################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BbrPI BbsI BclI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 449 GGTACC G^GTACC AflIII 391 ACATGT A^C[GA][CT]GT Alw44I 1216 GTGCAC G^TGCAC ApaLI 1216 GTGCAC G^TGCAC Asp718I 449 GGTACC G^GTACC AspI 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2882 CCCGGG C^[CT]CG[GA]G BalI 725 TGGCCA TGG^CCA BamHI 635 GGATCC G^GATCC BbeI 2261 GGCGCC GGCGC^C BbvCI 2122 CCTCAGC CCTCAGC(-5/-2) BciVI 168 GGATAC GTATCC(6/5) BglII 720 AGATCT A^GATCT BsaAI 2225 TACGTG [CT]AC^GT[GA] BsgI 2840 CTGCAC GTGCAG(16/14) BsoBI 2882 CCCGGG C^[CT]CG[GA]G BspHI 279 TCATGA T^CATGA BspLU11I 391 ACATGT A^CATGT BssHII 1522 GCGCGC G^CGCGC BssSI 1900 CTCGTG CACGAG(-5/-1) EagI 1493 CGGCCG C^GGCCG EclXI 1493 CGGCCG C^GGCCG Eco52I 1493 CGGCCG C^GGCCG HgaI 844 GACGC GACGC(5/10) HincII 828 GTTAAC GT[CT]^[GA]AC HindII 828 GTTAAC GT[CT]^[GA]AC HindIII 922 AAGCTT A^AGCTT HpaI 828 GTTAAC GTT^AAC KasI 2261 GGCGCC G^GCGCC KpnI 449 GGTACC GGTAC^C MluNI 725 TGGCCA TGG^CCA MscI 725 TGGCCA TGG^CCA MslI 255 CACTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2261 GGCGCC GG^CGCC NheI 459 GCTAGC G^CTAGC PflFI 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 390 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 279 TCATGA T^CATGA SapI 1764 GAAGAGC GCTCTTC(1/4) SbfI 2364 CCTGCAGG CCTGCA^GG SfiI 1944 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2261 GGCGCC GGC^GCC SmaI 2882 CCCGGG CCC^GGG Sse8387I 2364 CCTGCAGG CCTGCA^GG Tth111I 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 357 CCAACAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2882 CCCGGG C^CCGGG XmaI 2882 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1523 CGCG CG^CG 1573 CGCG AcyI 844 GACGCC G[GA]^CG[CT]C 2261 GGCGCC BcgI 1669 GCACTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2856 GCAGCTGCTTCG BsaHI 844 GACGCC G[GA]^CG[CT]C 2261 GGCGCC BsmBI 842 GAGACG CGTCTC(1/5) 1270 GAGACG BsmI 574 GAATGC GAATGC(1/-1) 2915 GCATTC BsrDI 2502 CATTGC GCAATG(2/0) 881 GCAATG BstUI 1523 CGCG CG^CG 1573 CGCG BstXI 2466 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2727 CCAGGAACTTGG EarI 916 GAAGAG CTCTTC(1/4) 1764 GAAGAG Hin1I 844 GACGCC G[GA]^CG[CT]C 2261 GGCGCC Ksp632I 916 GAAGAG CTCTTC(1/4) 1764 GAAGAG MaeII 1688 ACGT A^CGT 2226 ACGT MseI 829 TTAA T^TAA 962 TTAA MvnI 1523 CGCG CG^CG 1573 CGCG NaeI 2258 GCCGGC GCC^GGC 2779 GCCGGC NgoMIV 2258 GCCGGC G^CCGGC 2779 GCCGGC PflMI 1803 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1896 CCAGCTCGTGG SmlI 1440 CTTGAG C^T[CT][GA]AG 1597 CTCAAG SphI 687 GCATGC GCATG^C 953 GCATGC Tru9I 829 TTAA T^TAA 962 TTAA Tsp45I 937 GTGAC ^GT[GC]AC 2828 GTGAC Van91I 1803 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1896 CCAGCTCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp509I TspRI XhoII