(Linear) (Six-base) MAPSORT of: KIEE4270.seq Check: 517 from: 1 to: 3375 KIEE4270.orf With 182 enzymes: SgfI * July 3, 2012 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2100 3375 Size: 2100 1275 Acc65I G'GTAC_C Cuts at: 0 1728 3375 Size: 1728 1647 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1400 1705 2548 2802 3375 Size: 1400 305 843 254 573 Fragments arranged by size: 1400 843 573 305 254 AflIII A'CryG_T Cuts at: 0 1250 1944 3375 Size: 1250 694 1431 Fragments arranged by size: 1431 1250 694 AhdI GACnn_n'nnGTC Cuts at: 0 906 3375 Size: 906 2469 AleI CACnn'nnGTG Cuts at: 0 2819 3375 Size: 2819 556 ApoI r'AATT_y Cuts at: 0 814 1333 2368 3375 Size: 814 519 1035 1007 Fragments arranged by size: 1035 1007 814 519 AvaI C'yCGr_G Cuts at: 0 1733 1787 3375 Size: 1733 54 1588 Fragments arranged by size: 1733 1588 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1966 1999 2176 2209 3375 Size: 1966 33 177 33 1166 Fragments arranged by size: 1966 1166 177 33 33 BamHI G'GATC_C Cuts at: 0 1600 1782 3375 Size: 1600 182 1593 Fragments arranged by size: 1600 1593 182 BanI G'GyrC_C Cuts at: 0 1728 2638 2697 3375 Size: 1728 910 59 678 Fragments arranged by size: 1728 910 678 59 BanII G_rGCy'C Cuts at: 0 188 506 2712 3375 Size: 188 318 2206 663 Fragments arranged by size: 2206 663 318 188 BbsI GAAGACnn'nnnn_ Cuts at: 0 278 791 1374 2387 3375 Size: 278 513 583 1013 988 Fragments arranged by size: 1013 988 583 513 278 BbvCI CC'TCA_GC Cuts at: 0 527 3200 3375 Size: 527 2673 175 Fragments arranged by size: 2673 527 175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2087 2121 3375 Size: 2087 34 1254 Fragments arranged by size: 2087 1254 34 BglI GCCn_nnn'nGGC Cuts at: 0 466 3375 Size: 466 2909 BglII A'GATC_T Cuts at: 0 1689 2559 3375 Size: 1689 870 816 Fragments arranged by size: 1689 870 816 BlpI GC'TnA_GC Cuts at: 0 337 3375 Size: 337 3038 BmrI ACTGGGnnnn_n' Cuts at: 0 3356 3375 Size: 3356 19 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 488 3375 Size: 488 2887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 962 1006 1052 3375 Size: 496 466 44 46 2323 Fragments arranged by size: 2323 496 466 46 44 Bpu10I CC'TnA_GC Cuts at: 0 227 236 527 3200 3375 Size: 227 9 291 2673 175 Fragments arranged by size: 2673 291 227 175 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 689 1395 3018 3375 Size: 689 706 1623 357 Fragments arranged by size: 1623 706 689 357 BsaI GGTCTCn'nnnn_ Cuts at: 0 920 1028 2606 3375 Size: 920 108 1578 769 Fragments arranged by size: 1578 920 769 108 BsaAI yAC'GTr Cuts at: 0 2950 3375 Size: 2950 425 BsaBI GATnn'nnATC Cuts at: 0 1922 3375 Size: 1922 1453 BsaWI w'CCGG_w Cuts at: 0 897 2361 3375 Size: 897 1464 1014 Fragments arranged by size: 1464 1014 897 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 682 712 1919 1949 2331 2361 3375 Size: 682 30 1207 30 382 30 1014 Fragments arranged by size: 1207 1014 682 382 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 178 365 1097 1100 1289 1805 1895 2156 Size: 178 187 732 3 189 516 90 261 Cuts at: 2156 2216 2219 3335 3375 Size: 60 3 1116 40 Fragments arranged by size: 1116 732 516 261 189 187 178 90 60 40 3 3 BseYI C'CCAG_C Cuts at: 0 1038 1540 2234 2288 2395 2765 2942 3375 Size: 1038 502 694 54 107 370 177 433 Fragments arranged by size: 1038 694 502 433 370 177 107 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 883 1463 3043 3375 Size: 883 580 1580 332 Fragments arranged by size: 1580 883 580 332 BsiHKAI G_wGCw'C Cuts at: 0 188 228 506 1596 2712 3375 Size: 188 40 278 1090 1116 663 Fragments arranged by size: 1116 1090 663 278 188 40 Bsp1286I G_dGCh'C Cuts at: 0 188 228 506 1596 2712 3375 Size: 188 40 278 1090 1116 663 Fragments arranged by size: 1116 1090 663 278 188 40 BspEI T'CCGG_A Cuts at: 0 2361 3375 Size: 2361 1014 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1622 1908 2100 3375 Size: 1622 286 192 1275 Fragments arranged by size: 1622 1275 286 192 BsrBI CCG'CTC Cuts at: 0 336 3375 Size: 336 3039 BsrDI GCAATG_nn' Cuts at: 0 205 599 3375 Size: 205 394 2776 Fragments arranged by size: 2776 394 205 BsrFI r'CCGG_y Cuts at: 0 2216 3375 Size: 2216 1159 BsrGI T'GTAC_A Cuts at: 0 6 1300 3375 Size: 6 1294 2075 Fragments arranged by size: 2075 1294 6 BssSI C'ACGA_G Cuts at: 0 217 1286 3375 Size: 217 1069 2089 Fragments arranged by size: 2089 1069 217 BstAPI GCAn_nnn'nTGC Cuts at: 0 119 146 3375 Size: 119 27 3229 Fragments arranged by size: 3229 119 27 BstBI TT'CG_AA Cuts at: 0 75 3375 Size: 75 3300 BstEII G'GTnAC_C Cuts at: 0 768 3375 Size: 768 2607 BstXI CCAn_nnnn'nTGG Cuts at: 0 90 583 1030 2950 3375 Size: 90 493 447 1920 425 Fragments arranged by size: 1920 493 447 425 90 BstYI r'GATC_y Cuts at: 0 1449 1600 1689 1782 2559 3375 Size: 1449 151 89 93 777 816 Fragments arranged by size: 1449 816 777 151 93 89 Bsu36I CC'TnA_GG Cuts at: 0 2702 3314 3375 Size: 2702 612 61 Fragments arranged by size: 2702 612 61 BtgI C'CryG_G Cuts at: 0 694 2757 3375 Size: 694 2063 618 Fragments arranged by size: 2063 694 618 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2248 3375 Size: 2248 1127 BtsI GCAGTG_nn' Cuts at: 0 348 1451 3231 3375 Size: 348 1103 1780 144 Fragments arranged by size: 1780 1103 348 144 DraI TTT'AAA Cuts at: 0 611 3375 Size: 611 2764 DraIII CAC_nnn'GTG Cuts at: 0 997 1858 3066 3375 Size: 997 861 1208 309 Fragments arranged by size: 1208 997 861 309 EaeI y'GGCC_r Cuts at: 0 99 2013 2852 3375 Size: 99 1914 839 523 Fragments arranged by size: 1914 839 523 99 EarI CTCTTCn'nnn_ Cuts at: 0 543 795 2045 2565 3079 3375 Size: 543 252 1250 520 514 296 Fragments arranged by size: 1250 543 520 514 296 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1790 3375 Size: 1790 1585 EcoICRI GAG'CTC Cuts at: 0 186 504 2710 3375 Size: 186 318 2206 665 Fragments arranged by size: 2206 665 318 186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 962 1006 1052 1400 1705 2548 2802 Size: 496 466 44 46 348 305 843 254 Cuts at: 2802 3375 Size: 573 Fragments arranged by size: 843 573 496 466 348 305 254 46 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 3304 3317 3375 Size: 3304 13 58 Fragments arranged by size: 3304 58 13 EcoO109I rG'GnC_Cy Cuts at: 0 231 3204 3309 3375 Size: 231 2973 105 66 Fragments arranged by size: 2973 231 105 66 HaeII r_GCGC'y Cuts at: 0 1356 3375 Size: 1356 2019 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 19 51 772 804 892 924 1716 1748 Size: 19 32 721 32 88 32 792 32 Cuts at: 1748 2139 2171 2821 2853 3276 3308 3375 Size: 391 32 650 32 423 32 67 Fragments arranged by size: 792 721 650 423 391 88 67 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 0 430 1393 3375 Size: 430 963 1982 Fragments arranged by size: 1982 963 430 HindIII A'AGCT_T Cuts at: 0 406 605 3375 Size: 406 199 2770 Fragments arranged by size: 2770 406 199 KpnI G_GTAC'C Cuts at: 0 1732 3375 Size: 1732 1643 MfeI C'AATT_G Cuts at: 0 1013 2914 3375 Size: 1013 1901 461 Fragments arranged by size: 1901 1013 461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1899 2197 3375 Size: 1899 298 1178 Fragments arranged by size: 1899 1178 298 MscI TGG'CCA Cuts at: 0 101 2854 3375 Size: 101 2753 521 Fragments arranged by size: 2753 521 101 MslI CAynn'nnrTG Cuts at: 0 1251 1854 2009 2819 3375 Size: 1251 603 155 810 556 Fragments arranged by size: 1251 810 603 556 155 MspA1I CmG'CkG Cuts at: 0 804 1128 1298 3375 Size: 804 324 170 2077 Fragments arranged by size: 2077 804 324 170 NaeI GCC'GGC Cuts at: 0 2218 3375 Size: 2218 1157 NdeI CA'TA_TG Cuts at: 0 947 2748 3375 Size: 947 1801 627 Fragments arranged by size: 1801 947 627 NgoMIV G'CCGG_C Cuts at: 0 2216 3375 Size: 2216 1159 NspI r_CATG'y Cuts at: 0 733 1254 3375 Size: 733 521 2121 Fragments arranged by size: 2121 733 521 PciI A'CATG_T Cuts at: 0 1250 3375 Size: 1250 2125 PflMI CCAn_nnn'nTGG Cuts at: 0 548 3375 Size: 548 2827 PfoI T'CCnGG_A Cuts at: 0 1596 3375 Size: 1596 1779 PpuMI rG'GwC_Cy Cuts at: 0 231 3204 3309 3375 Size: 231 2973 105 66 Fragments arranged by size: 2973 231 105 66 PshAI GACnn'nnGTC Cuts at: 0 418 1094 3375 Size: 418 676 2281 Fragments arranged by size: 2281 676 418 PspXI vC'TCGA_Gb Cuts at: 0 1733 1787 3375 Size: 1733 54 1588 Fragments arranged by size: 1733 1588 54 PstI C_TGCA'G Cuts at: 0 215 2097 3375 Size: 215 1882 1278 Fragments arranged by size: 1882 1278 215 PvuII CAG'CTG Cuts at: 0 804 1128 1298 3375 Size: 804 324 170 2077 Fragments arranged by size: 2077 804 324 170 SacI G_AGCT'C Cuts at: 0 188 506 2712 3375 Size: 188 318 2206 663 Fragments arranged by size: 2206 663 318 188 SapI GCTCTTCn'nnn_ Cuts at: 0 2565 3375 Size: 2565 810 SexAI A'CCwGG_T Cuts at: 0 3062 3375 Size: 3062 313 SfcI C'TryA_G Cuts at: 0 211 2093 3375 Size: 211 1882 1282 Fragments arranged by size: 1882 1282 211 SmlI C'TyrA_G Cuts at: 0 704 1374 1733 1787 3033 3375 Size: 704 670 359 54 1246 342 Fragments arranged by size: 1246 704 670 359 342 54 SpeI A'CTAG_T Cuts at: 0 432 3375 Size: 432 2943 StyI C'CwwG_G Cuts at: 0 582 1675 3375 Size: 582 1093 1700 Fragments arranged by size: 1700 1093 582 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 211 2630 3375 Size: 211 2419 745 Fragments arranged by size: 2419 745 211 TatI w'GTAC_w Cuts at: 0 6 323 1300 3375 Size: 6 317 977 2075 Fragments arranged by size: 2075 977 317 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1919 1951 3100 3132 3375 Size: 1919 32 1149 32 243 Fragments arranged by size: 1919 1149 243 32 32 XbaI T'CTAG_A Cuts at: 0 2250 3375 Size: 2250 1125 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2493 3014 3375 Size: 2493 521 361 Fragments arranged by size: 2493 521 361 XhoI C'TCGA_G Cuts at: 0 1733 1787 3375 Size: 1733 54 1588 Fragments arranged by size: 1733 1588 54 XmnI GAAnn'nnTTC Cuts at: 0 71 1334 1625 3375 Size: 71 1263 291 1750 Fragments arranged by size: 1750 1263 291 71 Enzymes that do cut: AarI Acc65I AcuI AflIII AhdI AleI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PspXI PstI PvuII SacI SapI SexAI SfcI SmlI SpeI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BbeI BciVI BclI BfrBI Bme1580I BmgBI BmtI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BssHII BstZ17I ClaI CspCI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI MluI NarI NcoI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI Tth111I XmaI ZraI