(Linear) (Six-base) MAPSORT of: KIEE4172.seq Check: 7661 from: 1 to: 1899 KIEE4172.orf With 182 enzymes: SgfI * July 3, 2012 18:43 .. AccI GT'mk_AC Cuts at: 0 1032 1899 Size: 1032 867 AflIII A'CryG_T Cuts at: 0 384 809 1899 Size: 384 425 1090 Fragments arranged by size: 1090 425 384 AlwNI CAG_nnn'CTG Cuts at: 0 78 1899 Size: 78 1821 ApaI G_GGCC'C Cuts at: 0 115 1899 Size: 115 1784 ApoI r'AATT_y Cuts at: 0 256 628 1188 1316 1434 1606 1899 Size: 256 372 560 128 118 172 293 Fragments arranged by size: 560 372 293 256 172 128 118 BanI G'GyrC_C Cuts at: 0 62 1899 Size: 62 1837 BanII G_rGCy'C Cuts at: 0 115 1899 Size: 115 1784 BbsI GAAGACnn'nnnn_ Cuts at: 0 42 1675 1899 Size: 42 1633 224 Fragments arranged by size: 1633 224 42 BbvCI CC'TCA_GC Cuts at: 0 130 1899 Size: 130 1769 BciVI GTATCCnnnnn_n' Cuts at: 0 329 1899 Size: 329 1570 BfrBI ATG'CAT Cuts at: 0 527 755 1595 1899 Size: 527 228 840 304 Fragments arranged by size: 840 527 304 228 BglI GCCn_nnn'nGGC Cuts at: 0 119 1899 Size: 119 1780 BglII A'GATC_T Cuts at: 0 533 1266 1899 Size: 533 733 633 Fragments arranged by size: 733 633 533 BlpI GC'TnA_GC Cuts at: 0 55 1899 Size: 55 1844 Bme1580I G_kGCm'C Cuts at: 0 115 1899 Size: 115 1784 BmrI ACTGGGnnnn_n' Cuts at: 0 304 1899 Size: 304 1595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1899 Size: 1001 898 Bpu10I CC'TnA_GC Cuts at: 0 130 1899 Size: 130 1769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 1899 Size: 63 1836 BsaBI GATnn'nnATC Cuts at: 0 1730 1899 Size: 1730 169 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 965 995 1899 Size: 965 30 904 Fragments arranged by size: 965 904 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 154 1899 Size: 151 3 1745 Fragments arranged by size: 1745 151 3 BseYI C'CCAG_C Cuts at: 0 239 1899 Size: 239 1660 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 1899 Size: 609 1290 BsiHKAI G_wGCw'C Cuts at: 0 1753 1899 Size: 1753 146 BsmI GAATG_Cn' Cuts at: 0 757 1899 Size: 757 1142 Bsp1286I G_dGCh'C Cuts at: 0 115 1753 1899 Size: 115 1638 146 Fragments arranged by size: 1638 146 115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 482 1899 Size: 482 1417 BsrDI GCAATG_nn' Cuts at: 0 1586 1899 Size: 1586 313 BstAPI GCAn_nnn'nTGC Cuts at: 0 1142 1899 Size: 1142 757 BstXI CCAn_nnnn'nTGG Cuts at: 0 1581 1899 Size: 1581 318 BstYI r'GATC_y Cuts at: 0 533 1266 1899 Size: 533 733 633 Fragments arranged by size: 733 633 533 BtsI GCAGTG_nn' Cuts at: 0 973 1202 1534 1574 1899 Size: 973 229 332 40 325 Fragments arranged by size: 973 332 325 229 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1899 Size: 1001 898 EcoNI CCTnn'n_nnAGG Cuts at: 0 27 489 1899 Size: 27 462 1410 Fragments arranged by size: 1410 462 27 EcoO109I rG'GnC_Cy Cuts at: 0 112 1899 Size: 112 1787 EcoRI G'AATT_C Cuts at: 0 628 1899 Size: 628 1271 EcoRV GAT'ATC Cuts at: 0 435 1899 Size: 435 1464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 475 1899 Size: 475 1424 HaeII r_GCGC'y Cuts at: 0 79 1899 Size: 79 1820 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 424 456 1899 Size: 424 32 1443 Fragments arranged by size: 1443 424 32 HincII GTy'rAC Cuts at: 0 1063 1899 Size: 1063 836 HindIII A'AGCT_T Cuts at: 0 262 472 1899 Size: 262 210 1427 Fragments arranged by size: 1427 262 210 MfeI C'AATT_G Cuts at: 0 722 1743 1894 1899 Size: 722 1021 151 5 Fragments arranged by size: 1021 722 151 5 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1008 1899 Size: 1008 891 MslI CAynn'nnrTG Cuts at: 0 568 1579 1809 1899 Size: 568 1011 230 90 Fragments arranged by size: 1011 568 230 90 MspA1I CmG'CkG Cuts at: 0 59 1899 Size: 59 1840 NsiI A_TGCA'T Cuts at: 0 529 757 1597 1899 Size: 529 228 840 302 Fragments arranged by size: 840 529 302 228 NspI r_CATG'y Cuts at: 0 388 1899 Size: 388 1511 PciI A'CATG_T Cuts at: 0 384 1899 Size: 384 1515 PsiI TTA'TAA Cuts at: 0 740 1460 1899 Size: 740 720 439 Fragments arranged by size: 740 720 439 PspOMI G'GGCC_C Cuts at: 0 111 1899 Size: 111 1788 PstI C_TGCA'G Cuts at: 0 660 1899 Size: 660 1239 PvuII CAG'CTG Cuts at: 0 59 1899 Size: 59 1840 ScaI AGT'ACT Cuts at: 0 1863 1899 Size: 1863 36 SfcI C'TryA_G Cuts at: 0 656 1899 Size: 656 1243 SmlI C'TyrA_G Cuts at: 0 42 1899 Size: 42 1857 SpeI A'CTAG_T Cuts at: 0 648 1615 1899 Size: 648 967 284 Fragments arranged by size: 967 648 284 SspI AAT'ATT Cuts at: 0 1563 1899 Size: 1563 336 StuI AGG'CCT Cuts at: 0 22 1899 Size: 22 1877 StyI C'CwwG_G Cuts at: 0 268 687 1899 Size: 268 419 1212 Fragments arranged by size: 1212 419 268 TatI w'GTAC_w Cuts at: 0 553 1084 1861 1899 Size: 553 531 777 38 Fragments arranged by size: 777 553 531 38 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 965 997 1899 Size: 965 32 902 Fragments arranged by size: 965 902 32 XmnI GAAnn'nnTTC Cuts at: 0 256 1889 1899 Size: 256 1633 10 Fragments arranged by size: 1633 256 10 Enzymes that do cut: AccI AflIII AlwNI ApaI ApoI BanI BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtsI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I NsiI NspI PciI PsiI PspOMI PstI PvuII ScaI SfcI SmlI SpeI SspI StuI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BclI BmgBI BmtI BplI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI FseI FspI FspAI HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI