(Circular) (Six-base) MAPSORT of: pFN21AB6247.seq Check: 9989 from: 1 to: 6967 pFN21AB6247 6967 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2807 2807 Size: 6967 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4777 278 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 4310 1937 Size: 781 1592 4594 Fragments arranged by size: 4594 1592 781 Acc65I G'GTAC_C Cuts at: 4288 4288 Size: 6967 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 3247 3667 5030 6414 6682 1632 Size: 526 1089 420 1363 1384 268 1917 Fragments arranged by size: 1917 1384 1363 1089 526 420 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6770 AflIII A'CryG_T Cuts at: 1203 5866 6843 1203 Size: 4663 977 1327 Fragments arranged by size: 4663 1327 977 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 3545 5696 3545 Size: 2151 4816 AleI CACnn'nnGTG Cuts at: 1166 2412 3894 3978 1166 Size: 1246 1482 84 4155 Fragments arranged by size: 4155 1482 1246 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6967 AlwNI CAG_nnn'CTG Cuts at: 2284 2311 2534 2936 3550 4265 4710 6282 2284 Size: 27 223 402 614 715 445 1572 2969 Fragments arranged by size: 2969 1572 715 614 445 402 223 27 ApaLI G'TGCA_C Cuts at: 2264 5024 6180 2264 Size: 2760 1156 3051 Fragments arranged by size: 3051 2760 1156 ApoI r'AATT_y Cuts at: 1493 2026 2861 3801 3969 4276 4502 4654 6763 Size: 533 835 940 168 307 226 152 2109 Cuts at: 6763 1493 Size: 1697 Fragments arranged by size: 2109 1697 940 835 533 307 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 4292 1870 Size: 73 2349 4545 Fragments arranged by size: 4545 2349 73 BamHI G'GATC_C Cuts at: 4297 4664 4297 Size: 367 6600 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 3876 4288 5743 618 Size: 325 217 627 286 1803 412 1455 1842 Fragments arranged by size: 1842 1803 1455 627 412 325 286 217 BanII G_rGCy'C Cuts at: 1391 2238 2334 2528 2542 2767 4286 1391 Size: 847 96 194 14 225 1519 4072 Fragments arranged by size: 4072 1519 847 225 194 96 14 BbeI G_GCGC'C Cuts at: 2077 2077 Size: 6967 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3096 3263 928 Size: 971 1197 167 4632 Fragments arranged by size: 4632 1197 971 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5158 5192 1903 Size: 34 3221 34 3678 Fragments arranged by size: 3678 3221 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2683 4859 6069 2683 Size: 2176 1210 3581 Fragments arranged by size: 3581 2176 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6967 BfrBI ATG'CAT Cuts at: 2458 2458 Size: 6967 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 5578 136 Size: 107 122 71 631 605 1141 2765 1525 Fragments arranged by size: 2765 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 6962 6962 Size: 6967 BlpI GC'TnA_GC Cuts at: 2527 4384 2527 Size: 1857 5110 Bme1580I G_kGCm'C Cuts at: 1790 2268 5028 6184 1790 Size: 478 2760 1156 2573 Fragments arranged by size: 2760 2573 1156 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2577 3483 3651 Size: 416 53 83 399 24 1160 906 168 Cuts at: 3651 3735 4071 4698 5656 442 Size: 84 336 627 958 1753 Fragments arranged by size: 1753 1160 958 906 627 416 399 336 168 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6967 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4258 4258 Size: 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2306 2556 2567 2595 2958 3243 3831 Size: 875 192 250 11 28 363 285 588 Cuts at: 3831 4015 4251 5627 1239 Size: 184 236 1376 2579 Fragments arranged by size: 2579 1376 875 588 363 285 250 236 192 184 28 11 Bpu10I CC'TnA_GC Cuts at: 2312 2312 Size: 6967 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 2613 5092 5957 6255 2321 Size: 292 2479 865 298 3033 Fragments arranged by size: 3033 2479 865 298 292 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2640 5630 882 Size: 617 1141 2990 2219 Fragments arranged by size: 2990 2219 1141 617 BsaAI yAC'GTr Cuts at: 493 1204 4758 493 Size: 711 3554 2702 Fragments arranged by size: 3554 2702 711 BsaBI GATnn'nnATC Cuts at: 6961 6961 Size: 6967 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 4774 5156 5791 Size: 53 83 186 936 541 2700 382 635 Cuts at: 5791 275 Size: 1451 Fragments arranged by size: 2700 1451 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4145 5400 5798 6072 6219 6630 2334 Size: 1811 1255 398 274 147 411 2671 Fragments arranged by size: 2671 1811 1255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2562 2592 3682 3712 1178 Size: 30 165 30 1159 30 1090 30 4433 Fragments arranged by size: 4433 1159 1090 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2438 2480 2490 1186 Size: 1201 51 42 10 5663 Fragments arranged by size: 5663 1201 51 42 10 BseYI C'CCAG_C Cuts at: 2437 3276 6170 6666 2437 Size: 839 2894 496 2738 Fragments arranged by size: 2894 2738 839 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 3536 1163 Size: 573 630 1170 4594 Fragments arranged by size: 4594 1170 630 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2051 2238 2268 2334 5028 5113 6184 2051 Size: 187 30 66 2694 85 1071 2834 Fragments arranged by size: 2834 2694 1071 187 85 66 30 BsmI GAATG_Cn' Cuts at: 3329 3497 3512 3596 4478 4571 3329 Size: 168 15 84 882 93 5725 Fragments arranged by size: 5725 882 168 93 84 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2238 2268 2334 2528 2542 2767 Size: 399 261 187 30 66 194 14 225 Cuts at: 2767 4286 5028 5113 6184 1391 Size: 1519 742 85 1071 2174 Fragments arranged by size: 2174 1519 1071 742 399 261 225 194 187 85 66 30 14 BspEI T'CCGG_A Cuts at: 2334 6630 2334 Size: 4296 2671 BspHI T'CATG_A Cuts at: 4856 6586 4856 Size: 1730 5237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 4322 844 Size: 1963 1515 3489 Fragments arranged by size: 3489 1963 1515 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3502 3586 3922 4090 5462 5636 65 Size: 1183 2254 84 336 168 1372 174 1396 Fragments arranged by size: 2254 1396 1372 1183 336 174 168 84 BsrFI r'CCGG_y Cuts at: 1700 1810 2223 5611 5798 1700 Size: 110 413 3388 187 2869 Fragments arranged by size: 3388 2869 413 187 110 BsrGI T'GTAC_A Cuts at: 96 4126 96 Size: 4030 2937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 2192 5027 6039 2192 Size: 2835 1012 3120 Fragments arranged by size: 3120 2835 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6967 BstBI TT'CG_AA Cuts at: 3520 5771 3520 Size: 2251 4716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstXI CCAn_nnnn'nTGG Cuts at: 4039 4039 Size: 6967 BstYI r'GATC_y Cuts at: 1972 2280 4297 4664 5049 5066 6518 6604 6616 Size: 308 2017 367 385 17 1452 86 12 Cuts at: 6616 6962 1972 Size: 346 1977 Fragments arranged by size: 2017 1977 1452 385 367 346 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4243 4243 Size: 6967 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6415 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6228 Fragments arranged by size: 6228 683 56 BtsI GCAGTG_nn' Cuts at: 2010 2049 2777 3334 4483 5277 5297 2010 Size: 39 728 557 1149 794 20 3680 Fragments arranged by size: 3680 1149 794 728 557 39 20 ClaI AT'CG_AT Cuts at: 1554 3154 1554 Size: 1600 5367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3484 3519 3568 3603 3904 3939 4072 Size: 35 2920 35 49 35 301 35 133 Cuts at: 4072 4107 529 Size: 35 3389 Fragments arranged by size: 3389 2920 301 133 49 35 35 35 35 35 DraI TTT'AAA Cuts at: 2869 3135 4271 2869 Size: 266 1136 5565 Fragments arranged by size: 5565 1136 266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5974 809 Size: 507 4658 1802 Fragments arranged by size: 4658 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4414 5303 Size: 54 1425 50 275 18 1372 1212 889 Cuts at: 5303 6932 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1372 1212 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4897 1353 Size: 3544 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5554 5928 6074 387 Size: 1064 138 3965 374 146 1280 Fragments arranged by size: 3965 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2236 2332 2236 Size: 96 6871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2158 2306 2556 2567 2595 2958 Size: 393 482 44 148 250 11 28 363 Cuts at: 2958 3243 3247 3667 3831 4015 4251 5030 5627 Size: 285 4 420 164 184 236 779 597 Cuts at: 5627 6414 6682 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 779 597 482 420 393 363 285 268 250 236 184 164 148 44 28 11 4 EcoO109I rG'GnC_Cy Cuts at: 2682 2707 3547 2682 Size: 25 840 6102 Fragments arranged by size: 6102 840 25 EcoRI G'AATT_C Cuts at: 3801 3969 4276 4654 3801 Size: 168 307 378 6114 Fragments arranged by size: 6114 378 307 168 FspI TGC'GCA Cuts at: 5473 5473 Size: 6967 HaeII r_GCGC'y Cuts at: 1678 2077 6114 1678 Size: 399 4037 2531 Fragments arranged by size: 4037 2531 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 2696 2728 3531 Size: 32 135 32 889 32 203 32 803 Cuts at: 3531 3563 3682 3714 3850 3882 5608 5640 5682 Size: 32 119 32 136 32 1726 32 42 Cuts at: 5682 5714 1373 Size: 32 2626 Fragments arranged by size: 2626 1726 889 803 203 136 135 119 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2847 4311 4557 669 Size: 988 281 909 1464 246 3079 Fragments arranged by size: 3079 1464 988 909 281 246 HindIII A'AGCT_T Cuts at: 748 3174 4098 748 Size: 2426 924 3617 Fragments arranged by size: 3617 2426 924 HpaI GTT'AAC Cuts at: 4557 4557 Size: 6967 KasI G'GCGC_C Cuts at: 2073 2073 Size: 6967 KpnI G_GTAC'C Cuts at: 4292 4292 Size: 6967 MfeI C'AATT_G Cuts at: 2180 4566 2180 Size: 2386 4581 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3668 6081 6265 1305 Size: 2363 2413 184 2007 Fragments arranged by size: 2413 2363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3204 10 Size: 54 1425 343 1372 3773 Fragments arranged by size: 3773 1425 1372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2412 3742 3894 3978 4925 5284 5443 Size: 648 1246 1330 152 84 947 359 159 Cuts at: 5443 518 Size: 2042 Fragments arranged by size: 2042 1330 1246 947 648 359 159 152 84 MspA1I CmG'CkG Cuts at: 1606 2244 2350 4383 5060 6208 6453 1606 Size: 638 106 2033 677 1148 245 2120 Fragments arranged by size: 2120 2033 1148 677 638 245 106 NaeI GCC'GGC Cuts at: 2225 2225 Size: 6967 NarI GG'CG_CC Cuts at: 2074 2074 Size: 6967 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6415 NdeI CA'TA_TG Cuts at: 387 2701 387 Size: 2314 4653 NgoMIV G'CCGG_C Cuts at: 2223 2223 Size: 6967 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6967 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NsiI A_TGCA'T Cuts at: 2460 2460 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 4325 5870 1132 Size: 97 3096 1545 2229 Fragments arranged by size: 3096 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2324 2564 1348 Size: 976 240 5751 Fragments arranged by size: 5751 976 240 PciI A'CATG_T Cuts at: 5866 5866 Size: 6967 PflMI CCAn_nnn'nTGG Cuts at: 1108 2803 1108 Size: 1695 5272 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 6967 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 3547 2707 Size: 840 6127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6967 PsiI TTA'TAA Cuts at: 4537 4537 Size: 6967 PstI C_TGCA'G Cuts at: 830 2017 2772 4319 830 Size: 1187 755 1547 3478 Fragments arranged by size: 3478 1547 1187 755 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 PvuII CAG'CTG Cuts at: 2350 2350 Size: 6967 SacI G_AGCT'C Cuts at: 2238 2334 2238 Size: 96 6871 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 5215 6927 5215 Size: 1712 5255 SfcI C'TryA_G Cuts at: 826 1045 2013 2715 2768 3346 4315 5450 6131 Size: 219 968 702 53 578 969 1135 681 Cuts at: 6131 6322 826 Size: 191 1471 Fragments arranged by size: 1471 1135 969 968 702 681 578 219 191 53 SfoI GGC'GCC Cuts at: 2075 2075 Size: 6967 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 4294 1872 Size: 2422 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 2628 5071 5972 6234 820 Size: 197 926 357 328 2443 901 262 1553 Fragments arranged by size: 2443 1553 926 901 357 328 262 197 SnaBI TAC'GTA Cuts at: 493 4758 493 Size: 4265 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6967 SphI G_CATG'C Cuts at: 1132 4325 1132 Size: 3193 3774 SspI AAT'ATT Cuts at: 5 52 4891 5 Size: 47 4839 2081 Fragments arranged by size: 4839 2081 47 StuI AGG'CCT Cuts at: 1517 2034 3344 1517 Size: 517 1310 5140 Fragments arranged by size: 5140 1310 517 StyI C'CwwG_G Cuts at: 513 1065 2271 2811 4406 513 Size: 552 1206 540 1595 3074 Fragments arranged by size: 3074 1595 1206 552 540 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2332 4994 5011 5164 5349 5794 325 Size: 1255 752 2662 17 153 185 445 1498 Fragments arranged by size: 2662 1498 1255 752 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2603 2665 4126 Size: 274 80 33 51 1443 626 62 1461 Cuts at: 4126 5213 6925 96 Size: 1087 1712 138 Fragments arranged by size: 1712 1461 1443 1087 626 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2562 2594 6927 6959 2562 Size: 32 4333 32 2570 Fragments arranged by size: 4333 2570 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 4303 4303 Size: 6967 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2198 2582 2198 Size: 384 6583 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 4292 1870 Size: 2422 4545 XmnI GAAnn'nnTTC Cuts at: 3260 3764 4016 5096 3260 Size: 504 252 1080 5131 Fragments arranged by size: 5131 1080 504 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4775 276 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI