(Linear) (Six-base) MAPSORT of: KIBB6247.seq Check: 2285 from: 1 to: 2262 KIBB6247.orf With 182 enzymes: SgfI * July 3, 2012 16:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 802 2262 Size: 802 1460 AccI GT'mk_AC Cuts at: 0 713 2262 Size: 713 1549 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 1242 1662 2262 Size: 153 1089 420 600 Fragments arranged by size: 1089 600 420 153 AhdI GACnn_n'nnGTC Cuts at: 0 1540 2262 Size: 1540 722 AleI CACnn'nnGTG Cuts at: 0 407 1889 1973 2262 Size: 407 1482 84 289 Fragments arranged by size: 1482 407 289 84 AlwNI CAG_nnn'CTG Cuts at: 0 279 306 529 931 1545 2262 Size: 279 27 223 402 614 717 Fragments arranged by size: 717 614 402 279 223 27 ApaLI G'TGCA_C Cuts at: 0 259 2262 Size: 259 2003 ApoI r'AATT_y Cuts at: 0 21 856 1796 1964 2262 Size: 21 835 940 168 298 Fragments arranged by size: 940 835 298 168 21 BanI G'GyrC_C Cuts at: 0 68 1871 2262 Size: 68 1803 391 Fragments arranged by size: 1803 391 68 BanII G_rGCy'C Cuts at: 0 233 329 523 537 762 2262 Size: 233 96 194 14 225 1500 Fragments arranged by size: 1500 233 225 194 96 14 BbeI G_GCGC'C Cuts at: 0 72 2262 Size: 72 2190 BbsI GAAGACnn'nnnn_ Cuts at: 0 1091 1258 2262 Size: 1091 167 1004 Fragments arranged by size: 1091 1004 167 BciVI GTATCCnnnnn_n' Cuts at: 0 678 2262 Size: 678 1584 BfrBI ATG'CAT Cuts at: 0 453 2262 Size: 453 1809 BglI GCCn_nnn'nGGC Cuts at: 0 808 2262 Size: 808 1454 BlpI GC'TnA_GC Cuts at: 0 522 2262 Size: 522 1740 Bme1580I G_kGCm'C Cuts at: 0 263 2262 Size: 263 1999 BmrI ACTGGGnnnn_n' Cuts at: 0 572 1478 1646 1730 2066 2262 Size: 572 906 168 84 336 196 Fragments arranged by size: 906 572 336 196 168 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2253 2262 Size: 2253 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 301 551 562 590 953 1238 1826 Size: 109 192 250 11 28 363 285 588 Cuts at: 1826 2010 2246 2262 Size: 184 236 16 Fragments arranged by size: 588 363 285 250 236 192 184 109 28 16 11 Bpu10I CC'TnA_GC Cuts at: 0 307 2262 Size: 307 1955 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 608 2262 Size: 316 292 1654 Fragments arranged by size: 1654 316 292 BsaI GGTCTCn'nnnn_ Cuts at: 0 635 2262 Size: 635 1627 BsaHI Gr'CG_yC Cuts at: 0 69 2262 Size: 69 2193 BsaWI w'CCGG_w Cuts at: 0 329 2140 2262 Size: 329 1811 122 Fragments arranged by size: 1811 329 122 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 557 587 1677 1707 2262 Size: 557 30 1090 30 555 Fragments arranged by size: 1090 557 555 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 382 433 475 485 2262 Size: 382 51 42 10 1777 Fragments arranged by size: 1777 382 51 42 10 BseYI C'CCAG_C Cuts at: 0 432 1271 2262 Size: 432 839 991 Fragments arranged by size: 991 839 432 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 1531 2262 Size: 361 1170 731 Fragments arranged by size: 1170 731 361 BsiHKAI G_wGCw'C Cuts at: 0 46 233 263 329 2262 Size: 46 187 30 66 1933 Fragments arranged by size: 1933 187 66 46 30 BsmI GAATG_Cn' Cuts at: 0 1324 1492 1507 1591 2262 Size: 1324 168 15 84 671 Fragments arranged by size: 1324 671 168 84 15 Bsp1286I G_dGCh'C Cuts at: 0 46 233 263 329 523 537 762 2262 Size: 46 187 30 66 194 14 225 1500 Fragments arranged by size: 1500 225 194 187 66 46 30 14 BspEI T'CCGG_A Cuts at: 0 329 2262 Size: 329 1933 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 802 2262 Size: 802 1460 BsrDI GCAATG_nn' Cuts at: 0 1497 1581 1917 2085 2262 Size: 1497 84 336 168 177 Fragments arranged by size: 1497 336 177 168 84 BsrFI r'CCGG_y Cuts at: 0 218 2262 Size: 218 2044 BsrGI T'GTAC_A Cuts at: 0 2121 2262 Size: 2121 141 BssSI C'ACGA_G Cuts at: 0 187 2262 Size: 187 2075 BstBI TT'CG_AA Cuts at: 0 1515 2262 Size: 1515 747 BstXI CCAn_nnnn'nTGG Cuts at: 0 2034 2262 Size: 2034 228 BstYI r'GATC_y Cuts at: 0 275 2262 Size: 275 1987 Bsu36I CC'TnA_GG Cuts at: 0 2238 2262 Size: 2238 24 BtsI GCAGTG_nn' Cuts at: 0 5 44 772 1329 2262 Size: 5 39 728 557 933 Fragments arranged by size: 933 728 557 39 5 ClaI AT'CG_AT Cuts at: 0 1149 2262 Size: 1149 1113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1479 1514 1563 1598 1899 1934 2067 2102 Size: 1479 35 49 35 301 35 133 35 Cuts at: 2102 2262 Size: 160 Fragments arranged by size: 1479 301 160 133 49 35 35 35 35 DraI TTT'AAA Cuts at: 0 864 1130 2262 Size: 864 266 1132 Fragments arranged by size: 1132 864 266 EaeI y'GGCC_r Cuts at: 0 1197 2262 Size: 1197 1065 EcoICRI GAG'CTC Cuts at: 0 231 327 2262 Size: 231 96 1935 Fragments arranged by size: 1935 231 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 153 301 551 562 590 953 1238 Size: 109 44 148 250 11 28 363 285 Cuts at: 1238 1242 1662 1826 2010 2246 2262 Size: 4 420 164 184 236 16 Fragments arranged by size: 420 363 285 250 236 184 164 148 109 44 28 16 11 4 EcoO109I rG'GnC_Cy Cuts at: 0 677 702 1542 2262 Size: 677 25 840 720 Fragments arranged by size: 840 720 677 25 EcoRI G'AATT_C Cuts at: 0 1796 1964 2262 Size: 1796 168 298 Fragments arranged by size: 1796 298 168 HaeII r_GCGC'y Cuts at: 0 72 2262 Size: 72 2190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 456 488 691 723 1526 1558 1677 1709 Size: 456 32 203 32 803 32 119 32 Cuts at: 1709 1845 1877 2262 Size: 136 32 385 Fragments arranged by size: 803 456 385 203 136 119 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 842 2262 Size: 842 1420 HindIII A'AGCT_T Cuts at: 0 1169 2093 2262 Size: 1169 924 169 Fragments arranged by size: 1169 924 169 KasI G'GCGC_C Cuts at: 0 68 2262 Size: 68 2194 MfeI C'AATT_G Cuts at: 0 175 2262 Size: 175 2087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1663 2262 Size: 1663 599 MscI TGG'CCA Cuts at: 0 1199 2262 Size: 1199 1063 MslI CAynn'nnrTG Cuts at: 0 407 1737 1889 1973 2262 Size: 407 1330 152 84 289 Fragments arranged by size: 1330 407 289 152 84 MspA1I CmG'CkG Cuts at: 0 239 345 2262 Size: 239 106 1917 Fragments arranged by size: 1917 239 106 NaeI GCC'GGC Cuts at: 0 220 2262 Size: 220 2042 NarI GG'CG_CC Cuts at: 0 69 2262 Size: 69 2193 NdeI CA'TA_TG Cuts at: 0 696 2262 Size: 696 1566 NgoMIV G'CCGG_C Cuts at: 0 218 2262 Size: 218 2044 NsiI A_TGCA'T Cuts at: 0 455 2262 Size: 455 1807 PasI CC'CwG_GG Cuts at: 0 319 559 2262 Size: 319 240 1703 Fragments arranged by size: 1703 319 240 PflMI CCAn_nnn'nTGG Cuts at: 0 798 2262 Size: 798 1464 PfoI T'CCnGG_A Cuts at: 0 278 2262 Size: 278 1984 PpuMI rG'GwC_Cy Cuts at: 0 702 1542 2262 Size: 702 840 720 Fragments arranged by size: 840 720 702 PstI C_TGCA'G Cuts at: 0 12 767 2262 Size: 12 755 1495 Fragments arranged by size: 1495 755 12 PvuII CAG'CTG Cuts at: 0 345 2262 Size: 345 1917 SacI G_AGCT'C Cuts at: 0 233 329 2262 Size: 233 96 1933 Fragments arranged by size: 1933 233 96 SfcI C'TryA_G Cuts at: 0 8 710 763 1341 2262 Size: 8 702 53 578 921 Fragments arranged by size: 921 702 578 53 8 SfoI GGC'GCC Cuts at: 0 70 2262 Size: 70 2192 SmlI C'TyrA_G Cuts at: 0 295 623 2262 Size: 295 328 1639 Fragments arranged by size: 1639 328 295 StuI AGG'CCT Cuts at: 0 29 1339 2262 Size: 29 1310 923 Fragments arranged by size: 1310 923 29 StyI C'CwwG_G Cuts at: 0 266 806 2262 Size: 266 540 1456 Fragments arranged by size: 1456 540 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 327 2262 Size: 327 1935 TatI w'GTAC_w Cuts at: 0 598 660 2121 2262 Size: 598 62 1461 141 Fragments arranged by size: 1461 598 141 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 557 589 2262 Size: 557 32 1673 Fragments arranged by size: 1673 557 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 193 577 2262 Size: 193 384 1685 Fragments arranged by size: 1685 384 193 XmnI GAAnn'nnTTC Cuts at: 0 1255 1759 2011 2262 Size: 1255 504 252 251 Fragments arranged by size: 1255 504 252 251 Enzymes that do cut: AarI AccI AcuI AhdI AleI AlwNI ApaLI ApoI BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I BtsI ClaI CspCI DraI EaeI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI PasI PflMI PfoI PpuMI PstI PvuII SacI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BclI BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BssHII BstAPI BstEII BstZ17I BtgI BtgZI DraIII DrdI EagI EarI EciI EcoNI EcoRV FalI FseI FspI FspAI HpaI KpnI MluI NcoI NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI