################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BanI BbeI BciVI BclI BfrI BglII BlnI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 565 GTAGAC GT^[AC][GT]AC AcyI 2338 GACGCC G[GA]^CG[CT]C AflIII 254 ACACGT A^C[GA][CT]GT AocI 631 CCTGAGG CC^T[ACGT]AGG BalI 113 TGGCCA TGG^CCA BamHI 260 GGATCC G^GATCC BbrPI 255 CACGTG CAC^GTG BbsI 1360 GAAGAC GAAGAC(2/6) BbvCI 87 CCTCAGC CCTCAGC(-5/-2) BcgI 464 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1360 GAAGAC GAAGAC(2/6) BsaAI 255 CACGTG [CT]AC^GT[GA] BsaBI 1134 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2338 GACGCC G[GA]^CG[CT]C BsaI 1381 GAGACC GGTCTC(1/5) BspHI 545 TCATGA T^CATGA BsrBI 2299 GAGCGG CCGCTC(-3/-3) BsrDI 1989 CATTGC GCAATG(2/0) BssSI 493 CACGAG CACGAG(-5/-1) Bsu36I 631 CCTGAGG CC^T[ACGT]AGG Eco81I 631 CCTGAGG CC^T[ACGT]AGG EcoT14I 2309 CCAAGG C^C[AT][AT]GG FseI 2303 GGCCGGCC GGCCGG^CC Hin1I 2338 GACGCC G[GA]^CG[CT]C HindIII 792 AAGCTT A^AGCTT KspI 1623 CCGCGG CCGC^GG MaeIII 1207 GTGAC ^GT[ACGT]AC MamI 1134 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 113 TGGCCA TGG^CCA MscI 113 TGGCCA TGG^CCA MseI 1574 TTAA T^TAA NaeI 2304 GCCGGC GCC^GGC NgoMIV 2304 GCCGGC G^CCGGC NotI 1628 GCGGCCGC GC^GGCCGC PmaCI 255 CACGTG CAC^GTG PmlI 255 CACGTG CAC^GTG RcaI 545 TCATGA T^CATGA SacII 1623 CCGCGG CCGC^GG SbfI 2241 CCTGCAGG CCTGCA^GG SexAI 1052 ACCAGGT A^CC[AT]GGT SmaI 869 CCCGGG CCC^GGG Sse8387I 2241 CCTGCAGG CCTGCA^GG StuI 641 AGGCCT AGG^CCT StyI 2309 CCAAGG C^C[AT][AT]GG Tru9I 1574 TTAA T^TAA Tsp45I 1207 GTGAC ^GT[GC]AC XmaCI 869 CCCGGG C^CCGGG XmaI 869 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AhdI 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1363 GACTTCGTGTC ApaI 1427 GGGCCC GGGCC^C 2121 GGGCCC AspEI 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1363 GACTTCGTGTC AvaI 715 CTCGGG C^[CT]CG[GA]G 869 CCCGGG BfaI 205 CTAG C^TAG 1192 CTAG BglI 43 GCCCTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1612 GCCCATCTGGC BlpI 1872 GCTCAGC GC^T[ACGT]AGC 2160 GCTGAGC Bpu1102I 1872 GCTCAGC GC^T[ACGT]AGC 2160 GCTGAGC BsmBI 1411 GAGACG CGTCTC(1/5) 1759 GAGACG BsoBI 715 CTCGGG C^[CT]CG[GA]G 869 CCCGGG BssHII 1809 GCGCGC G^CGCGC 2278 GCGCGC BstYI 260 GGATCC [GA]^GATC[CT] 2003 AGATCC CelII 1872 GCTCAGC GC^T[ACGT]AGC 2160 GCTGAGC DrdI 397 GACATGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1030 GACAAGCTGGTC Eam1105I 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1363 GACTTCGTGTC HphI 939 GGTGA GGTGA(8/7) 1926 GGTGA MaeI 205 CTAG C^TAG 1192 CTAG MflI 260 GGATCC [GA]^GATC[CT] 2003 AGATCC NspI 398 ACATGC [GA]CATG^[CT] 743 ACATGC ScaI 416 AGTACT AGT^ACT 986 AGTACT SfiI 42 GGCCCTGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1611 GGCCCATCTGGCC XhoII 260 GGATCC [GA]^GATC[CT] 2003 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI TspRI TthHB8I Van91I