(Circular) (Six-base) MAPSORT of: pFN21AE4225.seq Check: 3623 from: 1 to: 7087 pFN21AE4225 7087 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2072 2932 2072 Size: 860 6227 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4897 278 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2571 4430 1937 Size: 634 1859 4594 Fragments arranged by size: 4594 1859 634 Acc65I G'GTAC_C Cuts at: 4408 4408 Size: 7087 AclI AA'CG_TT Cuts at: 1563 4386 5214 5587 1563 Size: 2823 828 373 3063 Fragments arranged by size: 3063 2823 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4121 4142 5150 6534 6802 1632 Size: 1916 573 21 1008 1384 268 1917 Fragments arranged by size: 1917 1916 1384 1008 573 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6890 AflIII A'CryG_T Cuts at: 1203 2259 5986 6963 1203 Size: 1056 3727 977 1327 Fragments arranged by size: 3727 1327 1056 977 AgeI A'CCGG_T Cuts at: 5918 5918 Size: 7087 AhdI GACnn_n'nnGTC Cuts at: 2161 3373 5816 2161 Size: 1212 2443 3432 Fragments arranged by size: 3432 2443 1212 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4011 1184 Size: 2827 4260 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2186 2218 2408 2440 2486 2518 2900 2932 2186 Size: 32 190 32 46 32 382 32 6341 Fragments arranged by size: 6341 382 190 46 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2386 3721 3844 4378 4830 6402 2386 Size: 1335 123 534 452 1572 3071 Fragments arranged by size: 3071 1572 1335 534 452 123 ApaI G_GGCC'C Cuts at: 3436 4130 3436 Size: 694 6393 ApaLI G'TGCA_C Cuts at: 5144 6300 5144 Size: 1156 5931 ApoI r'AATT_y Cuts at: 1493 4396 4622 4774 6883 1493 Size: 2903 226 152 2109 1697 Fragments arranged by size: 2903 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5641 160 Size: 872 4609 1606 Fragments arranged by size: 4609 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 AvaI C'yCGr_G Cuts at: 1870 1943 2720 2874 4412 1870 Size: 73 777 154 1538 4545 Fragments arranged by size: 4545 1538 777 154 73 BamHI G'GATC_C Cuts at: 2265 4417 4784 2265 Size: 2152 367 4568 Fragments arranged by size: 4568 2152 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4408 5863 618 Size: 325 217 627 2621 1455 1842 Fragments arranged by size: 2621 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2027 2697 2721 2727 3436 3600 4080 Size: 624 12 670 24 6 709 164 480 Cuts at: 4080 4130 4406 1391 Size: 50 276 4072 Fragments arranged by size: 4072 709 670 624 480 276 164 50 24 12 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3372 928 Size: 971 1473 4643 Fragments arranged by size: 4643 1473 971 BbvCI CC'TCA_GC Cuts at: 2093 2093 Size: 7087 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2458 2492 5278 5312 1903 Size: 34 521 34 2786 34 3678 Fragments arranged by size: 3678 2786 521 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4979 6189 4979 Size: 1210 5877 BclI T'GATC_A Cuts at: 1548 1548 Size: 7087 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 3623 5698 Size: 107 122 71 631 605 382 1569 2075 Cuts at: 5698 136 Size: 1525 Fragments arranged by size: 2075 1569 1525 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 7082 7082 Size: 7087 BlpI GC'TnA_GC Cuts at: 3878 4166 4504 3878 Size: 288 338 6461 Fragments arranged by size: 6461 338 288 Bme1580I G_kGCm'C Cuts at: 1790 3436 4130 5148 6304 1790 Size: 1646 694 1018 1156 2573 Fragments arranged by size: 2573 1646 1156 1018 694 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7087 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2983 3437 4818 Size: 416 53 83 399 24 1566 454 1381 Cuts at: 4818 5776 442 Size: 958 1753 Fragments arranged by size: 1753 1566 1381 958 454 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7087 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2034 2173 2945 4348 2034 Size: 139 772 1403 4773 Fragments arranged by size: 4773 1403 772 139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2168 2270 2927 3113 3404 3416 4025 Size: 890 39 102 657 186 291 12 609 Cuts at: 4025 4322 5747 1239 Size: 297 1425 2579 Fragments arranged by size: 2579 1425 890 657 609 297 291 186 102 39 12 Bpu10I CC'TnA_GC Cuts at: 2093 2240 2093 Size: 147 6940 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2312 2420 3521 3782 4064 5212 6077 6375 2312 Size: 108 1101 261 282 1148 865 298 3024 Fragments arranged by size: 3024 1148 1101 865 298 282 261 108 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3380 5750 882 Size: 617 1881 2370 2219 Fragments arranged by size: 2370 2219 1881 617 BsaAI yAC'GTr Cuts at: 493 1204 2262 4878 493 Size: 711 1058 2616 2702 Fragments arranged by size: 2702 2616 1058 711 BsaBI GATnn'nnATC Cuts at: 3143 7081 3143 Size: 3938 3149 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4344 4894 5276 5911 Size: 53 83 186 936 2811 550 382 635 Cuts at: 5911 275 Size: 1451 Fragments arranged by size: 2811 1451 936 635 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5520 5918 6192 6339 6750 5520 Size: 398 274 147 411 5857 Fragments arranged by size: 5857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2186 2216 2246 Size: 30 165 30 633 30 120 30 30 Cuts at: 2246 2276 2408 2438 2900 2930 2969 2999 3086 Size: 30 132 30 462 30 39 30 87 Cuts at: 3086 3116 1178 Size: 30 5149 Fragments arranged by size: 5149 633 462 165 132 120 87 39 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2194 2212 2221 2267 2312 2612 2654 Size: 837 171 18 9 46 45 300 42 Cuts at: 2654 2657 3146 3248 3536 3941 4109 4229 1186 Size: 3 489 102 288 405 168 120 4044 Fragments arranged by size: 4044 837 489 405 300 288 171 168 120 102 46 45 42 18 9 3 BseYI C'CCAG_C Cuts at: 2705 6290 6786 2705 Size: 3585 496 3006 Fragments arranged by size: 3585 3006 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2410 3301 3834 1163 Size: 573 674 891 533 4416 Fragments arranged by size: 4416 891 674 573 533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3294 3637 4310 4537 5298 6326 Size: 275 186 1293 343 673 227 761 1028 Cuts at: 6326 1540 Size: 2301 Fragments arranged by size: 2301 1293 1028 761 673 343 275 227 186 BsiHKAI G_wGCw'C Cuts at: 2697 2721 3320 3600 4080 5148 5233 6304 2697 Size: 24 599 280 480 1068 85 1071 3480 Fragments arranged by size: 3480 1071 1068 599 480 280 85 24 BsmI GAATG_Cn' Cuts at: 4598 4691 4598 Size: 93 6994 BsmBI CGTCTCn'nnnn_ Cuts at: 3410 3758 3410 Size: 348 6739 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2027 2697 2721 2727 3320 3436 Size: 399 225 12 670 24 6 593 116 Cuts at: 3436 3600 4080 4130 4406 5148 5233 6304 1391 Size: 164 480 50 276 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 593 480 399 276 225 164 116 85 50 24 12 6 BspEI T'CCGG_A Cuts at: 6750 6750 Size: 7087 BspHI T'CATG_A Cuts at: 2550 4976 6706 2550 Size: 2426 1730 2931 Fragments arranged by size: 2931 2426 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2932 3309 4442 844 Size: 1228 860 377 1133 3489 Fragments arranged by size: 3489 1228 1133 860 377 BsrBI CCG'CTC Cuts at: 1394 1823 4306 4974 1394 Size: 429 2483 668 3507 Fragments arranged by size: 3507 2483 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3993 5582 5756 65 Size: 1183 2745 1589 174 1396 Fragments arranged by size: 2745 1589 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 2682 3168 4309 5731 5918 1700 Size: 110 329 543 486 1141 1422 187 2869 Fragments arranged by size: 2869 1422 1141 543 486 329 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7087 BssHII G'CGCG_C Cuts at: 1925 3814 4283 1925 Size: 1889 469 4729 Fragments arranged by size: 4729 1889 469 BssSI C'ACGA_G Cuts at: 2498 5147 6159 2498 Size: 2649 1012 3426 Fragments arranged by size: 3426 2649 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7087 BstBI TT'CG_AA Cuts at: 5891 5891 Size: 7087 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7087 BstYI r'GATC_y Cuts at: 1972 2265 4008 4417 4784 5169 5186 6638 6724 Size: 293 1743 409 367 385 17 1452 86 Cuts at: 6724 6736 7082 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1743 1452 409 385 367 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 7087 BtgI C'CryG_G Cuts at: 513 1065 2004 2397 3628 3653 513 Size: 552 939 393 1231 25 3947 Fragments arranged by size: 3947 1231 939 552 393 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2461 4044 4293 487 Size: 683 56 1235 1583 249 3281 Fragments arranged by size: 3281 1583 1235 683 249 56 BtsI GCAGTG_nn' Cuts at: 3284 4603 5397 5417 3284 Size: 1319 794 20 4954 Fragments arranged by size: 4954 1319 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7087 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7052 DraI TTT'AAA Cuts at: 4391 4391 Size: 7087 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2408 3041 6094 809 Size: 507 1092 633 3053 1802 Fragments arranged by size: 3053 1802 1092 633 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2712 3291 Size: 54 1425 50 275 18 288 594 579 Cuts at: 3291 3634 3723 3975 4307 4311 4356 4534 5423 Size: 343 89 252 332 4 45 178 889 Cuts at: 5423 7052 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 594 579 343 332 288 275 252 178 89 54 50 45 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3291 3634 4307 4534 1537 Size: 275 1479 343 673 227 4090 Fragments arranged by size: 4090 1479 673 343 275 227 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 2546 2702 3080 3110 5017 1353 Size: 771 422 156 378 30 1907 3423 Fragments arranged by size: 3423 1907 771 422 378 156 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2206 2969 3245 3824 3971 4283 Size: 1064 138 617 763 276 579 147 312 Cuts at: 4283 5674 6048 6194 387 Size: 1391 374 146 1280 Fragments arranged by size: 1391 1280 1064 763 617 579 374 312 276 147 146 138 EcoICRI GAG'CTC Cuts at: 2695 2719 3598 4078 2695 Size: 24 879 480 5704 Fragments arranged by size: 5704 879 480 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2168 2270 2927 3113 3404 3416 Size: 393 497 39 102 657 186 291 12 Cuts at: 3416 3548 4025 4121 4142 4322 5150 5747 6534 Size: 132 477 96 21 180 828 597 787 Cuts at: 6534 6802 1239 Size: 268 1524 Fragments arranged by size: 1524 828 787 657 597 497 477 393 291 268 186 180 132 102 96 39 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2528 2939 3200 3305 3725 2252 Size: 276 411 261 105 420 5614 Fragments arranged by size: 5614 420 411 276 261 105 EcoO109I rG'GnC_Cy Cuts at: 2047 2536 3298 3432 3433 3718 4126 4144 2047 Size: 489 762 134 1 285 408 18 4990 Fragments arranged by size: 4990 762 489 408 285 134 18 1 EcoRI G'AATT_C Cuts at: 4396 4774 4396 Size: 378 6709 FseI GG_CCGG'CC Cuts at: 4313 4313 Size: 7087 FspI TGC'GCA Cuts at: 5593 5593 Size: 7087 HaeII r_GCGC'y Cuts at: 1678 2397 2604 3536 3824 4242 6234 1678 Size: 719 207 932 288 418 1992 2531 Fragments arranged by size: 2531 1992 932 719 418 288 207 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2036 2053 2068 2147 Size: 32 135 32 449 15 17 15 79 Cuts at: 2147 2179 2969 3001 3359 3391 3685 3717 4178 Size: 32 790 32 358 32 294 32 461 Cuts at: 4178 4210 5728 5760 5802 5834 1373 Size: 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 790 461 449 358 294 135 79 42 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 4431 4677 669 Size: 988 281 2493 246 3079 Fragments arranged by size: 3079 2493 988 281 246 HindIII A'AGCT_T Cuts at: 748 2797 748 Size: 2049 5038 HpaI GTT'AAC Cuts at: 4677 4677 Size: 7087 KpnI G_GTAC'C Cuts at: 4412 4412 Size: 7087 MfeI C'AATT_G Cuts at: 4686 4686 Size: 7087 MluI A'CGCG_T Cuts at: 6963 6963 Size: 7087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 4188 6201 6385 1305 Size: 954 1929 2013 184 2007 Fragments arranged by size: 2013 2007 1929 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 4977 Fragments arranged by size: 4977 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2630 2810 2891 5045 5404 5563 Size: 648 1236 228 180 81 2154 359 159 Cuts at: 5563 518 Size: 2042 Fragments arranged by size: 2154 2042 1236 648 359 228 180 159 81 MspA1I CmG'CkG Cuts at: 1606 2968 3027 3184 3514 3589 3630 3639 4042 Size: 1362 59 157 330 75 41 9 403 Cuts at: 4042 4150 4503 5180 6328 6573 1606 Size: 108 353 677 1148 245 2120 Fragments arranged by size: 2120 1362 1148 677 403 353 330 245 157 108 75 59 41 9 NaeI GCC'GGC Cuts at: 4311 4311 Size: 7087 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5596 Fragments arranged by size: 5596 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7087 NgoMIV G'CCGG_C Cuts at: 4309 4309 Size: 7087 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7087 NotI GC'GGCC_GC Cuts at: 3634 4534 3634 Size: 900 6187 NspI r_CATG'y Cuts at: 1132 1229 2407 2752 4445 5990 1132 Size: 97 1178 345 1693 1545 2229 Fragments arranged by size: 2229 1693 1545 1178 345 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7087 PciI A'CATG_T Cuts at: 5986 5986 Size: 7087 PflMI CCAn_nnn'nTGG Cuts at: 1108 2292 2376 3559 3721 4378 1108 Size: 1184 84 1183 162 657 3817 Fragments arranged by size: 3817 1184 1183 657 162 84 PfoI T'CCnGG_A Cuts at: 2145 2145 Size: 7087 PmeI GTTT'AAAC Cuts at: 4391 4391 Size: 7087 PmlI CAC'GTG Cuts at: 2262 2262 Size: 7087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2186 2218 2408 2440 2900 2932 5156 5188 6669 Size: 32 190 32 460 32 2224 32 1481 Cuts at: 6669 6701 2186 Size: 32 2572 Fragments arranged by size: 2572 2224 1481 460 190 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2536 3298 4144 2536 Size: 762 846 5479 Fragments arranged by size: 5479 846 762 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7087 PsiI TTA'TAA Cuts at: 4657 4657 Size: 7087 PspOMI G'GGCC_C Cuts at: 3432 4126 3432 Size: 694 6393 PstI C_TGCA'G Cuts at: 830 2757 2943 3306 3519 3588 3594 3681 3828 Size: 1927 186 363 213 69 6 87 147 Cuts at: 3828 3840 3870 4251 4439 830 Size: 12 30 381 188 3478 Fragments arranged by size: 3478 1927 381 363 213 188 186 147 87 69 30 12 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7087 PvuII CAG'CTG Cuts at: 2968 3027 3514 3589 4042 2968 Size: 59 487 75 453 6013 Fragments arranged by size: 6013 487 453 75 59 SacI G_AGCT'C Cuts at: 2697 2721 3600 4080 2697 Size: 24 879 480 5704 Fragments arranged by size: 5704 879 480 24 SacII CC_GC'GG Cuts at: 3631 3631 Size: 7087 SalI G'TCGA_C Cuts at: 1936 4429 1936 Size: 2493 4594 SapI GCTCTTCn'nnn_ Cuts at: 2124 2702 3110 2124 Size: 578 408 6101 Fragments arranged by size: 6101 578 408 SbfI CC_TGCA'GG Cuts at: 4251 4439 4251 Size: 188 6899 ScaI AGT'ACT Cuts at: 2423 2993 5335 7047 2423 Size: 570 2342 1712 2463 Fragments arranged by size: 2463 2342 1712 570 SexAI A'CCwGG_T Cuts at: 3057 3057 Size: 7087 SfcI C'TryA_G Cuts at: 826 1045 2753 2939 3302 3332 3515 3584 3590 Size: 219 1708 186 363 30 183 69 6 Cuts at: 3590 3677 3824 3836 3866 4247 4366 4435 5570 Size: 87 147 12 30 381 119 69 1135 Cuts at: 5570 6251 6442 826 Size: 681 191 1471 Fragments arranged by size: 1708 1471 1135 681 381 363 219 191 186 183 147 119 87 69 69 30 30 12 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2054 3623 2054 Size: 1569 5518 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7087 SmaI CCC'GGG Cuts at: 1872 2876 4414 1872 Size: 1004 1538 4545 Fragments arranged by size: 4545 1538 1004 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2435 3536 3761 4079 5191 Size: 197 926 384 108 1101 225 318 1112 Cuts at: 5191 6092 6354 820 Size: 901 262 1553 Fragments arranged by size: 1553 1112 1101 926 901 384 318 262 225 197 108 SnaBI TAC'GTA Cuts at: 493 4878 493 Size: 4385 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7087 SphI G_CATG'C Cuts at: 1132 4445 1132 Size: 3313 3774 SspI AAT'ATT Cuts at: 5 52 5011 5 Size: 47 4959 2081 Fragments arranged by size: 4959 2081 47 StuI AGG'CCT Cuts at: 1517 2648 1517 Size: 1131 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 4314 4526 513 Size: 552 939 2310 212 3074 Fragments arranged by size: 3074 2310 939 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 3404 5114 5131 5284 5469 5914 Size: 1255 1215 609 1710 17 153 185 445 Cuts at: 5914 325 Size: 1498 Fragments arranged by size: 1710 1498 1255 1215 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 2745 2991 Size: 274 80 33 51 1443 444 324 246 Cuts at: 2991 3066 4065 5333 7045 96 Size: 75 999 1268 1712 138 Fragments arranged by size: 1712 1443 1268 999 444 324 274 246 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 2774 2806 7047 7079 2246 Size: 32 496 32 4241 32 2254 Fragments arranged by size: 4241 2254 496 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7087 XbaI T'CTAG_A Cuts at: 4423 4423 Size: 7087 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7087 XmaI C'CCGG_G Cuts at: 1870 2874 4412 1870 Size: 1004 1538 4545 Fragments arranged by size: 4545 1538 1004 XmnI GAAnn'nnTTC Cuts at: 5216 5216 Size: 7087 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4895 276 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SanDI SfoI SrfI SwaI XcmI