Restriction map for
pFN21AE3353
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# NO CUT ENZYMES ( 68 enzymes) #
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AccIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AvrII BamHI
BcgI BciVI BclI BlnI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 2392 GACGTC GACGT^C
Acc65I 541 GGTACC G^GTACC
AccI 88 GTCTAC GT^[AC][GT]AC
AclI 577 AACGTT AA^CGTT
AflII 1294 CTTAAG C^TTAAG
Alw44I 2244 GTGCAC G^TGCAC
AocI 2421 CCTGAGG CC^T[ACGT]AGG
ApaLI 2244 GTGCAC G^TGCAC
Asp718I 541 GGTACC G^GTACC
AviII 1376 TGCGCA TGC^GCA
BbeI 2903 GGCGCC GGCGC^C
BbrPI 1966 CACGTG CAC^GTG
BbvCI 123 CCTCAGC CCTCAGC(-5/-2)
BfrI 1294 CTTAAG C^TTAAG
BglII 1469 AGATCT A^GATCT
BsmAI 1166 GTCTC GTCTC(1/5)
BsmBI 1165 CGTCTC CGTCTC(1/5)
Bsp1407I 2285 TGTACA T^GTACA
BspHI 2696 TCATGA T^CATGA
BspLU11I 1319 ACATGT A^CATGT
BsrFI 458 GCCGGC [GA]^CCGG[CT]
BsrGI 2285 TGTACA T^GTACA
BstEII 1653 GGTCACC G^GT[ACGT]ACC
BstPI 1653 GGTCACC G^GT[ACGT]ACC
BstYI 1469 AGATCT [GA]^GATC[CT]
Bsu36I 2421 CCTGAGG CC^T[ACGT]AGG
Cfr10I 458 GCCGGC [GA]^CCGG[CT]
EagI 449 CGGCCG C^GGCCG
EclXI 449 CGGCCG C^GGCCG
Eco52I 449 CGGCCG C^GGCCG
Eco81I 2421 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1653 GGTCACC G^GT[ACGT]ACC
EcoRI 28 GAATTC G^AATTC
FspI 1376 TGCGCA TGC^GCA
HgaI 261 GCGTC GACGC(5/10)
KasI 2903 GGCGCC G^GCGCC
KpnI 541 GGTACC GGTAC^C
MflI 1469 AGATCT [GA]^GATC[CT]
NaeI 458 GCCGGC GCC^GGC
NarI 2903 GGCGCC GG^CGCC
NdeI 2657 CATATG CA^TATG
NgoMIV 458 GCCGGC G^CCGGC
NheI 1635 GCTAGC G^CTAGC
NotI 448 GCGGCCGC GC^GGCCGC
PmaCI 1966 CACGTG CAC^GTG
PmlI 1966 CACGTG CAC^GTG
PpuMI 1583 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 822 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 577 AACGTT AA^CGTT
PvuI 2676 CGATCG CGAT^CG
RcaI 2696 TCATGA T^CATGA
SapI 171 GCTCTTC GCTCTTC(1/4)
SexAI 1586 ACCTGGT A^CC[AT]GGT
SfoI 2903 GGCGCC GGC^GCC
SmaI 2589 CCCGGG CCC^GGG
SphI 2153 GCATGC GCATG^C
SspBI 2285 TGTACA T^GTACA
XhoII 1469 AGATCT [GA]^GATC[CT]
XmaCI 2589 CCCGGG C^CCGGG
XmaI 2589 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 1319 ACATGT A^C[GA][CT]GT
3110 ACGTGT
ApaI 563 GGGCCC GGGCC^C
1907 GGGCCC
Asp700I 2085 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3036 GAAGCACTTC
BglI 1201 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1769 GCCACGTAGGC
BlpI 2100 GCTGAGC GC^T[ACGT]AGC
2964 GCTCAGC
Bpu1102I 2100 GCTGAGC GC^T[ACGT]AGC
2964 GCTCAGC
BsaAI 1771 CACGTA [CT]AC^GT[GA]
1966 CACGTG
BsrDI 1251 CATTGC GCAATG(2/0)
113 GCAATG
BssSI 376 CACGAG CACGAG(-5/-1)
1576 CACGAG
BstAPI 1839 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2355 GCAGGACATGC
CelII 2100 GCTGAGC GC^T[ACGT]AGC
2964 GCTCAGC
EarI 172 CTCTTC CTCTTC(1/4)
2917 CTCTTC
Ksp632I 172 CTCTTC CTCTTC(1/4)
2917 CTCTTC
NcoI 23 CCATGG C^CATGG
824 CCATGG
XmnI 2085 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3036 GAAGCACTTC
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI