(Circular) (Six-base) MAPSORT of: pFN21AE3237.seq Check: 376 from: 1 to: 6361 pFN21AE3237 6361 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 4171 278 Size: 53 83 186 936 2026 609 2468 Fragments arranged by size: 2468 2026 936 609 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 2830 4488 4861 1563 Size: 1267 1658 373 3063 Fragments arranged by size: 3063 1658 1267 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 3447 4424 5808 6076 1632 Size: 406 1409 977 1384 268 1917 Fragments arranged by size: 1917 1409 1384 977 406 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 5260 6237 1203 Size: 4057 977 1327 Fragments arranged by size: 4057 1327 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6361 AlwNI CAG_nnn'CTG Cuts at: 4104 5676 4104 Size: 1572 4789 ApaLI G'TGCA_C Cuts at: 4418 5574 4418 Size: 1156 5205 ApoI r'AATT_y Cuts at: 1493 2404 2486 2519 2696 3002 3188 3248 3386 Size: 911 82 33 177 306 186 60 138 Cuts at: 3386 3670 3896 4048 6157 1493 Size: 284 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 306 284 226 186 177 152 138 82 60 33 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 3686 1870 Size: 73 1743 4545 Fragments arranged by size: 4545 1743 73 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3682 5137 618 Size: 325 217 627 1895 1455 1842 Fragments arranged by size: 1895 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3680 1391 Size: 2289 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 3144 928 Size: 971 990 255 4145 Fragments arranged by size: 4145 990 971 255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4552 4586 1903 Size: 34 2615 34 3678 Fragments arranged by size: 3678 2615 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BfrBI ATG'CAT Cuts at: 2808 2808 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4972 136 Size: 107 122 71 631 605 3300 1525 Fragments arranged by size: 3300 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6356 6356 Size: 6361 BlpI GC'TnA_GC Cuts at: 3778 3778 Size: 6361 Bme1580I G_kGCm'C Cuts at: 1790 4422 5578 1790 Size: 2632 1156 2573 Fragments arranged by size: 2632 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4092 5050 442 Size: 416 53 83 399 24 2675 958 1753 Fragments arranged by size: 2675 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3135 3480 5021 1239 Size: 1896 345 1541 2579 Fragments arranged by size: 2579 1896 1541 345 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4486 5351 5649 4486 Size: 865 298 5198 Fragments arranged by size: 5198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3111 5024 882 Size: 617 1612 1913 2219 Fragments arranged by size: 2219 1913 1612 617 BsaAI yAC'GTr Cuts at: 493 1204 4152 493 Size: 711 2948 2702 Fragments arranged by size: 2948 2702 711 BsaBI GATnn'nnATC Cuts at: 2547 6355 2547 Size: 3808 2553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 4168 4550 5185 Size: 53 83 186 936 2026 609 382 635 Cuts at: 5185 275 Size: 1451 Fragments arranged by size: 2026 1451 936 635 609 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4794 5192 5466 5613 6024 4794 Size: 398 274 147 411 5131 Fragments arranged by size: 5131 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3478 3508 1178 Size: 30 165 30 2075 30 4031 Fragments arranged by size: 4031 2075 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3272 3327 1186 Size: 2086 55 4220 Fragments arranged by size: 4220 2086 55 BseYI C'CCAG_C Cuts at: 5564 6060 5564 Size: 496 5865 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5788 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4422 4507 5578 4422 Size: 85 1071 5205 Fragments arranged by size: 5205 1071 85 BsmI GAATG_Cn' Cuts at: 3353 3872 3965 3353 Size: 519 93 5749 Fragments arranged by size: 5749 519 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2474 2474 Size: 6361 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3680 4422 4507 5578 1391 Size: 399 1890 742 85 1071 2174 Fragments arranged by size: 2174 1890 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3211 3716 844 Size: 2367 505 3489 Fragments arranged by size: 3489 2367 505 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4856 5030 65 Size: 1183 3608 174 1396 Fragments arranged by size: 3608 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6361 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6361 BstBI TT'CG_AA Cuts at: 2401 5165 2401 Size: 2764 3597 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6361 BstYI r'GATC_y Cuts at: 1972 2614 2996 3691 4058 4443 4460 5912 5998 Size: 642 382 695 367 385 17 1452 86 Cuts at: 5998 6010 6356 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 695 642 385 382 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 513 Size: 552 939 63 4807 Fragments arranged by size: 4807 939 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5622 Fragments arranged by size: 5622 683 56 BtsI GCAGTG_nn' Cuts at: 2565 3475 3877 4671 4691 2565 Size: 910 402 794 20 4235 Fragments arranged by size: 4235 910 794 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3665 3665 Size: 6361 DraIII CAC_nnn'GTG Cuts at: 2446 2446 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2057 5368 809 Size: 507 741 3311 1802 Fragments arranged by size: 3311 1802 741 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3808 4697 Size: 54 1425 50 275 18 192 1786 889 Cuts at: 4697 6326 8 Size: 1629 43 Fragments arranged by size: 1786 1629 1425 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 4291 1353 Size: 1794 1144 3423 Fragments arranged by size: 3423 1794 1144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4948 5322 5468 387 Size: 1064 138 3359 374 146 1280 Fragments arranged by size: 3359 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 3135 3447 3480 4424 5021 5808 Size: 393 406 1097 312 33 944 597 787 Cuts at: 5808 6076 1239 Size: 268 1524 Fragments arranged by size: 1524 1097 944 787 597 406 393 312 268 33 EcoRI G'AATT_C Cuts at: 3002 3188 3386 3670 4048 3002 Size: 186 198 284 378 5315 Fragments arranged by size: 5315 378 284 198 186 EcoRV GAT'ATC Cuts at: 2872 2872 Size: 6361 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2906 2906 Size: 6361 FspI TGC'GCA Cuts at: 4867 4867 Size: 6361 HaeII r_GCGC'y Cuts at: 1678 5508 1678 Size: 3830 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2606 2638 3131 3163 5002 Size: 32 135 32 1034 32 493 32 1839 Cuts at: 5002 5034 5076 5108 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1839 1034 493 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2229 3531 3705 3951 669 Size: 988 281 291 1302 174 246 3079 Fragments arranged by size: 3079 1302 988 291 281 246 174 HindIII A'AGCT_T Cuts at: 748 2372 3198 748 Size: 1624 826 3911 Fragments arranged by size: 3911 1624 826 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 2538 3960 2538 Size: 1422 4939 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 2882 5475 5659 1305 Size: 970 607 2593 184 2007 Fragments arranged by size: 2593 2007 970 607 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 10 Size: 54 1425 343 192 4347 Fragments arranged by size: 4347 1425 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2772 4319 4678 4837 518 Size: 648 1606 1547 359 159 2042 Fragments arranged by size: 2042 1606 1547 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 3551 3777 4454 5602 5847 1606 Size: 463 1482 226 677 1148 245 2120 Fragments arranged by size: 2120 1482 1148 677 463 245 226 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4870 Fragments arranged by size: 4870 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NsiI A_TGCA'T Cuts at: 2810 2810 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 3616 3719 5264 1132 Size: 97 2387 103 1545 2229 Fragments arranged by size: 2387 2229 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6361 PciI A'CATG_T Cuts at: 5260 5260 Size: 6361 PflMI CCAn_nnn'nTGG Cuts at: 1108 3651 1108 Size: 2543 3818 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3430 3462 4430 4462 5943 5975 3430 Size: 32 968 32 1481 32 3816 Fragments arranged by size: 3816 1481 968 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6361 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PstI C_TGCA'G Cuts at: 830 3713 830 Size: 2883 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 3551 3551 Size: 6361 SacII CC_GC'GG Cuts at: 2070 2070 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 4609 6321 4609 Size: 1712 4649 SfcI C'TryA_G Cuts at: 826 1045 2124 3709 4844 5525 5716 826 Size: 219 1079 1585 1135 681 191 1471 Fragments arranged by size: 1585 1471 1135 1079 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3688 1872 Size: 1816 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4465 5366 5628 820 Size: 197 926 2522 901 262 1553 Fragments arranged by size: 2522 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SspI AAT'ATT Cuts at: 5 52 2980 4285 5 Size: 47 2928 1305 2081 Fragments arranged by size: 2928 2081 1305 47 StuI AGG'CCT Cuts at: 1517 3394 1517 Size: 1877 4484 StyI C'CwwG_G Cuts at: 513 1065 2004 3800 513 Size: 552 939 1796 3074 Fragments arranged by size: 3074 1796 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 4388 4405 4558 4743 5188 325 Size: 1255 717 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4607 6319 96 Size: 274 80 33 51 1443 2630 1712 138 Fragments arranged by size: 2630 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6321 6353 6321 Size: 32 6329 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3079 3697 3079 Size: 618 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2309 2309 Size: 6361 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6361 XmaI C'CCGG_G Cuts at: 1870 3686 1870 Size: 1816 4545 XmnI GAAnn'nnTTC Cuts at: 2100 2831 2949 4490 2100 Size: 731 118 1541 3971 Fragments arranged by size: 3971 1541 731 118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 4169 276 Size: 53 83 186 936 2026 609 2468 Fragments arranged by size: 2468 2026 936 609 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI