Restriction map for
pFN21AB9752
(Circular) (Six-base) MAPSORT of: pFN21AB9752.seq Check: 1205 from: 1 to: 6526
pFN21AB9752 6526 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3741 3741
Size: 6526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2252 4336 278
Size: 53 83 186 936 716 2084 2468
Fragments arranged by size:
2468 2084 936 716 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3869 1937
Size: 1932 4594
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2759 2975 3421 4589 5973 6241 1632
Size: 458 669 216 446 1168 1384 268 1917
Fragments arranged by size:
1917 1384 1168 669 458 446 268 216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 3405 5425 6402 1203
Size: 2202 2020 977 1327
Fragments arranged by size:
2202 2020 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 3398 1166
Size: 2232 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3123 1184
Size: 1939 4587
AlwNI CAG_nnn'CTG
Cuts at: 2906 3582 4269 5841 2906
Size: 676 687 1572 3591
Fragments arranged by size:
3591 1572 687 676
ApaI G_GGCC'C
Cuts at: 3216 3216
Size: 6526
ApaLI G'TGCA_C
Cuts at: 2813 3585 3728 4583 5739 2813
Size: 772 143 855 1156 3600
Fragments arranged by size:
3600 1156 855 772 143
ApoI r'AATT_y
Cuts at: 1493 3090 3835 4061 4213 6322 1493
Size: 1597 745 226 152 2109 1697
Fragments arranged by size:
2109 1697 1597 745 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2390 2695 3851 1870
Size: 73 447 305 1156 4545
Fragments arranged by size:
4545 1156 447 305 73
AvrII C'CTAG_G
Cuts at: 3778 3778
Size: 6526
BamHI G'GATC_C
Cuts at: 3856 4223 3856
Size: 367 6159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2129 3847 5302 618
Size: 325 217 627 297 45 1718 1455 1842
Fragments arranged by size:
1842 1718 1455 627 325 297 217 45
BanII G_rGCy'C
Cuts at: 1391 2027 2448 3216 3845 1391
Size: 636 421 768 629 4072
Fragments arranged by size:
4072 768 636 629 421
BbeI G_GCGC'C
Cuts at: 2088 2133 2088
Size: 45 6481
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2902 928
Size: 971 1003 4552
Fragments arranged by size:
4552 1003 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2204 2238 2930 2964 3508 3542 4717
Size: 34 267 34 692 34 544 34 1175
Cuts at: 4717 4751 1903
Size: 34 3678
Fragments arranged by size:
3678 1175 692 544 267 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 2481 1548
Size: 933 5593
BfrBI ATG'CAT
Cuts at: 3041 3041
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2178 5137
Size: 107 122 71 631 605 466 40 2959
Cuts at: 5137 136
Size: 1525
Fragments arranged by size:
2959 1525 631 605 466 122 107 71
40
BglII A'GATC_T
Cuts at: 2529 6521 2529
Size: 3992 2534
BlpI GC'TnA_GC
Cuts at: 2042 3737 3943 2042
Size: 1695 206 4625
Fragments arranged by size:
4625 1695 206
Bme1580I G_kGCm'C
Cuts at: 1790 2817 3216 3268 3589 3732 4587 5743 1790
Size: 1027 399 52 321 143 855 1156 2573
Fragments arranged by size:
2573 1156 1027 855 399 321 143 52
BmgBI CAC'GTC
Cuts at: 1318 2818 1318
Size: 1500 5026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 3147 4257
Size: 416 53 83 399 24 718 1012 1110
Cuts at: 4257 5215 442
Size: 958 1753
Fragments arranged by size:
1753 1110 1012 958 718 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2462 2462
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3476 3499 5186 1239
Size: 1061 1176 23 1687 2579
Fragments arranged by size:
2579 1687 1176 1061 23
Bpu10I CC'TnA_GC
Cuts at: 3271 3271
Size: 6526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 3789 4651 5516 5814 2033
Size: 1756 862 865 298 2745
Fragments arranged by size:
2745 1756 865 862 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2370 3305 3338 5189 882
Size: 617 871 935 33 1851 2219
Fragments arranged by size:
2219 1851 935 871 617 33
BsaAI yAC'GTr
Cuts at: 493 1204 4317 493
Size: 711 3113 2702
Fragments arranged by size:
3113 2702 711
BsaBI GATnn'nnATC
Cuts at: 2483 2967 6520 2483
Size: 484 3553 2489
Fragments arranged by size:
3553 2489 484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2130 2249 2339
Size: 53 83 186 936 552 45 119 90
Cuts at: 2339 4333 4715 5350 275
Size: 1994 382 635 1451
Fragments arranged by size:
1994 1451 936 635 552 382 186 119
90 83 53 45
BsaWI w'CCGG_w
Cuts at: 4959 5357 5631 5778 6189 4959
Size: 398 274 147 411 5296
Fragments arranged by size:
5296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6301
Fragments arranged by size:
6301 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3172 3181 1186
Size: 1986 9 4531
Fragments arranged by size:
4531 1986 9
BseYI C'CCAG_C
Cuts at: 2240 3486 5729 6225 2240
Size: 1246 2243 496 2541
Fragments arranged by size:
2541 2243 1246 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 3798 1163
Size: 573 317 1745 3891
Fragments arranged by size:
3891 1745 573 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3976 4737 5765 1540
Size: 275 186 1975 761 1028 2301
Fragments arranged by size:
2301 1975 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 2817 3589 3732 4587 4672 5743 2448
Size: 369 772 143 855 85 1071 3231
Fragments arranged by size:
3231 1071 855 772 369 143 85
BsmI GAATG_Cn'
Cuts at: 2888 3041 4037 4130 2888
Size: 153 996 93 5284
Fragments arranged by size:
5284 996 153 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2205 2832 2205
Size: 627 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2448 2817 3216 3268 3589 3732
Size: 399 237 421 369 399 52 321 143
Cuts at: 3732 3845 4587 4672 5743 1391
Size: 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 399 369 321
237 143 113 85 52
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 2461 4415 6145 2461
Size: 1954 1730 2842
Fragments arranged by size:
2842 1954 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 3741 3881 844
Size: 1783 1114 140 3489
Fragments arranged by size:
3489 1783 1114 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 4413 1394
Size: 429 1447 1143 3507
Fragments arranged by size:
3507 1447 1143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 2562 3112 3526 5021 5195 65
Size: 1183 1275 39 550 414 1495 174 1396
Fragments arranged by size:
1495 1396 1275 1183 550 414 174 39
BsrFI r'CCGG_y
Cuts at: 1700 1810 2236 2257 2495 5170 5357 1700
Size: 110 426 21 238 2675 187 2869
Fragments arranged by size:
2869 2675 426 238 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 2670 96
Size: 2574 3952
BssHII G'CGCG_C
Cuts at: 1925 2372 1925
Size: 447 6079
BssSI C'ACGA_G
Cuts at: 4586 5598 4586
Size: 1012 5514
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2642 3709 794
Size: 1848 1067 3611
Fragments arranged by size:
3611 1848 1067
BstBI TT'CG_AA
Cuts at: 2621 5330 2621
Size: 2709 3817
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6526
BstXI CCAn_nnnn'nTGG
Cuts at: 3137 3137
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2428 2529 2542 3856 4223 4608 4625 6077
Size: 456 101 13 1314 367 385 17 1452
Cuts at: 6077 6163 6175 6521 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1314 456 385 367 346 101
86 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2173 2292 2420 2568 3411
Size: 552 939 147 22 119 128 148 843
Cuts at: 3411 513
Size: 3628
Fragments arranged by size:
3628 939 843 552 148 147 128 119
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5787
Fragments arranged by size:
5787 683 56
BtsI GCAGTG_nn'
Cuts at: 2922 3748 4042 4836 4856 2922
Size: 826 294 794 20 4592
Fragments arranged by size:
4592 826 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DraIII CAC_nnn'GTG
Cuts at: 2666 3683 2666
Size: 1017 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3973 4862
Size: 54 1425 50 275 18 177 1966 889
Cuts at: 4862 6491 8
Size: 1629 43
Fragments arranged by size:
1966 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 4456 1353
Size: 777 2326 3423
Fragments arranged by size:
3423 2326 777
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5113 5487 5633 387
Size: 1064 138 3524 374 146 1280
Fragments arranged by size:
3524 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2446 2446
Size: 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2300 2759 2975 3421 3476 3499
Size: 393 458 210 459 216 446 55 23
Cuts at: 3499 4589 5186 5973 6241 1239
Size: 1090 597 787 268 1524
Fragments arranged by size:
1524 1090 787 597 459 458 446 393
268 216 210 55 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3319 3720 3319
Size: 401 6125
EcoO109I rG'GnC_Cy
Cuts at: 2394 2416 3212 3672 3774 2394
Size: 22 796 460 102 5146
Fragments arranged by size:
5146 796 460 102 22
EcoRI G'AATT_C
Cuts at: 3090 3835 4213 3090
Size: 745 378 5403
Fragments arranged by size:
5403 745 378
EcoRV GAT'ATC
Cuts at: 3082 3082
Size: 6526
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 2078 2088 2133 2223 5673 1678
Size: 400 10 45 90 3450 2531
Fragments arranged by size:
3450 2531 400 90 45 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 2706 2738 2802
Size: 32 135 32 942 32 160 32 64
Cuts at: 2802 2834 5167 5199 5241 5273 1373
Size: 32 2333 32 42 32 2626
Fragments arranged by size:
2626 2333 942 160 135 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3870 4116 669
Size: 988 281 1932 246 3079
Fragments arranged by size:
3079 1932 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3784 748
Size: 3036 3490
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 2084 2129 2084
Size: 45 6481
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5640 5824 1305
Size: 4335 184 2007
Fragments arranged by size:
4335 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4704
Fragments arranged by size:
4704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2567 3398 3410 4484 4843 5002
Size: 648 1311 90 831 12 1074 359 159
Cuts at: 5002 518
Size: 2042
Fragments arranged by size:
2042 1311 1074 831 648 359 159 90
12
MspA1I CmG'CkG
Cuts at: 1606 2175 2294 2422 3229 3441 3942 4619 5767
Size: 569 119 128 807 212 501 677 1148
Cuts at: 5767 6012 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 807 677 569 501 245 212
128 119
NaeI GCC'GGC
Cuts at: 2238 2259 2497 2238
Size: 21 238 6267
Fragments arranged by size:
6267 238 21
NarI GG'CG_CC
Cuts at: 2085 2130 2085
Size: 45 6481
NcoI C'CATG_G
Cuts at: 513 1065 2004 2568 3411 513
Size: 552 939 564 843 3628
Fragments arranged by size:
3628 939 843 564 552
NdeI CA'TA_TG
Cuts at: 387 3037 3819 387
Size: 2650 782 3094
Fragments arranged by size:
3094 2650 782
NgoMIV G'CCGG_C
Cuts at: 2236 2257 2495 2236
Size: 21 238 6267
Fragments arranged by size:
6267 238 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NsiI A_TGCA'T
Cuts at: 3043 3043
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 2041 2509 3409 3884 5429 1132
Size: 97 812 468 900 475 1545 2229
Fragments arranged by size:
2229 1545 900 812 475 468 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 3405 5425 3405
Size: 2020 4506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6526
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 3672 3774 2394
Size: 1278 102 5146
Fragments arranged by size:
5146 1278 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 3212 3212
Size: 6526
PstI C_TGCA'G
Cuts at: 830 2171 2436 3878 830
Size: 1341 265 1442 3478
Fragments arranged by size:
3478 1442 1341 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 3229 3441 3229
Size: 212 6314
SacI G_AGCT'C
Cuts at: 2448 2448
Size: 6526
SacII CC_GC'GG
Cuts at: 2176 2295 2423 2176
Size: 119 128 6279
Fragments arranged by size:
6279 128 119
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SanDI GG'GwC_CC
Cuts at: 2394 2394
Size: 6526
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2167 2432 3874 5009 5690 5881 826
Size: 219 1122 265 1442 1135 681 191 1471
Fragments arranged by size:
1471 1442 1135 1122 681 265 219 191
SfoI GGC'GCC
Cuts at: 2086 2131 2086
Size: 45 6481
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2392 3853 1872
Size: 520 1461 4545
Fragments arranged by size:
4545 1461 520
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 3804 4630 5531 5793 820
Size: 197 926 105 1756 826 901 262 1553
Fragments arranged by size:
1756 1553 926 901 826 262 197 105
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 2041 3884 1132
Size: 909 1843 3774
Fragments arranged by size:
3774 1843 909
SspI AAT'ATT
Cuts at: 5 52 2558 4450 5
Size: 47 2506 1892 2081
Fragments arranged by size:
2506 2081 1892 47
StuI AGG'CCT
Cuts at: 1517 3276 1517
Size: 1759 4767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2490 2568 2767 3024 3130
Size: 552 939 354 132 78 199 257 106
Cuts at: 3130 3411 3655 3778 3965 513
Size: 281 244 123 187 3074
Fragments arranged by size:
3074 939 552 354 281 257 244 199
187 132 123 106 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 4553 4570 4723 4908 5353 325
Size: 1255 1824 1149 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 1149 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 4772 6484
Size: 274 80 33 51 1443 693 2102 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
2102 1712 1443 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6486 6518 6486
Size: 32 6494
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 2390 3851 1870
Size: 520 1461 4545
Fragments arranged by size:
4545 1461 520
XmnI GAAnn'nnTTC
Cuts at: 4655 4655
Size: 6526
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2250 4334 276
Size: 53 83 186 936 716 2084 2468
Fragments arranged by size:
2468 2084 936 716 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BsiWI BstZ17I Bsu36I
FalI FseI FspAI NruI PacI PfoI PmlI PspXI
PsrI RsrII SapI SexAI SfiI SrfI SwaI