(Circular) (Six-base) MAPSORT of: pFN21AE4244.seq Check: 7103 from: 1 to: 7432 pFN21AE4244 7432 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5242 278 Size: 53 83 186 936 3706 2468 Fragments arranged by size: 3706 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2644 4775 1937 Size: 707 2131 4594 Fragments arranged by size: 4594 2131 707 Acc65I G'GTAC_C Cuts at: 4753 4753 Size: 7432 AclI AA'CG_TT Cuts at: 1563 5559 5932 1563 Size: 3996 373 3063 Fragments arranged by size: 3996 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2160 2937 4437 5495 6879 7147 1632 Size: 423 105 777 1500 1058 1384 268 1917 Fragments arranged by size: 1917 1500 1384 1058 777 423 268 105 AfeI AGC'GCT Cuts at: 3194 3194 Size: 7432 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7235 AflIII A'CryG_T Cuts at: 1203 6331 7308 1203 Size: 5128 977 1327 Fragments arranged by size: 5128 1327 977 AgeI A'CCGG_T Cuts at: 6263 6263 Size: 7432 AhdI GACnn_n'nnGTC Cuts at: 6161 6161 Size: 7432 AleI CACnn'nnGTG Cuts at: 1166 3144 3156 1166 Size: 1978 12 5442 Fragments arranged by size: 5442 1978 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4209 1184 Size: 3025 4407 AlwNI CAG_nnn'CTG Cuts at: 5175 6747 5175 Size: 1572 5860 ApaLI G'TGCA_C Cuts at: 2304 5489 6645 2304 Size: 3185 1156 3091 Fragments arranged by size: 3185 3091 1156 ApoI r'AATT_y Cuts at: 1493 2218 2297 2426 2444 2492 2633 2854 3251 Size: 725 79 129 18 48 141 221 397 Cuts at: 3251 3356 3598 4029 4101 4741 4967 5119 7228 Size: 105 242 431 72 640 226 152 2109 Cuts at: 7228 1493 Size: 1697 Fragments arranged by size: 2109 1697 725 640 431 397 242 226 221 152 141 129 105 79 72 48 18 AseI AT'TA_AT Cuts at: 160 1032 5986 160 Size: 872 4954 1606 Fragments arranged by size: 4954 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7432 AvaI C'yCGr_G Cuts at: 1870 1943 4757 1870 Size: 73 2814 4545 Fragments arranged by size: 4545 2814 73 BamHI G'GATC_C Cuts at: 4762 5129 4762 Size: 367 7065 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4753 6208 618 Size: 325 217 627 2966 1455 1842 Fragments arranged by size: 2966 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4751 1391 Size: 3360 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2614 3314 3380 3433 928 Size: 971 235 480 700 66 53 4927 Fragments arranged by size: 4927 971 700 480 235 66 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2522 2556 3737 3771 5623 5657 1903 Size: 34 585 34 1181 34 1852 34 3678 Fragments arranged by size: 3678 1852 1181 585 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4306 5324 6534 4306 Size: 1018 1210 5204 Fragments arranged by size: 5204 1210 1018 BclI T'GATC_A Cuts at: 1548 3850 1548 Size: 2302 5130 BfrBI ATG'CAT Cuts at: 4060 4060 Size: 7432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6043 136 Size: 107 122 71 631 605 4371 1525 Fragments arranged by size: 4371 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7427 7427 Size: 7432 BlpI GC'TnA_GC Cuts at: 4849 4849 Size: 7432 Bme1580I G_kGCm'C Cuts at: 1790 2308 2783 3278 5493 6649 1790 Size: 518 475 495 2215 1156 2573 Fragments arranged by size: 2573 2215 1156 518 495 475 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4613 4685 5163 Size: 416 53 83 399 24 3196 72 478 Cuts at: 5163 6121 442 Size: 958 1753 Fragments arranged by size: 3196 1753 958 478 416 399 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7432 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 2756 3036 6092 1239 Size: 921 596 280 3056 2579 Fragments arranged by size: 3056 2579 921 596 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 3447 5557 6422 6720 2057 Size: 1390 2110 865 298 2769 Fragments arranged by size: 2769 2110 1390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6095 882 Size: 617 4596 2219 Fragments arranged by size: 4596 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 5223 493 Size: 711 1191 2828 2702 Fragments arranged by size: 2828 2702 1191 711 BsaBI GATnn'nnATC Cuts at: 7426 7426 Size: 7432 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5239 5621 6256 275 Size: 53 83 186 936 3706 382 635 1451 Fragments arranged by size: 3706 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5865 6263 6537 6684 7095 5865 Size: 398 274 147 411 6202 Fragments arranged by size: 6202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2927 2957 3257 3287 3963 Size: 30 165 30 1524 30 300 30 676 Cuts at: 3963 3993 4576 4606 1178 Size: 30 583 30 4004 Fragments arranged by size: 4004 1524 676 583 300 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2948 2951 4584 1186 Size: 843 919 3 1633 4034 Fragments arranged by size: 4034 1633 919 843 3 BseYI C'CCAG_C Cuts at: 6635 7131 6635 Size: 496 6936 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6859 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4882 5643 6671 1540 Size: 275 186 2881 761 1028 2301 Fragments arranged by size: 2881 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2308 3270 5493 5578 6649 2308 Size: 962 2223 85 1071 3091 Fragments arranged by size: 3091 2223 1071 962 85 BsmI GAATG_Cn' Cuts at: 4072 4943 5036 4072 Size: 871 93 6468 Fragments arranged by size: 6468 871 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2308 2783 3270 3278 4751 5493 5578 Size: 399 518 475 487 8 1473 742 85 Cuts at: 5578 6649 1391 Size: 1071 2174 Fragments arranged by size: 2174 1473 1071 742 518 487 475 399 85 8 BspEI T'CCGG_A Cuts at: 7095 7095 Size: 7432 BspHI T'CATG_A Cuts at: 3037 3796 3847 4243 4510 5321 7051 3037 Size: 759 51 396 267 811 1730 3418 Fragments arranged by size: 3418 1730 811 759 396 267 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4609 4787 844 Size: 3765 178 3489 Fragments arranged by size: 3765 3489 178 BsrBI CCG'CTC Cuts at: 1394 1823 5319 1394 Size: 429 3496 3507 Fragments arranged by size: 3507 3496 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5927 6101 65 Size: 1183 4679 174 1396 Fragments arranged by size: 4679 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6076 6263 1700 Size: 110 4266 187 2869 Fragments arranged by size: 4266 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2454 96 Size: 2358 5074 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7432 BssSI C'ACGA_G Cuts at: 4551 5492 6504 4551 Size: 941 1012 5479 Fragments arranged by size: 5479 1012 941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7432 BstBI TT'CG_AA Cuts at: 4066 6236 4066 Size: 2170 5262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7432 BstYI r'GATC_y Cuts at: 1972 2043 4642 4762 5129 5514 5531 6983 7069 Size: 71 2599 120 367 385 17 1452 86 Cuts at: 7069 7081 7427 1972 Size: 12 346 1977 Fragments arranged by size: 2599 1977 1452 385 367 346 120 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3009 3009 Size: 7432 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6880 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2820 3834 487 Size: 683 56 1594 1014 4085 Fragments arranged by size: 4085 1594 1014 683 56 BtsI GCAGTG_nn' Cuts at: 2094 2520 3095 4948 5742 5762 2094 Size: 426 575 1853 794 20 3764 Fragments arranged by size: 3764 1853 794 575 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7432 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7397 DraI TTT'AAA Cuts at: 2235 3505 4036 4736 2235 Size: 1270 531 700 4931 Fragments arranged by size: 4931 1270 700 531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6439 809 Size: 507 5123 1802 Fragments arranged by size: 5123 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4879 5768 7397 Size: 54 1425 50 275 18 3049 889 1629 Cuts at: 7397 8 Size: 43 Fragments arranged by size: 3049 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4879 1537 Size: 275 3067 4090 Fragments arranged by size: 4090 3067 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2151 5362 1353 Size: 653 145 3211 3423 Fragments arranged by size: 3423 3211 653 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6019 6393 6539 387 Size: 1064 138 4430 374 146 1280 Fragments arranged by size: 4430 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2160 2756 2937 3036 4437 5495 Size: 393 423 105 596 181 99 1401 1058 Cuts at: 5495 6092 6879 7147 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1401 1058 787 597 596 423 393 268 181 105 99 EcoO109I rG'GnC_Cy Cuts at: 2697 2893 3551 2697 Size: 196 658 6578 Fragments arranged by size: 6578 658 196 EcoRI G'AATT_C Cuts at: 4741 5119 4741 Size: 378 7054 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3702 3702 Size: 7432 FspI TGC'GCA Cuts at: 5938 5938 Size: 7432 HaeII r_GCGC'y Cuts at: 1678 3196 6579 1678 Size: 1518 3383 2531 Fragments arranged by size: 3383 2531 1518 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 2347 2379 2927 Size: 32 135 32 526 32 217 32 548 Cuts at: 2927 2939 2959 2971 3322 3354 4301 4333 4505 Size: 12 20 12 351 32 947 32 172 Cuts at: 4505 4537 6073 6105 6147 6179 1373 Size: 32 1536 32 42 32 2626 Fragments arranged by size: 2626 1536 947 548 526 351 217 172 135 42 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3048 4539 4776 5022 669 Size: 988 281 1110 1491 237 246 3079 Fragments arranged by size: 3079 1491 1110 988 281 246 237 HindIII A'AGCT_T Cuts at: 748 2189 748 Size: 1441 5991 HpaI GTT'AAC Cuts at: 3048 5022 3048 Size: 1974 5458 KpnI G_GTAC'C Cuts at: 4757 4757 Size: 7432 MfeI C'AATT_G Cuts at: 5031 5031 Size: 7432 MluI A'CGCG_T Cuts at: 7308 7308 Size: 7432 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3371 4362 6546 6730 1305 Size: 2066 991 2184 184 2007 Fragments arranged by size: 2184 2066 2007 991 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5610 Fragments arranged by size: 5610 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2991 3144 3156 3240 3798 3882 3900 Size: 648 1825 153 12 84 558 84 18 Cuts at: 3900 4011 4077 4093 4161 4191 4242 5390 5749 Size: 111 66 16 68 30 51 1148 359 Cuts at: 5749 5908 518 Size: 159 2042 Fragments arranged by size: 2042 1825 1148 648 558 359 159 153 111 84 84 68 66 51 30 18 16 12 MspA1I CmG'CkG Cuts at: 1606 4559 4848 5525 6673 6918 1606 Size: 2953 289 677 1148 245 2120 Fragments arranged by size: 2953 2120 1148 677 289 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6880 NdeI CA'TA_TG Cuts at: 387 3447 387 Size: 3060 4372 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7432 NotI GC'GGCC_GC Cuts at: 4879 4879 Size: 7432 NsiI A_TGCA'T Cuts at: 4062 4062 Size: 7432 NspI r_CATG'y Cuts at: 1132 1229 3326 4790 6335 1132 Size: 97 2097 1464 1545 2229 Fragments arranged by size: 2229 2097 1545 1464 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7432 PciI A'CATG_T Cuts at: 6331 6331 Size: 7432 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7432 PmeI GTTT'AAAC Cuts at: 4736 4736 Size: 7432 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5501 5533 7014 7046 5501 Size: 32 1481 32 5887 Fragments arranged by size: 5887 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2697 2893 3551 2697 Size: 196 658 6578 Fragments arranged by size: 6578 658 196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7432 PsiI TTA'TAA Cuts at: 2658 5002 2658 Size: 2344 5088 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3018 3050 4425 4457 3018 Size: 32 1375 32 5993 Fragments arranged by size: 5993 1375 32 32 PstI C_TGCA'G Cuts at: 830 2125 4784 830 Size: 1295 2659 3478 Fragments arranged by size: 3478 2659 1295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7432 SalI G'TCGA_C Cuts at: 1936 4774 1936 Size: 2838 4594 SapI GCTCTTCn'nnn_ Cuts at: 2151 2151 Size: 7432 SbfI CC_TGCA'GG Cuts at: 4784 4784 Size: 7432 ScaI AGT'ACT Cuts at: 2954 4276 4313 5680 7392 2954 Size: 1322 37 1367 1712 2994 Fragments arranged by size: 2994 1712 1367 1322 37 SexAI A'CCwGG_T Cuts at: 4146 4146 Size: 7432 SfcI C'TryA_G Cuts at: 826 1045 2121 2638 3024 4780 5915 6596 6787 Size: 219 1076 517 386 1756 1135 681 191 Cuts at: 6787 826 Size: 1471 Fragments arranged by size: 1756 1471 1135 1076 681 517 386 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7432 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7432 SmaI CCC'GGG Cuts at: 1872 4759 1872 Size: 2887 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 3462 5536 6437 6699 820 Size: 197 926 93 1426 2074 901 262 1553 Fragments arranged by size: 2074 1553 1426 926 901 262 197 93 SnaBI TAC'GTA Cuts at: 493 5223 493 Size: 4730 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7432 SphI G_CATG'C Cuts at: 1132 4790 1132 Size: 3658 3774 SspI AAT'ATT Cuts at: 5 52 2231 3084 4708 5356 5 Size: 47 2179 853 1624 648 2081 Fragments arranged by size: 2179 2081 1624 853 648 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7432 StyI C'CwwG_G Cuts at: 513 1065 3967 4871 513 Size: 552 2902 904 3074 Fragments arranged by size: 3074 2902 904 552 SwaI ATTT'AAAT Cuts at: 2235 2235 Size: 7432 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3132 4632 5459 5476 5629 5814 6259 Size: 1255 1552 1500 827 17 153 185 445 Cuts at: 6259 325 Size: 1498 Fragments arranged by size: 1552 1500 1498 1255 827 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2454 2952 3620 Size: 274 80 33 51 1443 477 498 668 Cuts at: 3620 3790 4274 4311 5678 7390 96 Size: 170 484 37 1367 1712 138 Fragments arranged by size: 1712 1443 1367 668 498 484 477 274 170 138 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3257 3289 3963 3995 7392 7424 3257 Size: 32 674 32 3397 32 3265 Fragments arranged by size: 3397 3265 674 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7432 XbaI T'CTAG_A Cuts at: 4768 4768 Size: 7432 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7432 XmaI C'CCGG_G Cuts at: 1870 4757 1870 Size: 2887 4545 XmnI GAAnn'nnTTC Cuts at: 2145 2884 4072 5561 2145 Size: 739 1188 1489 4016 Fragments arranged by size: 4016 1489 1188 739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5240 276 Size: 53 83 186 936 3706 2468 Fragments arranged by size: 3706 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI XcmI