(Linear) (Six-base) MAPSORT of: KIEE4212.seq Check: 5031 from: 1 to: 2088 KIEE4212.orf With 182 enzymes: SgfI * July 3, 2012 18:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 2088 Size: 683 1405 AhdI GACnn_n'nnGTC Cuts at: 0 21 2053 2088 Size: 21 2032 35 Fragments arranged by size: 2032 35 21 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 175 207 2088 Size: 175 32 1881 Fragments arranged by size: 1881 175 32 ApaI G_GGCC'C Cuts at: 0 285 669 2088 Size: 285 384 1419 Fragments arranged by size: 1419 384 285 ApaLI G'TGCA_C Cuts at: 0 2000 2088 Size: 2000 88 ApoI r'AATT_y Cuts at: 0 829 929 1086 1267 1535 2088 Size: 829 100 157 181 268 553 Fragments arranged by size: 829 553 268 181 157 100 AvaI C'yCGr_G Cuts at: 0 143 225 382 2088 Size: 143 82 157 1706 Fragments arranged by size: 1706 157 143 82 BamHI G'GATC_C Cuts at: 0 1791 2088 Size: 1791 297 BanI G'GyrC_C Cuts at: 0 86 92 366 506 2088 Size: 86 6 274 140 1582 Fragments arranged by size: 1582 274 140 86 6 BanII G_rGCy'C Cuts at: 0 7 285 669 1317 1995 2011 2088 Size: 7 278 384 648 678 16 77 Fragments arranged by size: 678 648 384 278 77 16 7 BbeI G_GCGC'C Cuts at: 0 90 96 2088 Size: 90 6 1992 Fragments arranged by size: 1992 90 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 105 2088 Size: 105 1983 BbvCI CC'TCA_GC Cuts at: 0 295 874 2088 Size: 295 579 1214 Fragments arranged by size: 1214 579 295 BglI GCCn_nnn'nGGC Cuts at: 0 77 884 2088 Size: 77 807 1204 Fragments arranged by size: 1204 807 77 BglII A'GATC_T Cuts at: 0 854 2088 Size: 854 1234 Bme1580I G_kGCm'C Cuts at: 0 285 371 511 669 949 1202 1459 2004 Size: 285 86 140 158 280 253 257 545 Cuts at: 2004 2019 2088 Size: 15 69 Fragments arranged by size: 545 285 280 257 253 158 140 86 69 15 BmrI ACTGGGnnnn_n' Cuts at: 0 1126 2088 Size: 1126 962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 33 1965 2088 Size: 33 1932 123 Fragments arranged by size: 1932 123 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 947 1121 1329 1464 2088 Size: 367 580 174 208 135 624 Fragments arranged by size: 624 580 367 208 174 135 Bpu10I CC'TnA_GC Cuts at: 0 275 295 874 2088 Size: 275 20 579 1214 Fragments arranged by size: 1214 579 275 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1303 2088 Size: 1303 785 BsaI GGTCTCn'nnnn_ Cuts at: 0 709 2088 Size: 709 1379 BsaHI Gr'CG_yC Cuts at: 0 87 93 2088 Size: 87 6 1995 Fragments arranged by size: 1995 87 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 229 259 2088 Size: 175 30 24 30 1829 Fragments arranged by size: 1829 175 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 217 250 283 472 1118 2088 Size: 217 33 33 189 646 970 Fragments arranged by size: 970 646 217 189 33 33 BseYI C'CCAG_C Cuts at: 0 942 1327 1865 2088 Size: 942 385 538 223 Fragments arranged by size: 942 538 385 223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 408 833 2088 Size: 408 425 1255 Fragments arranged by size: 1255 425 408 BsiEI CG_ry'CG Cuts at: 0 53 84 135 2088 Size: 53 31 51 1953 Fragments arranged by size: 1953 53 51 31 BsiHKAI G_wGCw'C Cuts at: 0 963 1375 2004 2088 Size: 963 412 629 84 Fragments arranged by size: 963 629 412 84 BsmI GAATG_Cn' Cuts at: 0 1016 1472 1645 2088 Size: 1016 456 173 443 Fragments arranged by size: 1016 456 443 173 Bsp1286I G_dGCh'C Cuts at: 0 7 285 371 511 669 949 963 1202 Size: 7 278 86 140 158 280 14 239 Cuts at: 1202 1317 1375 1459 1995 2004 2011 2019 2088 Size: 115 58 84 536 9 7 8 69 Fragments arranged by size: 536 280 278 239 158 140 115 86 84 69 58 14 9 8 7 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 347 1040 1493 2088 Size: 347 693 453 595 Fragments arranged by size: 693 595 453 347 BsrBI CCG'CTC Cuts at: 0 279 311 1958 2088 Size: 279 32 1647 130 Fragments arranged by size: 1647 279 130 32 BsrDI GCAATG_nn' Cuts at: 0 980 989 1121 2088 Size: 980 9 132 967 Fragments arranged by size: 980 967 132 9 BsrFI r'CCGG_y Cuts at: 0 83 89 1600 2088 Size: 83 6 1511 488 Fragments arranged by size: 1511 488 83 6 BsrGI T'GTAC_A Cuts at: 0 889 2088 Size: 889 1199 BssSI C'ACGA_G Cuts at: 0 312 1589 2088 Size: 312 1277 499 Fragments arranged by size: 1277 499 312 BstXI CCAn_nnnn'nTGG Cuts at: 0 1176 2088 Size: 1176 912 BstYI r'GATC_y Cuts at: 0 854 1791 2088 Size: 854 937 297 Fragments arranged by size: 937 854 297 BtgI C'CryG_G Cuts at: 0 129 910 1004 2088 Size: 129 781 94 1084 Fragments arranged by size: 1084 781 129 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 23 2088 Size: 23 2065 BtsI GCAGTG_nn' Cuts at: 0 609 768 821 1788 1890 2088 Size: 609 159 53 967 102 198 Fragments arranged by size: 967 609 198 159 102 53 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1277 1312 2088 Size: 1277 35 776 Fragments arranged by size: 1277 776 35 EaeI y'GGCC_r Cuts at: 0 50 81 132 1430 1598 1835 2088 Size: 50 31 51 1298 168 237 253 Fragments arranged by size: 1298 253 237 168 51 50 31 EagI C'GGCC_G Cuts at: 0 50 81 132 2088 Size: 50 31 51 1956 Fragments arranged by size: 1956 51 50 31 EarI CTCTTCn'nnn_ Cuts at: 0 1781 2088 Size: 1781 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 196 226 319 1790 2088 Size: 196 30 93 1471 298 Fragments arranged by size: 1471 298 196 93 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 683 947 1121 1329 1464 2088 Size: 367 316 264 174 208 135 624 Fragments arranged by size: 624 367 316 264 208 174 135 EcoNI CCTnn'n_nnAGG Cuts at: 0 2041 2088 Size: 2041 47 EcoO109I rG'GnC_Cy Cuts at: 0 282 665 1414 2088 Size: 282 383 749 674 Fragments arranged by size: 749 674 383 282 EcoRI G'AATT_C Cuts at: 0 829 2088 Size: 829 1259 HaeII r_GCGC'y Cuts at: 0 90 96 2088 Size: 90 6 1992 Fragments arranged by size: 1992 90 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 7 39 612 644 945 977 1894 1918 Size: 7 32 573 32 301 32 917 24 Cuts at: 1918 1926 1950 2039 2071 2088 Size: 8 24 89 32 17 Fragments arranged by size: 917 573 301 89 32 32 32 32 24 24 17 8 7 HincII GTy'rAC Cuts at: 0 658 2088 Size: 658 1430 KasI G'GCGC_C Cuts at: 0 86 92 2088 Size: 86 6 1996 Fragments arranged by size: 1996 86 6 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1011 2088 Size: 1011 1077 MscI TGG'CCA Cuts at: 0 1837 2088 Size: 1837 251 MslI CAynn'nnrTG Cuts at: 0 1733 2088 Size: 1733 355 MspA1I CmG'CkG Cuts at: 0 77 131 252 299 377 503 557 942 Size: 77 54 121 47 78 126 54 385 Cuts at: 942 1695 2088 Size: 753 393 Fragments arranged by size: 753 393 385 126 121 78 77 54 54 47 NaeI GCC'GGC Cuts at: 0 85 91 2088 Size: 85 6 1997 Fragments arranged by size: 1997 85 6 NarI GG'CG_CC Cuts at: 0 87 93 2088 Size: 87 6 1995 Fragments arranged by size: 1995 87 6 NcoI C'CATG_G Cuts at: 0 910 1004 2088 Size: 910 94 1084 Fragments arranged by size: 1084 910 94 NdeI CA'TA_TG Cuts at: 0 1182 2021 2088 Size: 1182 839 67 Fragments arranged by size: 1182 839 67 NgoMIV G'CCGG_C Cuts at: 0 83 89 2088 Size: 83 6 1999 Fragments arranged by size: 1999 83 6 NotI GC'GGCC_GC Cuts at: 0 50 132 2088 Size: 50 82 1956 Fragments arranged by size: 1956 82 50 NspI r_CATG'y Cuts at: 0 49 644 2088 Size: 49 595 1444 Fragments arranged by size: 1444 595 49 PflMI CCAn_nnn'nTGG Cuts at: 0 2071 2088 Size: 2071 17 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 175 207 2088 Size: 175 32 1881 Fragments arranged by size: 1881 175 32 PpuMI rG'GwC_Cy Cuts at: 0 1414 2088 Size: 1414 674 PspOMI G'GGCC_C Cuts at: 0 281 665 2088 Size: 281 384 1423 Fragments arranged by size: 1423 384 281 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 445 477 2088 Size: 445 32 1611 Fragments arranged by size: 1611 445 32 PstI C_TGCA'G Cuts at: 0 1146 1804 1971 2088 Size: 1146 658 167 117 Fragments arranged by size: 1146 658 167 117 PvuII CAG'CTG Cuts at: 0 299 377 557 942 1695 2088 Size: 299 78 180 385 753 393 Fragments arranged by size: 753 393 385 299 180 78 SacII CC_GC'GG Cuts at: 0 132 2088 Size: 132 1956 SfcI C'TryA_G Cuts at: 0 1142 1388 1800 1967 2088 Size: 1142 246 412 167 121 Fragments arranged by size: 1142 412 246 167 121 SfoI GGC'GCC Cuts at: 0 88 94 2088 Size: 88 6 1994 Fragments arranged by size: 1994 88 6 SgrAI Cr'CCGG_yG Cuts at: 0 89 2088 Size: 89 1999 SmaI CCC'GGG Cuts at: 0 145 227 2088 Size: 145 82 1861 Fragments arranged by size: 1861 145 82 SmlI C'TyrA_G Cuts at: 0 1318 2088 Size: 1318 770 SpeI A'CTAG_T Cuts at: 0 1360 1375 2088 Size: 1360 15 713 Fragments arranged by size: 1360 713 15 SrfI GCCC'GGGC Cuts at: 0 145 227 2088 Size: 145 82 1861 Fragments arranged by size: 1861 145 82 StuI AGG'CCT Cuts at: 0 1355 2088 Size: 1355 733 StyI C'CwwG_G Cuts at: 0 910 1004 1846 1995 2088 Size: 910 94 842 149 93 Fragments arranged by size: 910 842 149 94 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1107 2088 Size: 1107 981 TatI w'GTAC_w Cuts at: 0 889 2088 Size: 889 1199 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 229 261 1241 1273 2088 Size: 229 32 980 32 815 Fragments arranged by size: 980 815 229 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1113 2088 Size: 1113 975 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1002 2088 Size: 1002 1086 XmaI C'CCGG_G Cuts at: 0 143 225 2088 Size: 143 82 1863 Fragments arranged by size: 1863 143 82 Enzymes that do cut: AcuI AhdI AloI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PflMI PpiI PpuMI PspOMI PsrI PstI PvuII SacII SfcI SfoI SgrAI SmaI SmlI SpeI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AlwNI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoICRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SphI SspI SwaI XbaI XhoI XmnI ZraI