(Linear) (Six-base) MAPSORT of: KIEE4225.seq Check: 9872 from: 1 to: 2382 KIEE4225.orf With 182 enzymes: SgfI * July 3, 2012 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 67 927 2382 Size: 67 860 1455 Fragments arranged by size: 1455 860 67 AccI GT'mk_AC Cuts at: 0 566 2382 Size: 566 1816 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1543 2116 2137 2382 Size: 1543 573 21 245 Fragments arranged by size: 1543 573 245 21 AflIII A'CryG_T Cuts at: 0 254 2382 Size: 254 2128 AhdI GACnn_n'nnGTC Cuts at: 0 156 1368 2382 Size: 156 1212 1014 Fragments arranged by size: 1212 1014 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2006 2382 Size: 2006 376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 181 213 403 435 481 513 895 927 Size: 181 32 190 32 46 32 382 32 Cuts at: 927 2382 Size: 1455 Fragments arranged by size: 1455 382 190 181 46 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 381 1716 1839 2373 2382 Size: 381 1335 123 534 9 Fragments arranged by size: 1335 534 381 123 9 ApaI G_GGCC'C Cuts at: 0 1431 2125 2382 Size: 1431 694 257 Fragments arranged by size: 1431 694 257 AvaI C'yCGr_G Cuts at: 0 715 869 2382 Size: 715 154 1513 Fragments arranged by size: 1513 715 154 BamHI G'GATC_C Cuts at: 0 260 2382 Size: 260 2122 BanII G_rGCy'C Cuts at: 0 10 22 692 716 722 1431 1595 2075 Size: 10 12 670 24 6 709 164 480 Cuts at: 2075 2125 2382 Size: 50 257 Fragments arranged by size: 709 670 480 257 164 50 24 12 10 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1367 2382 Size: 1367 1015 BbvCI CC'TCA_GC Cuts at: 0 88 2382 Size: 88 2294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 453 487 2382 Size: 453 34 1895 Fragments arranged by size: 1895 453 34 BglI GCCn_nnn'nGGC Cuts at: 0 49 1618 2382 Size: 49 1569 764 Fragments arranged by size: 1569 764 49 BlpI GC'TnA_GC Cuts at: 0 1873 2161 2382 Size: 1873 288 221 Fragments arranged by size: 1873 288 221 Bme1580I G_kGCm'C Cuts at: 0 1431 2125 2382 Size: 1431 694 257 Fragments arranged by size: 1431 694 257 BmrI ACTGGGnnnn_n' Cuts at: 0 978 1432 2382 Size: 978 454 950 Fragments arranged by size: 978 950 454 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 29 168 940 2343 2382 Size: 29 139 772 1403 39 Fragments arranged by size: 1403 772 139 39 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 163 265 922 1108 1399 1411 2020 Size: 124 39 102 657 186 291 12 609 Cuts at: 2020 2317 2382 Size: 297 65 Fragments arranged by size: 657 609 297 291 186 124 102 65 39 12 Bpu10I CC'TnA_GC Cuts at: 0 88 235 2382 Size: 88 147 2147 Fragments arranged by size: 2147 147 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 415 1516 1777 2059 2382 Size: 307 108 1101 261 282 323 Fragments arranged by size: 1101 323 307 282 261 108 BsaI GGTCTCn'nnnn_ Cuts at: 0 1375 2382 Size: 1375 1007 BsaAI yAC'GTr Cuts at: 0 257 2382 Size: 257 2125 BsaBI GATnn'nnATC Cuts at: 0 1138 2382 Size: 1138 1244 BsaHI Gr'CG_yC Cuts at: 0 2339 2382 Size: 2339 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 31 61 181 211 241 271 403 433 Size: 31 30 120 30 30 30 132 30 Cuts at: 433 895 925 964 994 1081 1111 2382 Size: 462 30 39 30 87 30 1271 Fragments arranged by size: 1271 462 132 120 87 39 31 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 189 207 216 262 307 607 649 Size: 18 171 18 9 46 45 300 42 Cuts at: 649 652 1141 1243 1531 1936 2104 2224 2382 Size: 3 489 102 288 405 168 120 158 Fragments arranged by size: 489 405 300 288 171 168 158 120 102 46 45 42 18 18 9 3 BseYI C'CCAG_C Cuts at: 0 700 2382 Size: 700 1682 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1296 1829 2382 Size: 405 891 533 553 Fragments arranged by size: 891 553 533 405 BsiEI CG_ry'CG Cuts at: 0 1289 1632 2305 2382 Size: 1289 343 673 77 Fragments arranged by size: 1289 673 343 77 BsiHKAI G_wGCw'C Cuts at: 0 692 716 1315 1595 2075 2382 Size: 692 24 599 280 480 307 Fragments arranged by size: 692 599 480 307 280 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1405 1753 2382 Size: 1405 348 629 Fragments arranged by size: 1405 629 348 Bsp1286I G_dGCh'C Cuts at: 0 10 22 692 716 722 1315 1431 1595 Size: 10 12 670 24 6 593 116 164 Cuts at: 1595 2075 2125 2382 Size: 480 50 257 Fragments arranged by size: 670 593 480 257 164 116 50 24 12 10 6 BspHI T'CATG_A Cuts at: 0 545 2382 Size: 545 1837 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 67 927 1304 2382 Size: 67 860 377 1078 Fragments arranged by size: 1078 860 377 67 BsrBI CCG'CTC Cuts at: 0 2301 2382 Size: 2301 81 BsrDI GCAATG_nn' Cuts at: 0 1988 2382 Size: 1988 394 BsrFI r'CCGG_y Cuts at: 0 134 677 1163 2304 2382 Size: 134 543 486 1141 78 Fragments arranged by size: 1141 543 486 134 78 BssHII G'CGCG_C Cuts at: 0 1809 2278 2382 Size: 1809 469 104 Fragments arranged by size: 1809 469 104 BssSI C'ACGA_G Cuts at: 0 493 2382 Size: 493 1889 BstYI r'GATC_y Cuts at: 0 260 2003 2382 Size: 260 1743 379 Fragments arranged by size: 1743 379 260 Bsu36I CC'TnA_GG Cuts at: 0 632 2382 Size: 632 1750 BtgI C'CryG_G Cuts at: 0 392 1623 1648 2382 Size: 392 1231 25 734 Fragments arranged by size: 1231 734 392 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 456 2039 2288 2382 Size: 456 1583 249 94 Fragments arranged by size: 1583 456 249 94 BtsI GCAGTG_nn' Cuts at: 0 1279 2382 Size: 1279 1103 DrdI GACnn_nn'nnGTC Cuts at: 0 403 1036 2382 Size: 403 633 1346 Fragments arranged by size: 1346 633 403 EaeI y'GGCC_r Cuts at: 0 113 707 1286 1629 1718 1970 2302 2306 Size: 113 594 579 343 89 252 332 4 Cuts at: 2306 2351 2382 Size: 45 31 Fragments arranged by size: 594 579 343 332 252 113 89 45 31 4 EagI C'GGCC_G Cuts at: 0 1286 1629 2302 2382 Size: 1286 343 673 80 Fragments arranged by size: 1286 673 343 80 EarI CTCTTCn'nnn_ Cuts at: 0 119 541 697 1075 1105 2382 Size: 119 422 156 378 30 1277 Fragments arranged by size: 1277 422 378 156 119 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 201 964 1240 1819 1966 2278 2382 Size: 201 763 276 579 147 312 104 Fragments arranged by size: 763 579 312 276 201 147 104 EcoICRI GAG'CTC Cuts at: 0 690 714 1593 2073 2382 Size: 690 24 879 480 309 Fragments arranged by size: 879 690 480 309 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 163 265 922 1108 1399 1411 1543 Size: 124 39 102 657 186 291 12 132 Cuts at: 1543 2020 2116 2137 2317 2382 Size: 477 96 21 180 65 Fragments arranged by size: 657 477 291 186 180 132 124 102 96 65 39 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 247 523 934 1195 1300 1720 2382 Size: 247 276 411 261 105 420 662 Fragments arranged by size: 662 420 411 276 261 247 105 EcoO109I rG'GnC_Cy Cuts at: 0 42 531 1293 1427 1428 1713 2121 2139 Size: 42 489 762 134 1 285 408 18 Cuts at: 2139 2382 Size: 243 Fragments arranged by size: 762 489 408 285 243 134 42 18 1 FseI GG_CCGG'CC Cuts at: 0 2308 2382 Size: 2308 74 HaeII r_GCGC'y Cuts at: 0 392 599 1531 1819 2237 2382 Size: 392 207 932 288 418 145 Fragments arranged by size: 932 418 392 288 207 145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 31 48 63 142 174 964 996 Size: 16 15 17 15 79 32 790 32 Cuts at: 996 1354 1386 1680 1712 2173 2205 2382 Size: 358 32 294 32 461 32 177 Fragments arranged by size: 790 461 358 294 177 79 32 32 32 32 32 17 16 15 15 HindIII A'AGCT_T Cuts at: 0 792 2382 Size: 792 1590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 254 2183 2382 Size: 254 1929 199 Fragments arranged by size: 1929 254 199 MscI TGG'CCA Cuts at: 0 115 2382 Size: 115 2267 MslI CAynn'nnrTG Cuts at: 0 397 625 805 886 2382 Size: 397 228 180 81 1496 Fragments arranged by size: 1496 397 228 180 81 MspA1I CmG'CkG Cuts at: 0 963 1022 1179 1509 1584 1625 1634 2037 Size: 963 59 157 330 75 41 9 403 Cuts at: 2037 2145 2382 Size: 108 237 Fragments arranged by size: 963 403 330 237 157 108 75 59 41 9 NaeI GCC'GGC Cuts at: 0 2306 2382 Size: 2306 76 NgoMIV G'CCGG_C Cuts at: 0 2304 2382 Size: 2304 78 NotI GC'GGCC_GC Cuts at: 0 1629 2382 Size: 1629 753 NspI r_CATG'y Cuts at: 0 402 747 2382 Size: 402 345 1635 Fragments arranged by size: 1635 402 345 PflMI CCAn_nnn'nTGG Cuts at: 0 287 371 1554 1716 2373 2382 Size: 287 84 1183 162 657 9 Fragments arranged by size: 1183 657 287 162 84 9 PfoI T'CCnGG_A Cuts at: 0 140 2382 Size: 140 2242 PmlI CAC'GTG Cuts at: 0 257 2382 Size: 257 2125 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 181 213 403 435 895 927 2382 Size: 181 32 190 32 460 32 1455 Fragments arranged by size: 1455 460 190 181 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 531 1293 2139 2382 Size: 531 762 846 243 Fragments arranged by size: 846 762 531 243 PspOMI G'GGCC_C Cuts at: 0 1427 2121 2382 Size: 1427 694 261 Fragments arranged by size: 1427 694 261 PstI C_TGCA'G Cuts at: 0 752 938 1301 1514 1583 1589 1676 1823 Size: 752 186 363 213 69 6 87 147 Cuts at: 1823 1835 1865 2246 2382 Size: 12 30 381 136 Fragments arranged by size: 752 381 363 213 186 147 136 87 69 30 12 6 PvuII CAG'CTG Cuts at: 0 963 1022 1509 1584 2037 2382 Size: 963 59 487 75 453 345 Fragments arranged by size: 963 487 453 345 75 59 SacI G_AGCT'C Cuts at: 0 692 716 1595 2075 2382 Size: 692 24 879 480 307 Fragments arranged by size: 879 692 480 307 24 SacII CC_GC'GG Cuts at: 0 1626 2382 Size: 1626 756 SapI GCTCTTCn'nnn_ Cuts at: 0 119 697 1105 2382 Size: 119 578 408 1277 Fragments arranged by size: 1277 578 408 119 SbfI CC_TGCA'GG Cuts at: 0 2246 2382 Size: 2246 136 ScaI AGT'ACT Cuts at: 0 418 988 2382 Size: 418 570 1394 Fragments arranged by size: 1394 570 418 SexAI A'CCwGG_T Cuts at: 0 1052 2382 Size: 1052 1330 SfcI C'TryA_G Cuts at: 0 748 934 1297 1327 1510 1579 1585 1672 Size: 748 186 363 30 183 69 6 87 Cuts at: 1672 1819 1831 1861 2242 2361 2382 Size: 147 12 30 381 119 21 Fragments arranged by size: 748 381 363 186 183 147 119 87 69 30 30 21 12 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 49 1618 2382 Size: 49 1569 764 Fragments arranged by size: 1569 764 49 SmaI CCC'GGG Cuts at: 0 871 2382 Size: 871 1511 SmlI C'TyrA_G Cuts at: 0 322 430 1531 1756 2074 2382 Size: 322 108 1101 225 318 308 Fragments arranged by size: 1101 322 318 308 225 108 StuI AGG'CCT Cuts at: 0 643 2382 Size: 643 1739 StyI C'CwwG_G Cuts at: 0 2309 2382 Size: 2309 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 790 1399 2382 Size: 790 609 983 Fragments arranged by size: 983 790 609 TatI w'GTAC_w Cuts at: 0 416 740 986 1061 2060 2382 Size: 416 324 246 75 999 322 Fragments arranged by size: 999 416 324 322 246 75 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 241 273 769 801 2382 Size: 241 32 496 32 1581 Fragments arranged by size: 1581 496 241 32 32 XmaI C'CCGG_G Cuts at: 0 869 2382 Size: 869 1513 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI ApaI AvaI BamHI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BciVI BclI BfrBI BglII BmgBI BmtI BsaWI BsiWI BsmI BspEI BsrGI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV FalI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NruI NsiI PacI PasI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI