(Circular) (Six-base) MAPSORT of: pFN21AB8549.seq Check: 1431 from: 1 to: 7675 pFN21AB8549 7675 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3208 3390 3831 4287 3208 Size: 182 441 456 6596 Fragments arranged by size: 6596 456 441 182 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5485 278 Size: 53 83 186 936 3949 2468 Fragments arranged by size: 3949 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5018 1937 Size: 3081 4594 Acc65I G'GTAC_C Cuts at: 2454 4996 2454 Size: 2542 5133 AclI AA'CG_TT Cuts at: 1563 5802 6175 1563 Size: 4239 373 3063 Fragments arranged by size: 4239 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 2742 2918 3770 3788 4262 4454 4559 Size: 824 286 176 852 18 474 192 105 Cuts at: 4559 4698 5738 7122 7390 1632 Size: 139 1040 1384 268 1917 Fragments arranged by size: 1917 1384 1040 852 824 474 286 268 192 176 139 105 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7478 AflIII A'CryG_T Cuts at: 1203 2396 6574 7551 1203 Size: 1193 4178 977 1327 Fragments arranged by size: 4178 1327 1193 977 AgeI A'CCGG_T Cuts at: 6506 6506 Size: 7675 AhdI GACnn_n'nnGTC Cuts at: 6404 6404 Size: 7675 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7675 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7675 AlwNI CAG_nnn'CTG Cuts at: 3271 3814 3853 3889 4630 5418 6990 3271 Size: 543 39 36 741 788 1572 3956 Fragments arranged by size: 3956 1572 788 741 543 39 36 ApaI G_GGCC'C Cuts at: 2417 3342 4030 4369 4723 2417 Size: 925 688 339 354 5369 Fragments arranged by size: 5369 925 688 354 339 ApaLI G'TGCA_C Cuts at: 3221 5732 6888 3221 Size: 2511 1156 4008 Fragments arranged by size: 4008 2511 1156 ApoI r'AATT_y Cuts at: 1493 4984 5210 5362 7471 1493 Size: 3491 226 152 2109 1697 Fragments arranged by size: 3491 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6229 160 Size: 872 5197 1606 Fragments arranged by size: 5197 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7675 AvaI C'yCGr_G Cuts at: 1870 1943 4887 5000 1870 Size: 73 2944 113 4545 Fragments arranged by size: 4545 2944 113 73 BamHI G'GATC_C Cuts at: 2640 5005 5372 2640 Size: 2365 367 4943 Fragments arranged by size: 4943 2365 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2454 4266 4548 4996 Size: 325 217 627 462 205 1812 282 448 Cuts at: 4996 6451 618 Size: 1455 1842 Fragments arranged by size: 1842 1812 1455 627 462 448 325 282 217 205 BanII G_rGCy'C Cuts at: 1391 2417 2602 2782 3330 3342 3507 3603 4030 Size: 1026 185 180 548 12 165 96 427 Cuts at: 4030 4369 4413 4710 4723 4994 1391 Size: 339 44 297 13 271 4072 Fragments arranged by size: 4072 1026 548 427 339 297 271 185 180 165 96 44 13 12 BbeI G_GCGC'C Cuts at: 4270 4270 Size: 7675 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6704 BbvCI CC'TCA_GC Cuts at: 4128 4128 Size: 7675 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3663 3697 4850 4884 5866 5900 1903 Size: 34 1726 34 1153 34 982 34 3678 Fragments arranged by size: 3678 1726 1153 982 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2167 5567 6777 2167 Size: 3400 1210 3065 Fragments arranged by size: 3400 3065 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7675 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3956 4178 4785 Size: 107 122 71 631 605 2284 222 607 Cuts at: 4785 6286 136 Size: 1501 1525 Fragments arranged by size: 2284 1525 1501 631 607 605 222 122 107 71 BglII A'GATC_T Cuts at: 2725 7670 2725 Size: 4945 2730 BlpI GC'TnA_GC Cuts at: 3375 3618 3740 5092 3375 Size: 243 122 1352 5958 Fragments arranged by size: 5958 1352 243 122 Bme1580I G_kGCm'C Cuts at: 1790 2417 3088 3225 3342 4030 4369 4723 5736 Size: 627 671 137 117 688 339 354 1013 Cuts at: 5736 6892 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1013 688 671 627 354 339 137 117 BmgBI CAC'GTC Cuts at: 1318 3694 1318 Size: 2376 5299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4024 5406 6364 Size: 416 53 83 399 24 2607 1382 958 Cuts at: 6364 442 Size: 1753 Fragments arranged by size: 2607 1753 1382 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2468 1056 Size: 1412 6263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 7675 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2467 2882 3293 3416 3503 3509 3563 Size: 1226 2 415 411 123 87 6 54 Cuts at: 3563 4178 4500 4637 6335 1239 Size: 615 322 137 1698 2579 Fragments arranged by size: 2579 1698 1226 615 415 411 322 137 123 87 54 6 2 Bpu10I CC'TnA_GC Cuts at: 3933 4128 3933 Size: 195 7480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3466 3587 5800 6665 6963 3466 Size: 121 2213 865 298 4178 Fragments arranged by size: 4178 2213 865 298 121 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3539 3798 4349 6338 882 Size: 617 2040 259 551 1989 2219 Fragments arranged by size: 2219 2040 1989 617 551 259 BsaAI yAC'GTr Cuts at: 493 1204 4232 5466 493 Size: 711 3028 1234 2702 Fragments arranged by size: 3028 2702 1234 711 BsaBI GATnn'nnATC Cuts at: 7669 7669 Size: 7675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2850 4267 5482 5864 Size: 53 83 186 936 1317 1417 1215 382 Cuts at: 5864 6499 275 Size: 635 1451 Fragments arranged by size: 1451 1417 1317 1215 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6108 6506 6780 6927 7338 6108 Size: 398 274 147 411 6445 Fragments arranged by size: 6445 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 1178 Size: 30 165 30 1035 30 6385 Fragments arranged by size: 6385 1035 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3092 3251 3485 4094 4097 4100 4109 4112 Size: 1906 159 234 609 3 3 9 3 Cuts at: 4112 4115 4118 4121 4124 4226 4229 4577 4610 Size: 3 3 3 3 102 3 348 33 Cuts at: 4610 1186 Size: 4251 Fragments arranged by size: 4251 1906 609 348 234 159 102 33 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3331 3706 3718 3900 4005 4318 4723 6878 7374 Size: 375 12 182 105 313 405 2155 496 Cuts at: 7374 3331 Size: 3632 Fragments arranged by size: 3632 2155 496 405 375 313 182 105 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4830 1163 Size: 573 3094 4008 Fragments arranged by size: 4008 3094 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3501 3562 4654 5125 5886 6914 Size: 275 186 1500 61 1092 471 761 1028 Cuts at: 6914 1540 Size: 2301 Fragments arranged by size: 2301 1500 1092 1028 761 471 275 186 61 BsiHKAI G_wGCw'C Cuts at: 3225 3381 3507 3603 4413 5736 5821 6892 3225 Size: 156 126 96 810 1323 85 1071 4008 Fragments arranged by size: 4008 1323 1071 810 156 126 96 85 BsmI GAATG_Cn' Cuts at: 2585 4920 5186 5279 2585 Size: 2335 266 93 4981 Fragments arranged by size: 4981 2335 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2841 3269 2841 Size: 428 7247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 2602 2782 3088 3225 3330 3342 Size: 399 627 185 180 306 137 105 12 Cuts at: 3342 3381 3507 3603 4030 4369 4413 4710 4723 Size: 39 126 96 427 339 44 297 13 Cuts at: 4723 4994 5736 5821 6892 1391 Size: 271 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 627 427 399 339 306 297 271 185 180 137 126 105 96 85 44 39 13 12 BspEI T'CCGG_A Cuts at: 7338 7338 Size: 7675 BspHI T'CATG_A Cuts at: 2284 5564 7294 2284 Size: 3280 1730 2665 Fragments arranged by size: 3280 2665 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3100 3208 3390 3831 4287 5030 844 Size: 2256 108 182 441 456 743 3489 Fragments arranged by size: 3489 2256 743 456 441 182 108 BsrBI CCG'CTC Cuts at: 1394 1823 2776 3082 3493 3630 4546 5562 1394 Size: 429 953 306 411 137 916 1016 3507 Fragments arranged by size: 3507 1016 953 916 429 411 306 137 BsrDI GCAATG_nn' Cuts at: 65 1248 2893 4506 6170 6344 65 Size: 1183 1645 1613 1664 174 1396 Fragments arranged by size: 1664 1645 1613 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2451 3030 4263 4784 6319 6506 1700 Size: 110 641 579 1233 521 1535 187 2869 Fragments arranged by size: 2869 1535 1233 641 579 521 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7675 BssHII G'CGCG_C Cuts at: 1925 3527 1925 Size: 1602 6073 BssSI C'ACGA_G Cuts at: 3905 5735 6747 3905 Size: 1830 1012 4833 Fragments arranged by size: 4833 1830 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2426 3199 4255 4858 4917 794 Size: 1632 773 1056 603 59 3552 Fragments arranged by size: 3552 1632 1056 773 603 59 BstBI TT'CG_AA Cuts at: 6479 6479 Size: 7675 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7675 BstXI CCAn_nnnn'nTGG Cuts at: 4478 4739 4478 Size: 261 7414 BstYI r'GATC_y Cuts at: 1972 2440 2640 2725 5005 5372 5757 5774 7226 Size: 468 200 85 2280 367 385 17 1452 Cuts at: 7226 7312 7324 7670 1972 Size: 86 12 346 1977 Fragments arranged by size: 2280 1977 1452 468 385 367 346 200 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2169 2603 2855 4305 2169 Size: 434 252 1450 5539 Fragments arranged by size: 5539 1450 434 252 BtgI C'CryG_G Cuts at: 513 1065 3072 4173 4380 513 Size: 552 2007 1101 207 3808 Fragments arranged by size: 3808 2007 1101 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4445 487 Size: 683 56 3219 3717 Fragments arranged by size: 3717 3219 683 56 BtsI GCAGTG_nn' Cuts at: 3674 4539 4795 4842 5191 5985 6005 3674 Size: 865 256 47 349 794 20 5344 Fragments arranged by size: 5344 865 794 349 256 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7675 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7640 DraI TTT'AAA Cuts at: 4979 4979 Size: 7675 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6682 809 Size: 507 5366 1802 Fragments arranged by size: 5366 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2730 3498 Size: 54 1425 50 275 18 204 696 768 Cuts at: 3498 3837 3948 5122 6011 7640 8 Size: 339 111 1174 889 1629 43 Fragments arranged by size: 1629 1425 1174 889 768 696 339 275 204 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3498 5122 1537 Size: 275 1686 1624 4090 Fragments arranged by size: 4090 1686 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2916 3764 5605 1353 Size: 1563 848 1841 3423 Fragments arranged by size: 3423 1841 1563 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3536 6262 6636 6782 387 Size: 1064 138 1947 2726 374 146 1280 Fragments arranged by size: 2726 1947 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3505 3601 4411 3505 Size: 96 810 6769 Fragments arranged by size: 6769 810 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2465 2467 2742 2882 2918 3293 Size: 393 824 9 2 275 140 36 375 Cuts at: 3293 3416 3503 3509 3563 3770 3788 4178 4262 Size: 123 87 6 54 207 18 390 84 Cuts at: 4262 4454 4500 4559 4637 4698 5738 6335 7122 Size: 192 46 59 78 61 1040 597 787 Cuts at: 7122 7390 1239 Size: 268 1524 Fragments arranged by size: 1524 1040 824 787 597 393 390 375 275 268 207 192 140 123 87 84 78 61 59 54 46 36 18 9 6 2 EcoNI CCTnn'n_nnAGG Cuts at: 3107 3386 4056 4803 3107 Size: 279 670 747 5979 Fragments arranged by size: 5979 747 670 279 EcoO109I rG'GnC_Cy Cuts at: 2413 2414 2893 2935 3338 3339 4153 4366 4719 Size: 1 479 42 403 1 814 213 353 Cuts at: 4719 4720 2413 Size: 1 5368 Fragments arranged by size: 5368 814 479 403 353 213 42 1 1 1 EcoRI G'AATT_C Cuts at: 4984 5362 4984 Size: 378 7297 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2945 2945 Size: 7675 FspI TGC'GCA Cuts at: 6181 6181 Size: 7675 HaeII r_GCGC'y Cuts at: 1678 3183 3991 4270 4897 6822 1678 Size: 1505 808 279 627 1925 2531 Fragments arranged by size: 2531 1925 1505 808 627 279 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 6316 6348 6390 Size: 32 135 32 475 32 4237 32 42 Cuts at: 6390 6422 1373 Size: 32 2626 Fragments arranged by size: 4237 2626 475 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 5019 5265 669 Size: 988 281 897 2184 246 3079 Fragments arranged by size: 3079 2184 988 897 281 246 HindIII A'AGCT_T Cuts at: 748 2927 748 Size: 2179 5496 HpaI GTT'AAC Cuts at: 2835 5265 2835 Size: 2430 5245 KasI G'GCGC_C Cuts at: 4266 4266 Size: 7675 KpnI G_GTAC'C Cuts at: 2458 5000 2458 Size: 2542 5133 MfeI C'AATT_G Cuts at: 5274 5274 Size: 7675 MluI A'CGCG_T Cuts at: 7551 7551 Size: 7675 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2541 2751 3663 6789 6973 1305 Size: 684 552 210 912 3126 184 2007 Fragments arranged by size: 3126 2007 912 684 552 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2732 10 Size: 54 1425 343 900 4953 Fragments arranged by size: 4953 1425 900 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 5633 5992 6151 518 Size: 648 1098 3369 359 159 2042 Fragments arranged by size: 3369 2042 1098 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3091 3613 3853 3916 4196 4240 4685 4864 Size: 1485 522 240 63 280 44 445 179 Cuts at: 4864 5091 5768 6916 7161 1606 Size: 227 677 1148 245 2120 Fragments arranged by size: 2120 1485 1148 677 522 445 280 245 240 227 179 63 44 NaeI GCC'GGC Cuts at: 4265 4786 4265 Size: 521 7154 NarI GG'CG_CC Cuts at: 4267 4267 Size: 7675 NcoI C'CATG_G Cuts at: 513 1065 3072 4173 4380 513 Size: 552 2007 1101 207 3808 Fragments arranged by size: 3808 2007 1101 552 207 NdeI CA'TA_TG Cuts at: 387 387 Size: 7675 NgoMIV G'CCGG_C Cuts at: 4263 4784 4263 Size: 521 7154 NheI G'CTAG_C Cuts at: 1052 2464 1052 Size: 1412 6263 NotI GC'GGCC_GC Cuts at: 5122 5122 Size: 7675 NspI r_CATG'y Cuts at: 1132 1229 2400 2696 2962 5033 6578 1132 Size: 97 1171 296 266 2071 1545 2229 Fragments arranged by size: 2229 2071 1545 1171 296 266 97 PasI CC'CwG_GG Cuts at: 1348 4052 4325 1348 Size: 2704 273 4698 Fragments arranged by size: 4698 2704 273 PciI A'CATG_T Cuts at: 2396 6574 2396 Size: 4178 3497 PflMI CCAn_nnn'nTGG Cuts at: 1108 3814 3907 1108 Size: 2706 93 4876 Fragments arranged by size: 4876 2706 93 PfoI T'CCnGG_A Cuts at: 3540 4146 3540 Size: 606 7069 PmeI GTTT'AAAC Cuts at: 4979 4979 Size: 7675 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5744 5776 7257 7289 5744 Size: 32 1481 32 6130 Fragments arranged by size: 6130 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2893 2935 4153 2893 Size: 42 1218 6415 Fragments arranged by size: 6415 1218 42 PshAI GACnn'nnGTC Cuts at: 1613 2399 1613 Size: 786 6889 PsiI TTA'TAA Cuts at: 5245 5245 Size: 7675 PspOMI G'GGCC_C Cuts at: 2413 3338 4026 4365 4719 2413 Size: 925 688 339 354 5369 Fragments arranged by size: 5369 925 688 354 339 PstI C_TGCA'G Cuts at: 830 2745 3627 3681 3732 3795 3828 3897 3921 Size: 1915 882 54 51 63 33 69 24 Cuts at: 3921 4374 4395 4626 5027 830 Size: 453 21 231 401 3478 Fragments arranged by size: 3478 1915 882 453 401 231 69 63 54 51 33 24 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7675 PvuII CAG'CTG Cuts at: 3091 3613 3853 3916 4196 4240 4685 4864 3091 Size: 522 240 63 280 44 445 179 5902 Fragments arranged by size: 5902 522 445 280 240 179 63 44 SacI G_AGCT'C Cuts at: 3507 3603 4413 3507 Size: 96 810 6769 Fragments arranged by size: 6769 810 96 SalI G'TCGA_C Cuts at: 1936 5017 1936 Size: 3081 4594 SapI GCTCTTCn'nnn_ Cuts at: 3764 3764 Size: 7675 SbfI CC_TGCA'GG Cuts at: 4374 5027 4374 Size: 653 7022 ScaI AGT'ACT Cuts at: 5923 7635 5923 Size: 1712 5963 SfcI C'TryA_G Cuts at: 826 1045 2091 2741 3623 3677 3728 3791 3824 Size: 219 1046 650 882 54 51 63 33 Cuts at: 3824 3893 3917 4197 4370 4391 4622 5023 6158 Size: 69 24 280 173 21 231 401 1135 Cuts at: 6158 6839 7030 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1046 882 681 650 401 280 231 219 191 173 69 63 54 51 33 24 21 SfiI GGCCn_nnn'nGGCC Cuts at: 3956 3956 Size: 7675 SfoI GGC'GCC Cuts at: 4268 4268 Size: 7675 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7675 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7675 SmaI CCC'GGG Cuts at: 1872 4889 5002 1872 Size: 3017 113 4545 Fragments arranged by size: 4545 3017 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 3445 3602 5779 6680 6942 820 Size: 197 926 1502 157 2177 901 262 1553 Fragments arranged by size: 2177 1553 1502 926 901 262 197 157 SnaBI TAC'GTA Cuts at: 493 5466 493 Size: 4973 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7675 SphI G_CATG'C Cuts at: 1132 2696 2962 5033 1132 Size: 1564 266 2071 3774 Fragments arranged by size: 3774 2071 1564 266 SspI AAT'ATT Cuts at: 5 52 5599 5 Size: 47 5547 2081 Fragments arranged by size: 5547 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7675 StyI C'CwwG_G Cuts at: 513 1065 2181 3072 4173 4380 4804 5114 513 Size: 552 1116 891 1101 207 424 310 3074 Fragments arranged by size: 3074 1116 1101 891 552 424 310 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2894 4019 5702 5719 5872 6057 Size: 1255 614 700 1125 1683 17 153 185 Cuts at: 6057 6502 325 Size: 445 1498 Fragments arranged by size: 1683 1498 1255 1125 700 614 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4503 4566 5921 Size: 274 80 33 51 1443 2526 63 1355 Cuts at: 5921 7633 96 Size: 1712 138 Fragments arranged by size: 2526 1712 1443 1355 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4321 4353 7635 7667 4321 Size: 32 3282 32 4329 Fragments arranged by size: 4329 3282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2297 1316 Size: 981 6694 XbaI T'CTAG_A Cuts at: 5011 5011 Size: 7675 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2369 2369 Size: 7675 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7675 XmaI C'CCGG_G Cuts at: 1870 4887 5000 1870 Size: 3017 113 4545 Fragments arranged by size: 4545 3017 113 XmnI GAAnn'nnTTC Cuts at: 5804 5804 Size: 7675 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5483 276 Size: 53 83 186 936 3949 2468 Fragments arranged by size: 3949 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI