(Circular) (Six-base) MAPSORT of: pFN21AE3212.seq Check: 9576 from: 1 to: 6142 pFN21AE3212 6142 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2614 4205 5589 5857 1632 Size: 982 1591 1384 268 1917 Fragments arranged by size: 1917 1591 1384 982 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 2949 5041 6018 1203 Size: 1746 2092 977 1327 Fragments arranged by size: 2092 1746 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 3885 5457 3885 Size: 1572 4570 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3467 1870 Size: 73 1524 4545 Fragments arranged by size: 4545 1524 73 BamHI G'GATC_C Cuts at: 2740 3472 3839 2740 Size: 732 367 5043 Fragments arranged by size: 5043 732 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2337 3463 4918 618 Size: 325 217 627 550 1126 1455 1842 Fragments arranged by size: 1842 1455 1126 627 550 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2934 2966 3461 1391 Size: 663 880 32 495 4072 Fragments arranged by size: 4072 880 663 495 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2586 2985 928 Size: 971 687 399 4085 Fragments arranged by size: 4085 971 687 399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3149 3183 4333 4367 1903 Size: 34 1212 34 1150 34 3678 Fragments arranged by size: 3678 1212 1150 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2819 4034 5244 2819 Size: 1215 1210 3717 Fragments arranged by size: 3717 1215 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3018 6137 3018 Size: 3119 3023 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2804 3375 4203 5359 1790 Size: 1014 571 828 1156 2573 Fragments arranged by size: 2573 1156 1014 828 571 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 3873 4831 Size: 416 53 83 399 24 1323 1133 958 Cuts at: 4831 442 Size: 1753 Fragments arranged by size: 1753 1323 1133 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2351 2433 2530 3398 4802 1239 Size: 848 264 82 97 868 1404 2579 Fragments arranged by size: 2579 1404 868 848 264 97 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2980 4267 5132 5430 2980 Size: 1287 865 298 3692 Fragments arranged by size: 3692 1287 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2952 2970 3933 493 Size: 711 1748 18 963 2702 Fragments arranged by size: 2702 1748 963 711 18 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 4575 4973 5247 5394 5805 2382 Size: 2193 398 274 147 411 2719 Fragments arranged by size: 2719 2193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3044 3074 1178 Size: 30 165 30 1641 30 4246 Fragments arranged by size: 4246 1641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3176 3383 1186 Size: 1990 207 3945 Fragments arranged by size: 3945 1990 207 BseYI C'CCAG_C Cuts at: 5345 5841 5345 Size: 496 5646 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2934 2966 4203 4288 5359 2934 Size: 32 1237 85 1071 3717 Fragments arranged by size: 3717 1237 1071 85 32 BsmI GAATG_Cn' Cuts at: 3412 3653 3746 3412 Size: 241 93 5808 Fragments arranged by size: 5808 241 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2804 2934 2966 3375 3461 4203 Size: 399 264 750 130 32 409 86 742 Cuts at: 4203 4288 5359 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 750 742 409 399 264 130 86 85 32 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 3321 4031 5761 3321 Size: 710 1730 3702 Fragments arranged by size: 3702 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2011 3112 4029 1394 Size: 429 188 1101 917 3507 Fragments arranged by size: 3507 1101 917 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2605 2605 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2740 3018 3472 3839 4224 4241 5693 5779 Size: 768 278 454 367 385 17 1452 86 Cuts at: 5779 5791 6137 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 768 454 385 367 346 278 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2518 513 Size: 552 939 514 4137 Fragments arranged by size: 4137 939 552 514 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3042 3658 4452 4472 3042 Size: 616 794 20 4712 Fragments arranged by size: 4712 794 616 20 ClaI AT'CG_AT Cuts at: 1554 2287 1554 Size: 733 5409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2701 2871 3589 Size: 54 1425 50 275 18 871 170 718 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 871 718 275 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 4072 1353 Size: 1137 1582 3423 Fragments arranged by size: 3423 1582 1137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4729 5103 5249 387 Size: 1064 138 490 2650 374 146 1280 Fragments arranged by size: 2650 1280 1064 490 374 146 138 EcoICRI GAG'CTC Cuts at: 2932 2964 2932 Size: 32 6110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2351 2433 2530 2614 3398 4205 Size: 393 455 264 82 97 84 784 807 Cuts at: 4205 4802 5589 5857 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 784 597 455 393 268 264 97 84 82 EcoNI CCTnn'n_nnAGG Cuts at: 2031 3398 2031 Size: 1367 4775 EcoO109I rG'GnC_Cy Cuts at: 2785 2785 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 EcoRV GAT'ATC Cuts at: 2448 3205 2448 Size: 757 5385 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3037 3037 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 4783 4815 4857 Size: 32 135 32 1160 32 2019 32 42 Cuts at: 4857 4889 1373 Size: 32 2626 Fragments arranged by size: 2626 2019 1160 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3239 3741 3239 Size: 502 5640 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5256 5440 1305 Size: 3951 184 2007 Fragments arranged by size: 3951 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2703 10 Size: 54 1425 343 871 3449 Fragments arranged by size: 3449 1425 871 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 2807 4100 4459 4618 518 Size: 648 1482 159 1293 359 159 2042 Fragments arranged by size: 2042 1482 1293 648 359 159 159 MspA1I CmG'CkG Cuts at: 1606 2770 3232 3385 3558 4235 5383 5628 1606 Size: 1164 462 153 173 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 677 462 245 173 153 NcoI C'CATG_G Cuts at: 513 1065 2004 2518 513 Size: 552 939 514 4137 Fragments arranged by size: 4137 939 552 514 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2410 3500 5045 1132 Size: 97 1181 1090 1545 2229 Fragments arranged by size: 2229 1545 1181 1090 97 PasI CC'CwG_GG Cuts at: 1348 2543 1348 Size: 1195 4947 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2508 1108 Size: 1400 4742 PfoI T'CCnGG_A Cuts at: 2064 2064 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2952 2952 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 2785 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2770 3232 3385 2770 Size: 462 153 5527 Fragments arranged by size: 5527 462 153 SacI G_AGCT'C Cuts at: 2934 2966 2934 Size: 32 6110 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 2345 3053 4390 6102 2345 Size: 708 1337 1712 2385 Fragments arranged by size: 2385 1712 1337 708 SfcI C'TryA_G Cuts at: 826 1045 2205 3490 4625 5306 5497 826 Size: 219 1160 1285 1135 681 191 1471 Fragments arranged by size: 1471 1285 1160 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2959 4246 5147 5409 820 Size: 197 926 1016 1287 901 262 1553 Fragments arranged by size: 1553 1287 1016 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 2410 3500 1132 Size: 1278 1090 3774 Fragments arranged by size: 3774 1278 1090 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2004 2518 3581 513 Size: 552 939 514 1063 3074 Fragments arranged by size: 3074 1063 939 552 514 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 2640 3051 Size: 274 80 33 51 1443 366 297 411 Cuts at: 3051 4388 6100 96 Size: 1337 1712 138 Fragments arranged by size: 1712 1443 1337 411 366 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2640 2672 2817 2849 2936 2968 3348 3380 6102 Size: 32 145 32 87 32 380 32 2722 Cuts at: 6102 6134 2640 Size: 32 2648 Fragments arranged by size: 2722 2648 380 145 87 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2540 2540 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 2618 4271 2618 Size: 1653 4489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI