(Circular) (Six-base) MAPSORT of: pFN21AE4212.seq Check: 6618 from: 1 to: 6793 pFN21AE4212 6793 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4603 278 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4136 1937 Size: 2199 4594 Acc65I G'GTAC_C Cuts at: 4114 4114 Size: 6793 AclI AA'CG_TT Cuts at: 1563 4920 5293 1563 Size: 3357 373 3063 Fragments arranged by size: 3357 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 4856 6240 6508 1632 Size: 1056 2168 1384 268 1917 Fragments arranged by size: 2168 1917 1384 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 5692 6669 1203 Size: 4489 977 1327 Fragments arranged by size: 4489 1327 977 AgeI A'CCGG_T Cuts at: 5624 5624 Size: 6793 AhdI GACnn_n'nnGTC Cuts at: 2026 4058 5522 2026 Size: 2032 1464 3297 Fragments arranged by size: 3297 2032 1464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6793 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6793 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2180 Size: 32 6761 AlwNI CAG_nnn'CTG Cuts at: 4536 6108 4536 Size: 1572 5221 ApaI G_GGCC'C Cuts at: 2290 2674 2290 Size: 384 6409 ApaLI G'TGCA_C Cuts at: 4005 4850 6006 4005 Size: 845 1156 4792 Fragments arranged by size: 4792 1156 845 ApoI r'AATT_y Cuts at: 1493 2834 2934 3091 3272 3540 4102 4328 4480 Size: 1341 100 157 181 268 562 226 152 Cuts at: 4480 6589 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1341 562 268 226 181 157 152 100 AseI AT'TA_AT Cuts at: 160 1032 5347 160 Size: 872 4315 1606 Fragments arranged by size: 4315 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2148 2230 2387 4118 1870 Size: 73 205 82 157 1731 4545 Fragments arranged by size: 4545 1731 205 157 82 73 BamHI G'GATC_C Cuts at: 3796 4123 4490 3796 Size: 327 367 6099 Fragments arranged by size: 6099 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2097 2371 2511 4114 Size: 325 217 627 304 6 274 140 1603 Cuts at: 4114 5569 618 Size: 1455 1842 Fragments arranged by size: 1842 1603 1455 627 325 304 274 217 140 6 BanII G_rGCy'C Cuts at: 1391 2012 2290 2674 3322 4000 4016 4112 1391 Size: 621 278 384 648 678 16 96 4072 Fragments arranged by size: 4072 678 648 621 384 278 96 16 BbeI G_GCGC'C Cuts at: 2095 2101 2095 Size: 6 6787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 928 Size: 971 211 5611 Fragments arranged by size: 5611 971 211 BbvCI CC'TCA_GC Cuts at: 2300 2879 2300 Size: 579 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4984 5018 1903 Size: 34 3047 34 3678 Fragments arranged by size: 3678 3047 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4685 5895 4685 Size: 1210 5583 BclI T'GATC_A Cuts at: 1548 1548 Size: 6793 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2082 2889 5404 Size: 107 122 71 631 605 410 807 2515 Cuts at: 5404 136 Size: 1525 Fragments arranged by size: 2515 1525 807 631 605 410 122 107 71 BglII A'GATC_T Cuts at: 2859 6788 2859 Size: 3929 2864 BlpI GC'TnA_GC Cuts at: 4210 4210 Size: 6793 Bme1580I G_kGCm'C Cuts at: 1790 2290 2376 2516 2674 2954 3207 3464 4009 Size: 500 86 140 158 280 253 257 545 Cuts at: 4009 4024 4854 6010 1790 Size: 15 830 1156 2573 Fragments arranged by size: 2573 1156 830 545 500 280 257 253 158 140 86 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6793 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3131 4524 5482 Size: 416 53 83 399 24 1714 1393 958 Cuts at: 5482 442 Size: 1753 Fragments arranged by size: 1753 1714 1393 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2038 3970 2038 Size: 1932 4861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2952 3126 3334 3469 5453 1239 Size: 1133 580 174 208 135 1984 2579 Fragments arranged by size: 2579 1984 1133 580 208 174 135 Bpu10I CC'TnA_GC Cuts at: 2280 2300 2879 2280 Size: 20 579 6194 Fragments arranged by size: 6194 579 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3308 4918 5783 6081 3308 Size: 1610 865 298 4020 Fragments arranged by size: 4020 1610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2714 5456 882 Size: 617 1215 2742 2219 Fragments arranged by size: 2742 2219 1215 617 BsaAI yAC'GTr Cuts at: 493 1204 4584 493 Size: 711 3380 2702 Fragments arranged by size: 3380 2702 711 BsaBI GATnn'nnATC Cuts at: 6787 6787 Size: 6793 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 2098 4600 4982 Size: 53 83 186 936 559 6 2502 382 Cuts at: 4982 5617 275 Size: 635 1451 Fragments arranged by size: 2502 1451 936 635 559 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 5226 5624 5898 6045 6456 5226 Size: 398 274 147 411 5563 Fragments arranged by size: 5563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2234 2264 1178 Size: 30 165 30 777 30 24 30 5707 Fragments arranged by size: 5707 777 165 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2255 2288 2477 3123 1186 Size: 1036 33 33 189 646 4856 Fragments arranged by size: 4856 1036 646 189 33 33 BseYI C'CCAG_C Cuts at: 2947 3332 3870 5996 6492 2947 Size: 385 538 2126 496 3248 Fragments arranged by size: 3248 2126 538 496 385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2413 2838 1163 Size: 573 677 425 5118 Fragments arranged by size: 5118 677 573 425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2089 2140 4243 5004 6032 Size: 275 186 57 31 51 2103 761 1028 Cuts at: 6032 1540 Size: 2301 Fragments arranged by size: 2301 2103 1028 761 275 186 57 51 31 BsiHKAI G_wGCw'C Cuts at: 2968 3380 4009 4854 4939 6010 2968 Size: 412 629 845 85 1071 3751 Fragments arranged by size: 3751 1071 845 629 412 85 BsmI GAATG_Cn' Cuts at: 3021 3477 3650 4304 4397 3021 Size: 456 173 654 93 5417 Fragments arranged by size: 5417 654 456 173 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2290 2376 2516 2674 2954 2968 Size: 399 222 278 86 140 158 280 14 Cuts at: 2968 3207 3322 3380 3464 4000 4009 4016 4024 Size: 239 115 58 84 536 9 7 8 Cuts at: 4024 4112 4854 4939 6010 1391 Size: 88 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 280 278 239 222 158 140 115 88 86 85 84 58 14 9 8 7 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 4682 6412 4682 Size: 1730 5063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 3045 3498 4148 844 Size: 1508 693 453 650 3489 Fragments arranged by size: 3489 1508 693 650 453 BsrBI CCG'CTC Cuts at: 1394 1823 2284 2316 3963 4680 1394 Size: 429 461 32 1647 717 3507 Fragments arranged by size: 3507 1647 717 461 429 32 BsrDI GCAATG_nn' Cuts at: 65 1248 2985 2994 3126 5288 5462 65 Size: 1183 1737 9 132 2162 174 1396 Fragments arranged by size: 2162 1737 1396 1183 174 132 9 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2094 3605 5437 5624 1700 Size: 110 278 6 1511 1832 187 2869 Fragments arranged by size: 2869 1832 1511 278 187 110 6 BsrGI T'GTAC_A Cuts at: 96 2894 96 Size: 2798 3995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 2317 3594 4853 5865 2317 Size: 1277 1259 1012 3245 Fragments arranged by size: 3245 1277 1259 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6793 BstBI TT'CG_AA Cuts at: 5597 5597 Size: 6793 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6793 BstXI CCAn_nnnn'nTGG Cuts at: 3181 3181 Size: 6793 BstYI r'GATC_y Cuts at: 1972 2859 3796 4123 4490 4875 4892 6344 6430 Size: 887 937 327 367 385 17 1452 86 Cuts at: 6430 6442 6788 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 937 887 385 367 346 327 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2134 2915 3009 513 Size: 552 939 130 781 94 4297 Fragments arranged by size: 4297 939 781 552 130 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2028 487 Size: 683 56 802 5252 Fragments arranged by size: 5252 802 683 56 BtsI GCAGTG_nn' Cuts at: 2614 2773 2826 3793 3895 4309 5103 5123 2614 Size: 159 53 967 102 414 794 20 4284 Fragments arranged by size: 4284 967 794 414 159 102 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3282 3317 529 Size: 35 2718 35 4005 Fragments arranged by size: 4005 2718 35 35 DraI TTT'AAA Cuts at: 4097 4097 Size: 6793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5800 809 Size: 507 4484 1802 Fragments arranged by size: 4484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2086 2137 Size: 54 1425 50 275 18 225 31 51 Cuts at: 2137 3435 3603 3840 4240 5129 6758 8 Size: 1298 168 237 400 889 1629 43 Fragments arranged by size: 1629 1425 1298 889 400 275 237 225 168 54 51 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2055 2086 2137 4240 1537 Size: 275 243 31 51 2103 4090 Fragments arranged by size: 4090 2103 275 243 51 31 EarI CTCTTCn'nnn_ Cuts at: 1353 3786 4723 1353 Size: 2433 937 3423 Fragments arranged by size: 3423 2433 937 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2201 2231 2324 3795 5380 5754 Size: 1064 138 612 30 93 1471 1585 374 Cuts at: 5754 5900 387 Size: 146 1280 Fragments arranged by size: 1585 1471 1280 1064 612 374 146 138 93 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2688 2952 3126 3334 3469 4856 Size: 393 740 316 264 174 208 135 1387 Cuts at: 4856 5453 6240 6508 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1387 787 740 597 393 316 268 264 208 174 135 EcoNI CCTnn'n_nnAGG Cuts at: 4046 4046 Size: 6793 EcoO109I rG'GnC_Cy Cuts at: 2287 2670 3419 2287 Size: 383 749 5661 Fragments arranged by size: 5661 749 383 EcoRI G'AATT_C Cuts at: 2834 4102 4480 2834 Size: 1268 378 5147 Fragments arranged by size: 5147 1268 378 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 2095 2101 5940 1678 Size: 417 6 3839 2531 Fragments arranged by size: 3839 2531 417 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2617 2649 2950 Size: 32 135 32 440 32 573 32 301 Cuts at: 2950 2982 3899 3923 3931 3955 4044 4076 5434 Size: 32 917 24 8 24 89 32 1358 Cuts at: 5434 5466 5508 5540 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1358 917 573 440 301 135 89 42 32 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2663 4137 4383 669 Size: 988 281 725 1474 246 3079 Fragments arranged by size: 3079 1474 988 725 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6793 HpaI GTT'AAC Cuts at: 4383 4383 Size: 6793 KasI G'GCGC_C Cuts at: 2091 2097 2091 Size: 6 6787 KpnI G_GTAC'C Cuts at: 4118 4118 Size: 6793 MfeI C'AATT_G Cuts at: 4392 4392 Size: 6793 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3016 5907 6091 1305 Size: 1711 2891 184 2007 Fragments arranged by size: 2891 2007 1711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3842 10 Size: 54 1425 343 2010 2961 Fragments arranged by size: 2961 2010 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3738 4751 5110 5269 518 Size: 648 2572 1013 359 159 2042 Fragments arranged by size: 2572 2042 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2136 2257 2304 2382 2508 2562 2947 Size: 476 54 121 47 78 126 54 385 Cuts at: 2947 3700 4209 4886 6034 6279 1606 Size: 753 509 677 1148 245 2120 Fragments arranged by size: 2120 1148 753 677 509 476 385 245 126 121 78 54 54 47 NaeI GCC'GGC Cuts at: 2090 2096 2090 Size: 6 6787 NarI GG'CG_CC Cuts at: 2092 2098 2092 Size: 6 6787 NcoI C'CATG_G Cuts at: 513 1065 2004 2915 3009 513 Size: 552 939 911 94 4297 Fragments arranged by size: 4297 939 911 552 94 NdeI CA'TA_TG Cuts at: 387 3187 4026 387 Size: 2800 839 3154 Fragments arranged by size: 3154 2800 839 NgoMIV G'CCGG_C Cuts at: 2088 2094 2088 Size: 6 6787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 2055 2137 4240 2055 Size: 82 2103 4608 Fragments arranged by size: 4608 2103 82 NspI r_CATG'y Cuts at: 1132 1229 2054 2649 4151 5696 1132 Size: 97 825 595 1502 1545 2229 Fragments arranged by size: 2229 1545 1502 825 595 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6793 PciI A'CATG_T Cuts at: 5692 5692 Size: 6793 PflMI CCAn_nnn'nTGG Cuts at: 1108 4076 1108 Size: 2968 3825 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2212 4862 4894 6375 6407 2180 Size: 32 2650 32 1481 32 2566 Fragments arranged by size: 2650 2566 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3419 3419 Size: 6793 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6793 PsiI TTA'TAA Cuts at: 4363 4363 Size: 6793 PspOMI G'GGCC_C Cuts at: 2286 2670 2286 Size: 384 6409 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2450 2482 2450 Size: 32 6761 PstI C_TGCA'G Cuts at: 830 3151 3809 3976 4145 830 Size: 2321 658 167 169 3478 Fragments arranged by size: 3478 2321 658 169 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6793 PvuII CAG'CTG Cuts at: 2304 2382 2562 2947 3700 2304 Size: 78 180 385 753 5397 Fragments arranged by size: 5397 753 385 180 78 SacII CC_GC'GG Cuts at: 2137 2137 Size: 6793 SalI G'TCGA_C Cuts at: 1936 4135 1936 Size: 2199 4594 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 5041 6753 5041 Size: 1712 5081 SfcI C'TryA_G Cuts at: 826 1045 3147 3393 3805 3972 4141 5276 5957 Size: 219 2102 246 412 167 169 1135 681 Cuts at: 5957 6148 826 Size: 191 1471 Fragments arranged by size: 2102 1471 1135 681 412 246 219 191 169 167 SfoI GGC'GCC Cuts at: 2093 2099 2093 Size: 6 6787 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 2094 1700 Size: 394 6399 SmaI CCC'GGG Cuts at: 1872 2150 2232 4120 1872 Size: 278 82 1888 4545 Fragments arranged by size: 4545 1888 278 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 3323 4897 5798 6060 820 Size: 197 926 1380 1574 901 262 1553 Fragments arranged by size: 1574 1553 1380 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4584 493 Size: 4091 2702 SpeI A'CTAG_T Cuts at: 152 3365 3380 152 Size: 3213 15 3565 Fragments arranged by size: 3565 3213 15 SphI G_CATG'C Cuts at: 1132 4151 1132 Size: 3019 3774 SrfI GCCC'GGGC Cuts at: 2150 2232 2150 Size: 82 6711 SspI AAT'ATT Cuts at: 5 52 4717 5 Size: 47 4665 2081 Fragments arranged by size: 4665 2081 47 StuI AGG'CCT Cuts at: 1517 3360 1517 Size: 1843 4950 StyI C'CwwG_G Cuts at: 513 1065 2004 2915 3009 3851 4000 4232 513 Size: 552 939 911 94 842 149 232 3074 Fragments arranged by size: 3074 939 911 842 552 232 149 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3112 4820 4837 4990 5175 5620 325 Size: 1255 1532 1708 17 153 185 445 1498 Fragments arranged by size: 1708 1532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2894 5039 6751 Size: 274 80 33 51 1443 917 2145 1712 Cuts at: 6751 96 Size: 138 Fragments arranged by size: 2145 1712 1443 917 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 3246 3278 6753 6785 2234 Size: 32 980 32 3475 32 2242 Fragments arranged by size: 3475 2242 980 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3118 1316 Size: 1802 4991 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 3007 Size: 6793 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6793 XmaI C'CCGG_G Cuts at: 1870 2148 2230 4118 1870 Size: 278 82 1888 4545 Fragments arranged by size: 4545 1888 278 82 XmnI GAAnn'nnTTC Cuts at: 4922 4922 Size: 6793 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4601 276 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SanDI SapI SexAI SfiI SwaI