################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbvCI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 944 ACACGT A^C[GA][CT]GT Asp700I 609 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 696 TGGCCA TGG^CCA BamHI 735 GGATCC G^GATCC BanI 332 GGTGCC G^G[CT][GA]CC BbrPI 945 CACGTG CAC^GTG BcgI 1155 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 820 GGATAC GTATCC(6/5) BfaI 23 CTAG C^TAG BglII 1013 AGATCT A^GATCT BsaWI 377 ACCGGA [AT]^CCGG[AT] BsmI 1407 GCATTC GAATGC(1/-1) BspDI 281 ATCGAT AT^CGAT BspHI 1316 TCATGA T^CATGA BstXI 593 CCAGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 281 ATCGAT AT^CGAT DdeI 1331 CTGAG C^T[ACGT]AG DraII 779 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 513 CCATGG C^C[GA][CT]GG EarI 490 GAAGAG CTCTTC(1/4) EcoO109I 779 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 513 CCATGG C^C[AT][AT]GG Ksp632I 490 GAAGAG CTCTTC(1/4) MaeI 23 CTAG C^TAG MfeI 1234 CAATTG C^AATTG MluNI 696 TGGCCA TGG^CCA MscI 696 TGGCCA TGG^CCA MunI 1234 CAATTG C^AATTG NcoI 513 CCATGG C^CATGG NspI 401 GCATGC [GA]CATG^[CT] PflMI 497 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 945 CACGTG CAC^GTG PmlI 945 CACGTG CAC^GTG PpuMI 779 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1316 TCATGA T^CATGA SfaNI 284 GATGC GCATC(5/9) SfcI 200 CTACAG C^T[GA][CT]AG SmlI 954 CTTGAG C^T[CT][GA]AG SphI 401 GCATGC GCATG^C StyI 513 CCATGG C^C[AT][AT]GG Van91I 497 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 528 CCACAGTTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 609 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 925 GAGCTC G[AT]GC[AT]^C 957 GAGCTC AvaII 192 GGACC G^G[AT]CC 780 GGACC BbsI 588 GTCTTC GAAGAC(2/6) 987 GTCTTC BpuAI 588 GTCTTC GAAGAC(2/6) 987 GTCTTC BsaAI 945 CACGTG [CT]AC^GT[GA] 963 TACGTG BseRI 1387 CTCCTC GAGGAG(10/8) 1156 GAGGAG BsiHKAI 925 GAGCTC G[AT]GC[AT]^C 957 GAGCTC BsmAI 179 GTCTC GTCTC(1/5) 312 GTCTC BsmFI 8 GGGAC GGGAC(10/14) 191 GGGAC BsrBI 4 GAGCGG CCGCTC(-3/-3) 1105 GAGCGG BstYI 735 GGATCC [GA]^GATC[CT] 1013 AGATCT EaeI 696 TGGCCA [CT]^GGCC[GA] 866 TGGCCG EcoNI 22 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1389 CCTCTTTAAGG EcoRV 441 GATATC GAT^ATC 1198 GATATC HapII 378 CCGG C^CGG 414 CCGG HpaII 378 CCGG C^CGG 414 CCGG MaeII 946 ACGT A^CGT 964 ACGT MflI 735 GGATCC [GA]^GATC[CT] 1013 AGATCT MseI 1181 TTAA T^TAA 1394 TTAA MslI 639 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 798 CACGCCCATG MspI 378 CCGG C^CGG 414 CCGG SacI 925 GAGCTC GAGCT^C 957 GAGCTC ScaI 338 AGTACT AGT^ACT 1046 AGTACT Tru9I 1181 TTAA T^TAA 1394 TTAA XhoII 735 GGATCC [GA]^GATC[CT] 1013 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I