################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccIII AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BcnI BglI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI Hin1I KasI KspI MfeI MluI MluNI MroI MscI MunI NarI NciI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 265 GGTACC G^GTACC AflII 811 CTTAAG C^TTAAG AlwNI 884 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 144 CCTAAGG CC^T[ACGT]AGG Asp718I 265 GGTACC G^GTACC AspI 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1118 CTCGAG C^[CT]CG[GA]G AvaII 544 GGTCC G^G[AT]CC BanI 265 GGTACC G^G[CT][GA]CC BciVI 139 GGATAC GTATCC(6/5) BfrI 811 CTTAAG C^TTAAG BglII 1821 AGATCT A^GATCT BpmI 1664 CTCCAG CTGGAG(16/14) BsaAI 1378 TACGTG [CT]AC^GT[GA] BsiEI 1280 CGACCG CG[GA][CT]^CG BsmBI 2470 CGTCTC CGTCTC(1/5) BsoBI 1118 CTCGAG C^[CT]CG[GA]G Bsp1407I 2010 TGTACA T^GTACA BspHI 836 TCATGA T^CATGA BspMI 2112 GCAGGT ACCTGC(4/8) BsrFI 2033 GCCGGC [GA]^CCGG[CT] BsrGI 2010 TGTACA T^GTACA BstAPI 637 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2189 CCACCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 144 CCTAAGG CC^T[ACGT]AGG CfoI 2215 GCGC GCG^C Cfr10I 2033 GCCGGC [GA]^CCGG[CT] DraIII 1193 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2343 GACTTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2507 CCATGG C^C[GA][CT]GG EaeI 2466 TGGCCG [CT]^GGCC[GA] Eco81I 144 CCTAAGG CC^T[ACGT]AGG EcoRV 1793 GATATC GAT^ATC HapII 2034 CCGG C^CGG HhaI 2215 GCGC GCG^C HinP1I 2215 GCGC G^CGC HpaI 1183 GTTAAC GTT^AAC HpaII 2034 CCGG C^CGG KpnI 265 GGTACC GGTAC^C MspA1I 2392 CAGCTG C[AC]G^C[GT]G MspI 2034 CCGG C^CGG NaeI 2033 GCCGGC GCC^GGC NcoI 2507 CCATGG C^CATGG NdeI 741 CATATG CA^TATG NgoMIV 2033 GCCGGC G^CCGGC PaeR7I 1118 CTCGAG C^TCGAG PflFI 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2505 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 2392 CAGCTG CAG^CTG RcaI 836 TCATGA T^CATGA SacI 185 GAGCTC GAGCT^C ScaI 231 AGTACT AGT^ACT SpeI 696 ACTAGT A^CTAGT SspBI 2010 TGTACA T^GTACA StuI 2626 AGGCCT AGG^CCT Tth111I 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 462 CCAGTCTATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1118 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 1559 CGCG CG^CG 2214 CGCG AclI 1108 AACGTT AA^CGTT 1234 AACGTT AspHI 185 GAGCTC G[AT]GC[AT]^C 1754 GTGCTC BanII 185 GAGCTC G[GA]GC[CT]^C 855 GAGCCC BsaBI 1537 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1711 GATGATGATC BsiHKAI 185 GAGCTC G[AT]GC[AT]^C 1754 GTGCTC BsmFI 1078 GGGAC GGGAC(10/14) 1309 GTCCC BsmI 1553 GCATTC GAATGC(1/-1) 1799 GCATTC BstUI 1559 CGCG CG^CG 2214 CGCG DraI 10 TTTAAA TTT^AAA 1020 TTTAAA HincII 88 GTTGAC GT[CT]^[GA]AC 1183 GTTAAC HindII 88 GTTGAC GT[CT]^[GA]AC 1183 GTTAAC MamI 1537 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1711 GATGATGATC MslI 956 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2508 CATGGTCATG MvnI 1559 CGCG CG^CG 2214 CGCG NspI 929 ACATGC [GA]CATG^[CT] 1897 GCATGT PflMI 462 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 597 CCAACAAGTGG Psp1406I 1108 AACGTT AA^CGTT 1234 AACGTT PstI 1336 CTGCAG CTGCA^G 2612 CTGCAG Van91I 462 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 597 CCAACAAGTGG XbaI 207 TCTAGA T^CTAGA 298 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI BbsI BbvI BclI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII