(Circular) (Six-base) MAPSORT of: pFN21AB6481.seq Check: 516 from: 1 to: 5638 pFN21AB6481 5638 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3701 5085 5353 1632 Size: 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 2364 4469 2364 Size: 2105 3533 AhdI GACnn_n'nnGTC Cuts at: 2353 4367 2353 Size: 2014 3624 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 3940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2218 3381 4953 2218 Size: 1163 1572 2903 Fragments arranged by size: 2903 1572 1163 ApaI G_GGCC'C Cuts at: 2507 2507 Size: 5638 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2684 2963 1870 Size: 73 741 279 4545 Fragments arranged by size: 4545 741 279 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2361 2372 2959 4414 618 Size: 325 217 627 574 11 587 1455 1842 Fragments arranged by size: 1842 1455 627 587 574 325 217 11 BanII G_rGCy'C Cuts at: 1391 2507 2685 2957 1391 Size: 1116 178 272 4072 Fragments arranged by size: 4072 1116 272 178 BbeI G_GCGC'C Cuts at: 2376 2376 Size: 5638 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2549 4249 136 Size: 107 122 71 631 605 877 1700 1525 Fragments arranged by size: 1700 1525 877 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 2507 2693 2778 3699 4855 1790 Size: 717 186 85 921 1156 2573 Fragments arranged by size: 2573 1156 921 717 186 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 3369 4327 Size: 416 53 83 399 24 1454 498 958 Cuts at: 4327 442 Size: 1753 Fragments arranged by size: 1753 1454 958 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2884 4298 1239 Size: 1565 80 1414 2579 Fragments arranged by size: 2579 1565 1414 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 2340 4301 882 Size: 617 627 214 1961 2219 Fragments arranged by size: 2219 1961 627 617 214 BsaAI yAC'GTr Cuts at: 493 1204 2206 2671 3429 493 Size: 711 1002 465 758 2702 Fragments arranged by size: 2702 1002 758 711 465 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2373 2430 3445 Size: 53 83 186 936 482 358 57 1015 Cuts at: 3445 3827 4462 275 Size: 382 635 1451 Fragments arranged by size: 1451 1015 936 635 482 382 358 186 83 57 53 BsaWI w'CCGG_w Cuts at: 2125 2364 4071 4469 4743 4890 5301 2125 Size: 239 1707 398 274 147 411 2462 Fragments arranged by size: 2462 1707 411 398 274 239 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2278 2308 2660 Size: 30 165 30 633 30 212 30 352 Cuts at: 2660 2690 1178 Size: 30 4126 Fragments arranged by size: 4126 633 352 212 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2573 2678 2866 2869 2887 1186 Size: 946 441 105 188 3 18 3937 Fragments arranged by size: 3937 946 441 188 105 18 3 BseYI C'CCAG_C Cuts at: 2495 2716 2909 4841 5337 2495 Size: 221 193 1932 496 2796 Fragments arranged by size: 2796 1932 496 221 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 1163 Size: 573 768 4297 Fragments arranged by size: 4297 768 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3088 3849 4877 1540 Size: 275 186 418 669 761 1028 2301 Fragments arranged by size: 2301 1028 761 669 418 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 2205 2670 3699 3784 4855 2144 Size: 61 465 1029 85 1071 2927 Fragments arranged by size: 2927 1071 1029 465 85 61 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2205 2507 2670 2685 2693 2778 Size: 399 354 61 302 163 15 8 85 Cuts at: 2778 2957 3699 3784 4855 1391 Size: 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 302 179 163 85 85 61 15 8 BspEI T'CCGG_A Cuts at: 2125 5301 2125 Size: 3176 2462 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 2334 2364 2414 2418 2614 4282 Size: 110 377 147 30 50 4 196 1668 Cuts at: 4282 4469 1700 Size: 187 2869 Fragments arranged by size: 2869 1668 377 196 187 147 110 50 30 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstXI CCAn_nnnn'nTGG Cuts at: 2560 2560 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2280 2968 3335 3720 3737 5189 5275 5287 Size: 308 688 367 385 17 1452 86 12 Cuts at: 5287 5633 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 688 385 367 346 308 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 513 Size: 552 939 51 4096 Fragments arranged by size: 4096 939 552 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 2044 3154 3948 3968 2044 Size: 1110 794 20 3714 Fragments arranged by size: 3714 1110 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2189 2416 Size: 54 1425 50 275 18 177 182 227 Cuts at: 2416 2838 3085 3974 5603 8 Size: 422 247 889 1629 43 Fragments arranged by size: 1629 1425 889 422 275 247 227 182 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2416 3085 1537 Size: 275 604 669 4090 Fragments arranged by size: 4090 669 604 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 3568 1353 Size: 771 1444 3423 Fragments arranged by size: 3423 1444 771 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2804 2884 3701 4298 5085 5353 1239 Size: 393 1172 80 817 597 787 268 1524 Fragments arranged by size: 1524 1172 817 787 597 393 268 80 EcoO109I rG'GnC_Cy Cuts at: 2323 2504 2606 2638 2323 Size: 181 102 32 5323 Fragments arranged by size: 5323 181 102 32 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 2376 4785 1678 Size: 698 2409 2531 Fragments arranged by size: 2531 2409 698 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2335 2339 2367 2371 4279 Size: 32 135 32 763 4 28 4 1908 Cuts at: 4279 4311 4353 4385 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1908 763 135 42 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2467 2982 3228 669 Size: 988 281 529 515 246 3079 Fragments arranged by size: 3079 988 529 515 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KasI G'GCGC_C Cuts at: 2372 2372 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 4752 4936 1305 Size: 873 2574 184 2007 Fragments arranged by size: 2574 2007 873 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2864 3596 3955 4114 518 Size: 648 1698 732 359 159 2042 Fragments arranged by size: 2042 1698 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2737 2799 3054 3731 4879 5124 1606 Size: 1131 62 255 677 1148 245 2120 Fragments arranged by size: 2120 1148 1131 677 255 245 62 NaeI GCC'GGC Cuts at: 2189 2336 2420 2189 Size: 147 84 5407 Fragments arranged by size: 5407 147 84 NarI GG'CG_CC Cuts at: 2373 2373 Size: 5638 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NgoMIV G'CCGG_C Cuts at: 2187 2334 2418 2187 Size: 147 84 5407 Fragments arranged by size: 5407 147 84 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 2508 1348 Size: 1160 4478 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5638 PfoI T'CCnGG_A Cuts at: 2026 2026 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PmlI CAC'GTG Cuts at: 2206 2206 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2323 Size: 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PspOMI G'GGCC_C Cuts at: 2503 2503 Size: 5638 PspXI vC'TCGA_Gb Cuts at: 2684 2684 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 PvuII CAG'CTG Cuts at: 2737 2799 2737 Size: 62 5576 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SanDI GG'GwC_CC Cuts at: 2323 2323 Size: 5638 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2986 4121 4802 4993 826 Size: 219 1941 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2374 2374 Size: 5638 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 3742 4643 4905 820 Size: 197 926 741 1058 901 262 1553 Fragments arranged by size: 1553 1058 926 901 741 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 3077 513 Size: 552 939 1073 3074 Fragments arranged by size: 3074 1073 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 3665 3682 3835 4020 4465 325 Size: 1255 1128 957 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1128 957 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3884 5596 96 Size: 274 80 33 51 1443 1907 1712 138 Fragments arranged by size: 1907 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2278 2310 2660 2692 5598 5630 2036 Size: 32 210 32 350 32 2906 32 2044 Fragments arranged by size: 2906 2044 350 210 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2497 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 2684 1943 Size: 741 4897 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI