(Linear) (Six-base) MAPSORT of: KIEE4244.seq Check: 1757 from: 1 to: 2727 KIEE4244.orf With 182 enzymes: SgfI * July 3, 2012 18:51 .. AccI GT'mk_AC Cuts at: 0 639 2727 Size: 639 2088 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 155 932 2432 2727 Size: 50 105 777 1500 295 Fragments arranged by size: 1500 777 295 105 50 AfeI AGC'GCT Cuts at: 0 1189 2727 Size: 1189 1538 AleI CACnn'nnGTG Cuts at: 0 1139 1151 2727 Size: 1139 12 1576 Fragments arranged by size: 1576 1139 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2204 2727 Size: 2204 523 ApaLI G'TGCA_C Cuts at: 0 299 2727 Size: 299 2428 ApoI r'AATT_y Cuts at: 0 213 292 421 439 487 628 849 1246 Size: 213 79 129 18 48 141 221 397 Cuts at: 1246 1351 1593 2024 2096 2727 Size: 105 242 431 72 631 Fragments arranged by size: 631 431 397 242 221 213 141 129 105 79 72 48 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 129 609 1309 1375 1428 2727 Size: 129 480 700 66 53 1299 Fragments arranged by size: 1299 700 480 129 66 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 517 551 1732 1766 2727 Size: 517 34 1181 34 961 Fragments arranged by size: 1181 961 517 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2301 2727 Size: 2301 426 BclI T'GATC_A Cuts at: 0 1845 2727 Size: 1845 882 BfrBI ATG'CAT Cuts at: 0 2055 2727 Size: 2055 672 Bme1580I G_kGCm'C Cuts at: 0 303 778 1273 2727 Size: 303 475 495 1454 Fragments arranged by size: 1454 495 475 303 BmrI ACTGGGnnnn_n' Cuts at: 0 2608 2680 2727 Size: 2608 72 47 Fragments arranged by size: 2608 72 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 751 1031 2727 Size: 155 596 280 1696 Fragments arranged by size: 1696 596 280 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 1442 2727 Size: 52 1390 1285 Fragments arranged by size: 1390 1285 52 BsaAI yAC'GTr Cuts at: 0 390 2727 Size: 390 2337 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 922 952 1252 1282 1958 1988 2571 2601 Size: 922 30 300 30 676 30 583 30 Cuts at: 2601 2727 Size: 126 Fragments arranged by size: 922 676 583 300 126 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 24 943 946 2579 2727 Size: 24 919 3 1633 148 Fragments arranged by size: 1633 919 148 24 3 BsiHKAI G_wGCw'C Cuts at: 0 303 1265 2727 Size: 303 962 1462 Fragments arranged by size: 1462 962 303 BsmI GAATG_Cn' Cuts at: 0 2067 2727 Size: 2067 660 Bsp1286I G_dGCh'C Cuts at: 0 303 778 1265 1273 2727 Size: 303 475 487 8 1454 Fragments arranged by size: 1454 487 475 303 8 BspHI T'CATG_A Cuts at: 0 1032 1791 1842 2238 2505 2727 Size: 1032 759 51 396 267 222 Fragments arranged by size: 1032 759 396 267 222 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2604 2727 Size: 2604 123 BsrGI T'GTAC_A Cuts at: 0 449 2727 Size: 449 2278 BssSI C'ACGA_G Cuts at: 0 2546 2727 Size: 2546 181 BstBI TT'CG_AA Cuts at: 0 2061 2727 Size: 2061 666 BstYI r'GATC_y Cuts at: 0 38 2637 2727 Size: 38 2599 90 Fragments arranged by size: 2599 90 38 Bsu36I CC'TnA_GG Cuts at: 0 1004 2727 Size: 1004 1723 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 815 1829 2727 Size: 815 1014 898 Fragments arranged by size: 1014 898 815 BtsI GCAGTG_nn' Cuts at: 0 89 515 1090 2727 Size: 89 426 575 1637 Fragments arranged by size: 1637 575 426 89 DraI TTT'AAA Cuts at: 0 230 1500 2031 2727 Size: 230 1270 531 696 Fragments arranged by size: 1270 696 531 230 EarI CTCTTCn'nnn_ Cuts at: 0 1 146 2727 Size: 1 145 2581 Fragments arranged by size: 2581 145 1 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 155 751 932 1031 2432 2727 Size: 50 105 596 181 99 1401 295 Fragments arranged by size: 1401 596 295 181 105 99 50 EcoO109I rG'GnC_Cy Cuts at: 0 692 888 1546 2727 Size: 692 196 658 1181 Fragments arranged by size: 1181 692 658 196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1697 2727 Size: 1697 1030 HaeII r_GCGC'y Cuts at: 0 1191 2727 Size: 1191 1536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 93 125 342 374 922 934 954 966 Size: 93 32 217 32 548 12 20 12 Cuts at: 966 1317 1349 2296 2328 2500 2532 2727 Size: 351 32 947 32 172 32 195 Fragments arranged by size: 947 548 351 217 195 172 93 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 1043 2534 2727 Size: 1043 1491 193 Fragments arranged by size: 1491 1043 193 HindIII A'AGCT_T Cuts at: 0 184 2727 Size: 184 2543 HpaI GTT'AAC Cuts at: 0 1043 2727 Size: 1043 1684 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1366 2357 2727 Size: 1366 991 370 Fragments arranged by size: 1366 991 370 MslI CAynn'nnrTG Cuts at: 0 986 1139 1151 1235 1793 1877 1895 2006 Size: 986 153 12 84 558 84 18 111 Cuts at: 2006 2072 2088 2156 2186 2237 2727 Size: 66 16 68 30 51 490 Fragments arranged by size: 986 558 490 153 111 84 84 68 66 51 30 18 16 12 MspA1I CmG'CkG Cuts at: 0 2554 2727 Size: 2554 173 NdeI CA'TA_TG Cuts at: 0 1442 2727 Size: 1442 1285 NsiI A_TGCA'T Cuts at: 0 2057 2727 Size: 2057 670 NspI r_CATG'y Cuts at: 0 1321 2727 Size: 1321 1406 PpuMI rG'GwC_Cy Cuts at: 0 692 888 1546 2727 Size: 692 196 658 1181 Fragments arranged by size: 1181 692 658 196 PsiI TTA'TAA Cuts at: 0 653 2727 Size: 653 2074 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1013 1045 2420 2452 2727 Size: 1013 32 1375 32 275 Fragments arranged by size: 1375 1013 275 32 32 PstI C_TGCA'G Cuts at: 0 120 2727 Size: 120 2607 SapI GCTCTTCn'nnn_ Cuts at: 0 146 2727 Size: 146 2581 ScaI AGT'ACT Cuts at: 0 949 2271 2308 2727 Size: 949 1322 37 419 Fragments arranged by size: 1322 949 419 37 SexAI A'CCwGG_T Cuts at: 0 2141 2727 Size: 2141 586 SfcI C'TryA_G Cuts at: 0 116 633 1019 2727 Size: 116 517 386 1708 Fragments arranged by size: 1708 517 386 116 SmlI C'TyrA_G Cuts at: 0 31 1457 2727 Size: 31 1426 1270 Fragments arranged by size: 1426 1270 31 SspI AAT'ATT Cuts at: 0 226 1079 2703 2727 Size: 226 853 1624 24 Fragments arranged by size: 1624 853 226 24 StyI C'CwwG_G Cuts at: 0 1962 2727 Size: 1962 765 SwaI ATTT'AAAT Cuts at: 0 230 2727 Size: 230 2497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1127 2627 2727 Size: 1127 1500 100 Fragments arranged by size: 1500 1127 100 TatI w'GTAC_w Cuts at: 0 449 947 1615 1785 2269 2306 2727 Size: 449 498 668 170 484 37 421 Fragments arranged by size: 668 498 484 449 421 170 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1252 1284 1958 1990 2727 Size: 1252 32 674 32 737 Fragments arranged by size: 1252 737 674 32 32 XmnI GAAnn'nnTTC Cuts at: 0 140 879 2067 2727 Size: 140 739 1188 660 Fragments arranged by size: 1188 739 660 140 Enzymes that do cut: AccI AcuI AfeI AleI AlfI ApaLI ApoI BbsI BcgI BciVI BclI BfrBI Bme1580I BmrI BpmI BpuEI BsaAI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BssSI BstBI BstYI Bsu36I BtgZI BtsI DraI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI MmeI MslI MspA1I NdeI NsiI NspI PpuMI PsiI PsrI PstI SapI ScaI SexAI SfcI SmlI SspI StyI SwaI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI Tth111I XbaI XcmI XhoI XmaI ZraI