################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BcnI BfrI BlnI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I FauI FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1031 GTCTAC GT^[AC][GT]AC AlwNI 73 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 111 GGGCCC GGGCC^C AspHI 1749 GAGCAC G[AT]GC[AT]^C BanI 62 GGCACC G^G[CT][GA]CC BanII 111 GGGCCC G[GA]GC[CT]^C BbvCI 129 GCTGAGG CCTCAGC(-5/-2) BciVI 335 GGATAC GTATCC(6/5) BglI 113 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 54 GCTCAGC GC^T[ACGT]AGC BpmI 1016 CTCCAG CTGGAG(16/14) Bpu1102I 54 GCTCAGC GC^T[ACGT]AGC BsaBI 1726 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 588 GTGCAG GTGCAG(16/14) BsiHKAI 1749 GAGCAC G[AT]GC[AT]^C BsmFI 1571 GTCCC GGGAC(10/14) BsmI 751 GAATGC GAATGC(1/-1) BspLU11I 384 ACATGT A^CATGT BspMI 491 GCAGGT ACCTGC(4/8) BsrDI 1579 GCAATG GCAATG(2/0) BstAPI 1136 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 1628 GGATG GGATG(2/0) BstXI 1574 CCACTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 54 GCTCAGC GC^T[ACGT]AGC CfoI 76 GCGC GCG^C DraII 111 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO109I 111 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 628 GAATTC G^AATTC EcoRV 433 GATATC GAT^ATC FokI 1628 GGATG GGATG(9/13) HaeII 75 GGCGCT [GA]GCGC^[CT] HhaI 76 GCGC GCG^C HinP1I 76 GCGC G^CGC HincII 1061 GTCAAC GT[CT]^[GA]AC HindII 1061 GTCAAC GT[CT]^[GA]AC HphI 482 TCACC GGTGA(8/7) MamI 1726 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 57 CAGCTG C[AC]G^C[GT]G NspI 384 ACATGT [GA]CATG^[CT] PleI 1671 GACTC GAGTC(4/5) PstI 656 CTGCAG CTGCA^G PvuII 57 CAGCTG CAG^CTG ScaI 1861 AGTACT AGT^ACT SfcI 656 CTGCAG C^T[GA][CT]AG SmlI 42 CTTGAG C^T[CT][GA]AG SspI 1561 AATATT AAT^ATT StuI 20 AGGCCT AGG^CCT TaqI 342 TCGA T^CGA TthHB8I 342 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 384 ACATGT A^C[GA][CT]GT 809 ACACGT Asp700I 252 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1885 GAAGGAGTTC BbsI 35 GAAGAC GAAGAC(2/6) 1668 GAAGAC BglII 533 AGATCT A^GATCT 1266 AGATCT BmyI 111 GGGCCC G[AGT]GC[ACT]^C 1749 GAGCAC BpuAI 35 GAAGAC GAAGAC(2/6) 1668 GAAGAC BseRI 136 GAGGAG GAGGAG(10/8) 139 GAGGAG Bsp1286I 111 GGGCCC G[AGT]GC[ACT]^C 1749 GAGCAC BstYI 533 AGATCT [GA]^GATC[CT] 1266 AGATCT Cac8I 59 GCTGGC GC[ACGT]^[ACGT]GC 1011 GCTTGC Cfr13I 111 GGGCC G^G[ACGT]CC 112 GGCCC EcoNI 23 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 485 CCTTTTGCAGG EcoT14I 268 CCAAGG C^C[AT][AT]GG 687 CCTTGG HapII 145 CCGG C^CGG 1626 CCGG HindIII 262 AAGCTT A^AGCTT 472 AAGCTT HpaII 145 CCGG C^CGG 1626 CCGG MaeII 185 ACGT A^CGT 811 ACGT MflI 533 AGATCT [GA]^GATC[CT] 1266 AGATCT MspI 145 CCGG C^CGG 1626 CCGG Sau96I 111 GGGCC G^G[ACGT]CC 112 GGCCC SpeI 648 ACTAGT A^CTAGT 1615 ACTAGT StyI 268 CCAAGG C^C[AT][AT]GG 687 CCTTGG XhoII 533 AGATCT [GA]^GATC[CT] 1266 AGATCT XmnI 252 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1885 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI DdeI DpnII EcoRII EcoT22I Fnu4HI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MfeI MnlI MseI MslI MunI MvaI MwoI NdeII NlaIII NlaIV NsiI RsaI Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI