################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI HapII Hin1I HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 638 GTAGAC GT^[AC][GT]AC Alw44I 299 GTGCAC G^TGCAC AocI 1003 CCTGAGG CC^T[ACGT]AGG Aor51HI 1187 AGCGCT AGC^GCT ApaLI 299 GTGCAC G^TGCAC BciVI 2290 GTATCC GTATCC(6/5) BclI 1845 TGATCA T^GATCA BsaAI 388 CACGTA [CT]AC^GT[GA] BsiYI 1003 CCTGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1003 CCTGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 693 GTCCC GGGAC(10/14) BsmI 2067 GCATTC GAATGC(1/-1) Bsp1407I 449 TGTACA T^GTACA BspMI 2595 ACCTGC ACCTGC(4/8) BsrGI 449 TGTACA T^GTACA BssSI 2546 CACGAG CACGAG(-5/-1) BstBI 2060 TTCGAA TT^CGAA BstF5I 1333 CATCC GGATG(2/0) Bsu36I 1003 CCTGAGG CC^T[ACGT]AGG Cac8I 1294 GCAAGC GC[ACGT]^[ACGT]GC CfoI 1188 GCGC GCG^C Eco47III 1187 AGCGCT AGC^GCT Eco81I 1003 CCTGAGG CC^T[ACGT]AGG EcoT14I 1962 CCTTGG C^C[AT][AT]GG EcoT22I 2053 ATGCAT ATGCA^T FbaI 1845 TGATCA T^GATCA FokI 1333 CATCC GGATG(9/13) HaeII 1187 AGCGCT [GA]GCGC^[CT] HaeIII 2674 GGCC GG^CC HgaI 2475 GCGTC GACGC(5/10) HhaI 1188 GCGC GCG^C HinP1I 1188 GCGC G^CGC HindIII 184 AAGCTT A^AGCTT HpaI 1041 GTTAAC GTT^AAC MspA1I 2552 CAGCGG C[AC]G^C[GT]G NdeI 1441 CATATG CA^TATG NsiI 2053 ATGCAT ATGCA^T NspI 1317 GCATGT [GA]CATG^[CT] NspV 2060 TTCGAA TT^CGAA PstI 116 CTGCAG CTGCA^G SapI 139 GCTCTTC GCTCTTC(1/4) SexAI 2141 ACCTGGT A^CC[AT]GGT SfuI 2060 TTCGAA TT^CGAA SspBI 449 TGTACA T^GTACA StyI 1962 CCTTGG C^C[AT][AT]GG SwaI 227 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 299 GTGCAC G[AT]GC[AT]^C 1261 GAGCAC BbvI 2551 GCAGC GCAGC(8/12) 481 GCTGC BcgI 528 CGAAAATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1743 GCAAAGTTTTCG BsiHKAI 299 GTGCAC G[AT]GC[AT]^C 1261 GAGCAC BstYI 38 AGATCC [GA]^GATC[CT] 2637 AGATCC EarI 140 CTCTTC CTCTTC(1/4) 6 GAAGAG Fnu4HI 481 GCTGC GC^[ACGT]GC 2551 GCAGC HincII 1041 GTTAAC GT[CT]^[GA]AC 2532 GTCAAC HindII 1041 GTTAAC GT[CT]^[GA]AC 2532 GTCAAC ItaI 481 GCTGC GC^[ACGT]GC 2551 GCAGC Ksp632I 140 CTCTTC CTCTTC(1/4) 6 GAAGAG MaeII 389 ACGT A^CGT 1602 ACGT MflI 38 AGATCC [GA]^GATC[CT] 2637 AGATCC MwoI 2554 GCGGATTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 2675 GCCTTGATAGC SmlI 31 CTTGAG C^T[CT][GA]AG 1457 CTCAAG TseI 481 GCTGC G^C[AT]GC 2551 GCAGC XhoII 38 AGATCC [GA]^GATC[CT] 2637 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbsI BfaI BmyI BpmI BpuAI BsaJI BseRI Bsp1286I BspHI BsrI BssKI BstNI Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV PpuMI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XmnI