################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 154 GTATAC GT^[AC][GT]AC AccIII 433 TCCGGA T^CCGGA AcsI 1119 AAATTT [GA]^AATT[CT] AflIII 1092 ACATGT A^C[GA][CT]GT AlwNI 85 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 336 CCTCAGG CC^T[ACGT]AGG Aor51HI 138 AGCGCT AGC^GCT ApoI 1119 AAATTT [GA]^AATT[CT] AspHI 3 GAGCTC G[AT]GC[AT]^C AviII 1341 TGCGCA TGC^GCA BanII 3 GAGCTC G[GA]GC[CT]^C BbsI 317 GAAGAC GAAGAC(2/6) BclI 897 TGATCA T^GATCA BfaI 284 CTAG C^TAG BglII 1317 AGATCT A^GATCT BpuAI 317 GAAGAC GAAGAC(2/6) BsaBI 172 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 433 TCCGGA T^CCGGA BseRI 429 CTCCTC GAGGAG(10/8) BsiHKAI 3 GAGCTC G[AT]GC[AT]^C BsmAI 685 GTCTC GTCTC(1/5) BspEI 433 TCCGGA T^CCGGA BspHI 483 TCATGA T^CATGA BspLU11I 1092 ACATGT A^CATGT BsrI 551 ACTGG ACTGG(1/-1) Bst1107I 154 GTATAC GTA^TAC BstF5I 207 GGATG GGATG(2/0) BstZ17I 154 GTATAC GTA^TAC Bsu36I 336 CCTCAGG CC^T[ACGT]AGG DraII 279 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 957 CGGCCA [CT]^GGCC[GA] EarI 1060 GAAGAG CTCTTC(1/4) Eco47III 138 AGCGCT AGC^GCT Eco81I 336 CCTCAGG CC^T[ACGT]AGG EcoO109I 279 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 870 ATGCAT ATGCA^T FauI 413 CCCGC CCCGC(4/6) FbaI 897 TGATCA T^GATCA FokI 207 GGATG GGATG(9/13) FspI 1341 TGCGCA TGC^GCA HaeII 138 AGCGCT [GA]GCGC^[CT] HincII 1153 GTCAAC GT[CT]^[GA]AC HindII 1153 GTCAAC GT[CT]^[GA]AC HphI 422 TCACC GGTGA(8/7) Ksp632I 1060 GAAGAG CTCTTC(1/4) MaeI 284 CTAG C^TAG MamI 172 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 433 TCCGGA T^CCGGA NdeI 1267 CATATG CA^TATG NlaIV 15 GGAGCC GG[ACGT]^[ACGT]CC NsiI 870 ATGCAT ATGCA^T PacI 1390 TTAATTAA TTAAT^TAA PflMI 232 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1014 GACTC GAGTC(4/5) PvuII 1067 CAGCTG CAG^CTG RcaI 483 TCATGA T^CATGA SacI 3 GAGCTC GAGCT^C SmlI 224 CTCAAG C^T[CT][GA]AG SphI 868 GCATGC GCATG^C SspI 818 AATATT AAT^ATT StuI 1175 AGGCCT AGG^CCT TfiI 779 GAATC G^A[AT]TC Tsp45I 703 GTGAC ^GT[GC]AC TspRI 1132 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 232 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BsaWI 433 TCCGGA [AT]^CCGG[AT] 917 TCCGGT BsmFI 1089 GGGAC GGGAC(10/14) 99 GTCCC DraI 622 TTTAAA TTT^AAA 711 TTTAAA HapII 434 CCGG C^CGG 918 CCGG HinfI 779 GAATC G^A[ACGT]TC 1014 GACTC HpaII 434 CCGG C^CGG 918 CCGG MspA1I 19 CCGCTG C[AC]G^C[GT]G 1067 CAGCTG MspI 434 CCGG C^CGG 918 CCGG NspI 868 GCATGC [GA]CATG^[CT] 1092 ACATGT PstI 937 CTGCAG CTGCA^G 1321 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I XhoII