Restriction map for
pFN21AE3471
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AspEI
AviII AvrII BalI BbrPI BclI
BfrI BlnI BsaBI BsaI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrGI ClaI CpoI Eam1105I
FbaI FseI FspI HpaI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI SfiI
SnaBI SphI Sse8387I SspBI SwaI
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 2633 GGTACC G^GTACC
AccI 2607 GTATAC GT^[AC][GT]AC
AseI 2772 ATTAAT AT^TAAT
AsnI 2772 ATTAAT AT^TAAT
Asp718I 2633 GGTACC G^GTACC
AspI 846 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1175 GGCGCC GGCGC^C
BcgI 2862 GCAATATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3805 TACGTG [CT]AC^GT[GA]
BsaWI 3498 TCCGGT [AT]^CCGG[AT]
BsmI 1254 GCATTC GAATGC(1/-1)
BspHI 3051 TCATGA T^CATGA
BspLU11I 1794 ACATGT A^CATGT
BsrDI 3872 GCAATG GCAATG(2/0)
BssHII 291 GCGCGC G^CGCGC
BssSI 1376 CACGAG CACGAG(-5/-1)
Bst1107I 2607 GTATAC GTA^TAC
BstBI 3276 TTCGAA TT^CGAA
BstEII 4346 GGTTACC G^GT[ACGT]ACC
BstPI 4346 GGTTACC G^GT[ACGT]ACC
BstXI 4275 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2607 GTATAC GTA^TAC
DraI 1593 TTTAAA TTT^AAA
DraIII 3230 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 846 GACTAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4270 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4346 GGTTACC G^GT[ACGT]ACC
EcoRV 2116 GATATC GAT^ATC
EcoT22I 4246 ATGCAT ATGCA^T
HincII 3378 GTTGAC GT[CT]^[GA]AC
HindII 3378 GTTGAC GT[CT]^[GA]AC
HindIII 1463 AAGCTT A^AGCTT
KasI 1175 GGCGCC G^GCGCC
KpnI 2633 GGTACC GGTAC^C
KspI 221 CCGCGG CCGC^GG
NarI 1175 GGCGCC GG^CGCC
NcoI 4110 CCATGG C^CATGG
NotI 764 GCGGCCGC GC^GGCCGC
NsiI 4246 ATGCAT ATGCA^T
NspV 3276 TTCGAA TT^CGAA
PflFI 846 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4278 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2772 ATTAAT AT^TAAT
RcaI 3051 TCATGA T^CATGA
SacI 447 GAGCTC GAGCT^C
SacII 221 CCGCGG CCGC^GG
SexAI 3231 ACCTGGT A^CC[AT]GGT
SfoI 1175 GGCGCC GGC^GCC
SfuI 3276 TTCGAA TT^CGAA
SgrAI 646 CACCGGCG C[GA]^CCGG[CT]G
SmaI 246 CCCGGG CCC^GGG
SmlI 1578 CTCAAG C^T[CT][GA]AG
SpeI 1504 ACTAGT A^CTAGT
SspI 1882 AATATT AAT^ATT
Tth111I 846 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 246 CCCGGG C^CCGGG
XmaI 246 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 314 GGCGTC G[GA]^CG[CT]C
1175 GGCGCC
AocI 919 CCTAAGG CC^T[ACGT]AGG
1002 CCTGAGG
Aor51HI 778 AGCGCT AGC^GCT
2954 AGCGCT
ApaI 249 GGGCCC GGGCC^C
322 GGGCCC
BamHI 2943 GGATCC G^GATCC
3838 GGATCC
BciVI 4297 GGATAC GTATCC(6/5)
2705 GTATCC
BlpI 3397 GCTCAGC GC^T[ACGT]AGC
3739 GCTCAGC
Bpu1102I 3397 GCTCAGC GC^T[ACGT]AGC
3739 GCTCAGC
BsaHI 314 GGCGTC G[GA]^CG[CT]C
1175 GGCGCC
BsgI 2958 CTGCAC GTGCAG(16/14)
3355 GTGCAG
BsrFI 647 ACCGGC [GA]^CCGG[CT]
3569 ACCGGC
BstAPI 138 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
462 GCAGGGGCTGC
Bsu36I 919 CCTAAGG CC^T[ACGT]AGG
1002 CCTGAGG
CelII 3397 GCTCAGC GC^T[ACGT]AGC
3739 GCTCAGC
Cfr10I 647 ACCGGC [GA]^CCGG[CT]
3569 ACCGGC
Eco47III 778 AGCGCT AGC^GCT
2954 AGCGCT
Eco81I 919 CCTAAGG CC^T[ACGT]AGG
1002 CCTGAGG
EcoRI 3293 GAATTC G^AATTC
3578 GAATTC
Hin1I 314 GGCGTC G[GA]^CG[CT]C
1175 GGCGCC
SapI 174 GCTCTTC GCTCTTC(1/4)
2974 GCTCTTC
ScaI 3961 AGTACT AGT^ACT
4165 AGTACT
XbaI 1285 TCTAGA T^CTAGA
3642 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI