(Linear) (Six-base) MAPSORT of: KIBB6481.seq Check: 6957 from: 1 to: 933 KIBB6481.orf With 182 enzymes: SgfI * July 3, 2012 16:22 .. AgeI A'CCGG_T Cuts at: 0 359 933 Size: 359 574 AhdI GACnn_n'nnGTC Cuts at: 0 348 933 Size: 348 585 AleI CACnn'nnGTG Cuts at: 0 859 933 Size: 859 74 AlwNI CAG_nnn'CTG Cuts at: 0 213 933 Size: 213 720 ApaI G_GGCC'C Cuts at: 0 502 933 Size: 502 431 AvaI C'yCGr_G Cuts at: 0 679 933 Size: 679 254 BanI G'GyrC_C Cuts at: 0 356 367 933 Size: 356 11 566 Fragments arranged by size: 566 356 11 BanII G_rGCy'C Cuts at: 0 502 680 933 Size: 502 178 253 Fragments arranged by size: 502 253 178 BbeI G_GCGC'C Cuts at: 0 371 933 Size: 371 562 BglI GCCn_nnn'nGGC Cuts at: 0 544 933 Size: 544 389 Bme1580I G_kGCm'C Cuts at: 0 502 688 773 933 Size: 502 186 85 160 Fragments arranged by size: 502 186 160 85 BmrI ACTGGGnnnn_n' Cuts at: 0 866 933 Size: 866 67 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 799 879 933 Size: 799 80 54 Fragments arranged by size: 799 80 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 121 335 933 Size: 121 214 598 Fragments arranged by size: 598 214 121 BsaAI yAC'GTr Cuts at: 0 201 666 933 Size: 201 465 267 Fragments arranged by size: 465 267 201 BsaHI Gr'CG_yC Cuts at: 0 10 368 425 933 Size: 10 358 57 508 Fragments arranged by size: 508 358 57 10 BsaWI w'CCGG_w Cuts at: 0 120 359 933 Size: 120 239 574 Fragments arranged by size: 574 239 120 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 31 61 273 303 655 685 933 Size: 31 30 212 30 352 30 248 Fragments arranged by size: 352 248 212 31 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 127 568 673 861 864 882 933 Size: 127 441 105 188 3 18 51 Fragments arranged by size: 441 188 127 105 51 18 3 BseYI C'CCAG_C Cuts at: 0 490 711 904 933 Size: 490 221 193 29 Fragments arranged by size: 490 221 193 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 933 Size: 499 434 BsiEI CG_ry'CG Cuts at: 0 414 933 Size: 414 519 BsiHKAI G_wGCw'C Cuts at: 0 139 200 665 933 Size: 139 61 465 268 Fragments arranged by size: 465 268 139 61 Bsp1286I G_dGCh'C Cuts at: 0 139 200 502 665 680 688 773 933 Size: 139 61 302 163 15 8 85 160 Fragments arranged by size: 302 163 160 139 85 61 15 8 BspEI T'CCGG_A Cuts at: 0 120 933 Size: 120 813 BsrFI r'CCGG_y Cuts at: 0 182 329 359 409 413 609 933 Size: 182 147 30 50 4 196 324 Fragments arranged by size: 324 196 182 147 50 30 4 BstXI CCAn_nnnn'nTGG Cuts at: 0 555 933 Size: 555 378 BstYI r'GATC_y Cuts at: 0 275 933 Size: 275 658 BtgI C'CryG_G Cuts at: 0 50 933 Size: 50 883 BtsI GCAGTG_nn' Cuts at: 0 39 933 Size: 39 894 EaeI y'GGCC_r Cuts at: 0 2 184 411 833 933 Size: 2 182 227 422 100 Fragments arranged by size: 422 227 182 100 2 EagI C'GGCC_G Cuts at: 0 411 933 Size: 411 522 EarI CTCTTCn'nnn_ Cuts at: 0 119 933 Size: 119 814 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 799 879 933 Size: 799 80 54 Fragments arranged by size: 799 80 54 EcoO109I rG'GnC_Cy Cuts at: 0 318 499 601 633 933 Size: 318 181 102 32 300 Fragments arranged by size: 318 300 181 102 32 HaeII r_GCGC'y Cuts at: 0 371 933 Size: 371 562 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 330 334 362 366 933 Size: 330 4 28 4 567 Fragments arranged by size: 567 330 28 4 4 HincII GTy'rAC Cuts at: 0 462 933 Size: 462 471 KasI G'GCGC_C Cuts at: 0 367 933 Size: 367 566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 173 933 Size: 173 760 MslI CAynn'nnrTG Cuts at: 0 859 933 Size: 859 74 MspA1I CmG'CkG Cuts at: 0 732 794 933 Size: 732 62 139 Fragments arranged by size: 732 139 62 NaeI GCC'GGC Cuts at: 0 184 331 415 933 Size: 184 147 84 518 Fragments arranged by size: 518 184 147 84 NarI GG'CG_CC Cuts at: 0 368 933 Size: 368 565 NgoMIV G'CCGG_C Cuts at: 0 182 329 413 933 Size: 182 147 84 520 Fragments arranged by size: 520 182 147 84 PasI CC'CwG_GG Cuts at: 0 503 933 Size: 503 430 PfoI T'CCnGG_A Cuts at: 0 21 933 Size: 21 912 PmlI CAC'GTG Cuts at: 0 201 933 Size: 201 732 PpuMI rG'GwC_Cy Cuts at: 0 318 933 Size: 318 615 PspOMI G'GGCC_C Cuts at: 0 498 933 Size: 498 435 PspXI vC'TCGA_Gb Cuts at: 0 679 933 Size: 679 254 PvuII CAG'CTG Cuts at: 0 732 794 933 Size: 732 62 139 Fragments arranged by size: 732 139 62 SanDI GG'GwC_CC Cuts at: 0 318 933 Size: 318 615 SfoI GGC'GCC Cuts at: 0 369 933 Size: 369 564 SmlI C'TyrA_G Cuts at: 0 679 933 Size: 679 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 703 933 Size: 703 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 31 63 273 305 655 687 933 Size: 31 32 210 32 350 32 246 Fragments arranged by size: 350 246 210 32 32 32 31 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 492 933 Size: 492 441 XhoI C'TCGA_G Cuts at: 0 679 933 Size: 679 254 Enzymes that do cut: AgeI AhdI AleI AlwNI ApaI AvaI BanI BanII BbeI BglI Bme1580I BmrI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstXI BstYI BtgI BtsI EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI PfoI PmlI PpuMI PspOMI PspXI PvuII SanDI SfoI SmlI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XmaI XmnI ZraI