(Circular) (Six-base) MAPSORT of: pFN21AE3552.seq Check: 6381 from: 1 to: 10579 pFN21AE3552 10579 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 3452 8389 278 Size: 53 83 186 936 1786 130 4937 2468 Fragments arranged by size: 4937 2468 1786 936 186 130 83 53 AccI GT'mk_AC Cuts at: 1937 6075 6811 7922 1937 Size: 4138 736 1111 4594 Fragments arranged by size: 4594 4138 1111 736 Acc65I G'GTAC_C Cuts at: 7900 7900 Size: 10579 AclI AA'CG_TT Cuts at: 1563 8706 9079 1563 Size: 7143 373 3063 Fragments arranged by size: 7143 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2139 2919 4095 4530 4733 4774 5091 5668 Size: 507 780 1176 435 203 41 317 577 Cuts at: 5668 5867 6057 6525 6936 7032 7063 8642 10026 Size: 199 190 468 411 96 31 1579 1384 Cuts at: 10026 10294 1632 Size: 268 1917 Fragments arranged by size: 1917 1579 1384 1176 780 577 507 468 435 411 317 268 203 199 190 96 41 31 AflII C'TTAA_G Cuts at: 820 1017 3041 5825 820 Size: 197 2024 2784 5574 Fragments arranged by size: 5574 2784 2024 197 AflIII A'CryG_T Cuts at: 1203 2015 7341 9478 10455 1203 Size: 812 5326 2137 977 1327 Fragments arranged by size: 5326 2137 1327 977 812 AgeI A'CCGG_T Cuts at: 7512 9410 7512 Size: 1898 8681 AhdI GACnn_n'nnGTC Cuts at: 4759 4832 9308 4759 Size: 73 4476 6030 Fragments arranged by size: 6030 4476 73 AleI CACnn'nnGTG Cuts at: 1166 2792 1166 Size: 1626 8953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3323 6242 7446 1184 Size: 2139 2919 1204 4317 Fragments arranged by size: 4317 2919 2139 1204 AlwNI CAG_nnn'CTG Cuts at: 2225 6635 8322 9894 2225 Size: 4410 1687 1572 2910 Fragments arranged by size: 4410 2910 1687 1572 ApaLI G'TGCA_C Cuts at: 8636 9792 8636 Size: 1156 9423 ApoI r'AATT_y Cuts at: 1493 3033 3139 3648 3759 3796 5863 7006 7453 Size: 1540 106 509 111 37 2067 1143 447 Cuts at: 7453 7888 8114 8266 10375 1493 Size: 435 226 152 2109 1697 Fragments arranged by size: 2109 2067 1697 1540 1143 509 447 435 226 152 111 106 37 AseI AT'TA_AT Cuts at: 160 1032 4556 5492 9133 160 Size: 872 3524 936 3641 1606 Fragments arranged by size: 3641 3524 1606 936 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10579 AvaI C'yCGr_G Cuts at: 1870 1943 3828 4378 7904 1870 Size: 73 1885 550 3526 4545 Fragments arranged by size: 4545 3526 1885 550 73 AvrII C'CTAG_G Cuts at: 3635 3635 Size: 10579 BamHI G'GATC_C Cuts at: 7909 8276 7909 Size: 367 10212 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7900 9355 618 Size: 325 217 627 6113 1455 1842 Fragments arranged by size: 6113 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6587 6821 7330 7898 1391 Size: 5196 234 509 568 4072 Fragments arranged by size: 5196 4072 568 509 234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 4758 4780 5079 5855 5943 6654 Size: 971 591 2268 22 299 776 88 711 Cuts at: 6654 928 Size: 4853 Fragments arranged by size: 4853 2268 971 776 711 591 299 88 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8770 8804 1903 Size: 34 6833 34 3678 Fragments arranged by size: 6833 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2296 8471 9681 2296 Size: 6175 1210 3194 Fragments arranged by size: 6175 3194 1210 BclI T'GATC_A Cuts at: 1548 4450 1548 Size: 2902 7677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4258 6677 Size: 107 122 71 631 605 337 2249 2419 Cuts at: 6677 9190 136 Size: 2513 1525 Fragments arranged by size: 2513 2419 2249 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3089 3769 10574 3089 Size: 680 6805 3094 Fragments arranged by size: 6805 3094 680 BlpI GC'TnA_GC Cuts at: 4614 6653 7531 7996 4614 Size: 2039 878 465 7197 Fragments arranged by size: 7197 2039 878 465 Bme1580I G_kGCm'C Cuts at: 1790 3348 4021 7425 8640 9796 1790 Size: 1558 673 3404 1215 1156 2573 Fragments arranged by size: 3404 2573 1558 1215 1156 673 BmgBI CAC'GTC Cuts at: 1318 4337 5043 6660 1318 Size: 3019 706 1617 5237 Fragments arranged by size: 5237 3019 1617 706 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3343 4050 6407 Size: 416 53 83 399 24 1926 707 2357 Cuts at: 6407 7242 8310 9268 442 Size: 835 1068 958 1753 Fragments arranged by size: 2357 1926 1753 1068 958 835 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5093 1056 Size: 4037 6542 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 3127 6649 2947 Size: 180 3522 6877 Fragments arranged by size: 6877 3522 180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2995 4083 5163 6790 9239 1239 Size: 1756 1088 1080 1627 2449 2579 Fragments arranged by size: 2579 2449 1756 1627 1088 1080 Bpu10I CC'TnA_GC Cuts at: 5697 7598 5697 Size: 1901 8678 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 2160 2523 3064 3078 3420 5566 8704 9569 Size: 121 363 541 14 342 2146 3138 865 Cuts at: 9569 9867 2039 Size: 298 2751 Fragments arranged by size: 3138 2751 2146 865 541 363 342 298 121 14 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 4289 5003 7312 9242 882 Size: 617 575 2215 714 2309 1930 2219 Fragments arranged by size: 2309 2219 2215 1930 714 617 575 BsaAI yAC'GTr Cuts at: 493 1204 4702 8370 493 Size: 711 3498 3668 2702 Fragments arranged by size: 3668 3498 2702 711 BsaBI GATnn'nnATC Cuts at: 4642 4994 10573 4642 Size: 352 5579 4648 Fragments arranged by size: 5579 4648 352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3449 4237 5442 Size: 53 83 186 936 1786 130 788 1205 Cuts at: 5442 8386 8768 9403 275 Size: 2944 382 635 1451 Fragments arranged by size: 2944 1786 1451 1205 936 788 635 382 186 130 83 53 BsaWI w'CCGG_w Cuts at: 7512 9012 9410 9684 9831 10242 7512 Size: 1500 398 274 147 411 7849 Fragments arranged by size: 7849 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2382 2412 3670 3700 4286 Size: 30 165 30 979 30 1258 30 586 Cuts at: 4286 4316 4513 4534 4543 4564 1178 Size: 30 197 21 9 21 7193 Fragments arranged by size: 7193 1258 979 586 197 165 30 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2283 2658 3744 6386 7620 1186 Size: 1097 375 1086 2642 1234 4145 Fragments arranged by size: 4145 2642 1234 1097 1086 375 BseYI C'CCAG_C Cuts at: 6081 6452 9782 10278 6081 Size: 371 3330 496 6382 Fragments arranged by size: 6382 3330 496 371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6077 1163 Size: 573 4341 5665 Fragments arranged by size: 5665 4341 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7662 8029 8790 9818 1540 Size: 275 186 5661 367 761 1028 2301 Fragments arranged by size: 5661 2301 1028 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 3445 6497 8640 8725 9796 3445 Size: 3052 2143 85 1071 4228 Fragments arranged by size: 4228 3052 2143 1071 85 BsmI GAATG_Cn' Cuts at: 5060 5382 7375 7822 8090 8183 5060 Size: 322 1993 447 268 93 7456 Fragments arranged by size: 7456 1993 447 322 268 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3456 3456 Size: 10579 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3348 3445 4021 6497 6587 6821 7330 Size: 399 1558 97 576 2476 90 234 509 Cuts at: 7330 7425 7898 8640 8725 9796 1391 Size: 95 473 742 85 1071 2174 Fragments arranged by size: 2476 2174 1558 1071 742 576 509 473 399 234 97 95 90 85 BspEI T'CCGG_A Cuts at: 10242 10242 Size: 10579 BspHI T'CATG_A Cuts at: 3334 4453 5394 8468 10198 3334 Size: 1119 941 3074 1730 3715 Fragments arranged by size: 3715 3074 1730 1119 941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2347 4322 7934 844 Size: 1503 1975 3612 3489 Fragments arranged by size: 3612 3489 1975 1503 BsrBI CCG'CTC Cuts at: 1394 1823 7479 8466 1394 Size: 429 5656 987 3507 Fragments arranged by size: 5656 3507 987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2584 2812 3779 6420 7681 9074 9248 Size: 1183 1336 228 967 2641 1261 1393 174 Cuts at: 9248 65 Size: 1396 Fragments arranged by size: 2641 1396 1393 1336 1261 1183 967 228 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7512 9223 9410 1700 Size: 110 5702 1711 187 2869 Fragments arranged by size: 5702 2869 1711 187 110 BsrGI T'GTAC_A Cuts at: 96 2360 4920 96 Size: 2264 2560 5755 Fragments arranged by size: 5755 2560 2264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10579 BssSI C'ACGA_G Cuts at: 7644 8639 9651 7644 Size: 995 1012 8572 Fragments arranged by size: 8572 1012 995 BstAPI GCAn_nnn'nTGC Cuts at: 794 5257 7817 794 Size: 4463 2560 3556 Fragments arranged by size: 4463 3556 2560 BstBI TT'CG_AA Cuts at: 5926 9383 5926 Size: 3457 7122 BstEII G'GTnAC_C Cuts at: 1184 2366 3849 5956 1184 Size: 1182 1483 2107 5807 Fragments arranged by size: 5807 2107 1483 1182 BstXI CCAn_nnnn'nTGG Cuts at: 3019 7711 3019 Size: 4692 5887 BstYI r'GATC_y Cuts at: 1972 3089 3737 3769 5158 5755 6197 7618 7909 Size: 1117 648 32 1389 597 442 1421 291 Cuts at: 7909 8276 8661 8678 10130 10216 10228 10574 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1421 1389 1117 648 597 442 385 367 346 291 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 4214 6822 4214 Size: 2608 7971 BtgI C'CryG_G Cuts at: 513 1065 2643 4874 6834 513 Size: 552 1578 2231 1960 4258 Fragments arranged by size: 4258 2231 1960 1578 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9840 Fragments arranged by size: 9840 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3379 3821 8095 8889 8909 2799 Size: 580 442 4274 794 20 4469 Fragments arranged by size: 4469 4274 794 580 442 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10579 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3571 3606 529 Size: 35 3007 35 7502 Fragments arranged by size: 7502 3007 35 35 DraI TTT'AAA Cuts at: 2937 3754 7883 2937 Size: 817 4129 5633 Fragments arranged by size: 5633 4129 817 DraIII CAC_nnn'GTG Cuts at: 7366 7366 Size: 10579 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2029 4948 5144 9586 809 Size: 507 713 2919 196 4442 1802 Fragments arranged by size: 4442 2919 1802 713 507 196 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2138 4871 Size: 54 1425 50 275 18 180 128 2733 Cuts at: 4871 6735 7563 8026 8915 10544 8 Size: 1864 828 463 889 1629 43 Fragments arranged by size: 2733 1864 1629 1425 889 828 463 275 180 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8026 1537 Size: 275 6214 4090 Fragments arranged by size: 6214 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4953 5127 5304 5711 6033 6618 7082 7683 Size: 3600 174 177 407 322 585 464 601 Cuts at: 7683 8509 1353 Size: 826 3423 Fragments arranged by size: 3600 3423 826 601 585 464 407 322 177 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4380 9166 9540 9686 387 Size: 1064 138 2791 4786 374 146 1280 Fragments arranged by size: 4786 2791 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2919 2995 4083 4095 4530 4733 Size: 393 507 780 76 1088 12 435 203 Cuts at: 4733 4774 5091 5163 5668 5867 6057 6525 6790 Size: 41 317 72 505 199 190 468 265 Cuts at: 6790 6936 7032 7063 8642 9239 10026 10294 1239 Size: 146 96 31 1579 597 787 268 1524 Fragments arranged by size: 1579 1524 1088 787 780 597 507 505 468 435 393 317 268 265 203 199 190 146 96 76 72 41 31 12 EcoNI CCTnn'n_nnAGG Cuts at: 5063 5063 Size: 10579 EcoO109I rG'GnC_Cy Cuts at: 2144 2336 2730 2882 4507 7540 7728 2144 Size: 192 394 152 1625 3033 188 4995 Fragments arranged by size: 4995 3033 1625 394 192 188 152 EcoRI G'AATT_C Cuts at: 7888 8266 7888 Size: 378 10201 EcoRV GAT'ATC Cuts at: 6477 6477 Size: 10579 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4832 6062 4832 Size: 1230 9349 FspI TGC'GCA Cuts at: 9085 9085 Size: 10579 HaeII r_GCGC'y Cuts at: 1678 9726 1678 Size: 8048 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2843 2875 3062 3094 4286 Size: 32 135 32 1271 32 187 32 1192 Cuts at: 4286 4318 4372 4404 4571 4603 4745 4763 4777 Size: 32 54 32 167 32 142 18 14 Cuts at: 4777 4795 4818 4850 6815 6847 7475 7507 7596 Size: 18 23 32 1965 32 628 32 89 Cuts at: 7596 7628 9220 9252 9294 9326 1373 Size: 32 1592 32 42 32 2626 Fragments arranged by size: 2626 1965 1592 1271 1192 628 187 167 142 135 89 54 42 32 32 32 32 32 32 32 32 32 32 32 32 32 23 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 5500 6812 7194 7230 7923 8169 Size: 988 281 3562 1312 382 36 693 246 Cuts at: 8169 669 Size: 3079 Fragments arranged by size: 3562 3079 1312 988 693 382 281 246 36 HindIII A'AGCT_T Cuts at: 748 2565 7777 748 Size: 1817 5212 3550 Fragments arranged by size: 5212 3550 1817 HpaI GTT'AAC Cuts at: 7194 8169 7194 Size: 975 9604 KpnI G_GTAC'C Cuts at: 7904 7904 Size: 10579 MfeI C'AATT_G Cuts at: 8178 8178 Size: 10579 MluI A'CGCG_T Cuts at: 2015 10455 2015 Size: 8440 2139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2000 3200 4849 5070 5295 5470 9693 9877 Size: 695 1200 1649 221 225 175 4223 184 Cuts at: 9877 1305 Size: 2007 Fragments arranged by size: 4223 2007 1649 1200 695 225 221 184 175 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 2140 6737 7565 10 Size: 54 1425 343 180 128 4597 828 3024 Fragments arranged by size: 4597 3024 1425 828 343 180 128 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3069 5814 5963 7428 8537 8896 Size: 648 1626 277 2745 149 1465 1109 359 Cuts at: 8896 9055 518 Size: 159 2042 Fragments arranged by size: 2745 2042 1626 1465 1109 648 359 277 159 149 MspA1I CmG'CkG Cuts at: 1606 6251 6558 7505 7995 8672 9820 10065 1606 Size: 4645 307 947 490 677 1148 245 2120 Fragments arranged by size: 4645 2120 1148 947 677 490 307 245 NcoI C'CATG_G Cuts at: 513 1065 2643 4874 513 Size: 552 1578 2231 6218 Fragments arranged by size: 6218 2231 1578 552 NdeI CA'TA_TG Cuts at: 387 4648 5504 387 Size: 4261 856 5462 Fragments arranged by size: 5462 4261 856 NheI G'CTAG_C Cuts at: 1052 5089 1052 Size: 4037 6542 NotI GC'GGCC_GC Cuts at: 8026 8026 Size: 10579 NspI r_CATG'y Cuts at: 1132 1229 3902 5968 7345 7937 9482 1132 Size: 97 2673 2066 1377 592 1545 2229 Fragments arranged by size: 2673 2229 2066 1545 1377 592 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10579 PciI A'CATG_T Cuts at: 7341 9478 7341 Size: 2137 8442 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10579 PfoI T'CCnGG_A Cuts at: 5109 5560 7115 5109 Size: 451 1555 8573 Fragments arranged by size: 8573 1555 451 PmeI GTTT'AAAC Cuts at: 7883 7883 Size: 10579 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8648 8680 10161 10193 8648 Size: 32 1481 32 9034 Fragments arranged by size: 9034 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2144 2336 2882 4507 7540 2144 Size: 192 546 1625 3033 5183 Fragments arranged by size: 5183 3033 1625 546 192 PshAI GACnn'nnGTC Cuts at: 1613 6131 6807 1613 Size: 4518 676 5385 Fragments arranged by size: 5385 4518 676 PsiI TTA'TAA Cuts at: 2378 3279 8149 2378 Size: 901 4870 4808 Fragments arranged by size: 4870 4808 901 PspXI vC'TCGA_Gb Cuts at: 4378 4378 Size: 10579 PstI C_TGCA'G Cuts at: 830 3007 7813 7931 830 Size: 2177 4806 118 3478 Fragments arranged by size: 4806 3478 2177 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10579 PvuII CAG'CTG Cuts at: 6251 6558 7505 6251 Size: 307 947 9325 Fragments arranged by size: 9325 947 307 SalI G'TCGA_C Cuts at: 1936 6810 7921 1936 Size: 4874 1111 4594 Fragments arranged by size: 4874 4594 1111 SapI GCTCTTCn'nnn_ Cuts at: 4953 6033 7082 4953 Size: 1080 1049 8450 Fragments arranged by size: 8450 1080 1049 SbfI CC_TGCA'GG Cuts at: 3007 7931 3007 Size: 4924 5655 ScaI AGT'ACT Cuts at: 2712 8827 10539 2712 Size: 6115 1712 2752 Fragments arranged by size: 6115 2752 1712 SexAI A'CCwGG_T Cuts at: 2722 3282 2722 Size: 560 10019 SfcI C'TryA_G Cuts at: 826 1045 2501 2734 3003 5427 7809 7927 9062 Size: 219 1456 233 269 2424 2382 118 1135 Cuts at: 9062 9743 9934 826 Size: 681 191 1471 Fragments arranged by size: 2424 2382 1471 1456 1135 681 269 233 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10579 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10579 SmaI CCC'GGG Cuts at: 1872 7906 1872 Size: 6034 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 2175 2538 3041 3079 3093 Size: 197 926 111 121 363 503 38 14 Cuts at: 3093 3435 4378 5581 5825 8683 9584 9846 820 Size: 342 943 1203 244 2858 901 262 1553 Fragments arranged by size: 2858 1553 1203 943 926 901 503 363 342 262 244 197 121 111 38 14 SnaBI TAC'GTA Cuts at: 493 8370 493 Size: 7877 2702 SpeI A'CTAG_T Cuts at: 152 6164 152 Size: 6012 4567 SphI G_CATG'C Cuts at: 1132 7937 1132 Size: 6805 3774 SspI AAT'ATT Cuts at: 5 52 8503 5 Size: 47 8451 2081 Fragments arranged by size: 8451 2081 47 StuI AGG'CCT Cuts at: 1517 2060 6671 1517 Size: 543 4611 5425 Fragments arranged by size: 5425 4611 543 StyI C'CwwG_G Cuts at: 513 1065 2643 3635 4803 4874 7710 8018 513 Size: 552 1578 992 1168 71 2836 308 3074 Fragments arranged by size: 3074 2836 1578 1168 992 552 308 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2385 5975 7264 7676 8606 8623 8776 Size: 1255 805 3590 1289 412 930 17 153 Cuts at: 8776 8961 9406 325 Size: 185 445 1498 Fragments arranged by size: 3590 1498 1289 1255 930 805 445 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2360 2702 2710 Size: 274 80 33 51 1443 383 342 8 Cuts at: 2710 4920 5976 8825 10537 96 Size: 2210 1056 2849 1712 138 Fragments arranged by size: 2849 2210 1712 1443 1056 383 342 274 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4000 4032 5814 5846 10539 10571 4000 Size: 32 1782 32 4693 32 4008 Fragments arranged by size: 4693 4008 1782 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3318 4300 4948 1316 Size: 2002 982 648 6947 Fragments arranged by size: 6947 2002 982 648 XbaI T'CTAG_A Cuts at: 5761 7915 5761 Size: 2154 8425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3679 4589 6595 3679 Size: 910 2006 7663 Fragments arranged by size: 7663 2006 910 XhoI C'TCGA_G Cuts at: 1943 4378 1943 Size: 2435 8144 XmaI C'CCGG_G Cuts at: 1870 7904 1870 Size: 6034 4545 XmnI GAAnn'nnTTC Cuts at: 2487 3484 3874 4771 8708 2487 Size: 997 390 897 3937 4358 Fragments arranged by size: 4358 3937 997 897 390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 3450 8387 276 Size: 53 83 186 936 1786 130 4937 2468 Fragments arranged by size: 4937 2468 1786 936 186 130 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI