(Circular) (Six-base) MAPSORT of: pFN21AE3306.seq Check: 2117 from: 1 to: 7015 pFN21AE3306 7015 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3594 3594 Size: 7015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3626 4825 278 Size: 53 83 186 936 2090 1199 2468 Fragments arranged by size: 2468 2090 1199 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3285 4358 1937 Size: 1348 1073 4594 Fragments arranged by size: 4594 1348 1073 Acc65I G'GTAC_C Cuts at: 3735 4336 3735 Size: 601 6414 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3356 3722 3940 4259 4285 5078 6462 Size: 407 1317 366 218 319 26 793 1384 Cuts at: 6462 6730 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1317 793 407 366 319 268 218 26 AfeI AGC'GCT Cuts at: 3989 4069 3989 Size: 80 6935 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 3789 5914 6891 1203 Size: 2586 2125 977 1327 Fragments arranged by size: 2586 2125 1327 977 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 2755 3793 5744 2755 Size: 1038 1951 4026 Fragments arranged by size: 4026 1951 1038 AleI CACnn'nnGTG Cuts at: 1166 3782 1166 Size: 2616 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7015 AlwNI CAG_nnn'CTG Cuts at: 2658 2837 3193 3250 3568 4758 6330 2658 Size: 179 356 57 318 1190 1572 3343 Fragments arranged by size: 3343 1572 1190 356 318 179 57 ApaI G_GGCC'C Cuts at: 4295 4295 Size: 7015 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 2049 2113 4324 4550 4702 6811 1493 Size: 556 64 2211 226 152 2109 1697 Fragments arranged by size: 2211 2109 1697 556 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 3845 4052 4186 4340 1870 Size: 73 1902 207 134 154 4545 Fragments arranged by size: 4545 1902 207 154 134 73 BamHI G'GATC_C Cuts at: 3600 3692 4345 4712 3600 Size: 92 653 367 5903 Fragments arranged by size: 5903 653 367 92 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3269 3735 3761 4178 4336 Size: 325 217 627 1482 466 26 417 158 Cuts at: 4336 5791 618 Size: 1455 1842 Fragments arranged by size: 1842 1482 1455 627 466 417 325 217 158 26 BanII G_rGCy'C Cuts at: 1391 3183 3933 4000 4295 4334 1391 Size: 1792 750 67 295 39 4072 Fragments arranged by size: 4072 1792 750 295 67 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2220 2232 2798 3554 928 Size: 971 321 12 566 756 4389 Fragments arranged by size: 4389 971 756 566 321 12 BbvCI CC'TCA_GC Cuts at: 3377 4071 3377 Size: 694 6321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2261 2295 2348 2382 3143 3177 3973 Size: 34 324 34 53 34 761 34 796 Cuts at: 3973 4007 5206 5240 1903 Size: 34 1199 34 3678 Fragments arranged by size: 3678 1199 796 761 324 53 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4907 6117 4907 Size: 1210 5805 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3648 5626 136 Size: 107 122 71 631 605 1976 1978 1525 Fragments arranged by size: 1978 1976 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3462 7010 3462 Size: 3548 3467 BlpI GC'TnA_GC Cuts at: 3638 4432 3638 Size: 794 6221 Bme1580I G_kGCm'C Cuts at: 1790 3153 3272 4181 4295 5076 6232 1790 Size: 1363 119 909 114 781 1156 2573 Fragments arranged by size: 2573 1363 1156 909 781 119 114 BmgBI CAC'GTC Cuts at: 1318 3372 1318 Size: 2054 4961 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4746 5704 442 Size: 416 53 83 399 24 3329 958 1753 Fragments arranged by size: 3329 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2680 5675 1239 Size: 1441 2995 2579 Fragments arranged by size: 2995 2579 1441 Bpu10I CC'TnA_GC Cuts at: 2830 3377 4071 2830 Size: 547 694 5774 Fragments arranged by size: 5774 694 547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3167 3383 3482 3602 3641 4067 4292 5140 Size: 999 216 99 120 39 426 225 848 Cuts at: 5140 6005 6303 2168 Size: 865 298 2880 Fragments arranged by size: 2880 999 865 848 426 298 225 216 120 99 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2930 3506 4097 5678 882 Size: 617 1431 576 591 1581 2219 Fragments arranged by size: 2219 1581 1431 617 591 576 BsaAI yAC'GTr Cuts at: 493 1204 4140 4806 493 Size: 711 2936 666 2702 Fragments arranged by size: 2936 2702 711 666 BsaBI GATnn'nnATC Cuts at: 3467 7009 3467 Size: 3542 3473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3623 4822 5204 5839 Size: 53 83 186 936 2090 1199 382 635 Cuts at: 5839 275 Size: 1451 Fragments arranged by size: 2090 1451 1199 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5448 5846 6120 6267 6678 5448 Size: 398 274 147 411 5785 Fragments arranged by size: 5785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2872 2902 3230 3260 1178 Size: 30 165 30 1469 30 328 30 4933 Fragments arranged by size: 4933 1469 328 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2165 2247 2579 2864 2867 2870 2879 Size: 913 66 82 332 285 3 3 9 Cuts at: 2879 2882 2885 2915 2918 2921 2924 2939 2942 Size: 3 3 30 3 3 3 15 3 Cuts at: 2942 2945 2948 2969 3251 3278 4072 1186 Size: 3 3 21 282 27 794 4129 Fragments arranged by size: 4129 913 794 332 285 282 82 66 30 27 21 15 9 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2268 3405 3934 4222 6218 6714 2268 Size: 1137 529 288 1996 496 2569 Fragments arranged by size: 2569 1996 1137 529 496 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2589 3572 3590 1163 Size: 573 853 983 18 4588 Fragments arranged by size: 4588 983 853 573 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2681 2713 2805 3064 3720 3783 Size: 275 186 680 32 92 259 656 63 Cuts at: 3783 4465 5226 6254 1540 Size: 682 761 1028 2301 Fragments arranged by size: 2301 1028 761 682 680 656 275 259 186 92 63 32 BsiHKAI G_wGCw'C Cuts at: 2361 2885 3183 3492 3933 3966 5076 5161 6232 Size: 524 298 309 441 33 1110 85 1071 Cuts at: 6232 2361 Size: 3144 Fragments arranged by size: 3144 1110 1071 524 441 309 298 85 33 BsmI GAATG_Cn' Cuts at: 2446 4526 4619 2446 Size: 2080 93 4842 Fragments arranged by size: 4842 2080 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2700 4238 2700 Size: 1538 5477 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2885 3153 3183 3272 3492 3933 Size: 399 571 524 268 30 89 220 441 Cuts at: 3933 3966 4000 4181 4295 4334 5076 5161 6232 Size: 33 34 181 114 39 742 85 1071 Cuts at: 6232 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 571 524 441 399 268 220 181 114 89 85 39 34 33 30 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 4026 4904 6634 4026 Size: 878 1730 4407 Fragments arranged by size: 4407 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3213 3594 3639 4370 844 Size: 1916 453 381 45 731 3489 Fragments arranged by size: 3489 1916 731 453 381 45 BsrBI CCG'CTC Cuts at: 1394 1823 2497 2554 2569 2581 2692 4902 1394 Size: 429 674 57 15 12 111 2210 3507 Fragments arranged by size: 3507 2210 674 429 111 57 15 12 BsrDI GCAATG_nn' Cuts at: 65 1248 5510 5684 65 Size: 1183 4262 174 1396 Fragments arranged by size: 4262 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2939 3208 3647 5659 5846 1700 Size: 110 1129 269 439 2012 187 2869 Fragments arranged by size: 2869 2012 1129 439 269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 5075 6087 5075 Size: 1012 6003 BstAPI GCAn_nnn'nTGC Cuts at: 794 3193 794 Size: 2399 4616 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 3724 1184 Size: 2540 4475 BstXI CCAn_nnnn'nTGG Cuts at: 3784 3784 Size: 7015 BstYI r'GATC_y Cuts at: 1972 3462 3600 3692 3886 4345 4712 5097 5114 Size: 1490 138 92 194 459 367 385 17 Cuts at: 5114 6566 6652 6664 7010 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1490 1452 459 385 367 346 194 138 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4197 4197 Size: 7015 BtgI C'CryG_G Cuts at: 513 1065 2004 2684 3777 3783 4287 513 Size: 552 939 680 1093 6 504 3241 Fragments arranged by size: 3241 1093 939 680 552 504 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6276 Fragments arranged by size: 6276 683 56 BtsI GCAGTG_nn' Cuts at: 4531 5325 5345 4531 Size: 794 20 6201 Fragments arranged by size: 6201 794 20 ClaI AT'CG_AT Cuts at: 1554 3861 1554 Size: 2307 4708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6022 809 Size: 507 4706 1802 Fragments arranged by size: 4706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2678 2710 Size: 54 1425 50 275 18 423 425 32 Cuts at: 2710 2802 3780 4462 5351 6980 8 Size: 92 978 682 889 1629 43 Fragments arranged by size: 1629 1425 978 889 682 425 423 275 92 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2678 2710 2802 3780 4462 1537 Size: 275 866 32 92 978 682 4090 Fragments arranged by size: 4090 978 866 682 275 92 32 EarI CTCTTCn'nnn_ Cuts at: 1353 2203 2417 2724 2841 2856 2910 2916 4945 Size: 850 214 307 117 15 54 6 2029 Cuts at: 4945 1353 Size: 3423 Fragments arranged by size: 3423 2029 850 307 214 117 54 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2576 5602 5976 6122 387 Size: 1064 138 987 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 987 374 146 138 EcoICRI GAG'CTC Cuts at: 3181 3931 3181 Size: 750 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2680 3356 3722 3940 4259 4285 Size: 393 407 641 676 366 218 319 26 Cuts at: 4285 5078 5675 6462 6730 1239 Size: 793 597 787 268 1524 Fragments arranged by size: 1524 793 787 676 641 597 407 393 366 319 268 218 26 EcoNI CCTnn'n_nnAGG Cuts at: 2150 3134 4200 2150 Size: 984 1066 4965 Fragments arranged by size: 4965 1066 984 EcoO109I rG'GnC_Cy Cuts at: 2749 3894 4191 4278 4292 2749 Size: 1145 297 87 14 5472 Fragments arranged by size: 5472 1145 297 87 14 EcoRI G'AATT_C Cuts at: 4324 4702 4324 Size: 378 6637 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3376 3970 3376 Size: 594 6421 FspI TGC'GCA Cuts at: 5521 5521 Size: 7015 HaeII r_GCGC'y Cuts at: 1678 3129 3991 4071 6162 1678 Size: 1451 862 80 2091 2531 Fragments arranged by size: 2531 2091 1451 862 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2741 2773 2798 2830 2945 Size: 32 135 32 1169 32 25 32 115 Cuts at: 2945 2977 3677 3709 3779 3811 5656 5688 5730 Size: 32 700 32 70 32 1845 32 42 Cuts at: 5730 5762 1373 Size: 32 2626 Fragments arranged by size: 2626 1845 1169 700 135 115 70 42 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 4359 4605 669 Size: 988 281 2421 246 3079 Fragments arranged by size: 3079 2421 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7015 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KpnI G_GTAC'C Cuts at: 3739 4340 3739 Size: 601 6414 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2348 2784 3651 6129 6313 1305 Size: 1043 436 867 2478 184 2007 Fragments arranged by size: 2478 2007 1043 867 436 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 4770 Fragments arranged by size: 4770 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3473 3782 4973 5332 5491 518 Size: 648 2307 309 1191 359 159 2042 Fragments arranged by size: 2307 2042 1191 648 359 309 159 MspA1I CmG'CkG Cuts at: 1606 2533 2686 2819 3565 4222 4289 4431 5108 Size: 927 153 133 746 657 67 142 677 Cuts at: 5108 6256 6501 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 927 746 677 657 245 153 142 133 67 NaeI GCC'GGC Cuts at: 3210 3649 3210 Size: 439 6576 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5524 Fragments arranged by size: 5524 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NgoMIV G'CCGG_C Cuts at: 3208 3647 3208 Size: 439 6576 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 2339 3793 4373 5918 1132 Size: 97 1110 1454 580 1545 2229 Fragments arranged by size: 2229 1545 1454 1110 580 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7015 PciI A'CATG_T Cuts at: 3789 5914 3789 Size: 2125 4890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2658 3037 1108 Size: 1550 379 5086 Fragments arranged by size: 5086 1550 379 PfoI T'CCnGG_A Cuts at: 2450 2450 Size: 7015 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PmlI CAC'GTG Cuts at: 4140 4140 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3041 3073 5084 5116 6597 6629 3041 Size: 32 2011 32 1481 32 3427 Fragments arranged by size: 3427 2011 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 3894 2749 Size: 1145 5870 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PspOMI G'GGCC_C Cuts at: 4291 4291 Size: 7015 PstI C_TGCA'G Cuts at: 830 3219 4367 830 Size: 2389 1148 3478 Fragments arranged by size: 3478 2389 1148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3565 4222 3565 Size: 657 6358 SacI G_AGCT'C Cuts at: 3183 3933 3183 Size: 750 6265 SacII CC_GC'GG Cuts at: 2687 4290 2687 Size: 1603 5412 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SanDI GG'GwC_CC Cuts at: 3894 3894 Size: 7015 SbfI CC_TGCA'GG Cuts at: 3219 4367 3219 Size: 1148 5867 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 2632 3215 3286 3922 4213 4363 5498 Size: 219 1587 583 71 636 291 150 1135 Cuts at: 5498 6179 6370 826 Size: 681 191 1471 Fragments arranged by size: 1587 1471 1135 681 636 583 291 219 191 150 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 3847 4054 4342 1872 Size: 1975 207 288 4545 Fragments arranged by size: 4545 1975 288 207 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 3182 3398 3497 3617 3656 Size: 197 926 204 1035 216 99 120 39 Cuts at: 3656 4082 4307 5119 6020 6282 820 Size: 426 225 812 901 262 1553 Fragments arranged by size: 1553 1035 926 901 812 426 262 225 216 204 197 120 99 39 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 2339 4373 1132 Size: 1207 2034 3774 Fragments arranged by size: 3774 2034 1207 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 3707 4206 1517 Size: 2190 499 4326 Fragments arranged by size: 4326 2190 499 StyI C'CwwG_G Cuts at: 513 1065 2004 3765 4454 513 Size: 552 939 1761 689 3074 Fragments arranged by size: 3074 1761 939 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3078 3092 3653 4214 5042 5059 Size: 1255 417 1081 14 561 561 828 17 Cuts at: 5059 5212 5397 5842 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1081 828 561 561 445 417 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5261 6973 96 Size: 274 80 33 51 1443 3284 1712 138 Fragments arranged by size: 3284 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 3230 3262 3886 3918 6975 7007 2872 Size: 32 326 32 624 32 3057 32 2880 Fragments arranged by size: 3057 2880 624 326 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7015 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3569 3569 Size: 7015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 3845 4052 4340 1870 Size: 1975 207 288 4545 Fragments arranged by size: 4545 1975 288 207 XmnI GAAnn'nnTTC Cuts at: 2052 2292 4034 5144 2052 Size: 240 1742 1110 3923 Fragments arranged by size: 3923 1742 1110 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3624 4823 276 Size: 53 83 186 936 2090 1199 2468 Fragments arranged by size: 2468 2090 1199 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI