(Circular) (Six-base) MAPSORT of: pFN21AE4270.seq Check: 2348 from: 1 to: 8080 pFN21AE4270 8080 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4105 4105 Size: 8080 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5890 278 Size: 53 83 186 936 4354 2468 Fragments arranged by size: 4354 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5423 1937 Size: 3486 4594 Acc65I G'GTAC_C Cuts at: 3733 5401 3733 Size: 1668 6412 AclI AA'CG_TT Cuts at: 1563 6207 6580 1563 Size: 4644 373 3063 Fragments arranged by size: 4644 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3405 3710 4553 4807 6143 7527 7795 1632 Size: 1773 305 843 254 1336 1384 268 1917 Fragments arranged by size: 1917 1773 1384 1336 843 305 268 254 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7883 AflIII A'CryG_T Cuts at: 1203 3255 3949 6979 7956 1203 Size: 2052 694 3030 977 1327 Fragments arranged by size: 3030 2052 1327 977 694 AgeI A'CCGG_T Cuts at: 6911 6911 Size: 8080 AhdI GACnn_n'nnGTC Cuts at: 2911 6809 2911 Size: 3898 4182 AleI CACnn'nnGTG Cuts at: 1166 4824 1166 Size: 3658 4422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8080 AlwNI CAG_nnn'CTG Cuts at: 5823 7395 5823 Size: 1572 6508 ApaLI G'TGCA_C Cuts at: 6137 7293 6137 Size: 1156 6924 ApoI r'AATT_y Cuts at: 1493 2819 3338 4373 5389 5615 5767 7876 1493 Size: 1326 519 1035 1016 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 1035 1016 519 226 152 AseI AT'TA_AT Cuts at: 160 1032 6634 160 Size: 872 5602 1606 Fragments arranged by size: 5602 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8080 AvaI C'yCGr_G Cuts at: 1870 1943 3738 3792 5405 1870 Size: 73 1795 54 1613 4545 Fragments arranged by size: 4545 1795 1613 73 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3971 4004 4181 4214 3971 Size: 33 177 33 7837 Fragments arranged by size: 7837 177 33 33 BamHI G'GATC_C Cuts at: 3605 3787 5410 5777 3605 Size: 182 1623 367 5908 Fragments arranged by size: 5908 1623 367 182 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3733 4643 4702 5401 6856 Size: 325 217 627 1946 910 59 699 1455 Cuts at: 6856 618 Size: 1842 Fragments arranged by size: 1946 1842 1455 910 699 627 325 217 59 BanII G_rGCy'C Cuts at: 1391 2193 2511 4717 5399 1391 Size: 802 318 2206 682 4072 Fragments arranged by size: 4072 2206 802 682 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2283 2796 3379 4392 928 Size: 971 384 513 583 1013 4616 Fragments arranged by size: 4616 1013 971 583 513 384 BbvCI CC'TCA_GC Cuts at: 2532 5205 2532 Size: 2673 5407 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4092 4126 6271 6305 1903 Size: 34 50 34 2071 34 2145 34 3678 Fragments arranged by size: 3678 2145 2071 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5972 7182 5972 Size: 1210 6870 BclI T'GATC_A Cuts at: 1548 1548 Size: 8080 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2471 6691 136 Size: 107 122 71 631 605 799 4220 1525 Fragments arranged by size: 4220 1525 799 631 605 122 107 71 BglII A'GATC_T Cuts at: 3694 4564 8075 3694 Size: 870 3511 3699 Fragments arranged by size: 3699 3511 870 BlpI GC'TnA_GC Cuts at: 2342 5497 2342 Size: 3155 4925 Bme1580I G_kGCm'C Cuts at: 1790 6141 7297 1790 Size: 4351 1156 2573 Fragments arranged by size: 4351 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5361 5811 6769 Size: 416 53 83 399 24 3944 450 958 Cuts at: 6769 442 Size: 1753 Fragments arranged by size: 3944 1753 958 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8080 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2493 2493 Size: 8080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2967 3011 3057 6740 1239 Size: 1262 466 44 46 3683 2579 Fragments arranged by size: 3683 2579 1262 466 46 44 Bpu10I CC'TnA_GC Cuts at: 2232 2241 2532 5205 2232 Size: 9 291 2673 5107 Fragments arranged by size: 5107 2673 291 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2694 3400 5023 6205 7070 7368 2694 Size: 706 1623 1182 865 298 3406 Fragments arranged by size: 3406 1623 1182 865 706 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2925 3033 4611 6743 882 Size: 617 1426 108 1578 2132 2219 Fragments arranged by size: 2219 2132 1578 1426 617 108 BsaAI yAC'GTr Cuts at: 493 1204 4955 5871 493 Size: 711 3751 916 2702 Fragments arranged by size: 3751 2702 916 711 BsaBI GATnn'nnATC Cuts at: 3927 8074 3927 Size: 4147 3933 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5887 6269 6904 275 Size: 53 83 186 936 4354 382 635 1451 Fragments arranged by size: 4354 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2902 4366 6513 6911 7185 7332 7743 2902 Size: 1464 2147 398 274 147 411 3239 Fragments arranged by size: 3239 2147 1464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2687 2717 3924 3954 4336 Size: 30 165 30 1284 30 1207 30 382 Cuts at: 4336 4366 1178 Size: 30 4892 Fragments arranged by size: 4892 1284 1207 382 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2370 3102 3105 3294 3810 3900 4161 Size: 997 187 732 3 189 516 90 261 Cuts at: 4161 4221 4224 5340 1186 Size: 60 3 1116 3926 Fragments arranged by size: 3926 1116 997 732 516 261 189 187 90 60 3 3 BseYI C'CCAG_C Cuts at: 3043 3545 4239 4293 4400 4770 4947 7283 7779 Size: 502 694 54 107 370 177 2336 496 Cuts at: 7779 3043 Size: 3344 Fragments arranged by size: 3344 2336 694 502 496 370 177 107 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2888 3468 5048 1163 Size: 573 1152 580 1580 4195 Fragments arranged by size: 4195 1580 1152 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5530 6291 7319 1540 Size: 275 186 3529 761 1028 2301 Fragments arranged by size: 3529 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2233 2511 3601 4717 6141 6226 7297 2193 Size: 40 278 1090 1116 1424 85 1071 2976 Fragments arranged by size: 2976 1424 1116 1090 1071 278 85 40 BsmI GAATG_Cn' Cuts at: 5591 5684 5591 Size: 93 7987 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2233 2511 3601 4717 5399 6141 Size: 399 403 40 278 1090 1116 682 742 Cuts at: 6141 6226 7297 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1116 1090 1071 742 682 403 399 278 85 40 BspEI T'CCGG_A Cuts at: 4366 7743 4366 Size: 3377 4703 BspHI T'CATG_A Cuts at: 5969 7699 5969 Size: 1730 6350 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3627 3913 4105 5435 844 Size: 2783 286 192 1330 3489 Fragments arranged by size: 3489 2783 1330 286 192 BsrBI CCG'CTC Cuts at: 1394 1823 2341 5967 1394 Size: 429 518 3626 3507 Fragments arranged by size: 3626 3507 518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2210 2604 6575 6749 65 Size: 1183 962 394 3971 174 1396 Fragments arranged by size: 3971 1396 1183 962 394 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4221 6724 6911 1700 Size: 110 2411 2503 187 2869 Fragments arranged by size: 2869 2503 2411 187 110 BsrGI T'GTAC_A Cuts at: 96 2011 3305 96 Size: 1915 1294 4871 Fragments arranged by size: 4871 1915 1294 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8080 BssSI C'ACGA_G Cuts at: 2222 3291 6140 7152 2222 Size: 1069 2849 1012 3150 Fragments arranged by size: 3150 2849 1069 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2124 2151 794 Size: 1330 27 6723 Fragments arranged by size: 6723 1330 27 BstBI TT'CG_AA Cuts at: 2080 6884 2080 Size: 4804 3276 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 6491 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2588 3035 4955 2095 Size: 493 447 1920 5220 Fragments arranged by size: 5220 1920 493 447 BstYI r'GATC_y Cuts at: 1972 3454 3605 3694 3787 4564 5410 5777 6162 Size: 1482 151 89 93 777 846 367 385 Cuts at: 6162 6179 7631 7717 7729 8075 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1482 1452 846 777 385 367 346 151 93 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4707 5319 4707 Size: 612 7468 BtgI C'CryG_G Cuts at: 513 1065 2699 4762 513 Size: 552 1634 2063 3831 Fragments arranged by size: 3831 2063 1634 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4253 487 Size: 683 56 3027 4314 Fragments arranged by size: 4314 3027 683 56 BtsI GCAGTG_nn' Cuts at: 2353 3456 5236 5596 6390 6410 2353 Size: 1103 1780 360 794 20 4023 Fragments arranged by size: 4023 1780 1103 794 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8080 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8045 DraI TTT'AAA Cuts at: 2616 5384 2616 Size: 2768 5312 DraIII CAC_nnn'GTG Cuts at: 3002 3863 5071 3002 Size: 861 1208 6011 Fragments arranged by size: 6011 1208 861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7087 809 Size: 507 5771 1802 Fragments arranged by size: 5771 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 4018 4857 Size: 54 1425 50 275 18 274 1914 839 Cuts at: 4857 5527 6416 8045 8 Size: 670 889 1629 43 Fragments arranged by size: 1914 1629 1425 889 839 670 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5527 1537 Size: 275 3715 4090 Fragments arranged by size: 4090 3715 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2548 2800 4050 4570 5084 6010 1353 Size: 1195 252 1250 520 514 926 3423 Fragments arranged by size: 3423 1250 1195 926 520 514 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3795 6667 7041 7187 387 Size: 1064 138 2206 2872 374 146 1280 Fragments arranged by size: 2872 2206 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2509 4715 2191 Size: 318 2206 5556 Fragments arranged by size: 5556 2206 318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2967 3011 3057 3405 3710 4553 Size: 393 869 466 44 46 348 305 843 Cuts at: 4553 4807 6143 6740 7527 7795 1239 Size: 254 1336 597 787 268 1524 Fragments arranged by size: 1524 1336 869 843 787 597 466 393 348 305 268 254 46 44 EcoNI CCTnn'n_nnAGG Cuts at: 5309 5322 5309 Size: 13 8067 EcoO109I rG'GnC_Cy Cuts at: 2236 5209 5314 2236 Size: 2973 105 5002 Fragments arranged by size: 5002 2973 105 EcoRI G'AATT_C Cuts at: 5389 5767 5389 Size: 378 7702 FspI TGC'GCA Cuts at: 6586 6586 Size: 8080 HaeII r_GCGC'y Cuts at: 1678 3361 7227 1678 Size: 1683 3866 2531 Fragments arranged by size: 3866 2531 1683 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2777 2809 2897 Size: 32 135 32 452 32 721 32 88 Cuts at: 2897 2929 3721 3753 4144 4176 4826 4858 5281 Size: 32 792 32 391 32 650 32 423 Cuts at: 5281 5313 6721 6753 6795 6827 1373 Size: 32 1408 32 42 32 2626 Fragments arranged by size: 2626 1408 792 721 650 452 423 391 135 88 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2435 3398 5424 5670 669 Size: 988 281 497 963 2026 246 3079 Fragments arranged by size: 3079 2026 988 963 497 281 246 HindIII A'AGCT_T Cuts at: 748 2411 2610 748 Size: 1663 199 6218 Fragments arranged by size: 6218 1663 199 HpaI GTT'AAC Cuts at: 5670 5670 Size: 8080 KpnI G_GTAC'C Cuts at: 3737 5405 3737 Size: 1668 6412 MfeI C'AATT_G Cuts at: 3018 4919 5679 3018 Size: 1901 760 5419 Fragments arranged by size: 5419 1901 760 MluI A'CGCG_T Cuts at: 7956 7956 Size: 8080 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3904 4202 7194 7378 1305 Size: 2599 298 2992 184 2007 Fragments arranged by size: 2992 2599 2007 298 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 4859 10 Size: 54 1425 343 274 2753 3231 Fragments arranged by size: 3231 2753 1425 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 3256 3859 4014 4824 6038 6397 6556 Size: 648 2090 603 155 810 1214 359 159 Cuts at: 6556 518 Size: 2042 Fragments arranged by size: 2090 2042 1214 810 648 603 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2809 3133 3303 5496 6173 7321 7566 1606 Size: 1203 324 170 2193 677 1148 245 2120 Fragments arranged by size: 2193 2120 1203 1148 677 324 245 170 NaeI GCC'GGC Cuts at: 4223 4223 Size: 8080 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7528 NdeI CA'TA_TG Cuts at: 387 2952 4753 387 Size: 2565 1801 3714 Fragments arranged by size: 3714 2565 1801 NgoMIV G'CCGG_C Cuts at: 4221 4221 Size: 8080 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8080 NotI GC'GGCC_GC Cuts at: 5527 5527 Size: 8080 NspI r_CATG'y Cuts at: 1132 1229 2738 3259 5438 6983 1132 Size: 97 1509 521 2179 1545 2229 Fragments arranged by size: 2229 2179 1545 1509 521 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8080 PciI A'CATG_T Cuts at: 3255 6979 3255 Size: 3724 4356 PflMI CCAn_nnn'nTGG Cuts at: 1108 2553 1108 Size: 1445 6635 PfoI T'CCnGG_A Cuts at: 3601 3601 Size: 8080 PmeI GTTT'AAAC Cuts at: 5384 5384 Size: 8080 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6149 6181 7662 7694 6149 Size: 32 1481 32 6535 Fragments arranged by size: 6535 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 5209 5314 2236 Size: 2973 105 5002 Fragments arranged by size: 5002 2973 105 PshAI GACnn'nnGTC Cuts at: 1613 2423 3099 1613 Size: 810 676 6594 Fragments arranged by size: 6594 810 676 PsiI TTA'TAA Cuts at: 5650 5650 Size: 8080 PspXI vC'TCGA_Gb Cuts at: 3738 3792 3738 Size: 54 8026 PstI C_TGCA'G Cuts at: 830 2220 4102 5432 830 Size: 1390 1882 1330 3478 Fragments arranged by size: 3478 1882 1390 1330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8080 PvuII CAG'CTG Cuts at: 2809 3133 3303 2809 Size: 324 170 7586 Fragments arranged by size: 7586 324 170 SacI G_AGCT'C Cuts at: 2193 2511 4717 2193 Size: 318 2206 5556 Fragments arranged by size: 5556 2206 318 SalI G'TCGA_C Cuts at: 1936 5422 1936 Size: 3486 4594 SapI GCTCTTCn'nnn_ Cuts at: 4570 4570 Size: 8080 SbfI CC_TGCA'GG Cuts at: 5432 5432 Size: 8080 ScaI AGT'ACT Cuts at: 6328 8040 6328 Size: 1712 6368 SexAI A'CCwGG_T Cuts at: 5067 5067 Size: 8080 SfcI C'TryA_G Cuts at: 826 1045 2216 4098 5428 6563 7244 7435 826 Size: 219 1171 1882 1330 1135 681 191 1471 Fragments arranged by size: 1882 1471 1330 1171 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8080 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8080 SmaI CCC'GGG Cuts at: 1872 5407 1872 Size: 3535 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2709 3379 3738 3792 5038 6184 Size: 197 926 766 670 359 54 1246 1146 Cuts at: 6184 7085 7347 820 Size: 901 262 1553 Fragments arranged by size: 1553 1246 1146 926 901 766 670 359 262 197 54 SnaBI TAC'GTA Cuts at: 493 5871 493 Size: 5378 2702 SpeI A'CTAG_T Cuts at: 152 2437 152 Size: 2285 5795 SphI G_CATG'C Cuts at: 1132 5438 1132 Size: 4306 3774 SspI AAT'ATT Cuts at: 5 52 6004 5 Size: 47 5952 2081 Fragments arranged by size: 5952 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8080 StyI C'CwwG_G Cuts at: 513 1065 2587 3680 5519 513 Size: 552 1522 1093 1839 3074 Fragments arranged by size: 3074 1839 1522 1093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 4635 6107 6124 6277 6462 6907 Size: 1255 636 2419 1472 17 153 185 445 Cuts at: 6907 325 Size: 1498 Fragments arranged by size: 2419 1498 1472 1255 636 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2011 2328 3305 Size: 274 80 33 51 1443 34 317 977 Cuts at: 3305 6326 8038 96 Size: 3021 1712 138 Fragments arranged by size: 3021 1712 1443 977 317 274 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3924 3956 5105 5137 8040 8072 3924 Size: 32 1149 32 2903 32 3932 Fragments arranged by size: 3932 2903 1149 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8080 XbaI T'CTAG_A Cuts at: 4255 5416 4255 Size: 1161 6919 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4498 5019 4498 Size: 521 7559 XhoI C'TCGA_G Cuts at: 1943 3738 3792 1943 Size: 1795 54 6231 Fragments arranged by size: 6231 1795 54 XmaI C'CCGG_G Cuts at: 1870 5405 1870 Size: 3535 4545 XmnI GAAnn'nnTTC Cuts at: 2076 3339 3630 6209 2076 Size: 1263 291 2579 3947 Fragments arranged by size: 3947 2579 1263 291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5888 276 Size: 53 83 186 936 4354 2468 Fragments arranged by size: 4354 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI