(Linear) (Six-base) MAPSORT of: KIEE4131.seq Check: 5396 from: 1 to: 1425 KIEE4131.orf With 182 enzymes: SgfI * July 3, 2012 18:37 .. AccI GT'mk_AC Cuts at: 0 155 1425 Size: 155 1270 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 655 1122 1373 1425 Size: 655 467 251 52 Fragments arranged by size: 655 467 251 52 AfeI AGC'GCT Cuts at: 0 140 1425 Size: 140 1285 AflIII A'CryG_T Cuts at: 0 1092 1425 Size: 1092 333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 103 1425 Size: 103 1322 AlwNI CAG_nnn'CTG Cuts at: 0 90 1425 Size: 90 1335 ApoI r'AATT_y Cuts at: 0 1119 1425 Size: 1119 306 BanII G_rGCy'C Cuts at: 0 7 1425 Size: 7 1418 BbsI GAAGACnn'nnnn_ Cuts at: 0 324 1425 Size: 324 1101 BclI T'GATC_A Cuts at: 0 897 1425 Size: 897 528 BfrBI ATG'CAT Cuts at: 0 872 1425 Size: 872 553 BglII A'GATC_T Cuts at: 0 1317 1425 Size: 1317 108 Bme1580I G_kGCm'C Cuts at: 0 1038 1236 1425 Size: 1038 198 189 Fragments arranged by size: 1038 198 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 112 1219 1425 Size: 54 58 1107 206 Fragments arranged by size: 1107 206 58 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 1425 Size: 209 1216 BsaBI GATnn'nnATC Cuts at: 0 176 1425 Size: 176 1249 BsaWI w'CCGG_w Cuts at: 0 433 917 1425 Size: 433 484 508 Fragments arranged by size: 508 484 433 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 420 1425 Size: 420 1005 BseYI C'CCAG_C Cuts at: 0 343 450 998 1425 Size: 343 107 548 427 Fragments arranged by size: 548 427 343 107 BsiHKAI G_wGCw'C Cuts at: 0 7 1425 Size: 7 1418 Bsp1286I G_dGCh'C Cuts at: 0 7 1038 1236 1425 Size: 7 1031 198 189 Fragments arranged by size: 1031 198 189 7 BspEI T'CCGG_A Cuts at: 0 433 1425 Size: 433 992 BspHI T'CATG_A Cuts at: 0 483 1425 Size: 483 942 BstYI r'GATC_y Cuts at: 0 122 510 1317 1425 Size: 122 388 807 108 Fragments arranged by size: 807 388 122 108 BstZ17I GTA'TAC Cuts at: 0 156 1425 Size: 156 1269 Bsu36I CC'TnA_GG Cuts at: 0 337 1425 Size: 337 1088 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 849 1425 Size: 849 576 DraI TTT'AAA Cuts at: 0 624 713 1425 Size: 624 89 712 Fragments arranged by size: 712 624 89 EaeI y'GGCC_r Cuts at: 0 957 1425 Size: 957 468 EarI CTCTTCn'nnn_ Cuts at: 0 1055 1425 Size: 1055 370 EcoICRI GAG'CTC Cuts at: 0 5 1425 Size: 5 1420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 112 655 1122 1219 1373 1425 Size: 54 58 543 467 97 154 52 Fragments arranged by size: 543 467 154 97 58 54 52 EcoO109I rG'GnC_Cy Cuts at: 0 280 1425 Size: 280 1145 FspI TGC'GCA Cuts at: 0 1343 1425 Size: 1343 82 HaeII r_GCGC'y Cuts at: 0 142 1425 Size: 142 1283 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1302 1334 1425 Size: 1302 32 91 Fragments arranged by size: 1302 91 32 HincII GTy'rAC Cuts at: 0 1155 1425 Size: 1155 270 MslI CAynn'nnrTG Cuts at: 0 85 488 1271 1425 Size: 85 403 783 154 Fragments arranged by size: 783 403 154 85 MspA1I CmG'CkG Cuts at: 0 21 1069 1425 Size: 21 1048 356 Fragments arranged by size: 1048 356 21 NdeI CA'TA_TG Cuts at: 0 1268 1425 Size: 1268 157 NsiI A_TGCA'T Cuts at: 0 874 1425 Size: 874 551 NspI r_CATG'y Cuts at: 0 872 1096 1425 Size: 872 224 329 Fragments arranged by size: 872 329 224 PacI TTA_AT'TAA Cuts at: 0 1394 1425 Size: 1394 31 PciI A'CATG_T Cuts at: 0 1092 1425 Size: 1092 333 PflMI CCAn_nnn'nTGG Cuts at: 0 238 1425 Size: 238 1187 PfoI T'CCnGG_A Cuts at: 0 744 1425 Size: 744 681 PsiI TTA'TAA Cuts at: 0 953 1425 Size: 953 472 PstI C_TGCA'G Cuts at: 0 941 1325 1425 Size: 941 384 100 Fragments arranged by size: 941 384 100 PvuII CAG'CTG Cuts at: 0 1069 1425 Size: 1069 356 SacI G_AGCT'C Cuts at: 0 7 1425 Size: 7 1418 SfcI C'TryA_G Cuts at: 0 218 585 937 1321 1425 Size: 218 367 352 384 104 Fragments arranged by size: 384 367 352 218 104 SmlI C'TyrA_G Cuts at: 0 224 1425 Size: 224 1201 SphI G_CATG'C Cuts at: 0 872 1425 Size: 872 553 SspI AAT'ATT Cuts at: 0 820 1425 Size: 820 605 StuI AGG'CCT Cuts at: 0 1177 1425 Size: 1177 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 408 1425 Size: 408 1017 TatI w'GTAC_w Cuts at: 0 542 564 665 1425 Size: 542 22 101 760 Fragments arranged by size: 760 542 101 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 541 573 1425 Size: 541 32 852 Fragments arranged by size: 852 541 32 Enzymes that do cut: AccI AcuI AfeI AflIII AlfI AlwNI ApoI BanII BbsI BclI BfrBI BglII Bme1580I BpmI BpuEI BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BstYI BstZ17I Bsu36I BtgZI DraI EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII MslI MspA1I NdeI NsiI NspI PacI PciI PflMI PfoI PsiI PstI PvuII SacI SfcI SmlI SphI SspI StuI TaqII TatI TstI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI