################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BamHI BbrPI BbvCI BciVI BfrI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI HincII HindII HpaI KpnI MfeI MluI MluNI MroI MscI MunI NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfaNI SfiI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 243 GACGTC GACGT^C AcsI 1085 GAATTC [GA]^AATT[CT] AflIII 1400 ACATGT A^C[GA][CT]GT ApaI 1207 GGGCCC GGGCC^C ApoI 1085 GAATTC [GA]^AATT[CT] AvrII 1773 CCTAGG C^CTAGG BbsI 904 GTCTTC GAAGAC(2/6) BclI 476 TGATCA T^GATCA BglII 524 AGATCT A^GATCT BlnI 1773 CCTAGG C^CTAGG BpuAI 904 GTCTTC GAAGAC(2/6) Bsp1407I 665 TGTACA T^GTACA BspHI 456 TCATGA T^CATGA BspLU11I 1400 ACATGT A^CATGT BsrBI 1263 CCGCTC CCGCTC(-3/-3) BsrGI 665 TGTACA T^GTACA BssHII 367 GCGCGC G^CGCGC BstBI 615 TTCGAA TT^CGAA BstXI 1125 CCAAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 2 TGGCCG [CT]^GGCC[GA] EarI 130 GAAGAG CTCTTC(1/4) EcoRI 1085 GAATTC G^AATTC EcoRV 1075 GATATC GAT^ATC EcoT22I 1034 ATGCAT ATGCA^T FbaI 476 TGATCA T^GATCA HgaI 334 GCGTC GACGC(5/10) HindIII 1779 AAGCTT A^AGCTT Ksp632I 130 GAAGAG CTCTTC(1/4) MseI 1783 TTAA T^TAA NsiI 1034 ATGCAT ATGCA^T NspV 615 TTCGAA TT^CGAA RcaI 456 TCATGA T^CATGA SacI 439 GAGCTC GAGCT^C SfuI 615 TTCGAA TT^CGAA SmaI 385 CCCGGG CCC^GGG SphI 32 GCATGC GCATG^C SspBI 665 TGTACA T^GTACA SspI 551 AATATT AAT^ATT StuI 1269 AGGCCT AGG^CCT Tru9I 1783 TTAA T^TAA XcmI 743 CCAACAATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 385 CCCGGG C^CCGGG XmaI 385 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 896 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 1572 CAGCCCCTG AvaI 385 CCCGGG C^[CT]CG[GA]G 690 CTCGGG BanI 79 GGCGCC G^G[CT][GA]CC 124 GGCGCC BbeI 79 GGCGCC GGCGC^C 124 GGCGCC BfaI 1766 CTAG C^TAG 1774 CTAG BglI 127 GCCGAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 167 GCCGCGGCGGC BlpI 36 GCTAAGC GC^T[ACGT]AGC 1731 GCTTAGC Bpu1102I 36 GCTAAGC GC^T[ACGT]AGC 1731 GCTTAGC BsaBI 474 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 958 GATAGGAATC BseRI 1176 CTCCTC GAGGAG(10/8) 1185 CTCCTC BsgI 1808 CTGCAC GTGCAG(16/14) 27 GTGCAG BsmBI 206 GAGACG CGTCTC(1/5) 833 GAGACG BsmI 883 GCATTC GAATGC(1/-1) 1036 GCATTC BsoBI 385 CCCGGG C^[CT]CG[GA]G 690 CTCGGG BspMI 1727 ACCTGC ACCTGC(4/8) 631 GCAGGT BstAPI 631 GCAGGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1698 GCAGATGTTGC CelII 36 GCTAAGC GC^T[ACGT]AGC 1731 GCTTAGC DraIII 656 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 1673 CACCGAGTG EcoNI 1310 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1711 CCTGTGGAAGG KasI 79 GGCGCC G^GCGCC 124 GGCGCC MaeI 1766 CTAG C^TAG 1774 CTAG MaeII 244 ACGT A^CGT 812 ACGT MaeIII 336 GTCAC ^GT[ACGT]AC 916 GTCAC MamI 474 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 958 GATAGGAATC NarI 79 GGCGCC GG^CGCC 124 GGCGCC NcoI 563 CCATGG C^CATGG 1406 CCATGG NdeI 1031 CATATG CA^TATG 1813 CATATG PstI 162 CTGCAG CTGCA^G 427 CTGCAG PvuII 1222 CAGCTG CAG^CTG 1434 CAGCTG SfcI 162 CTGCAG C^T[GA][CT]AG 427 CTGCAG SfoI 79 GGCGCC GGC^GCC 124 GGCGCC SmlI 43 CTCAAG C^T[CT][GA]AG 1799 CTCAAG TaqI 616 TCGA T^CGA 725 TCGA Tsp45I 336 GTCAC ^GT[GC]AC 916 GTCAC Tsp509I 1086 AATT ^AATT 1638 AATT TthHB8I 616 TCGA T^CGA 725 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI ApaLI AspHI AvaII BanII BbvI BcgI BcnI BmyI BpmI BsaHI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI RsaI SacII Sau3AI Sau96I ScrFI StyI TfiI TseI TspRI XhoII