################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## Acc65I AccI AccIII AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 1553 GACGTC GACGT^C AclI 824 AACGTT AA^CGTT AcyI 1553 GACGTC G[GA]^CG[CT]C AfaI 197 GTAC GT^AC AvaII 958 GGTCC G^G[AT]CC BalI 17 TGGCCA TGG^CCA BcnI 100 CCCGG CC^[GC]GG BsaBI 538 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1553 GACGTC G[GA]^CG[CT]C BsaI 1112 GAGACC GGTCTC(1/5) BsiYI 1640 CCAGCTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1640 CCAGCTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 463 CGTCTC CGTCTC(1/5) BsmFI 1312 GTCCC GGGAC(10/14) BsmI 1342 GAATGC GAATGC(1/-1) BspMI 1215 GCAGGT ACCTGC(4/8) BstBI 395 TTCGAA TT^CGAA BstNI 210 CCAGG CC^[AT]GG Cfr13I 958 GGTCC G^G[ACGT]CC DraIII 436 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 46 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 62 CCGCGG C^C[GA][CT]GG EaeI 17 TGGCCA [CT]^GGCC[GA] EarI 1147 GAAGAG CTCTTC(1/4) EcoRII 210 CCAGG ^CC[AT]GG EcoRV 865 GATATC GAT^ATC EcoT22I 801 ATGCAT ATGCA^T HapII 101 CCGG C^CGG Hin1I 1553 GACGTC G[GA]^CG[CT]C HpaII 101 CCGG C^CGG Ksp632I 1147 GAAGAG CTCTTC(1/4) KspI 62 CCGCGG CCGC^GG MamI 538 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 533 CAATTG C^AATTG MluNI 17 TGGCCA TGG^CCA MscI 17 TGGCCA TGG^CCA MslI 763 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 101 CCGG C^CGG MunI 533 CAATTG C^AATTG MvaI 210 CCAGG CC^[AT]GG NciI 100 CCCGG CC^[GC]GG NsiI 801 ATGCAT ATGCA^T NspI 1607 ACATGC [GA]CATG^[CT] NspV 395 TTCGAA TT^CGAA PflMI 1640 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 824 AACGTT AA^CGTT PvuII 1544 CAGCTG CAG^CTG RsaI 197 GTAC GT^AC SacII 62 CCGCGG CCGC^GG Sau96I 958 GGTCC G^G[ACGT]CC SfcI 119 CTATAG C^T[GA][CT]AG SfuI 395 TTCGAA TT^CGAA SspI 973 AATATT AAT^ATT StuI 1387 AGGCCT AGG^CCT Tsp45I 716 GTGAC ^GT[GC]AC Van91I 1640 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1074 TCTAGA T^CTAGA XcmI 297 CCAGATGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 63 CGCG CG^CG 415 CGCG AciI 62 CCGC CCGC(-3/-1) 64 GCGG AlwI 610 GATCC GGATC(4/5) 991 GGATC BbsI 877 GAAGAC GAAGAC(2/6) 1132 GAAGAC BbvI 1636 GCAGC GCAGC(8/12) 1091 GCTGC BpmI 1490 CTCCAG CTGGAG(16/14) 1109 CTGGAG BpuAI 877 GAAGAC GAAGAC(2/6) 1132 GAAGAC BsaJI 62 CCGCGG C^C[ACGT][ACGT]GG 210 CCAGGG BseRI 1252 GAGGAG GAGGAG(10/8) 1307 GAGGAG BsmAI 1112 GAGAC GTCTC(1/5) 464 GTCTC BssKI 100 CCCGG ^CC[ACGT]GG 210 CCAGG BstUI 63 CGCG CG^CG 415 CGCG BstYI 609 AGATCC [GA]^GATC[CT] 991 GGATCT Fnu4HI 1091 GCTGC GC^[ACGT]GC 1636 GCAGC HaeIII 18 GGCC GG^CC 1388 GGCC HincII 222 GTTGAC GT[CT]^[GA]AC 1524 GTTGAC HindII 222 GTTGAC GT[CT]^[GA]AC 1524 GTTGAC HindIII 367 AAGCTT A^AGCTT 1193 AAGCTT ItaI 1091 GCTGC GC^[ACGT]GC 1636 GCAGC MflI 609 AGATCC [GA]^GATC[CT] 991 GGATCT MspA1I 62 CCGCGG C[AC]G^C[GT]G 1544 CAGCTG MvnI 63 CGCG CG^CG 415 CGCG PleI 283 GAGTC GAGTC(4/5) 1310 GAGTC ScrFI 100 CCCGG CC^[ACGT]GG 210 CCAGG SfaNI 1159 GATGC GCATC(5/9) 616 GCATC TaqI 396 TCGA T^CGA 1557 TCGA TseI 1091 GCTGC G^C[AT]GC 1636 GCAGC TthHB8I 396 TCGA T^CGA 1557 TCGA XhoII 609 AGATCC [GA]^GATC[CT] 991 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AluI ApoI Asp700I BfaI BsrI BstF5I Cac8I DdeI DpnII EcoRI FokI HinfI HphI MaeI MaeII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI TfiI Tru9I Tsp509I TspRI XmnI