################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccI AclI AcyI AflII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BbrPI BciVI BclI BcnI BfrI BlnI BsaHI BsiEI BsiWI BsmBI BsmI BspDI BspHI BssHII Bst1107I BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KspI MluI NarI NciI NcoI NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1728 GGTACC G^GTACC AccII 1526 CGCG CG^CG AccIII 2361 TCCGGA T^CCGGA AhdI 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1728 GGTACC G^GTACC AspEI 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 2098 GCAGTACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 460 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 336 GCTCAGC GC^T[ACGT]AGC Bpu1102I 336 GCTCAGC GC^T[ACGT]AGC BsaAI 2948 TACGTG [CT]AC^GT[GA] BsaBI 1918 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2361 TCCGGA T^CCGGA BspEI 2361 TCCGGA T^CCGGA BspLU11I 1250 ACATGT A^CATGT BsrBI 334 CCGCTC CCGCTC(-3/-3) BsrFI 2216 GCCGGC [GA]^CCGG[CT] BstBI 74 TTCGAA TT^CGAA BstEII 768 GGTCACC G^GT[ACGT]ACC BstPI 768 GGTCACC G^GT[ACGT]ACC BstUI 1526 CGCG CG^CG CelII 336 GCTCAGC GC^T[ACGT]AGC CfoI 1353 GCGC GCG^C Cfr10I 2216 GCCGGC [GA]^CCGG[CT] DraI 609 TTTAAA TTT^AAA Eam1105I 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 768 GGTCACC G^GT[ACGT]ACC FauI 1865 CCCGC CCCGC(4/6) HaeII 1352 AGCGCC [GA]GCGC^[CT] HhaI 1353 GCGC GCG^C HinP1I 1353 GCGC G^CGC KpnI 1728 GGTACC GGTAC^C MamI 1918 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2361 TCCGGA T^CCGGA MvnI 1526 CGCG CG^CG NaeI 2216 GCCGGC GCC^GGC NgoMIV 2216 GCCGGC G^CCGGC NspV 74 TTCGAA TT^CGAA PflMI 542 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 2570 GAAGAGC GCTCTTC(1/4) SexAI 3062 ACCAGGT A^CC[AT]GGT SfuI 74 TTCGAA TT^CGAA SpeI 432 ACTAGT A^CTAGT Van91I 542 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2250 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AflIII 1250 ACATGT A^C[GA][CT]GT 1944 ACGTGT AocI 2701 CCTCAGG CC^T[ACGT]AGG 3313 CCTGAGG AvaI 1733 CTCGAG C^[CT]CG[GA]G 1787 CTCGAG BalI 99 TGGCCA TGG^CCA 2852 TGGCCA BamHI 1600 GGATCC G^GATCC 1782 GGATCC BbvCI 526 CCTCAGC CCTCAGC(-5/-2) 3199 GCTGAGG BglII 1689 AGATCT A^GATCT 2559 AGATCT BsaWI 897 ACCGGA [AT]^CCGG[AT] 2361 TCCGGA BsoBI 1733 CTCGAG C^[CT]CG[GA]G 1787 CTCGAG Bsp1407I 6 TGTACA T^GTACA 1300 TGTACA BsrDI 206 CATTGC GCAATG(2/0) 592 GCAATG BsrGI 6 TGTACA T^GTACA 1300 TGTACA BssSI 217 CTCGTG CACGAG(-5/-1) 1286 CTCGTG BstAPI 113 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 140 GCAAGGCTTGC Bsu36I 2701 CCTCAGG CC^T[ACGT]AGG 3313 CCTGAGG DsaI 694 CCACGG C^C[GA][CT]GG 2757 CCACGG Eco81I 2701 CCTCAGG CC^T[ACGT]AGG 3313 CCTGAGG EcoNI 3300 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3313 CCTGAGGGAGG EcoT14I 582 CCTTGG C^C[AT][AT]GG 1675 CCAAGG HincII 428 GTCAAC GT[CT]^[GA]AC 1391 GTCAAC HindII 428 GTCAAC GT[CT]^[GA]AC 1391 GTCAAC HindIII 406 AAGCTT A^AGCTT 605 AAGCTT MfeI 1013 CAATTG C^AATTG 2914 CAATTG MluNI 99 TGGCCA TGG^CCA 2852 TGGCCA MscI 99 TGGCCA TGG^CCA 2852 TGGCCA MunI 1013 CAATTG C^AATTG 2914 CAATTG NdeI 946 CATATG CA^TATG 2747 CATATG NspI 729 GCATGT [GA]CATG^[CT] 1250 ACATGT PaeR7I 1733 CTCGAG C^TCGAG 1787 CTCGAG PshAI 414 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1090 GACCCTAGTC PstI 211 CTGCAG CTGCA^G 2093 CTGCAG SfcI 211 CTGCAG C^T[GA][CT]AG 2093 CTGCAG SspBI 6 TGTACA T^GTACA 1300 TGTACA StyI 582 CCTTGG C^C[AT][AT]GG 1675 CCAAGG XcmI 2486 CCAAAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3007 CCACCTCCAAAATGG XhoI 1733 CTCGAG C^TCGAG 1787 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI