(Linear) (Six-base) MAPSORT of: KIEE3471.seq Check: 6795 from: 1 to: 4356 KIEE3471.orf With 182 enzymes: SgfI * July 3, 2012 17:55 .. AccI GT'mk_AC Cuts at: 0 2608 4356 Size: 2608 1748 Acc65I G'GTAC_C Cuts at: 0 2633 4356 Size: 2633 1723 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 1354 1612 1871 2962 3800 4356 Size: 225 1129 258 259 1091 838 556 Fragments arranged by size: 1129 1091 838 556 259 258 225 AfeI AGC'GCT Cuts at: 0 780 2956 4356 Size: 780 2176 1400 Fragments arranged by size: 2176 1400 780 AflIII A'CryG_T Cuts at: 0 1142 1794 3806 4356 Size: 1142 652 2012 550 Fragments arranged by size: 2012 1142 652 550 AleI CACnn'nnGTG Cuts at: 0 3499 3805 4356 Size: 3499 306 551 Fragments arranged by size: 3499 551 306 AlwNI CAG_nnn'CTG Cuts at: 0 144 468 1002 1172 3631 4033 4356 Size: 144 324 534 170 2459 402 323 Fragments arranged by size: 2459 534 402 324 323 170 144 ApaI G_GGCC'C Cuts at: 0 253 326 4356 Size: 253 73 4030 Fragments arranged by size: 4030 253 73 ApoI r'AATT_y Cuts at: 0 2110 2261 2887 3293 3578 3930 4356 Size: 2110 151 626 406 285 352 426 Fragments arranged by size: 2110 626 426 406 352 285 151 AseI AT'TA_AT Cuts at: 0 2773 4356 Size: 2773 1583 AvaI C'yCGr_G Cuts at: 0 246 1074 3604 4356 Size: 246 828 2530 752 Fragments arranged by size: 2530 828 752 246 BamHI G'GATC_C Cuts at: 0 2943 3838 4356 Size: 2943 895 518 Fragments arranged by size: 2943 895 518 BanI G'GyrC_C Cuts at: 0 556 1175 2633 3228 4356 Size: 556 619 1458 595 1128 Fragments arranged by size: 1458 1128 619 595 556 BanII G_rGCy'C Cuts at: 0 253 326 451 1081 4356 Size: 253 73 125 630 3275 Fragments arranged by size: 3275 630 253 125 73 BbeI G_GCGC'C Cuts at: 0 1179 4356 Size: 1179 3177 BbsI GAAGACnn'nnnn_ Cuts at: 0 351 1924 3084 3266 4356 Size: 351 1573 1160 182 1090 Fragments arranged by size: 1573 1160 1090 351 182 BbvCI CC'TCA_GC Cuts at: 0 101 946 1439 3992 4356 Size: 101 845 493 2553 364 Fragments arranged by size: 2553 845 493 364 101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2851 2885 4356 Size: 2851 34 1471 Fragments arranged by size: 2851 1471 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2716 4291 4356 Size: 2716 1575 65 Fragments arranged by size: 2716 1575 65 BfrBI ATG'CAT Cuts at: 0 4248 4356 Size: 4248 108 BglI GCCn_nnn'nGGC Cuts at: 0 223 490 713 3349 4356 Size: 223 267 223 2636 1007 Fragments arranged by size: 2636 1007 267 223 223 BglII A'GATC_T Cuts at: 0 1261 3027 3639 4356 Size: 1261 1766 612 717 Fragments arranged by size: 1766 1261 717 612 BlpI GC'TnA_GC Cuts at: 0 3398 3740 4356 Size: 3398 342 616 Fragments arranged by size: 3398 616 342 Bme1580I G_kGCm'C Cuts at: 0 253 326 4356 Size: 253 73 4030 Fragments arranged by size: 4030 253 73 BmgBI CAC'GTC Cuts at: 0 1143 4356 Size: 1143 3213 BmrI ACTGGGnnnn_n' Cuts at: 0 447 964 2097 2665 4016 4356 Size: 447 517 1133 568 1351 340 Fragments arranged by size: 1351 1133 568 517 447 340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 2201 3467 4356 Size: 280 1921 1266 889 Fragments arranged by size: 1921 1266 889 280 Bpu10I CC'TnA_GC Cuts at: 0 43 101 946 1439 1958 3992 4356 Size: 43 58 845 493 519 2034 364 Fragments arranged by size: 2034 845 519 493 364 58 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1563 4356 Size: 1563 2793 BsaAI yAC'GTr Cuts at: 0 3807 4356 Size: 3807 549 BsaHI Gr'CG_yC Cuts at: 0 315 1176 4356 Size: 315 861 3180 Fragments arranged by size: 3180 861 315 BsaWI w'CCGG_w Cuts at: 0 3498 4356 Size: 3498 858 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 46 76 2174 2204 2784 2814 3014 3044 Size: 46 30 2098 30 580 30 200 30 Cuts at: 3044 4356 Size: 1312 Fragments arranged by size: 2098 1312 580 200 46 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 219 222 537 540 1020 1985 4356 Size: 219 3 315 3 480 965 2371 Fragments arranged by size: 2371 965 480 315 219 3 3 BseYI C'CCAG_C Cuts at: 0 24 47 371 474 560 1021 1102 4237 Size: 24 23 324 103 86 461 81 3135 Cuts at: 4237 4356 Size: 119 Fragments arranged by size: 3135 461 324 119 103 86 81 24 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2943 3376 4356 Size: 2943 433 980 Fragments arranged by size: 2943 980 433 BsiEI CG_ry'CG Cuts at: 0 200 768 2605 4356 Size: 200 568 1837 1751 Fragments arranged by size: 1837 1751 568 200 BsiHKAI G_wGCw'C Cuts at: 0 451 647 1208 4356 Size: 451 196 561 3148 Fragments arranged by size: 3148 561 451 196 BsmI GAATG_Cn' Cuts at: 0 1254 4356 Size: 1254 3102 Bsp1286I G_dGCh'C Cuts at: 0 253 326 451 647 1081 1208 4356 Size: 253 73 125 196 434 127 3148 Fragments arranged by size: 3148 434 253 196 127 125 73 BspHI T'CATG_A Cuts at: 0 3051 4356 Size: 3051 1305 BsrBI CCG'CTC Cuts at: 0 126 743 1097 1131 1181 4356 Size: 126 617 354 34 50 3175 Fragments arranged by size: 3175 617 354 126 50 34 BsrDI GCAATG_nn' Cuts at: 0 3879 4356 Size: 3879 477 BsrFI r'CCGG_y Cuts at: 0 647 3569 4356 Size: 647 2922 787 Fragments arranged by size: 2922 787 647 BssHII G'CGCG_C Cuts at: 0 291 4356 Size: 291 4065 BssSI C'ACGA_G Cuts at: 0 1376 4356 Size: 1376 2980 BstAPI GCAn_nnn'nTGC Cuts at: 0 144 468 4356 Size: 144 324 3888 Fragments arranged by size: 3888 324 144 BstBI TT'CG_AA Cuts at: 0 3277 4356 Size: 3277 1079 BstEII G'GTnAC_C Cuts at: 0 4346 4356 Size: 4346 10 BstXI CCAn_nnnn'nTGG Cuts at: 0 4282 4356 Size: 4282 74 BstYI r'GATC_y Cuts at: 0 1261 1288 2943 3027 3249 3542 3639 3838 Size: 1261 27 1655 84 222 293 97 199 Cuts at: 3838 4356 Size: 518 Fragments arranged by size: 1655 1261 518 293 222 199 97 84 27 BstZ17I GTA'TAC Cuts at: 0 2609 4356 Size: 2609 1747 Bsu36I CC'TnA_GG Cuts at: 0 920 1003 4356 Size: 920 83 3353 Fragments arranged by size: 3353 920 83 BtgI C'CryG_G Cuts at: 0 221 4110 4151 4356 Size: 221 3889 41 205 Fragments arranged by size: 3889 221 205 41 BtsI GCAGTG_nn' Cuts at: 0 621 2813 2960 4146 4356 Size: 621 2192 147 1186 210 Fragments arranged by size: 2192 1186 621 210 147 DraI TTT'AAA Cuts at: 0 1595 4356 Size: 1595 2761 DraIII CAC_nnn'GTG Cuts at: 0 3235 4356 Size: 3235 1121 DrdI GACnn_nn'nnGTC Cuts at: 0 852 4356 Size: 852 3504 EaeI y'GGCC_r Cuts at: 0 197 618 765 785 2602 4356 Size: 197 421 147 20 1817 1754 Fragments arranged by size: 1817 1754 421 197 147 20 EagI C'GGCC_G Cuts at: 0 197 765 2602 4356 Size: 197 568 1837 1754 Fragments arranged by size: 1837 1754 568 197 EarI CTCTTCn'nnn_ Cuts at: 0 181 1330 1631 2725 2981 3425 3849 3858 Size: 181 1149 301 1094 256 444 424 9 Cuts at: 3858 4356 Size: 498 Fragments arranged by size: 1149 1094 498 444 424 301 256 181 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 67 358 4356 Size: 67 291 3998 Fragments arranged by size: 3998 291 67 EcoICRI GAG'CTC Cuts at: 0 449 4356 Size: 449 3907 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 280 1354 1612 1871 2201 2962 3467 Size: 225 55 1074 258 259 330 761 505 Cuts at: 3467 3800 4356 Size: 333 556 Fragments arranged by size: 1074 761 556 505 333 330 259 258 225 55 EcoNI CCTnn'n_nnAGG Cuts at: 0 4274 4356 Size: 4274 82 EcoO109I rG'GnC_Cy Cuts at: 0 39 250 323 590 989 4279 4356 Size: 39 211 73 267 399 3290 77 Fragments arranged by size: 3290 399 267 211 77 73 39 EcoRI G'AATT_C Cuts at: 0 3293 3578 4356 Size: 3293 285 778 Fragments arranged by size: 3293 778 285 EcoRV GAT'ATC Cuts at: 0 2118 4356 Size: 2118 2238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2247 2511 3849 4356 Size: 2247 264 1338 507 Fragments arranged by size: 2247 1338 507 264 HaeII r_GCGC'y Cuts at: 0 25 636 782 1179 2958 4356 Size: 25 611 146 397 1779 1398 Fragments arranged by size: 1779 1398 611 397 146 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 433 465 808 840 1690 1722 2784 2816 Size: 433 32 343 32 850 32 1062 32 Cuts at: 2816 3771 3803 3928 3960 4155 4187 4356 Size: 955 32 125 32 195 32 169 Fragments arranged by size: 1062 955 850 433 343 195 169 125 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 3380 4356 Size: 3380 976 HindIII A'AGCT_T Cuts at: 0 1463 4356 Size: 1463 2893 KasI G'GCGC_C Cuts at: 0 1175 4356 Size: 1175 3181 KpnI G_GTAC'C Cuts at: 0 2637 4356 Size: 2637 1719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 44 560 1671 4356 Size: 44 516 1111 2685 Fragments arranged by size: 2685 1111 516 44 MslI CAynn'nnrTG Cuts at: 0 2426 3499 3805 3980 4356 Size: 2426 1073 306 175 376 Fragments arranged by size: 2426 1073 376 306 175 MspA1I CmG'CkG Cuts at: 0 114 223 426 530 564 713 761 1172 Size: 114 109 203 104 34 149 48 411 Cuts at: 1172 1351 1950 2908 3201 3732 4356 Size: 179 599 958 293 531 624 Fragments arranged by size: 958 624 599 531 411 293 203 179 149 114 109 104 48 34 NarI GG'CG_CC Cuts at: 0 1176 4356 Size: 1176 3180 NcoI C'CATG_G Cuts at: 0 4110 4356 Size: 4110 246 NotI GC'GGCC_GC Cuts at: 0 765 4356 Size: 765 3591 NsiI A_TGCA'T Cuts at: 0 4250 4356 Size: 4250 106 NspI r_CATG'y Cuts at: 0 1567 1798 2934 3670 4356 Size: 1567 231 1136 736 686 Fragments arranged by size: 1567 1136 736 686 231 PasI CC'CwG_GG Cuts at: 0 3177 4356 Size: 3177 1179 PciI A'CATG_T Cuts at: 0 1794 4356 Size: 1794 2562 PpuMI rG'GwC_Cy Cuts at: 0 4279 4356 Size: 4279 77 PspOMI G'GGCC_C Cuts at: 0 249 322 4356 Size: 249 73 4034 Fragments arranged by size: 4034 249 73 PstI C_TGCA'G Cuts at: 0 134 1422 2808 2907 4356 Size: 134 1288 1386 99 1449 Fragments arranged by size: 1449 1386 1288 134 99 PvuII CAG'CTG Cuts at: 0 1172 1351 2908 3201 3732 4356 Size: 1172 179 1557 293 531 624 Fragments arranged by size: 1557 1172 624 531 293 179 SacI G_AGCT'C Cuts at: 0 451 4356 Size: 451 3905 SacII CC_GC'GG Cuts at: 0 224 4356 Size: 224 4132 SapI GCTCTTCn'nnn_ Cuts at: 0 181 2981 4356 Size: 181 2800 1375 Fragments arranged by size: 2800 1375 181 ScaI AGT'ACT Cuts at: 0 3963 4167 4356 Size: 3963 204 189 Fragments arranged by size: 3963 204 189 SexAI A'CCwGG_T Cuts at: 0 3231 4356 Size: 3231 1125 SfcI C'TryA_G Cuts at: 0 130 927 1083 1107 1158 1194 1418 2804 Size: 130 797 156 24 51 36 224 1386 Cuts at: 2804 2903 3957 4356 Size: 99 1054 399 Fragments arranged by size: 1386 1054 797 399 224 156 130 99 51 36 24 SfoI GGC'GCC Cuts at: 0 1177 4356 Size: 1177 3179 SgrAI Cr'CCGG_yG Cuts at: 0 647 4356 Size: 647 3709 SmaI CCC'GGG Cuts at: 0 248 4356 Size: 248 4108 SmlI C'TyrA_G Cuts at: 0 1578 4356 Size: 1578 2778 SpeI A'CTAG_T Cuts at: 0 1504 4356 Size: 1504 2852 SrfI GCCC'GGGC Cuts at: 0 248 4356 Size: 248 4108 SspI AAT'ATT Cuts at: 0 1884 4356 Size: 1884 2472 StuI AGG'CCT Cuts at: 0 682 895 925 2037 4356 Size: 682 213 30 1112 2319 Fragments arranged by size: 2319 1112 682 213 30 StyI C'CwwG_G Cuts at: 0 857 890 1451 1487 1778 2709 2939 4110 Size: 857 33 561 36 291 931 230 1171 Cuts at: 4110 4275 4356 Size: 165 81 Fragments arranged by size: 1171 931 857 561 291 230 165 81 36 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 574 928 4356 Size: 574 354 3428 Fragments arranged by size: 3428 574 354 TatI w'GTAC_w Cuts at: 0 3961 4165 4356 Size: 3961 204 191 Fragments arranged by size: 3961 204 191 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3014 3046 4356 Size: 3014 32 1310 Fragments arranged by size: 3014 1310 32 Tth111I GACn'n_nGTC Cuts at: 0 849 4356 Size: 849 3507 XbaI T'CTAG_A Cuts at: 0 1285 3642 4356 Size: 1285 2357 714 Fragments arranged by size: 2357 1285 714 XmaI C'CCGG_G Cuts at: 0 246 4356 Size: 246 4110 XmnI GAAnn'nnTTC Cuts at: 0 1884 2231 3891 4356 Size: 1884 347 1660 465 Fragments arranged by size: 1884 1660 465 347 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI NotI NsiI NspI PasI PciI PpuMI PspOMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BclI BmtI BplI BsaI BsaBI BsiWI BsmBI BspEI BspMI BsrGI BtgZI ClaI CspCI FseI FspI FspAI HpaI MfeI MluI MscI NaeI NdeI NgoMIV NheI NruI PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI SgfI SnaBI SphI SwaI XcmI XhoI ZraI