################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SphI Sse8387I SspI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Alw44I 2000 GTGCAC G^TGCAC ApaLI 2000 GTGCAC G^TGCAC AspI 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1835 TGGCCA TGG^CCA BamHI 1791 GGATCC G^GATCC BbsI 112 GTCTTC GAAGAC(2/6) BglII 854 AGATCT A^GATCT BpuAI 112 GTCTTC GAAGAC(2/6) BsaI 703 GGTCTC GGTCTC(1/5) BsmAI 704 GTCTC GTCTC(1/5) Bsp1407I 889 TGTACA T^GTACA BsrGI 889 TGTACA T^GTACA BstXI 1169 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EarI 1786 GAAGAG CTCTTC(1/4) EcoNI 2037 CCTAGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 829 GAATTC G^AATTC HgaI 110 GCGTC GACGC(5/10) HincII 656 GTCAAC GT[CT]^[GA]AC HindII 656 GTCAAC GT[CT]^[GA]AC Ksp632I 1786 GAAGAG CTCTTC(1/4) KspI 129 CCGCGG CCGC^GG MaeII 949 ACGT A^CGT MluNI 1835 TGGCCA TGG^CCA MscI 1835 TGGCCA TGG^CCA MslI 1729 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflFI 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2065 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1413 AGGTCCT [GA]G^G[AT]CC[CT] SacII 129 CCGCGG CCGC^GG SgrAI 88 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1318 CTCAAG C^T[CT][GA]AG SspBI 889 TGTACA T^GTACA StuI 1353 AGGCCT AGG^CCT Tth111I 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2065 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 995 CCATTCCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcyI 86 GGCGCC G[GA]^CG[CT]C 92 GGCGCC AfaI 453 GTAC GT^AC 890 GTAC AhdI 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2048 GACAAACTGTC AlwI 1792 GATCC GGATC(4/5) 1791 GGATC ApaI 281 GGGCCC GGGCC^C 665 GGGCCC AspEI 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2048 GACAAACTGTC BbeI 86 GGCGCC GGCGC^C 92 GGCGCC BbvCI 294 CCTCAGC CCTCAGC(-5/-2) 873 CCTCAGC BglI 71 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 878 GCCCCTCTGGC BsaHI 86 GGCGCC G[GA]^CG[CT]C 92 GGCGCC BsgI 387 GTGCAG GTGCAG(16/14) 812 GTGCAG BssSI 312 CTCGTG CACGAG(-5/-1) 1589 CTCGTG BstYI 854 AGATCT [GA]^GATC[CT] 1791 GGATCC DpnII 855 GATC ^GATC 1792 GATC Eam1105I 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2048 GACAAACTGTC HaeII 86 GGCGCC [GA]GCGC^[CT] 92 GGCGCC Hin1I 86 GGCGCC G[GA]^CG[CT]C 92 GGCGCC KasI 86 GGCGCC G^GCGCC 92 GGCGCC MboI 855 GATC ^GATC 1792 GATC MflI 854 AGATCT [GA]^GATC[CT] 1791 GGATCC NaeI 83 GCCGGC GCC^GGC 89 GCCGGC NarI 86 GGCGCC GG^CGCC 92 GGCGCC NcoI 910 CCATGG C^CATGG 1004 CCATGG NdeI 1181 CATATG CA^TATG 2020 CATATG NdeII 855 GATC ^GATC 1792 GATC NgoMIV 83 GCCGGC G^CCGGC 89 GCCGGC NotI 49 GCGGCCGC GC^GGCCGC 131 GCGGCCGC NspI 45 ACATGC [GA]CATG^[CT] 640 ACATGC RsaI 453 GTAC GT^AC 890 GTAC Sau3AI 855 GATC ^GATC 1792 GATC SfoI 86 GGCGCC GGC^GCC 92 GGCGCC SmaI 143 CCCGGG CCC^GGG 225 CCCGGG SpeI 1360 ACTAGT A^CTAGT 1375 ACTAGT TaqI 194 TCGA T^CGA 1981 TCGA TthHB8I 194 TCGA T^CGA 1981 TCGA XhoII 854 AGATCT [GA]^GATC[CT] 1791 GGATCC XmaCI 143 CCCGGG C^CCGGG 225 CCCGGG XmaI 143 CCCGGG C^CCGGG 225 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcsI AluI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I Tsp509I TspRI