(Linear) (Six-base) MAPSORT of: KIEE3306.seq Check: 5276 from: 1 to: 2310 KIEE3306.orf With 182 enzymes: SgfI * July 3, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1589 2310 Size: 1589 721 AatII G_ACGT'C Cuts at: 0 1621 2310 Size: 1621 689 AccI GT'mk_AC Cuts at: 0 1280 2310 Size: 1280 1030 Acc65I G'GTAC_C Cuts at: 0 1730 2310 Size: 1730 580 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1351 1717 1935 2254 2280 2310 Size: 34 1317 366 218 319 26 30 Fragments arranged by size: 1317 366 319 218 34 30 26 AfeI AGC'GCT Cuts at: 0 1984 2064 2310 Size: 1984 80 246 Fragments arranged by size: 1984 246 80 AflIII A'CryG_T Cuts at: 0 1784 2310 Size: 1784 526 AhdI GACnn_n'nnGTC Cuts at: 0 750 1788 2310 Size: 750 1038 522 Fragments arranged by size: 1038 750 522 AleI CACnn'nnGTG Cuts at: 0 1777 2310 Size: 1777 533 AlwNI CAG_nnn'CTG Cuts at: 0 653 832 1188 1245 1563 2310 Size: 653 179 356 57 318 747 Fragments arranged by size: 747 653 356 318 179 57 ApaI G_GGCC'C Cuts at: 0 2290 2310 Size: 2290 20 ApoI r'AATT_y Cuts at: 0 44 108 2310 Size: 44 64 2202 Fragments arranged by size: 2202 64 44 AvaI C'yCGr_G Cuts at: 0 1840 2047 2181 2310 Size: 1840 207 134 129 Fragments arranged by size: 1840 207 134 129 BamHI G'GATC_C Cuts at: 0 1595 1687 2310 Size: 1595 92 623 Fragments arranged by size: 1595 623 92 BanI G'GyrC_C Cuts at: 0 1264 1730 1756 2173 2310 Size: 1264 466 26 417 137 Fragments arranged by size: 1264 466 417 137 26 BanII G_rGCy'C Cuts at: 0 1178 1928 1995 2290 2310 Size: 1178 750 67 295 20 Fragments arranged by size: 1178 750 295 67 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 215 227 793 1549 2310 Size: 215 12 566 756 761 Fragments arranged by size: 761 756 566 215 12 BbvCI CC'TCA_GC Cuts at: 0 1372 2066 2310 Size: 1372 694 244 Fragments arranged by size: 1372 694 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 256 290 343 377 1138 1172 1968 2002 Size: 256 34 53 34 761 34 796 34 Cuts at: 2002 2310 Size: 308 Fragments arranged by size: 796 761 308 256 53 34 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1643 2310 Size: 1643 667 BglII A'GATC_T Cuts at: 0 1457 2310 Size: 1457 853 BlpI GC'TnA_GC Cuts at: 0 1633 2310 Size: 1633 677 Bme1580I G_kGCm'C Cuts at: 0 1148 1267 2176 2290 2310 Size: 1148 119 909 114 20 Fragments arranged by size: 1148 909 119 114 20 BmgBI CAC'GTC Cuts at: 0 1367 2310 Size: 1367 943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 2310 Size: 675 1635 Bpu10I CC'TnA_GC Cuts at: 0 825 1372 2066 2310 Size: 825 547 694 244 Fragments arranged by size: 825 694 547 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1162 1378 1477 1597 1636 2062 2287 Size: 163 999 216 99 120 39 426 225 Cuts at: 2287 2310 Size: 23 Fragments arranged by size: 999 426 225 216 163 120 99 39 23 BsaI GGTCTCn'nnnn_ Cuts at: 0 925 1501 2092 2310 Size: 925 576 591 218 Fragments arranged by size: 925 591 576 218 BsaAI yAC'GTr Cuts at: 0 2135 2310 Size: 2135 175 BsaBI GATnn'nnATC Cuts at: 0 1462 2310 Size: 1462 848 BsaHI Gr'CG_yC Cuts at: 0 1618 2310 Size: 1618 692 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 867 897 1225 1255 2310 Size: 867 30 328 30 1055 Fragments arranged by size: 1055 867 328 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 160 242 574 859 862 865 874 Size: 94 66 82 332 285 3 3 9 Cuts at: 874 877 880 910 913 916 919 934 937 Size: 3 3 30 3 3 3 15 3 Cuts at: 937 940 943 964 1246 1273 2067 2310 Size: 3 3 21 282 27 794 243 Fragments arranged by size: 794 332 285 282 243 94 82 66 30 27 21 15 9 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 263 1400 1929 2217 2310 Size: 263 1137 529 288 93 Fragments arranged by size: 1137 529 288 263 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 1567 1585 2310 Size: 584 983 18 725 Fragments arranged by size: 983 725 584 18 BsiEI CG_ry'CG Cuts at: 0 676 708 800 1059 1715 1778 2310 Size: 676 32 92 259 656 63 532 Fragments arranged by size: 676 656 532 259 92 63 32 BsiHKAI G_wGCw'C Cuts at: 0 356 880 1178 1487 1928 1961 2310 Size: 356 524 298 309 441 33 349 Fragments arranged by size: 524 441 356 349 309 298 33 BsmI GAATG_Cn' Cuts at: 0 441 2310 Size: 441 1869 BsmBI CGTCTCn'nnnn_ Cuts at: 0 695 2233 2310 Size: 695 1538 77 Fragments arranged by size: 1538 695 77 Bsp1286I G_dGCh'C Cuts at: 0 356 880 1148 1178 1267 1487 1928 1961 Size: 356 524 268 30 89 220 441 33 Cuts at: 1961 1995 2176 2290 2310 Size: 34 181 114 20 Fragments arranged by size: 524 441 356 268 220 181 114 89 34 33 30 20 BspHI T'CATG_A Cuts at: 0 2021 2310 Size: 2021 289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 755 1208 1589 1634 2310 Size: 755 453 381 45 676 Fragments arranged by size: 755 676 453 381 45 BsrBI CCG'CTC Cuts at: 0 492 549 564 576 687 2310 Size: 492 57 15 12 111 1623 Fragments arranged by size: 1623 492 111 57 15 12 BsrFI r'CCGG_y Cuts at: 0 934 1203 1642 2310 Size: 934 269 439 668 Fragments arranged by size: 934 668 439 269 BstAPI GCAn_nnn'nTGC Cuts at: 0 1188 2310 Size: 1188 1122 BstEII G'GTnAC_C Cuts at: 0 1719 2310 Size: 1719 591 BstXI CCAn_nnnn'nTGG Cuts at: 0 1779 2310 Size: 1779 531 BstYI r'GATC_y Cuts at: 0 1457 1595 1687 1881 2310 Size: 1457 138 92 194 429 Fragments arranged by size: 1457 429 194 138 92 Bsu36I CC'TnA_GG Cuts at: 0 2192 2310 Size: 2192 118 BtgI C'CryG_G Cuts at: 0 679 1772 1778 2282 2310 Size: 679 1093 6 504 28 Fragments arranged by size: 1093 679 504 28 6 ClaI AT'CG_AT Cuts at: 0 1856 2310 Size: 1856 454 EaeI y'GGCC_r Cuts at: 0 248 673 705 797 1775 2310 Size: 248 425 32 92 978 535 Fragments arranged by size: 978 535 425 248 92 32 EagI C'GGCC_G Cuts at: 0 673 705 797 1775 2310 Size: 673 32 92 978 535 Fragments arranged by size: 978 673 535 92 32 EarI CTCTTCn'nnn_ Cuts at: 0 198 412 719 836 851 905 911 2310 Size: 198 214 307 117 15 54 6 1399 Fragments arranged by size: 1399 307 214 198 117 54 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 571 2310 Size: 571 1739 EcoICRI GAG'CTC Cuts at: 0 1176 1926 2310 Size: 1176 750 384 Fragments arranged by size: 1176 750 384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 675 1351 1717 1935 2254 2280 2310 Size: 34 641 676 366 218 319 26 30 Fragments arranged by size: 676 641 366 319 218 34 30 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 1129 2195 2310 Size: 145 984 1066 115 Fragments arranged by size: 1066 984 145 115 EcoO109I rG'GnC_Cy Cuts at: 0 744 1889 2186 2273 2287 2310 Size: 744 1145 297 87 14 23 Fragments arranged by size: 1145 744 297 87 23 14 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1371 1965 2310 Size: 1371 594 345 Fragments arranged by size: 1371 594 345 HaeII r_GCGC'y Cuts at: 0 1124 1986 2066 2310 Size: 1124 862 80 244 Fragments arranged by size: 1124 862 244 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 736 768 793 825 940 972 1672 1704 Size: 736 32 25 32 115 32 700 32 Cuts at: 1704 1774 1806 2310 Size: 70 32 504 Fragments arranged by size: 736 700 504 115 70 32 32 32 32 32 25 KpnI G_GTAC'C Cuts at: 0 1734 2310 Size: 1734 576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 343 779 1646 2310 Size: 343 436 867 664 Fragments arranged by size: 867 664 436 343 MscI TGG'CCA Cuts at: 0 250 2310 Size: 250 2060 MslI CAynn'nnrTG Cuts at: 0 1468 1777 2310 Size: 1468 309 533 Fragments arranged by size: 1468 533 309 MspA1I CmG'CkG Cuts at: 0 528 681 814 1560 2217 2284 2310 Size: 528 153 133 746 657 67 26 Fragments arranged by size: 746 657 528 153 133 67 26 NaeI GCC'GGC Cuts at: 0 1205 1644 2310 Size: 1205 439 666 Fragments arranged by size: 1205 666 439 NgoMIV G'CCGG_C Cuts at: 0 1203 1642 2310 Size: 1203 439 668 Fragments arranged by size: 1203 668 439 NspI r_CATG'y Cuts at: 0 334 1788 2310 Size: 334 1454 522 Fragments arranged by size: 1454 522 334 PciI A'CATG_T Cuts at: 0 1784 2310 Size: 1784 526 PflMI CCAn_nnn'nTGG Cuts at: 0 653 1032 2310 Size: 653 379 1278 Fragments arranged by size: 1278 653 379 PfoI T'CCnGG_A Cuts at: 0 445 2310 Size: 445 1865 PmlI CAC'GTG Cuts at: 0 2135 2310 Size: 2135 175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1036 1068 2310 Size: 1036 32 1242 Fragments arranged by size: 1242 1036 32 PpuMI rG'GwC_Cy Cuts at: 0 744 1889 2310 Size: 744 1145 421 Fragments arranged by size: 1145 744 421 PspOMI G'GGCC_C Cuts at: 0 2286 2310 Size: 2286 24 PstI C_TGCA'G Cuts at: 0 1214 2310 Size: 1214 1096 PvuII CAG'CTG Cuts at: 0 1560 2217 2310 Size: 1560 657 93 Fragments arranged by size: 1560 657 93 SacI G_AGCT'C Cuts at: 0 1178 1928 2310 Size: 1178 750 382 Fragments arranged by size: 1178 750 382 SacII CC_GC'GG Cuts at: 0 682 2285 2310 Size: 682 1603 25 Fragments arranged by size: 1603 682 25 SanDI GG'GwC_CC Cuts at: 0 1889 2310 Size: 1889 421 SbfI CC_TGCA'GG Cuts at: 0 1214 2310 Size: 1214 1096 SfcI C'TryA_G Cuts at: 0 627 1210 1281 1917 2208 2310 Size: 627 583 71 636 291 102 Fragments arranged by size: 636 627 583 291 102 71 SmaI CCC'GGG Cuts at: 0 1842 2049 2310 Size: 1842 207 261 Fragments arranged by size: 1842 261 207 SmlI C'TyrA_G Cuts at: 0 142 1177 1393 1492 1612 1651 2077 2302 Size: 142 1035 216 99 120 39 426 225 Cuts at: 2302 2310 Size: 8 Fragments arranged by size: 1035 426 225 216 142 120 99 39 8 SphI G_CATG'C Cuts at: 0 334 2310 Size: 334 1976 StuI AGG'CCT Cuts at: 0 1702 2201 2310 Size: 1702 499 109 Fragments arranged by size: 1702 499 109 StyI C'CwwG_G Cuts at: 0 1760 2310 Size: 1760 550 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1073 1087 1648 2209 2310 Size: 1073 14 561 561 101 Fragments arranged by size: 1073 561 561 101 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 867 899 1225 1257 1881 1913 2310 Size: 867 32 326 32 624 32 397 Fragments arranged by size: 867 624 397 326 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1564 2310 Size: 1564 746 XmaI C'CCGG_G Cuts at: 0 1840 2047 2310 Size: 1840 207 263 Fragments arranged by size: 1840 263 207 XmnI GAAnn'nnTTC Cuts at: 0 47 287 2029 2310 Size: 47 240 1742 281 Fragments arranged by size: 1742 281 240 47 ZraI GAC'GTC Cuts at: 0 1619 2310 Size: 1619 691 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI SmaI SmlI SphI StuI StyI TaqII TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmrI BmtI BplI BsaWI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI