(Linear) (Six-base) MAPSORT of: KIBB9752.seq Check: 4088 from: 1 to: 1821 KIBB9752.orf With 182 enzymes: SgfI * July 3, 2012 16:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1736 1821 Size: 1736 85 AatII G_ACGT'C Cuts at: 0 247 1821 Size: 247 1574 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 754 970 1416 1821 Size: 85 669 216 446 405 Fragments arranged by size: 669 446 405 216 85 AflIII A'CryG_T Cuts at: 0 1400 1821 Size: 1400 421 AleI CACnn'nnGTG Cuts at: 0 1393 1821 Size: 1393 428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1118 1821 Size: 1118 703 AlwNI CAG_nnn'CTG Cuts at: 0 901 1577 1821 Size: 901 676 244 Fragments arranged by size: 901 676 244 ApaI G_GGCC'C Cuts at: 0 1211 1821 Size: 1211 610 ApaLI G'TGCA_C Cuts at: 0 808 1580 1723 1821 Size: 808 772 143 98 Fragments arranged by size: 808 772 143 98 ApoI r'AATT_y Cuts at: 0 1085 1821 Size: 1085 736 AvaI C'yCGr_G Cuts at: 0 385 690 1821 Size: 385 305 1131 Fragments arranged by size: 1131 385 305 AvrII C'CTAG_G Cuts at: 0 1773 1821 Size: 1773 48 BanI G'GyrC_C Cuts at: 0 79 124 1821 Size: 79 45 1697 Fragments arranged by size: 1697 79 45 BanII G_rGCy'C Cuts at: 0 22 443 1211 1821 Size: 22 421 768 610 Fragments arranged by size: 768 610 421 22 BbeI G_GCGC'C Cuts at: 0 83 128 1821 Size: 83 45 1693 Fragments arranged by size: 1693 83 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 897 1821 Size: 897 924 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 233 925 959 1503 1537 1821 Size: 199 34 692 34 544 34 284 Fragments arranged by size: 692 544 284 199 34 34 34 BclI T'GATC_A Cuts at: 0 476 1821 Size: 476 1345 BfrBI ATG'CAT Cuts at: 0 1036 1821 Size: 1036 785 BglI GCCn_nnn'nGGC Cuts at: 0 133 173 1821 Size: 133 40 1648 Fragments arranged by size: 1648 133 40 BglII A'GATC_T Cuts at: 0 524 1821 Size: 524 1297 BlpI GC'TnA_GC Cuts at: 0 37 1732 1821 Size: 37 1695 89 Fragments arranged by size: 1695 89 37 Bme1580I G_kGCm'C Cuts at: 0 812 1211 1263 1584 1727 1821 Size: 812 399 52 321 143 94 Fragments arranged by size: 812 399 321 143 94 52 BmgBI CAC'GTC Cuts at: 0 813 1821 Size: 813 1008 BmrI ACTGGGnnnn_n' Cuts at: 0 130 1142 1821 Size: 130 1012 679 Fragments arranged by size: 1012 679 130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 457 1821 Size: 457 1364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1471 1494 1821 Size: 295 1176 23 327 Fragments arranged by size: 1176 327 295 23 Bpu10I CC'TnA_GC Cuts at: 0 1266 1821 Size: 1266 555 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 1784 1821 Size: 28 1756 37 Fragments arranged by size: 1756 37 28 BsaI GGTCTCn'nnnn_ Cuts at: 0 365 1300 1333 1821 Size: 365 935 33 488 Fragments arranged by size: 935 488 365 33 BsaBI GATnn'nnATC Cuts at: 0 478 962 1821 Size: 478 484 859 Fragments arranged by size: 859 484 478 BsaHI Gr'CG_yC Cuts at: 0 80 125 244 334 1821 Size: 80 45 119 90 1487 Fragments arranged by size: 1487 119 90 80 45 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1167 1176 1821 Size: 1167 9 645 Fragments arranged by size: 1167 645 9 BseYI C'CCAG_C Cuts at: 0 235 1481 1821 Size: 235 1246 340 Fragments arranged by size: 1246 340 235 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 1793 1821 Size: 48 1745 28 Fragments arranged by size: 1745 48 28 BsiHKAI G_wGCw'C Cuts at: 0 443 812 1584 1727 1821 Size: 443 369 772 143 94 Fragments arranged by size: 772 443 369 143 94 BsmI GAATG_Cn' Cuts at: 0 883 1036 1821 Size: 883 153 785 Fragments arranged by size: 883 785 153 BsmBI CGTCTCn'nnnn_ Cuts at: 0 200 827 1821 Size: 200 627 994 Fragments arranged by size: 994 627 200 Bsp1286I G_dGCh'C Cuts at: 0 22 443 812 1211 1263 1584 1727 1821 Size: 22 421 369 399 52 321 143 94 Fragments arranged by size: 421 399 369 321 143 94 52 22 BspHI T'CATG_A Cuts at: 0 456 1821 Size: 456 1365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 622 1736 1821 Size: 622 1114 85 Fragments arranged by size: 1114 622 85 BsrBI CCG'CTC Cuts at: 0 1265 1821 Size: 1265 556 BsrDI GCAATG_nn' Cuts at: 0 518 557 1107 1521 1821 Size: 518 39 550 414 300 Fragments arranged by size: 550 518 414 300 39 BsrFI r'CCGG_y Cuts at: 0 231 252 490 1821 Size: 231 21 238 1331 Fragments arranged by size: 1331 238 231 21 BsrGI T'GTAC_A Cuts at: 0 665 1821 Size: 665 1156 BssHII G'CGCG_C Cuts at: 0 367 1821 Size: 367 1454 BstAPI GCAn_nnn'nTGC Cuts at: 0 637 1704 1821 Size: 637 1067 117 Fragments arranged by size: 1067 637 117 BstBI TT'CG_AA Cuts at: 0 616 1821 Size: 616 1205 BstXI CCAn_nnnn'nTGG Cuts at: 0 1132 1821 Size: 1132 689 BstYI r'GATC_y Cuts at: 0 423 524 537 1821 Size: 423 101 13 1284 Fragments arranged by size: 1284 423 101 13 BtgI C'CryG_G Cuts at: 0 146 168 287 415 563 1406 1821 Size: 146 22 119 128 148 843 415 Fragments arranged by size: 843 415 148 146 128 119 22 BtsI GCAGTG_nn' Cuts at: 0 917 1743 1821 Size: 917 826 78 Fragments arranged by size: 917 826 78 DraIII CAC_nnn'GTG Cuts at: 0 661 1678 1821 Size: 661 1017 143 Fragments arranged by size: 1017 661 143 EaeI y'GGCC_r Cuts at: 0 2 1821 Size: 2 1819 EarI CTCTTCn'nnn_ Cuts at: 0 125 1821 Size: 125 1696 EcoICRI GAG'CTC Cuts at: 0 441 1821 Size: 441 1380 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 295 754 970 1416 1471 1494 1821 Size: 85 210 459 216 446 55 23 327 Fragments arranged by size: 459 446 327 216 210 85 55 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 1314 1715 1821 Size: 1314 401 106 Fragments arranged by size: 1314 401 106 EcoO109I rG'GnC_Cy Cuts at: 0 389 411 1207 1667 1769 1821 Size: 389 22 796 460 102 52 Fragments arranged by size: 796 460 389 102 52 22 EcoRI G'AATT_C Cuts at: 0 1085 1821 Size: 1085 736 EcoRV GAT'ATC Cuts at: 0 1077 1821 Size: 1077 744 HaeII r_GCGC'y Cuts at: 0 73 83 128 218 1821 Size: 73 10 45 90 1603 Fragments arranged by size: 1603 90 73 45 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 509 541 701 733 797 829 1821 Size: 509 32 160 32 64 32 992 Fragments arranged by size: 992 509 160 64 32 32 32 HindIII A'AGCT_T Cuts at: 0 1779 1821 Size: 1779 42 KasI G'GCGC_C Cuts at: 0 79 124 1821 Size: 79 45 1697 Fragments arranged by size: 1697 79 45 MslI CAynn'nnrTG Cuts at: 0 472 562 1393 1405 1821 Size: 472 90 831 12 416 Fragments arranged by size: 831 472 416 90 12 MspA1I CmG'CkG Cuts at: 0 170 289 417 1224 1436 1821 Size: 170 119 128 807 212 385 Fragments arranged by size: 807 385 212 170 128 119 NaeI GCC'GGC Cuts at: 0 233 254 492 1821 Size: 233 21 238 1329 Fragments arranged by size: 1329 238 233 21 NarI GG'CG_CC Cuts at: 0 80 125 1821 Size: 80 45 1696 Fragments arranged by size: 1696 80 45 NcoI C'CATG_G Cuts at: 0 563 1406 1821 Size: 563 843 415 Fragments arranged by size: 843 563 415 NdeI CA'TA_TG Cuts at: 0 1032 1814 1821 Size: 1032 782 7 Fragments arranged by size: 1032 782 7 NgoMIV G'CCGG_C Cuts at: 0 231 252 490 1821 Size: 231 21 238 1331 Fragments arranged by size: 1331 238 231 21 NsiI A_TGCA'T Cuts at: 0 1038 1821 Size: 1038 783 NspI r_CATG'y Cuts at: 0 36 504 1404 1821 Size: 36 468 900 417 Fragments arranged by size: 900 468 417 36 PciI A'CATG_T Cuts at: 0 1400 1821 Size: 1400 421 PpuMI rG'GwC_Cy Cuts at: 0 389 1667 1769 1821 Size: 389 1278 102 52 Fragments arranged by size: 1278 389 102 52 PspOMI G'GGCC_C Cuts at: 0 1207 1821 Size: 1207 614 PstI C_TGCA'G Cuts at: 0 166 431 1821 Size: 166 265 1390 Fragments arranged by size: 1390 265 166 PvuII CAG'CTG Cuts at: 0 1224 1436 1821 Size: 1224 212 385 Fragments arranged by size: 1224 385 212 SacI G_AGCT'C Cuts at: 0 443 1821 Size: 443 1378 SacII CC_GC'GG Cuts at: 0 171 290 418 1821 Size: 171 119 128 1403 Fragments arranged by size: 1403 171 128 119 SanDI GG'GwC_CC Cuts at: 0 389 1821 Size: 389 1432 SfcI C'TryA_G Cuts at: 0 162 427 1821 Size: 162 265 1394 Fragments arranged by size: 1394 265 162 SfoI GGC'GCC Cuts at: 0 81 126 1821 Size: 81 45 1695 Fragments arranged by size: 1695 81 45 SmaI CCC'GGG Cuts at: 0 387 1821 Size: 387 1434 SmlI C'TyrA_G Cuts at: 0 43 1799 1821 Size: 43 1756 22 Fragments arranged by size: 1756 43 22 SphI G_CATG'C Cuts at: 0 36 1821 Size: 36 1785 SspI AAT'ATT Cuts at: 0 553 1821 Size: 553 1268 StuI AGG'CCT Cuts at: 0 1271 1821 Size: 1271 550 StyI C'CwwG_G Cuts at: 0 353 485 563 762 1019 1125 1406 1650 Size: 353 132 78 199 257 106 281 244 Cuts at: 1650 1773 1821 Size: 123 48 Fragments arranged by size: 353 281 257 244 199 132 123 106 78 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1399 1821 Size: 1399 422 TatI w'GTAC_w Cuts at: 0 665 1821 Size: 665 1156 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 750 1821 Size: 750 1071 XmaI C'CCGG_G Cuts at: 0 385 1821 Size: 385 1436 ZraI GAC'GTC Cuts at: 0 245 1821 Size: 245 1576 Enzymes that do cut: AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HindIII KasI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BamHI BbvCI BciVI BmtI BsaAI BsaWI BsaXI BsiEI BsiWI BspEI BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI MscI NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XhoI XmnI