(Linear) (Six-base) MAPSORT of: KIBB8549.seq Check: 7604 from: 1 to: 2970 KIBB8549.orf With 182 enzymes: SgfI * July 3, 2012 16:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1203 1385 1826 2282 2970 Size: 1203 182 441 456 688 Fragments arranged by size: 1203 688 456 441 182 Acc65I G'GTAC_C Cuts at: 0 449 2970 Size: 449 2521 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 737 913 1765 1783 2257 2449 2554 Size: 451 286 176 852 18 474 192 105 Cuts at: 2554 2693 2970 Size: 139 277 Fragments arranged by size: 852 474 451 286 277 192 176 139 105 18 AflIII A'CryG_T Cuts at: 0 391 2970 Size: 391 2579 AlwNI CAG_nnn'CTG Cuts at: 0 1266 1809 1848 1884 2625 2970 Size: 1266 543 39 36 741 345 Fragments arranged by size: 1266 741 543 345 39 36 ApaI G_GGCC'C Cuts at: 0 412 1337 2025 2364 2718 2970 Size: 412 925 688 339 354 252 Fragments arranged by size: 925 688 412 354 339 252 ApaLI G'TGCA_C Cuts at: 0 1216 2970 Size: 1216 1754 AvaI C'yCGr_G Cuts at: 0 2882 2970 Size: 2882 88 BamHI G'GATC_C Cuts at: 0 635 2970 Size: 635 2335 BanI G'GyrC_C Cuts at: 0 244 449 2261 2543 2970 Size: 244 205 1812 282 427 Fragments arranged by size: 1812 427 282 244 205 BanII G_rGCy'C Cuts at: 0 412 597 777 1325 1337 1502 1598 2025 Size: 412 185 180 548 12 165 96 427 Cuts at: 2025 2364 2408 2705 2718 2970 Size: 339 44 297 13 252 Fragments arranged by size: 548 427 412 339 297 252 185 180 165 96 44 13 12 BbeI G_GCGC'C Cuts at: 0 2265 2970 Size: 2265 705 BbvCI CC'TCA_GC Cuts at: 0 2123 2970 Size: 2123 847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1658 1692 2845 2879 2970 Size: 1658 34 1153 34 91 Fragments arranged by size: 1658 1153 91 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 162 2970 Size: 162 2808 BglI GCCn_nnn'nGGC Cuts at: 0 1951 2173 2780 2970 Size: 1951 222 607 190 Fragments arranged by size: 1951 607 222 190 BglII A'GATC_T Cuts at: 0 720 2970 Size: 720 2250 BlpI GC'TnA_GC Cuts at: 0 1370 1613 1735 2970 Size: 1370 243 122 1235 Fragments arranged by size: 1370 1235 243 122 Bme1580I G_kGCm'C Cuts at: 0 412 1083 1220 1337 2025 2364 2718 2970 Size: 412 671 137 117 688 339 354 252 Fragments arranged by size: 688 671 412 354 339 252 137 117 BmgBI CAC'GTC Cuts at: 0 1689 2970 Size: 1689 1281 BmrI ACTGGGnnnn_n' Cuts at: 0 2019 2970 Size: 2019 951 BmtI G_CTAG'C Cuts at: 0 463 2970 Size: 463 2507 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 865 2970 Size: 865 2105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 462 877 1288 1411 1498 1504 1558 Size: 460 2 415 411 123 87 6 54 Cuts at: 1558 2173 2495 2632 2970 Size: 615 322 137 338 Fragments arranged by size: 615 460 415 411 338 322 137 123 87 54 6 2 Bpu10I CC'TnA_GC Cuts at: 0 1928 2123 2970 Size: 1928 195 847 Fragments arranged by size: 1928 847 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1461 1582 2970 Size: 1461 121 1388 Fragments arranged by size: 1461 1388 121 BsaI GGTCTCn'nnnn_ Cuts at: 0 1534 1793 2344 2970 Size: 1534 259 551 626 Fragments arranged by size: 1534 626 551 259 BsaAI yAC'GTr Cuts at: 0 2227 2970 Size: 2227 743 BsaHI Gr'CG_yC Cuts at: 0 845 2262 2970 Size: 845 1417 708 Fragments arranged by size: 1417 845 708 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 433 463 2970 Size: 433 30 2507 Fragments arranged by size: 2507 433 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1087 1246 1480 2089 2092 2095 2104 2107 Size: 1087 159 234 609 3 3 9 3 Cuts at: 2107 2110 2113 2116 2119 2221 2224 2572 2605 Size: 3 3 3 3 102 3 348 33 Cuts at: 2605 2970 Size: 365 Fragments arranged by size: 1087 609 365 348 234 159 102 33 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1326 1701 1713 1895 2000 2313 2718 2970 Size: 1326 375 12 182 105 313 405 252 Fragments arranged by size: 1326 405 375 313 252 182 105 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2825 2970 Size: 2825 145 BsiEI CG_ry'CG Cuts at: 0 1496 1557 2649 2970 Size: 1496 61 1092 321 Fragments arranged by size: 1496 1092 321 61 BsiHKAI G_wGCw'C Cuts at: 0 1220 1376 1502 1598 2408 2970 Size: 1220 156 126 96 810 562 Fragments arranged by size: 1220 810 562 156 126 96 BsmI GAATG_Cn' Cuts at: 0 580 2915 2970 Size: 580 2335 55 Fragments arranged by size: 2335 580 55 BsmBI CGTCTCn'nnnn_ Cuts at: 0 836 1264 2970 Size: 836 428 1706 Fragments arranged by size: 1706 836 428 Bsp1286I G_dGCh'C Cuts at: 0 412 597 777 1083 1220 1325 1337 1376 Size: 412 185 180 306 137 105 12 39 Cuts at: 1376 1502 1598 2025 2364 2408 2705 2718 2970 Size: 126 96 427 339 44 297 13 252 Fragments arranged by size: 427 412 339 306 297 252 185 180 137 126 105 96 44 39 13 12 BspHI T'CATG_A Cuts at: 0 279 2970 Size: 279 2691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1095 1203 1385 1826 2282 2970 Size: 1095 108 182 441 456 688 Fragments arranged by size: 1095 688 456 441 182 108 BsrBI CCG'CTC Cuts at: 0 771 1077 1488 1625 2541 2970 Size: 771 306 411 137 916 429 Fragments arranged by size: 916 771 429 411 306 137 BsrDI GCAATG_nn' Cuts at: 0 888 2501 2970 Size: 888 1613 469 Fragments arranged by size: 1613 888 469 BsrFI r'CCGG_y Cuts at: 0 446 1025 2258 2779 2970 Size: 446 579 1233 521 191 Fragments arranged by size: 1233 579 521 446 191 BssHII G'CGCG_C Cuts at: 0 1522 2970 Size: 1522 1448 BssSI C'ACGA_G Cuts at: 0 1900 2970 Size: 1900 1070 BstAPI GCAn_nnn'nTGC Cuts at: 0 421 1194 2250 2853 2912 2970 Size: 421 773 1056 603 59 58 Fragments arranged by size: 1056 773 603 421 59 58 BstXI CCAn_nnnn'nTGG Cuts at: 0 2473 2734 2970 Size: 2473 261 236 Fragments arranged by size: 2473 261 236 BstYI r'GATC_y Cuts at: 0 435 635 720 2970 Size: 435 200 85 2250 Fragments arranged by size: 2250 435 200 85 Bsu36I CC'TnA_GG Cuts at: 0 164 598 850 2300 2970 Size: 164 434 252 1450 670 Fragments arranged by size: 1450 670 434 252 164 BtgI C'CryG_G Cuts at: 0 1067 2168 2375 2970 Size: 1067 1101 207 595 Fragments arranged by size: 1101 1067 595 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2440 2970 Size: 2440 530 BtsI GCAGTG_nn' Cuts at: 0 1669 2534 2790 2837 2970 Size: 1669 865 256 47 133 Fragments arranged by size: 1669 865 256 133 47 EaeI y'GGCC_r Cuts at: 0 29 725 1493 1832 1943 2970 Size: 29 696 768 339 111 1027 Fragments arranged by size: 1027 768 696 339 111 29 EagI C'GGCC_G Cuts at: 0 1493 2970 Size: 1493 1477 EarI CTCTTCn'nnn_ Cuts at: 0 911 1759 2970 Size: 911 848 1211 Fragments arranged by size: 1211 911 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1531 2970 Size: 1531 1439 EcoICRI GAG'CTC Cuts at: 0 1500 1596 2406 2970 Size: 1500 96 810 564 Fragments arranged by size: 1500 810 564 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 460 462 737 877 913 1288 1411 Size: 451 9 2 275 140 36 375 123 Cuts at: 1411 1498 1504 1558 1765 1783 2173 2257 2449 Size: 87 6 54 207 18 390 84 192 Cuts at: 2449 2495 2554 2632 2693 2970 Size: 46 59 78 61 277 Fragments arranged by size: 451 390 375 277 275 207 192 140 123 87 84 78 61 59 54 46 36 18 9 6 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1102 1381 2051 2798 2970 Size: 1102 279 670 747 172 Fragments arranged by size: 1102 747 670 279 172 EcoO109I rG'GnC_Cy Cuts at: 0 408 409 888 930 1333 1334 2148 2361 Size: 408 1 479 42 403 1 814 213 Cuts at: 2361 2714 2715 2970 Size: 353 1 255 Fragments arranged by size: 814 479 408 403 353 255 213 42 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 940 2970 Size: 940 2030 HaeII r_GCGC'y Cuts at: 0 1178 1986 2265 2892 2970 Size: 1178 808 279 627 78 Fragments arranged by size: 1178 808 627 279 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 42 74 2970 Size: 42 32 2896 Fragments arranged by size: 2896 42 32 HincII GTy'rAC Cuts at: 0 830 2970 Size: 830 2140 HindIII A'AGCT_T Cuts at: 0 922 2970 Size: 922 2048 HpaI GTT'AAC Cuts at: 0 830 2970 Size: 830 2140 KasI G'GCGC_C Cuts at: 0 2261 2970 Size: 2261 709 KpnI G_GTAC'C Cuts at: 0 453 2970 Size: 453 2517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 536 746 1658 2970 Size: 536 210 912 1312 Fragments arranged by size: 1312 912 536 210 MscI TGG'CCA Cuts at: 0 727 2970 Size: 727 2243 MslI CAynn'nnrTG Cuts at: 0 259 2970 Size: 259 2711 MspA1I CmG'CkG Cuts at: 0 1086 1608 1848 1911 2191 2235 2680 2859 Size: 1086 522 240 63 280 44 445 179 Cuts at: 2859 2970 Size: 111 Fragments arranged by size: 1086 522 445 280 240 179 111 63 44 NaeI GCC'GGC Cuts at: 0 2260 2781 2970 Size: 2260 521 189 Fragments arranged by size: 2260 521 189 NarI GG'CG_CC Cuts at: 0 2262 2970 Size: 2262 708 NcoI C'CATG_G Cuts at: 0 1067 2168 2375 2970 Size: 1067 1101 207 595 Fragments arranged by size: 1101 1067 595 207 NgoMIV G'CCGG_C Cuts at: 0 2258 2779 2970 Size: 2258 521 191 Fragments arranged by size: 2258 521 191 NheI G'CTAG_C Cuts at: 0 459 2970 Size: 459 2511 NspI r_CATG'y Cuts at: 0 395 691 957 2970 Size: 395 296 266 2013 Fragments arranged by size: 2013 395 296 266 PasI CC'CwG_GG Cuts at: 0 2047 2320 2970 Size: 2047 273 650 Fragments arranged by size: 2047 650 273 PciI A'CATG_T Cuts at: 0 391 2970 Size: 391 2579 PflMI CCAn_nnn'nTGG Cuts at: 0 1809 1902 2970 Size: 1809 93 1068 Fragments arranged by size: 1809 1068 93 PfoI T'CCnGG_A Cuts at: 0 1535 2141 2970 Size: 1535 606 829 Fragments arranged by size: 1535 829 606 PpuMI rG'GwC_Cy Cuts at: 0 888 930 2148 2970 Size: 888 42 1218 822 Fragments arranged by size: 1218 888 822 42 PshAI GACnn'nnGTC Cuts at: 0 394 2970 Size: 394 2576 PspOMI G'GGCC_C Cuts at: 0 408 1333 2021 2360 2714 2970 Size: 408 925 688 339 354 256 Fragments arranged by size: 925 688 408 354 339 256 PstI C_TGCA'G Cuts at: 0 740 1622 1676 1727 1790 1823 1892 1916 Size: 740 882 54 51 63 33 69 24 Cuts at: 1916 2369 2390 2621 2970 Size: 453 21 231 349 Fragments arranged by size: 882 740 453 349 231 69 63 54 51 33 24 21 PvuII CAG'CTG Cuts at: 0 1086 1608 1848 1911 2191 2235 2680 2859 Size: 1086 522 240 63 280 44 445 179 Cuts at: 2859 2970 Size: 111 Fragments arranged by size: 1086 522 445 280 240 179 111 63 44 SacI G_AGCT'C Cuts at: 0 1502 1598 2408 2970 Size: 1502 96 810 562 Fragments arranged by size: 1502 810 562 96 SapI GCTCTTCn'nnn_ Cuts at: 0 1759 2970 Size: 1759 1211 SbfI CC_TGCA'GG Cuts at: 0 2369 2970 Size: 2369 601 SfcI C'TryA_G Cuts at: 0 86 736 1618 1672 1723 1786 1819 1888 Size: 86 650 882 54 51 63 33 69 Cuts at: 1888 1912 2192 2365 2386 2617 2970 Size: 24 280 173 21 231 353 Fragments arranged by size: 882 650 353 280 231 173 86 69 63 54 51 33 24 21 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1951 2970 Size: 1951 1019 SfoI GGC'GCC Cuts at: 0 2263 2970 Size: 2263 707 SmaI CCC'GGG Cuts at: 0 2884 2970 Size: 2884 86 SmlI C'TyrA_G Cuts at: 0 1440 1597 2970 Size: 1440 157 1373 Fragments arranged by size: 1440 1373 157 SphI G_CATG'C Cuts at: 0 691 957 2970 Size: 691 266 2013 Fragments arranged by size: 2013 691 266 StyI C'CwwG_G Cuts at: 0 176 1067 2168 2375 2799 2970 Size: 176 891 1101 207 424 171 Fragments arranged by size: 1101 891 424 207 176 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 189 889 2014 2970 Size: 189 700 1125 956 Fragments arranged by size: 1125 956 700 189 TatI w'GTAC_w Cuts at: 0 2498 2561 2970 Size: 2498 63 409 Fragments arranged by size: 2498 409 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2316 2348 2970 Size: 2316 32 622 Fragments arranged by size: 2316 622 32 Tth111I GACn'n_nGTC Cuts at: 0 292 2970 Size: 292 2678 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 364 2970 Size: 364 2606 XmaI C'CCGG_G Cuts at: 0 2882 2970 Size: 2882 88 Enzymes that do cut: AarI Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SapI SbfI SfcI SfiI SfoI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI BsaBI BsaWI BsiWI BspEI BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI MfeI MluI NdeI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI