(Linear) (Six-base) MAPSORT of: KIEE3237.seq Check: 2233 from: 1 to: 1656 KIEE3237.orf With 182 enzymes: SgfI * July 3, 2012 17:30 .. AatII G_ACGT'C Cuts at: 0 1557 1656 Size: 1557 99 AclI AA'CG_TT Cuts at: 0 825 1656 Size: 825 831 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 1442 1656 Size: 33 1409 214 Fragments arranged by size: 1409 214 33 ApoI r'AATT_y Cuts at: 0 399 481 514 691 997 1183 1243 1381 Size: 399 82 33 177 306 186 60 138 Cuts at: 1381 1656 Size: 275 Fragments arranged by size: 399 306 275 186 177 138 82 60 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 884 1139 1656 Size: 884 255 517 Fragments arranged by size: 884 517 255 BfrBI ATG'CAT Cuts at: 0 803 1656 Size: 803 853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1130 1475 1656 Size: 1130 345 181 Fragments arranged by size: 1130 345 181 BsaI GGTCTCn'nnnn_ Cuts at: 0 1106 1656 Size: 1106 550 BsaBI GATnn'nnATC Cuts at: 0 542 1656 Size: 542 1114 BsaHI Gr'CG_yC Cuts at: 0 1554 1656 Size: 1554 102 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1473 1503 1656 Size: 1473 30 153 Fragments arranged by size: 1473 153 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1267 1322 1656 Size: 1267 55 334 Fragments arranged by size: 1267 334 55 BsmI GAATG_Cn' Cuts at: 0 1348 1656 Size: 1348 308 BsmBI CGTCTCn'nnnn_ Cuts at: 0 469 1656 Size: 469 1187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1206 1656 Size: 1206 450 BstBI TT'CG_AA Cuts at: 0 396 1656 Size: 396 1260 BstYI r'GATC_y Cuts at: 0 609 991 1656 Size: 609 382 665 Fragments arranged by size: 665 609 382 BtgI C'CryG_G Cuts at: 0 62 1656 Size: 62 1594 BtsI GCAGTG_nn' Cuts at: 0 560 1470 1656 Size: 560 910 186 Fragments arranged by size: 910 560 186 DraIII CAC_nnn'GTG Cuts at: 0 441 1656 Size: 441 1215 DrdI GACnn_nn'nnGTC Cuts at: 0 52 1656 Size: 52 1604 EaeI y'GGCC_r Cuts at: 0 17 1656 Size: 17 1639 EarI CTCTTCn'nnn_ Cuts at: 0 1142 1656 Size: 1142 514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 1130 1442 1475 1656 Size: 33 1097 312 33 181 Fragments arranged by size: 1097 312 181 33 33 EcoRI G'AATT_C Cuts at: 0 997 1183 1381 1656 Size: 997 186 198 275 Fragments arranged by size: 997 275 198 186 EcoRV GAT'ATC Cuts at: 0 867 1656 Size: 867 789 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 901 1656 Size: 901 755 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 601 633 1126 1158 1656 Size: 601 32 493 32 498 Fragments arranged by size: 601 498 493 32 32 HincII GTy'rAC Cuts at: 0 224 1526 1656 Size: 224 1302 130 Fragments arranged by size: 1302 224 130 HindIII A'AGCT_T Cuts at: 0 367 1193 1656 Size: 367 826 463 Fragments arranged by size: 826 463 367 MfeI C'AATT_G Cuts at: 0 533 1656 Size: 533 1123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 270 877 1656 Size: 270 607 779 Fragments arranged by size: 779 607 270 MscI TGG'CCA Cuts at: 0 19 1656 Size: 19 1637 MslI CAynn'nnrTG Cuts at: 0 767 1656 Size: 767 889 MspA1I CmG'CkG Cuts at: 0 64 1546 1656 Size: 64 1482 110 Fragments arranged by size: 1482 110 64 NsiI A_TGCA'T Cuts at: 0 805 1656 Size: 805 851 NspI r_CATG'y Cuts at: 0 1611 1656 Size: 1611 45 PflMI CCAn_nnn'nTGG Cuts at: 0 1646 1656 Size: 1646 10 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1425 1457 1656 Size: 1425 32 199 Fragments arranged by size: 1425 199 32 PvuII CAG'CTG Cuts at: 0 1546 1656 Size: 1546 110 SacII CC_GC'GG Cuts at: 0 65 1656 Size: 65 1591 SfcI C'TryA_G Cuts at: 0 119 1656 Size: 119 1537 SspI AAT'ATT Cuts at: 0 975 1656 Size: 975 681 StuI AGG'CCT Cuts at: 0 1389 1656 Size: 1389 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 292 1656 Size: 292 1364 XbaI T'CTAG_A Cuts at: 0 1074 1656 Size: 1074 582 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 304 1656 Size: 304 1352 XmnI GAAnn'nnTTC Cuts at: 0 95 826 944 1656 Size: 95 731 118 712 Fragments arranged by size: 731 712 118 95 ZraI GAC'GTC Cuts at: 0 1555 1656 Size: 1555 101 Enzymes that do cut: AatII AclI AcuI ApoI BbsI BfrBI BpmI BsaI BsaBI BsaHI BsaXI BseRI BsmI BsmBI BspMI BstBI BstYI BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NsiI NspI PflMI PpiI PvuII SacII SfcI SspI StuI TaqII XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI SwaI TatI TstI Tth111I XhoI XmaI