(Circular) (Six-base) MAPSORT of: pFN21AE4172.seq Check: 4225 from: 1 to: 6604 pFN21AE4172 6604 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4414 278 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3037 3947 1937 Size: 1100 910 4594 Fragments arranged by size: 4594 1100 910 Acc65I G'GTAC_C Cuts at: 3925 3925 Size: 6604 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4667 6051 6319 1632 Size: 3035 1384 268 1917 Fragments arranged by size: 3035 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 2389 2814 5503 6480 1203 Size: 1186 425 2689 977 1327 Fragments arranged by size: 2689 1327 1186 977 425 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6604 AlwNI CAG_nnn'CTG Cuts at: 2083 4347 5919 2083 Size: 2264 1572 2768 Fragments arranged by size: 2768 2264 1572 ApaI G_GGCC'C Cuts at: 2120 2120 Size: 6604 ApaLI G'TGCA_C Cuts at: 4661 5817 4661 Size: 1156 5448 ApoI r'AATT_y Cuts at: 1493 2261 2633 3193 3321 3439 3611 3913 4139 Size: 768 372 560 128 118 172 302 226 Cuts at: 4139 4291 6400 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 768 560 372 302 226 172 152 128 118 AseI AT'TA_AT Cuts at: 160 1032 5158 160 Size: 872 4126 1606 Fragments arranged by size: 4126 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 3929 1870 Size: 73 1986 4545 Fragments arranged by size: 4545 1986 73 BamHI G'GATC_C Cuts at: 3934 4301 3934 Size: 367 6237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 3925 5380 618 Size: 325 217 627 280 1858 1455 1842 Fragments arranged by size: 1858 1842 1455 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 2120 3923 1391 Size: 729 1803 4072 Fragments arranged by size: 4072 1803 729 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2047 3680 928 Size: 971 148 1633 3852 Fragments arranged by size: 3852 1633 971 148 BbvCI CC'TCA_GC Cuts at: 2135 2135 Size: 6604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4795 4829 1903 Size: 34 50 34 2774 34 3678 Fragments arranged by size: 3678 2774 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2334 4496 5706 2334 Size: 2162 1210 3232 Fragments arranged by size: 3232 2162 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6604 BfrBI ATG'CAT Cuts at: 2532 2760 3600 2532 Size: 228 840 5536 Fragments arranged by size: 5536 840 228 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 5215 136 Size: 107 122 71 631 605 452 3091 1525 Fragments arranged by size: 3091 1525 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 2538 3271 6599 2538 Size: 733 3328 2543 Fragments arranged by size: 3328 2543 733 BlpI GC'TnA_GC Cuts at: 2060 4021 2060 Size: 1961 4643 Bme1580I G_kGCm'C Cuts at: 1790 2120 4665 5821 1790 Size: 330 2545 1156 2573 Fragments arranged by size: 2573 2545 1156 330 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 4335 5293 Size: 416 53 83 399 24 892 2026 958 Cuts at: 5293 442 Size: 1753 Fragments arranged by size: 2026 1753 958 892 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3006 5264 1239 Size: 1767 2258 2579 Fragments arranged by size: 2579 2258 1767 Bpu10I CC'TnA_GC Cuts at: 2135 2135 Size: 6604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2068 4729 5594 5892 2068 Size: 2661 865 298 2780 Fragments arranged by size: 2780 2661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5267 882 Size: 617 3768 2219 Fragments arranged by size: 3768 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4395 493 Size: 711 3191 2702 Fragments arranged by size: 3191 2702 711 BsaBI GATnn'nnATC Cuts at: 3735 6598 3735 Size: 2863 3741 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4411 4793 5428 275 Size: 53 83 186 936 2878 382 635 1451 Fragments arranged by size: 2878 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5037 5435 5709 5856 6267 5037 Size: 398 274 147 411 5374 Fragments arranged by size: 5374 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2970 3000 1178 Size: 30 165 30 1567 30 4782 Fragments arranged by size: 4782 1567 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2159 1186 Size: 970 3 5631 Fragments arranged by size: 5631 970 3 BseYI C'CCAG_C Cuts at: 2244 5807 6303 2244 Size: 3563 496 2545 Fragments arranged by size: 3563 2545 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2614 1163 Size: 573 878 5153 Fragments arranged by size: 5153 878 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4815 5843 1540 Size: 275 186 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3758 4665 4750 5821 3758 Size: 907 85 1071 4541 Fragments arranged by size: 4541 1071 907 85 BsmI GAATG_Cn' Cuts at: 2762 4115 4208 2762 Size: 1353 93 5158 Fragments arranged by size: 5158 1353 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 3758 3923 4665 4750 5821 1391 Size: 399 330 1638 165 742 85 1071 2174 Fragments arranged by size: 2174 1638 1071 742 399 330 165 85 BspEI T'CCGG_A Cuts at: 6267 6267 Size: 6604 BspHI T'CATG_A Cuts at: 4493 6223 4493 Size: 1730 4874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 3959 844 Size: 1643 1472 3489 Fragments arranged by size: 3489 1643 1472 BsrBI CCG'CTC Cuts at: 1394 1823 4491 1394 Size: 429 2668 3507 Fragments arranged by size: 3507 2668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3591 5099 5273 65 Size: 1183 2343 1508 174 1396 Fragments arranged by size: 2343 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5248 5435 1700 Size: 110 3438 187 2869 Fragments arranged by size: 3438 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 3147 794 Size: 2353 4251 BstBI TT'CG_AA Cuts at: 5408 5408 Size: 6604 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6604 BstXI CCAn_nnnn'nTGG Cuts at: 3586 3586 Size: 6604 BstYI r'GATC_y Cuts at: 1972 2538 3271 3934 4301 4686 4703 6155 6241 Size: 566 733 663 367 385 17 1452 86 Cuts at: 6241 6253 6599 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 733 663 566 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6052 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5865 Fragments arranged by size: 5865 683 56 BtsI GCAGTG_nn' Cuts at: 2978 3207 3539 3579 4120 4914 4934 2978 Size: 229 332 40 541 794 20 4648 Fragments arranged by size: 4648 794 541 332 229 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6569 DraI TTT'AAA Cuts at: 3908 3908 Size: 6604 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5611 809 Size: 507 4295 1802 Fragments arranged by size: 4295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569 Size: 54 1425 50 275 18 2221 889 1629 Cuts at: 6569 8 Size: 43 Fragments arranged by size: 2221 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 1537 Size: 275 2239 4090 Fragments arranged by size: 4090 2239 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4534 1353 Size: 3181 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5191 5565 5711 387 Size: 1064 138 3602 374 146 1280 Fragments arranged by size: 3602 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3006 4667 5264 6051 6319 1239 Size: 393 1374 1661 597 787 268 1524 Fragments arranged by size: 1661 1524 1374 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2032 2494 2032 Size: 462 6142 EcoO109I rG'GnC_Cy Cuts at: 2117 2117 Size: 6604 EcoRI G'AATT_C Cuts at: 2633 3913 4291 2633 Size: 1280 378 4946 Fragments arranged by size: 4946 1280 378 EcoRV GAT'ATC Cuts at: 2440 2440 Size: 6604 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 6604 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 2084 5751 1678 Size: 406 3667 2531 Fragments arranged by size: 3667 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 5245 5277 5319 Size: 32 135 32 857 32 2784 32 42 Cuts at: 5319 5351 1373 Size: 32 2626 Fragments arranged by size: 2784 2626 857 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3068 3948 4194 669 Size: 988 281 1130 880 246 3079 Fragments arranged by size: 3079 1130 988 880 281 246 HindIII A'AGCT_T Cuts at: 748 2267 2477 748 Size: 1519 210 4875 Fragments arranged by size: 4875 1519 210 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3929 3929 Size: 6604 MfeI C'AATT_G Cuts at: 2727 3748 3899 4203 2727 Size: 1021 151 304 5128 Fragments arranged by size: 5128 1021 304 151 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3013 5718 5902 1305 Size: 1708 2705 184 2007 Fragments arranged by size: 2705 2007 1708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4782 Fragments arranged by size: 4782 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2573 3584 3814 4562 4921 5080 518 Size: 648 1407 1011 230 748 359 159 2042 Fragments arranged by size: 2042 1407 1011 748 648 359 230 159 MspA1I CmG'CkG Cuts at: 1606 2064 4020 4697 5845 6090 1606 Size: 458 1956 677 1148 245 2120 Fragments arranged by size: 2120 1956 1148 677 458 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6052 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NsiI A_TGCA'T Cuts at: 2534 2762 3602 2534 Size: 228 840 5536 Fragments arranged by size: 5536 840 228 NspI r_CATG'y Cuts at: 1132 1229 2393 3962 5507 1132 Size: 97 1164 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 1164 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6604 PciI A'CATG_T Cuts at: 2389 5503 2389 Size: 3114 3490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6604 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 2745 3465 4174 2745 Size: 720 709 5175 Fragments arranged by size: 5175 720 709 PspOMI G'GGCC_C Cuts at: 2116 2116 Size: 6604 PstI C_TGCA'G Cuts at: 830 2665 3956 830 Size: 1835 1291 3478 Fragments arranged by size: 3478 1835 1291 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 2064 2064 Size: 6604 SalI G'TCGA_C Cuts at: 1936 3946 1936 Size: 2010 4594 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 3868 4852 6564 3868 Size: 984 1712 3908 Fragments arranged by size: 3908 1712 984 SfcI C'TryA_G Cuts at: 826 1045 2661 3952 5087 5768 5959 826 Size: 219 1616 1291 1135 681 191 1471 Fragments arranged by size: 1616 1471 1291 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3931 1872 Size: 2059 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 4708 5609 5871 820 Size: 197 926 104 2661 901 262 1553 Fragments arranged by size: 2661 1553 926 901 262 197 104 SnaBI TAC'GTA Cuts at: 493 4395 493 Size: 3902 2702 SpeI A'CTAG_T Cuts at: 152 2653 3620 152 Size: 2501 967 3136 Fragments arranged by size: 3136 2501 967 SphI G_CATG'C Cuts at: 1132 3962 1132 Size: 2830 3774 SspI AAT'ATT Cuts at: 5 52 3568 4528 5 Size: 47 3516 960 2081 Fragments arranged by size: 3516 2081 960 47 StuI AGG'CCT Cuts at: 1517 2027 1517 Size: 510 6094 StyI C'CwwG_G Cuts at: 513 1065 2273 2692 4043 513 Size: 552 1208 419 1351 3074 Fragments arranged by size: 3074 1351 1208 552 419 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4631 4648 4801 4986 5431 325 Size: 1255 3051 17 153 185 445 1498 Fragments arranged by size: 3051 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2558 3089 3866 Size: 274 80 33 51 1443 581 531 777 Cuts at: 3866 4850 6562 96 Size: 984 1712 138 Fragments arranged by size: 1712 1443 984 777 581 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2970 3002 6564 6596 2970 Size: 32 3562 32 2978 Fragments arranged by size: 3562 2978 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6604 XbaI T'CTAG_A Cuts at: 3940 3940 Size: 6604 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6604 XmaI C'CCGG_G Cuts at: 1870 3929 1870 Size: 2059 4545 XmnI GAAnn'nnTTC Cuts at: 2261 3894 4733 2261 Size: 1633 839 4132 Fragments arranged by size: 4132 1633 839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4412 276 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI