456 1275 2175 2583 Size: 247 209 819 900 408 Fragments arranged by size: 900 819 408 247 209 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1223 1290 2188 2583 Size: 979 244 67 898 395 Fragments arranged by size: 979 898 395 244 67 AfeI AGC'GCT Cuts at: 0 116 2583 Size: 116 2467 AflIII A'CryG_T Cuts at: 0 770 1751 2583 Size: 770 981 832 Fragments arranged by size: 981 832 770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2407 2583 Size: 2407 176 AlwNI CAG_nnn'CTG Cuts at: 0 48 607 1377 2583 Size: 48 559 770 1206 Fragments arranged by size: 1206 770 559 48 ApaI G_GGCC'C Cuts at: 0 263 2583 Size: 263 2320 ApoI r'AATT_y Cuts at: 0 1591 2583 Size: 1591 992 AvaI C'yCGr_G Cuts at: 0 15 1466 1745 2452 2583 Size: 15 1451 279 707 131 Fragments arranged by size: 1451 707 279 131 15 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1673 1706 2583 Size: 1673 33 877 Fragments arranged by size: 1673 877 33 BamHI G'GATC_C Cuts at: 0 2235 2583 Size: 2235 348 BanI G'GyrC_C Cuts at: 0 226 247 301 456 862 1192 1275 1300 Size: 226 21 54 155 406 330 83 25 Cuts at: 1300 2175 2583 Size: 875 408 Fragments arranged by size: 875 408 406 330 226 155 83 54 25 21 BanII G_rGCy'C Cuts at: 0 263 293 350 450 2572 2583 Size: 263 30 57 100 2122 11 Fragments arranged by size: 2122 263 100 57 30 11 BbeI G_GCGC'C Cuts at: 0 230 305 2583 Size: 230 75 2278 Fragments arranged by size: 2278 230 75 BbsI GAAGACnn'nnnn_ Cuts at: 0 1485 2450 2583 Size: 1485 965 133 Fragments arranged by size: 1485 965 133 BbvCI CC'TCA_GC Cuts at: 0 581 2583 Size: 581 2002 BciVI GTATCCnnnnn_n' Cuts at: 0 888 1674 2583 Size: 888 786 909 Fragments arranged by size: 909 888 786 BglI GCCn_nnn'nGGC Cuts at: 0 1190 2583 Size: 1190 1393 BlpI GC'TnA_GC Cuts at: 0 1674 2583 Size: 1674 909 Bme1580I G_kGCm'C Cuts at: 0 263 865 1303 1669 1694 2583 Size: 263 602 438 366 25 889 Fragments arranged by size: 889 602 438 366 263 25 BmgBI CAC'GTC Cuts at: 0 975 2583 Size: 975 1608 BmrI ACTGGGnnnn_n' Cuts at: 0 816 819 846 1306 2583 Size: 816 3 27 460 1277 Fragments arranged by size: 1277 816 460 27 3 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 157 1243 2583 Size: 157 1086 1340 Fragments arranged by size: 1340 1086 157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 466 1036 1920 2454 2583 Size: 49 417 570 884 534 129 Fragments arranged by size: 884 570 534 417 129 49 Bpu10I CC'TnA_GC Cuts at: 0 526 581 2583 Size: 526 55 2002 Fragments arranged by size: 2002 526 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1501 2583 Size: 1501 1082 BsaI GGTCTCn'nnnn_ Cuts at: 0 570 982 1463 2428 2583 Size: 570 412 481 965 155 Fragments arranged by size: 965 570 481 412 155 BsaBI GATnn'nnATC Cuts at: 0 1054 2407 2583 Size: 1054 1353 176 Fragments arranged by size: 1353 1054 176 BsaHI Gr'CG_yC Cuts at: 0 227 302 2583 Size: 227 75 2281 Fragments arranged by size: 2281 227 75 BsaWI w'CCGG_w Cuts at: 0 2351 2583 Size: 2351 232 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 733 763 2583 Size: 733 30 1820 Fragments arranged by size: 1820 733 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 340 775 1903 2140 2530 2583 Size: 340 435 1128 237 390 53 Fragments arranged by size: 1128 435 390 340 237 53 BseYI C'CCAG_C Cuts at: 0 48 162 237 1402 1914 2424 2583 Size: 48 114 75 1165 512 510 159 Fragments arranged by size: 1165 512 510 159 114 75 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1249 2078 2329 2583 Size: 1249 829 251 254 Fragments arranged by size: 1249 829 254 251 BsiEI CG_ry'CG Cuts at: 0 103 520 2583 Size: 103 417 2063 Fragments arranged by size: 2063 417 103 BsiHKAI G_wGCw'C Cuts at: 0 143 293 386 527 2583 Size: 143 150 93 141 2056 Fragments arranged by size: 2056 150 143 141 93 BsmI GAATG_Cn' Cuts at: 0 1146 1491 2583 Size: 1146 345 1092 Fragments arranged by size: 1146 1092 345 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1096 1966 2583 Size: 1096 870 617 Fragments arranged by size: 1096 870 617 Bsp1286I G_dGCh'C Cuts at: 0 143 263 293 350 386 450 527 865 Size: 143 120 30 57 36 64 77 338 Cuts at: 865 1303 1669 1694 2572 2583 Size: 438 366 25 878 11 Fragments arranged by size: 878 438 366 338 143 120 77 64 57 36 30 25 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1287 2130 2149 2583 Size: 1287 843 19 434 Fragments arranged by size: 1287 843 434 19 BsrDI GCAATG_nn' Cuts at: 0 342 798 1734 2583 Size: 342 456 936 849 Fragments arranged by size: 936 849 456 342 BsrFI r'CCGG_y Cuts at: 0 189 250 519 585 2583 Size: 189 61 269 66 1998 Fragments arranged by size: 1998 269 189 66 61 BsrGI T'GTAC_A Cuts at: 0 609 2583 Size: 609 1974 BstXI CCAn_nnnn'nTGG Cuts at: 0 1624 1729 2583 Size: 1624 105 854 Fragments arranged by size: 1624 854 105 BstYI r'GATC_y Cuts at: 0 1049 1247 2235 2402 2583 Size: 1049 198 988 167 181 Fragments arranged by size: 1049 988 198 181 167 BtgI C'CryG_G Cuts at: 0 317 342 1442 2583 Size: 317 25 1100 1141 Fragments arranged by size: 1141 1100 317 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 547 830 2583 Size: 547 283 1753 Fragments arranged by size: 1753 547 283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 908 943 2583 Size: 908 35 1640 Fragments arranged by size: 1640 908 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1027 2583 Size: 1027 1556 EaeI y'GGCC_r Cuts at: 0 100 339 517 1354 2549 2583 Size: 100 239 178 837 1195 34 Fragments arranged by size: 1195 837 239 178 100 34 EagI C'GGCC_G Cuts at: 0 100 517 2583 Size: 100 417 2066 Fragments arranged by size: 2066 417 100 EarI CTCTTCn'nnn_ Cuts at: 0 1652 2485 2583 Size: 1652 833 98 Fragments arranged by size: 1652 833 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 458 2583 Size: 451 7 2125 Fragments arranged by size: 2125 451 7 EcoICRI GAG'CTC Cuts at: 0 291 2583 Size: 291 2292 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 466 979 1036 1223 1290 1920 2188 Size: 49 417 513 57 187 67 630 268 Cuts at: 2188 2454 2583 Size: 266 129 Fragments arranged by size: 630 513 417 268 266 187 129 67 57 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 691 2583 Size: 691 1892 EcoO109I rG'GnC_Cy Cuts at: 0 7 159 207 260 660 821 2583 Size: 7 152 48 53 400 161 1762 Fragments arranged by size: 1762 400 161 152 53 48 7 EcoRV GAT'ATC Cuts at: 0 1177 2583 Size: 1177 1406 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1422 2583 Size: 1422 1161 FspI TGC'GCA Cuts at: 0 313 2263 2583 Size: 313 1950 320 Fragments arranged by size: 1950 320 313 FspAI rTGC'GCAy Cuts at: 0 313 2583 Size: 313 2270 HaeII r_GCGC'y Cuts at: 0 118 230 305 2583 Size: 118 112 75 2278 Fragments arranged by size: 2278 118 112 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 733 765 2583 Size: 733 32 1818 Fragments arranged by size: 1818 733 32 HindIII A'AGCT_T Cuts at: 0 148 2583 Size: 148 2435 KasI G'GCGC_C Cuts at: 0 226 301 2583 Size: 226 75 2282 Fragments arranged by size: 2282 226 75 KpnI G_GTAC'C Cuts at: 0 251 460 1279 2179 2583 Size: 251 209 819 900 404 Fragments arranged by size: 900 819 404 251 209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2124 2195 2583 Size: 2124 71 388 Fragments arranged by size: 2124 388 71 MscI TGG'CCA Cuts at: 0 341 2551 2583 Size: 341 2210 32 Fragments arranged by size: 2210 341 32 MslI CAynn'nnrTG Cuts at: 0 2068 2563 2583 Size: 2068 495 20 Fragments arranged by size: 2068 495 20 MspA1I CmG'CkG Cuts at: 0 45 105 166 375 486 1125 1353 1678 Size: 45 60 61 209 111 639 228 325 Cuts at: 1678 1809 1918 2583 Size: 131 109 665 Fragments arranged by size: 665 639 325 228 209 131 111 109 61 60 45 NaeI GCC'GGC Cuts at: 0 587 2583 Size: 587 1996 NarI GG'CG_CC Cuts at: 0 227 302 2583 Size: 227 75 2281 Fragments arranged by size: 2281 227 75 NcoI C'CATG_G Cuts at: 0 1442 2583 Size: 1442 1141 NgoMIV G'CCGG_C Cuts at: 0 585 2583 Size: 585 1998 NotI GC'GGCC_GC Cuts at: 0 100 2583 Size: 100 2483 NspI r_CATG'y Cuts at: 0 1755 2583 Size: 1755 828 PasI CC'CwG_GG Cuts at: 0 2050 2583 Size: 2050 533 PciI A'CATG_T Cuts at: 0 1751 2583 Size: 1751 832 PflMI CCAn_nnn'nTGG Cuts at: 0 1296 2034 2583 Size: 1296 738 549 Fragments arranged by size: 1296 738 549 PfoI T'CCnGG_A Cuts at: 0 1013 1631 1943 2583 Size: 1013 618 312 640 Fragments arranged by size: 1013 640 618 312 PpuMI rG'GwC_Cy Cuts at: 0 7 159 660 821 2583 Size: 7 152 501 161 1762 Fragments arranged by size: 1762 501 161 152 7 PspOMI G'GGCC_C Cuts at: 0 259 2583 Size: 259 2324 PstI C_TGCA'G Cuts at: 0 407 1235 2583 Size: 407 828 1348 Fragments arranged by size: 1348 828 407 PvuII CAG'CTG Cuts at: 0 45 375 1809 1918 2583 Size: 45 330 1434 109 665 Fragments arranged by size: 1434 665 330 109 45 SacI G_AGCT'C Cuts at: 0 293 2583 Size: 293 2290 SanDI GG'GwC_CC Cuts at: 0 821 2583 Size: 821 1762 SapI GCTCTTCn'nnn_ Cuts at: 0 1652 2583 Size: 1652 931 ScaI AGT'ACT Cuts at: 0 1208 2583 Size: 1208 1375 SexAI A'CCwGG_T Cuts at: 0 1271 2583 Size: 1271 1312 SfcI C'TryA_G Cuts at: 0 403 1231 1551 2583 Size: 403 828 320 1032 Fragments arranged by size: 1032 828 403 320 SfoI GGC'GCC Cuts at: 0 228 303 2583 Size: 228 75 2280 Fragments arranged by size: 2280 228 75 SmaI CCC'GGG Cuts at: 0 17 2583 Size: 17 2566 SmlI C'TyrA_G Cuts at: 0 1466 1516 2583 Size: 1466 50 1067 Fragments arranged by size: 1466 1067 50 SpeI A'CTAG_T Cuts at: 0 1162 2583 Size: 1162 1421 SrfI GCCC'GGGC Cuts at: 0 17 2583 Size: 17 2566 StuI AGG'CCT Cuts at: 0 1361 1514 2583 Size: 1361 153 1069 Fragments arranged by size: 1361 1069 153 StyI C'CwwG_G Cuts at: 0 1442 1617 2504 2572 2583 Size: 1442 175 887 68 11 Fragments arranged by size: 1442 887 175 68 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 342 1513 2583 Size: 342 1171 1070 Fragments arranged by size: 1171 1070 342 TatI w'GTAC_w Cuts at: 0 609 1206 2016 2583 Size: 609 597 810 567 Fragments arranged by size: 810 609 597 567 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1540 1572 2583 Size: 1540 32 1011 Fragments arranged by size: 1540 1011 32 XbaI T'CTAG_A Cuts at: 0 1117 2583 Size: 1117 1466 XhoI C'TCGA_G Cuts at: 0 1466 2583 Size: 1466 1117 XmaI C'CCGG_G Cuts at: 0 15 2583 Size: 15 2568 XmnI GAAnn'nnTTC Cuts at: 0 1330 2335 2583 Size: 1330 1005 248 Fragments arranged by size: 1330 1005 248 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SrfI StuI StyI TaqII TatI TstI XbaI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BcgI BclI BfrBI BglII BmtI BsaAI BsiWI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII EcoRI FseI HincII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SnaBI SphI SspI SwaI Tth111I XcmI ZraI