################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 120 TCCGGA T^CCGGA AgeI 359 ACCGGT A^CCGGT AhdI 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 208 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 498 GGGCCC GGGCC^C AspEI 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 679 CTCGAG C^[CT]CG[GA]G BbeI 367 GGCGCC GGCGC^C BbrPI 199 CACGTG CAC^GTG BglI 538 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 120 TCCGGA T^CCGGA BsgI 514 CTGCAC GTGCAG(16/14) BsiEI 411 CGGCCG CG[GA][CT]^CG BsoBI 679 CTCGAG C^[CT]CG[GA]G BspEI 120 TCCGGA T^CCGGA BsrI 856 ACTGG ACTGG(1/-1) BstXI 548 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 275 AGATCC [GA]^GATC[CT] DsaI 50 CCGTGG C^C[GA][CT]GG EagI 411 CGGCCG C^GGCCG Eam1105I 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 124 GAAGAG CTCTTC(1/4) EclXI 411 CGGCCG C^GGCCG Eco52I 411 CGGCCG C^GGCCG HaeII 367 GGCGCC [GA]GCGC^[CT] HincII 460 GTCAAC GT[CT]^[GA]AC HindII 460 GTCAAC GT[CT]^[GA]AC KasI 367 GGCGCC G^GCGCC Ksp632I 124 GAAGAG CTCTTC(1/4) MaeIII 652 GTCAC ^GT[ACGT]AC MflI 275 AGATCC [GA]^GATC[CT] MroI 120 TCCGGA T^CCGGA MslI 855 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 367 GGCGCC GG^CGCC PaeR7I 679 CTCGAG C^TCGAG PinAI 359 ACCGGT A^CCGGT PmaCI 199 CACGTG CAC^GTG PmlI 199 CACGTG CAC^GTG PpuMI 317 GGGACCC [GA]G^G[AT]CC[CT] SfoI 367 GGCGCC GGC^GCC SmlI 679 CTCGAG C^T[CT][GA]AG Tsp45I 652 GTCAC ^GT[GC]AC XcmI 485 CCATCCCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 679 CTCGAG C^TCGAG XhoII 275 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 30 GTAC GT^AC 363 GTAC AlwI 276 GATCC GGATC(4/5) 443 GGATC AvaII 204 GGACC G^G[AT]CC 318 GGACC BanI 356 GGCACC G^G[CT][GA]CC 367 GGCGCC BanII 498 GGGCCC G[GA]GC[CT]^C 676 GGGCTC BpmI 894 CTCCAG CTGGAG(16/14) 778 CTGGAG BsaAI 199 CACGTG [CT]AC^GT[GA] 664 CACGTA BsaI 127 GAGACC GGTCTC(1/5) 341 GAGACC BsaWI 120 TCCGGA [AT]^CCGG[AT] 359 ACCGGT BsmAI 127 GAGAC GTCTC(1/5) 341 GAGAC DdeI 323 CTGAG C^T[ACGT]AG 656 CTGAG HgaI 10 GCGTC GACGC(5/10) 425 GCGTC HinfI 97 GAGTC G^A[ACGT]TC 509 GAGTC MboII 124 GAAGA GAAGA(8/7) 257 TCTTC MspA1I 730 CAGCTG C[AC]G^C[GT]G 792 CAGCTG PleI 97 GAGTC GAGTC(4/5) 509 GAGTC PvuII 730 CAGCTG CAG^CTG 792 CAGCTG RsaI 30 GTAC GT^AC 363 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AluI AspHI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MnlI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I