################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccII AclI AflII AflIII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BclI BcnI BfrI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI BspHI BspLU11I BsrBI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CpoI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FauI FbaI FseI FspI HgaI HpaI KpnI Ksp632I KspI MaeII MamI MluI MvnI NciI NcoI NheI NotI NruI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 712 GTAGAC GT^[AC][GT]AC AccIII 329 TCCGGA T^CCGGA AcyI 68 GGCGCC G[GA]^CG[CT]C AhdI 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 259 GTGCAC G^TGCAC AocI 2237 CCTCAGG CC^T[ACGT]AGG ApaLI 259 GTGCAC G^TGCAC AspEI 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1197 TGGCCA TGG^CCA BbeI 68 GGCGCC GGCGC^C BciVI 684 GGATAC GTATCC(6/5) BfaI 1000 CTAG C^TAG BglI 802 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 521 GCTCAGC GC^T[ACGT]AGC Bpu1102I 521 GCTCAGC GC^T[ACGT]AGC BsaHI 68 GGCGCC G[GA]^CG[CT]C BsaI 641 GAGACC GGTCTC(1/5) BseAI 329 TCCGGA T^CCGGA Bsp1407I 2121 TGTACA T^GTACA BspDI 1148 ATCGAT AT^CGAT BspEI 329 TCCGGA T^CCGGA BspMI 811 GCAGGT ACCTGC(4/8) BsrFI 218 GCCGGC [GA]^CCGG[CT] BsrGI 2121 TGTACA T^GTACA BssSI 187 CACGAG CACGAG(-5/-1) BstBI 1514 TTCGAA TT^CGAA BstXI 2027 CCAGCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 275 AGATCC [GA]^GATC[CT] Bsu36I 2237 CCTCAGG CC^T[ACGT]AGG CelII 521 GCTCAGC GC^T[ACGT]AGC CfoI 69 GCGC GCG^C Cfr10I 218 GCCGGC [GA]^CCGG[CT] ClaI 1148 ATCGAT AT^CGAT EaeI 1197 TGGCCA [CT]^GGCC[GA] Eam1105I 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2237 CCTCAGG CC^T[ACGT]AGG EcoT22I 451 ATGCAT ATGCA^T HaeII 68 GGCGCC [GA]GCGC^[CT] HhaI 69 GCGC GCG^C Hin1I 68 GGCGCC G[GA]^CG[CT]C HinP1I 69 GCGC G^CGC HincII 840 GTTGAC GT[CT]^[GA]AC HindII 840 GTTGAC GT[CT]^[GA]AC KasI 68 GGCGCC G^GCGCC MaeI 1000 CTAG C^TAG MfeI 175 CAATTG C^AATTG MflI 275 AGATCC [GA]^GATC[CT] MluNI 1197 TGGCCA TGG^CCA MroI 329 TCCGGA T^CCGGA MscI 1197 TGGCCA TGG^CCA MunI 175 CAATTG C^AATTG NaeI 218 GCCGGC GCC^GGC NarI 68 GGCGCC GG^CGCC NdeI 695 CATATG CA^TATG NgoMIV 218 GCCGGC G^CCGGC NsiI 451 ATGCAT ATGCA^T NspV 1514 TTCGAA TT^CGAA PflMI 792 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 343 CAGCTG CAG^CTG SfoI 68 GGCGCC GGC^GCC SfuI 1514 TTCGAA TT^CGAA SspBI 2121 TGTACA T^GTACA Tsp45I 2102 GTGAC ^GT[GC]AC Van91I 792 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 275 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 202 CCGC CCGC(-3/-1) 237 CCGC BanI 68 GGCGCC G^G[CT][GA]CC 1871 GGCACC BbsI 1251 GAAGAC GAAGAC(2/6) 1098 GTCTTC BpuAI 1251 GAAGAC GAAGAC(2/6) 1098 GTCTTC BsaWI 329 TCCGGA [AT]^CCGG[AT] 2140 ACCGGA BsgI 1546 CTGCAC GTGCAG(16/14) 340 GTGCAG DraI 862 TTTAAA TTT^AAA 1128 TTTAAA EcoRI 1796 GAATTC G^AATTC 1964 GAATTC EcoT14I 266 CCAAGG C^C[AT][AT]GG 806 CCTTGG HindIII 1169 AAGCTT A^AGCTT 2093 AAGCTT MaeIII 182 GTTAC ^GT[ACGT]AC 2102 GTGAC MspA1I 237 CCGCTG C[AC]G^C[GT]G 343 CAGCTG PpuMI 701 AGGACCT [GA]G^G[AT]CC[CT] 1541 AGGTCCT PstI 8 CTGCAG CTGCA^G 763 CTGCAG SacI 229 GAGCTC GAGCT^C 325 GAGCTC SmlI 295 CTTGAG C^T[CT][GA]AG 623 CTCAAG StuI 27 AGGCCT AGG^CCT 1337 AGGCCT StyI 266 CCAAGG C^C[AT][AT]GG 806 CCTTGG XcmI 186 CCACGAGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 570 CCACCTCCCCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI